Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_008463114,8650TC32.9% 1368.5 / inf 758Y529H (TAC→CAC) vgrG1btype VI secretion system spike protein VgrG1b
Reads supporting (aligned to +/- strand):  ref base T (253/251);  new base C (119/128);  total (374/384)
Fisher's exact test for biased strand distribution p-value = 6.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.70e-02
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 763 total aligned reads displayed.

GCGGCACGCCGGCCAACTTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAAGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATCGGCGTCGACCGCACCGAGAAGGTCGGTAACAACGAGAAGATCAGTATCGGCGCGAACCGCACCGAGGACGTCGGCAGCAAC  >  NC_008463/114716‑115014
                                                                                                                                                     |                                                                                                                                                     
  ggcACGCCGGCCAACTTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAAGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTac                                                                                                                                                     <  1:10209625/150‑1 (MQ=255)
  ggcACGCCGGCCAACCTCAACGCGCTCCGCATGGAGGACACGCAGGGCGCCGAGCAGGTGTACCTCCATGCCGCGAAGAACCAGGACAGCGACGACGCCAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTac                                                                                                                                                     <  2:10471174/150‑1 (MQ=14)
   gcACGCCGGCCAACTTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCacg                                                                                                                                                    >  2:326197/1‑150 (MQ=255)
     aCGCCGGCCAACTTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCacgac                                                                                                                                                  <  1:6278010/150‑1 (MQ=255)
     aCGCCGGCCAACTTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAAGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTacgac                                                                                                                                                  <  2:7263558/150‑1 (MQ=255)
         cGGCCAACTTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAAGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACgaga                                                                                                                                              >  1:7257793/1‑150 (MQ=255)
          gtccAACTTCACCTAGATCCGCTTGGAGGACAAGAAGGGCGTCGTGCAGTTGTTCATCCATGCCGAGATGAACCAGGACTTCGTGGTCGAGAAGGACGAGTGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGAc                                                                                                                                             <  2:5212330/148‑1 (MQ=14)
            ccAACTTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAAGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACgg                                                                                                                                           <  1:7426543/150‑1 (MQ=255)
             cAACTTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAAGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGt                                                                                                                                          >  1:2744155/1‑150 (MQ=255)
               aCTTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGc                                                                                                                                        >  2:9857611/1‑150 (MQ=255)
                cTTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAAGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCa                                                                                                                                       >  1:3134324/1‑150 (MQ=255)
                  tCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCAtg                                                                                                                                     <  1:7699025/150‑1 (MQ=255)
                  tCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGACGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCAtg                                                                                                                                     <  2:5366173/150‑1 (MQ=255)
                  tCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAAGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCAtg                                                                                                                                     >  2:3056850/1‑150 (MQ=255)
                   cAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCAtgt                                                                                                                                    <  1:5101346/150‑1 (MQ=255)
                   cAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAAGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCAtgt                                                                                                                                    >  1:7913101/1‑150 (MQ=255)
                   cAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAAGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCAtgt                                                                                                                                    <  2:1314045/150‑1 (MQ=255)
                    aaCGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCAtgtg                                                                                                                                   <  1:4721113/150‑1 (MQ=255)
                     aCGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAAGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGa                                                                                                                                  >  1:9435009/1‑150 (MQ=255)
                       gagaTCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAg                                                                                                                                >  1:4911975/1‑150 (MQ=255)
                         gaTCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAAGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCa                                                                                                                              >  1:2041984/1‑150 (MQ=255)
                         gaTCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAAGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCa                                                                                                                              <  1:5565065/150‑1 (MQ=255)
                         gaTCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAAGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCa                                                                                                                              >  1:8755095/1‑150 (MQ=255)
                            ccGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGa                                                                                                                           <  1:9792331/150‑1 (MQ=255)
                            ccGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGa                                                                                                                           <  1:5963260/150‑1 (MQ=255)
                            ccGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGa                                                                                                                           <  2:2995555/150‑1 (MQ=255)
                              gCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATc                                                                                                                         <  1:339551/150‑1 (MQ=255)
                              gCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATc                                                                                                                         >  2:957516/1‑150 (MQ=255)
                               cATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAAGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGACGCACGATCg                                                                                                                        <  1:3316710/150‑1 (MQ=255)
                                aTGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGc                                                                                                                       <  2:9390506/150‑1 (MQ=255)
                                 tGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAAGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCCCGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGAACGCa                                                                                                                      >  2:677092/1‑150 (MQ=255)
