BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_008463	5,931,608	(T)_6→5	intergenic (‑27/‑151)	PA14_RS27130 ← / → PA14_RS27135	LysR family transcriptional regulator/MFS transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_008463	5,931,603	0	T	.	100.0%	2237.6 / NA	455	intergenic (‑22/‑156)	PA14_RS27130/PA14_RS27135	LysR family transcriptional regulator/MFS transporter
Reads supporting (aligned to +/- strand): ref base T (0/0); new base . (171/284); total (171/284)

Only 100 of 487 total aligned reads displayed.

GATTTCCTCCTCCAGCGCCTGGATCTGCTGGCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTATTTAGGTGTTTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGAAATCCAGGTGGCGCGGCCTCGTTGTGCCTGCTCGCCACCGTCCCCA  >  NC_008463/5931453‑5931748

 aTTTCCTCCTCCAGCGCCTGGATCTGCTGGCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTGtt                                                                                                                                                  >  1:6412443/1‑150 (MQ=255)

 aTTTCCTCCTCCAGCGCCTGGATCTGCTGGCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTGtt                                                                                                                                                  <  1:2001039/150‑1 (MQ=255)

   ttCCTCCTCCAGCGCCTGGATCTGCTGGCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTGtttt                                                                                                                                                >  1:3444607/1‑150 (MQ=255)

      cccctccCGCGCCTCGATCTGCAGGCTCAACGTCGGCTGGGAGATCCCCAGGCGCTCTGCGGCGCGGCCGAAGTTCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTGtttttac                                                                                                                                             <  2:6548450/148‑3 (MQ=255)

       tcctccAGCGCCTGGATCTGCTGGCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGACGTCAAGCTCCTCGGCGACGGCGATGAACTAGCGCAGGTGCCTGAGTTCCAAGCGGCCACTCCTA‑TGATGTGtttttact                                                                                                                                            >  2:6882112/1‑147 (MQ=255)

        cctccAGCGCCTGGATCTGCTGGCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTGtttttacat                                                                                                                                           <  1:7154432/150‑5 (MQ=255)

         ctccAGCGCCTGGATCTGCTGGCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACata                                                                                                                                         <  2:3770007/150‑1 (MQ=255)

           ccAGCGCCTGGATCTGCTGGCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATc                                                                                                                                       <  2:5014527/150‑1 (MQ=255)

            cAGCGCCTGGATCTGCTGGCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCa                                                                                                                                      >  1:2208856/1‑150 (MQ=255)

             aGCGCCTGGATCTGCTGGCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCaa                                                                                                                                     >  2:6784982/1‑150 (MQ=255)

              gcgcCTGGATCTGCTGGCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCact                                                                                                                                    <  1:7838681/150‑3 (MQ=255)

               cgcCTGGATCTGCTGGCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATc                                                                                                                                   >  2:9243369/1‑150 (MQ=255)

                   tGGATCTGCTGGCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGACGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCGA‑TTAGGTG‑TTTTTACATATCAATCgtgt                                                                                                                               >  1:5591609/1‑150 (MQ=255)

                   tGGATCTGCTGGCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCgtgt                                                                                                                               <  1:8829342/150‑1 (MQ=255)

                   tGGATCTGCTGGCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCgtgt                                                                                                                               >  1:5858339/1‑150 (MQ=255)

                    ggATCTGCTGGCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTc                                                                                                                              <  2:6243920/150‑1 (MQ=255)

                     gATCTGCTGGCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCg                                                                                                                             >  1:2499020/1‑150 (MQ=255)

                          gctgGCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCaa                                                                                                                        <  1:1865458/150‑1 (MQ=255)

                          gctgGCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCaa                                                                                                                        >  2:6922356/1‑150 (MQ=255)

                          gctgGCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCaa                                                                                                                        <  1:4268469/150‑1 (MQ=255)

                           ctgGCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAat                                                                                                                       <  2:341709/150‑1 (MQ=255)

                            tgGCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAata                                                                                                                      <  2:10129677/150‑1 (MQ=255)

                             ggCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAatat                                                                                                                     >  1:1606763/1‑150 (MQ=255)

                               cTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAatatat                                                                                                                   <  1:8933178/150‑1 (MQ=255)

                                 cAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTg                                                                                                                 >  2:5627436/1‑150 (MQ=255)

                                  aaCGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTgg                                                                                                                >  1:9108448/1‑150 (MQ=255)

                                    cggcggGGGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGGGCAGCTCCTCGGCGACGGCGATGAAATTGCGCCGGTGCCTGAGTTCCAAGCGGACCCTCCTA‑TTAGGTG‑GTTTTACATATCAATCGTGTCGAGCAATATATTGGat                                                                                                              >  2:4131011/1‑149 (MQ=255)

                                        ggcTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCa                                                                                                          <  1:991829/150‑1 (MQ=255)

                                         gcTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCat                                                                                                         >  1:4234668/1‑150 (MQ=255)

                                         gcTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCat                                                                                                         <  1:7744855/150‑1 (MQ=255)

                                           tGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCatat                                                                                                       <  2:9748178/150‑1 (MQ=255)

                                           tGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTACTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATAGCAATCGTGTCGATCAATATATTGGAACTCatat                                                                                                       >  1:7477290/1‑150 (MQ=255)

                                            gggAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGTCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCTATCAATATATTGGAAATCAtata                                                                                                      >  2:10023132/1‑150 (MQ=255)

                                            gggAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCAtata                                                                                                      >  2:10284138/1‑150 (MQ=255)

                                            gggAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCAtata                                                                                                      <  2:8954171/150‑1 (MQ=255)

                                                gaTCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAAcc                                                                                                  >  1:3177149/1‑150 (MQ=255)

                                                 aTCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACct                                                                                                 <  1:3595755/150‑1 (MQ=255)

                                                  tCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACcta                                                                                                <  1:335985/150‑1 (MQ=255)

                                                  tCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACcta                                                                                                <  2:8324732/150‑1 (MQ=255)

                                                     ccAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACctatct                                                                                             <  2:4839646/150‑1 (MQ=255)

                                                     ccAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACctatct                                                                                             <  1:2984603/150‑1 (MQ=255)

                                                      cAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACctatcta                                                                                            >  2:214067/1‑150 (MQ=255)

                                                       aGGCGCTCGGCGGCGCGGCCGACGAGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACctatctat                                                                                           <  1:4037869/150‑1 (MQ=255)

                                                         gcgcTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTatat                                                                                         <  2:8171500/150‑1 (MQ=255)

                                                          cgcTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGTAGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTGGATCAATATTTTGGAAATCATATAAACCAATCTATATc                                                                                        <  2:11045900/150‑1 (MQ=255)

                                                          cgcTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATc                                                                                        <  1:8716727/150‑1 (MQ=255)

                                                           gcTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATCTcc                                                                                       <  1:5482886/150‑1 (MQ=255)

                                                           gcTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATcc                                                                                       >  1:7391739/1‑150 (MQ=255)

                                                            cTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCt                                                                                      >  1:803841/1‑150 (MQ=255)

                                                              cggcggCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCttt                                                                                    <  2:5395243/150‑1 (MQ=255)

                                                                gcggcgcggcCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGc                                                                                  <  1:6212941/150‑1 (MQ=255)

                                                                 cggcgcggcCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGcc                                                                                 <  2:9757031/150‑1 (MQ=255)

                                                                  ggcgcggcCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCca                                                                                <  2:3102265/150‑1 (MQ=255)

                                                                      cggcCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATa                                                                            <  1:8492414/150‑1 (MQ=255)

                                                                        gcCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAAc                                                                          <  2:2375855/150‑1 (MQ=255)

                                                                        gcCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAAc                                                                          >  2:10388673/1‑150 (MQ=255)

                                                                          cGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCt                                                                        <  1:3812027/150‑1 (MQ=255)

                                                                          cGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCt                                                                        >  1:10000545/1‑150 (MQ=255)

                                                                           gAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTc                                                                       >  1:4715190/1‑150 (MQ=255)

                                                                              gTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCcaa                                                                    <  2:7112947/150‑1 (MQ=255)

                                                                                 cAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCcaacaa                                                                 <  1:9423327/150‑1 (MQ=255)

                                                                                 cAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCcaacaa                                                                 <  1:5262806/150‑1 (MQ=255)