                                 tGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAAGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCa                                                                                                                      <  2:4748080/150‑1 (MQ=255)
                                 tGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAAGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCa                                                                                                                      <  1:1129495/150‑1 (MQ=255)
                                 tGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAAGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCa                                                                                                                      <  2:3411442/150‑1 (MQ=255)
                                   gaggaCAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCAcc                                                                                                                    >  2:8130974/1‑150 (MQ=255)
                                   gaggaCAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCAcc                                                                                                                    <  1:2423997/150‑1 (MQ=255)
                                   gaggaCAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAAGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCAcc                                                                                                                    >  1:3796735/1‑150 (MQ=255)
                                        cAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAAGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGAc                                                                                                               <  1:8315519/150‑1 (MQ=255)
                                         aagaagGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGAcc                                                                                                              >  2:8794103/1‑150 (MQ=255)
                                          agaagGGCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCg                                                                                                             >  1:2149276/1‑150 (MQ=255)
                                          agaagGGCGCCGAGCAGATGTTCATCCATGCCGAGAAGAACCAGGACATCGAGGTCGATAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAATACCAACGACTACGACGAGACGGAGCATGTGAAGCACGATCGCACCGAGACCg                                                                                                             >  2:2640091/1‑150 (MQ=14)
                                            aagGGCGCCGAGCAGTTGTTCATCCATGCCGAGAAGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTg                                                                                                           <  1:1333284/150‑1 (MQ=255)
                                             agGGCGCCGAGCAGTTGTTCATCCATGCCGTGAGGAACCAGGACTTCGTGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTgg                                                                                                          <  2:6300241/150‑1 (MQ=11)
                                              gggCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATNGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGa                                                                                                         <  1:5799329/150‑1 (MQ=21)
                                              gggCGCCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGa                                                                                                         >  2:7835615/1‑150 (MQ=255)
                                                  gcCGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGacaac                                                                                                     <  2:8598687/150‑1 (MQ=255)
                                                  gcCGAGCAGTTGTTCATCCATGCCGAGAAGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGacaac                                                                                                     <  2:2220637/150‑1 (MQ=255)
                                                    cGAGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGAcaacaa                                                                                                   <  2:4229429/150‑1 (MQ=255)
                                                     gAGCAGTTGTTCATCCATGCCGTGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACaacaac                                                                                                  >  2:3943628/1‑150 (MQ=255)
                                                      aGCAGTTGTTCATCCATGCCGAGAAGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACg                                                                                                 >  2:9119289/1‑150 (MQ=255)
                                                      aGCAGTTGTTCATCCATGCCGAGAAGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACg                                                                                                 >  1:3649981/1‑150 (MQ=255)
                                                       gCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACga                                                                                                >  2:10169711/1‑150 (MQ=255)
                                                       gCAGTTGTTCATCCATGCCGAGAAGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACga                                                                                                <  2:1632102/150‑1 (MQ=255)
                                                         aGTTGTTCATCCATGCCGAGAAGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACgaga                                                                                              <  1:2308770/150‑1 (MQ=255)
                                                         aGTTGTTCATCCATGCCGAGAAGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACgaga                                                                                              <  1:7996946/150‑1 (MQ=255)
                                                          gttgttCATCCATGCCGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGAc                                                                                             >  1:1841229/1‑150 (MQ=255)
                                                          gttgttCATCCATGCCGAGAAGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGAc                                                                                             >  1:5275702/1‑150 (MQ=255)
                                                           ttgttCATCCATGCCGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGAcc                                                                                            <  1:10830290/150‑1 (MQ=255)
                                                           ttgttCATCCATGCCGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGAcc                                                                                            >  2:8398230/1‑150 (MQ=255)
                                                             gttCATCCATGCCGAGAAGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCAt                                                                                          <  1:671238/150‑1 (MQ=255)
                                                              gccctCCATGCCGAGAAGAACCCGGACACCGAGGTCGAGACCGCCGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGCCGAGACGGTGCATGTGAAGCACGCTCGCACCGAGACCGTGGACAACAACGAGCCCATa                                                                                         <  2:3869950/146‑1 (MQ=14)
                                                               tCATCCATGCCGAGAAGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATaa                                                                                        <  1:1738895/150‑1 (MQ=255)
                                                                  tCCATGCCGAGAAGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCa                                                                                     <  2:6073176/150‑1 (MQ=255)
                                                                   ccATGCCGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCAccgt                                                                                    >  1:4141642/1‑148 (MQ=255)
                                                                    cATGCCGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCAccgtg                                                                                   >  2:3578825/1‑147 (MQ=255)
                                                                    cATGCCGAGAAGACCCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGCTCGCACCGAGACCGTGGACAACAACGAGACCATACCCATc                                                                                   <  2:7565694/150‑1 (MQ=255)
                                                                     aTGCCGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCAccgtgc                                                                                  >  2:657825/1‑146 (MQ=255)
                                                                     aTGCCGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCAccgtgc                                                                                  <  1:888924/150‑5 (MQ=255)
                                                                     aTGCCGAGAAGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATCg                                                                                  >  1:349934/1‑150 (MQ=255)
                                                                     aTGCCGAGAAGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATCg                                                                                  >  2:89018/1‑150 (MQ=255)
                                                                       gCCGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCAccgtgcat                                                                                <  1:10655273/150‑7 (MQ=1)
                                                                              agaaCCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATCGGCGTCGAcc                                                                         <  2:5695238/150‑1 (MQ=255)
                                                                              agaaCCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATCGGCGTCGAcc                                                                         <  2:588266/150‑1 (MQ=255)
                                                                              agaaCCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATCGGCGTCGAcc                                                                         <  2:5030465/150‑1 (MQ=255)
                                                                               gaaCCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATCGGCGACGACCg                                                                        >  2:11087531/1‑150 (MQ=255)
                                                                                 aCCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATCGGCGTCGACCGCa                                                                      >  1:7126788/1‑150 (MQ=255)
                                                                                     ggACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATCGGCGTCGACCGCACCga                                                                  >  1:9615839/1‑150 (MQ=255)
                                                                                     ggACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACAACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATCGTCGTCGACCGCACCga                                                                  <  2:2094708/150‑1 (MQ=255)
                                                                                      gACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATCGGCGTCGACCGCACCgag                                                                 <  2:6970166/150‑1 (MQ=255)
                                                                                        cATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCCCCGAGACCGTGGACAACAACGAGACCATAACCATCGGCGTCGACCGCACCGAGaa                                                               <  2:2516074/150‑1 (MQ=255)
                                                                                           cGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATCGGCGTCGACCGCACCGAGAAGGt                                                            <  2:1941682/150‑1 (MQ=255)
                                                                                           cGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATCGGCGTCGACCGCACCGAGAAGGt                                                            <  1:9165600/150‑1 (MQ=255)
                                                                                             aGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATCGGCGTCGACCGCACCGAGAAGGTCg                                                          >  1:8577203/1‑150 (MQ=255)
                                                                                              ggTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATCGGCGTCGACCGCACCGAGAAGGTCgg                                                         >  2:1441240/1‑150 (MQ=255)
                                                                                                tCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATCGGCGTCGACCGCACCGAGAAGGTCGGTa                                                       >  1:7831459/1‑150 (MQ=255)
                                                                                                    gaACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATCGGCGTCGACCGCACCGAGAAGGTCGGTaacaa                                                   <  1:437930/150‑1 (MQ=255)
                                                                                                      acgacgAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATCGGCGTCGACCGCACCGAGAAGGTCGGTAACAACg                                                 <  2:10905767/150‑1 (MQ=255)
                                                                                                        gacgaGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATCGGCGTCGACCGCACCGAGAAGGTCGGTAACAACgag                                               <  2:10408962/150‑1 (MQ=255)
                                                                                                         acgaGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACCACAACGAGACCATAACCATCGGCGTCGACCGCACCGAGAAGGTCGGCACCAACgaga                                              >  2:2788170/1‑150 (MQ=255)
                                                                                                           gagaGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATCGGCGTCGACCGCACCGAGAAGGTCGGTAACAACGagaag                                            >  1:4980902/1‑150 (MQ=255)
                                                                                                                  aCTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATCGGCGTCGACCGCACCGAGAAGGTCGGTAACAACGAGAAGATCAGTa                                     <  2:4611580/150‑1 (MQ=255)
                                                                                                                             cACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATCGGCGTCGACCGCACCGAGAAGGTCGGTAACAACGAGAAGATCAGTATCGGCGCGAAc                          >  1:8457953/1‑150 (MQ=255)
                                                                                                                               cGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATCGGCGTCGACCGCACCGAGAAGGTCGGTAACAACGAGAAGATCAGTATCGGCGCGAACCg                        >  1:6497066/1‑150 (MQ=255)
                                                                                                                                 aCCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATCGGCGTCGACCGCACCGAGAAGGTCGGTAACAACGAGAAGATCAGTATCGGCGCGAACCGCa                      <  1:9335755/150‑1 (MQ=255)
                                                                                                                                 aCCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATCGGCGTCGACCGCACCGAGAAGGTCGGTAACAACGAGAAGATCAGTATCGGCGCGAACCGCa                      >  1:2951998/1‑150 (MQ=255)
                                                                                                                                 aCCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATCGGCGTCGACCGCACCGAGAAGGTCGGTAACAACGAGAAGATCAGTATCGGCGCGAACCGCa                      <  1:1488632/150‑1 (MQ=255)
                                                                                                                                   cGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATCGGCGTCGACCGCACCGAGAAGGTCGGTAACAACGAGAAGATCAGTATCGGCGCGAACCGCAcc                    <  1:6033623/150‑1 (MQ=255)
                                                                                                                                        cAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATCGGCGTCGACCGCACCGAGAAGGTCGGTAACAACGAGAAGATCAGTATCGGCGCGAACCGCACCGAGGa               >  1:10018367/1‑150 (MQ=255)
                                                                                                                                         aaGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATCGGCGTCGACCGCACCGAGAAGGTCGGTAACAACGAGAAGATCAGTATCGGCGCGAACCGCACCGAGGAc              >  1:9971567/1‑150 (MQ=255)
                                                                                                                                               aTCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATCGGCGTCGACCGCACCGAGAAGGTCGGTAACAACGAGAAGATCAGTATCGGCGCGAACCGCACCGAGGACGTCGgc        <  2:8457955/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GCGGCACGCCGGCCAACTTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCGAGCAGTTGTTCATCCATGCCGAGAAGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGGACCAAGACCATCGACTACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATAACCATCGGCGTCGACCGCACCGAGAAGGTCGGTAACAACGAGAAGATCAGTATCGGCGCGAACCGCACCGAGGACGTCGGCAGCAAC  >  NC_008463/114716‑115014

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