                                                                                   gCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACg                                                               <  2:1164476/150‑1 (MQ=255)

                                                                                    ctcctcGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGa                                                              <  1:10231681/150‑1 (MQ=255)

                                                                                    ctcctcGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGa                                                              <  1:8619882/150‑1 (MQ=255)

                                                                                      cctcGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATCTTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGAcc                                                            >  2:3257848/1‑150 (MQ=255)

                                                                                      cctcGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGAcc                                                            >  1:6657575/1‑150 (MQ=255)

                                                                                          ggcgacggcgaTGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATAACAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCtt                                                        >  2:4265998/1‑150 (MQ=255)

                                                                                             gacggcgaTGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTc                                                     >  2:2670432/1‑150 (MQ=255)

                                                                                             gacggcgaTGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTc                                                     <  1:326118/150‑1 (MQ=255)

                                                                                              acggcgaTGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTcc                                                    >  2:3990402/1‑150 (MQ=255)

                                                                                                ggcgaTGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCt                                                  <  1:9733337/150‑1 (MQ=255)

                                                                                                    aTGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGaaa                                              <  2:9372914/150‑1 (MQ=255)

                                                                                                    aTGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGaaa                                              >  2:6449875/1‑150 (MQ=255)

                                                                                                        aaTAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGAAATCCa                                          <  1:10356994/150‑1 (MQ=255)

                                                                                                        aaTAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGAAATCCa                                          <  1:6372768/150‑1 (MQ=255)

                                                                                                             cgcAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGAAATCCAGGTgg                                     <  2:5945544/150‑1 (MQ=255)

                                                                                                              gcAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGAAATCCAGGTGgc                                    <  2:1695329/150‑1 (MQ=255)

                                                                                                               cAGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGAAATCCAGGTGgcg                                   >  2:7953556/1‑150 (MQ=255)

                                                                                                                aGGTGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGAAATCCAGGTGgcgc                                  >  2:2230826/1‑150 (MQ=255)

                                                                                                                   tGCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGAAATCCAGGTGGCGCGGc                               >  1:43398/1‑150 (MQ=255)

                                                                                                                   tGCCTGAGTTCCAAGCGGCCCATCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGAAATCCAGGTGGCGCGGc                               <  2:9037226/150‑1 (MQ=255)

                                                                                                                    gCCTGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGAAATCCAGGTGGCGCGGcc                              >  1:5004622/1‑150 (MQ=255)

                                                                                                                       tGAGTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGAAATCCAGGTGGCGCGGCCTCg                           <  2:1340415/150‑1 (MQ=255)

                                                                                                                          gTTCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGAAATCCAGGTGGCGCGGCCTCGTtg                        >  2:7745209/1‑150 (MQ=255)

                                                                                                                            tCCAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGAAATCCAGGTGGCGCGGCCTCGTtgtg                      <  1:5530193/150‑1 (MQ=255)

                                                                                                                              cAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGAAATCCAGGTGGCGCGGCCTCGTTGTGcc                    >  1:11022178/1‑150 (MQ=255)

                                                                                                                              cAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGAAATCCAGGTGGCGCGGCCTCGTTGTGcc                    >  1:6890689/1‑150 (MQ=255)

                                                                                                                              cAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGAAATCCAGGTGGCGCGGCCTCGTTGTGcc                    >  2:7000210/1‑150 (MQ=255)

                                                                                                                              cAAGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGAAATCCAGGTGGCGCGGCCTCGTTGTGcc                    <  1:9598502/150‑1 (MQ=255)

                                                                                                                               aaGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGAAATCCAGGTGGCGCGGCCTCGTTGTGCCt                   >  2:8477188/1‑150 (MQ=255)

                                                                                                                                aGCGGCCCCTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGAAATCCAGGTGGCGCGGCCTCGTTGTGCCTg                  >  1:7642711/1‑150 (MQ=255)

                                                                                                                                aGCGGCCCCTCCTA‑GTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGACATCATATAAACCTATCTATATCCTATGCACCATAACCTCCAACAACGACCCCTTGTCACTGAAATCCAGGTGGCGCGGCCTCGTTGTGCCTg                  >  2:1541618/1‑150 (MQ=255)

                                                                                                                                aGCGGCCCCTCC‑AATTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATAAATAGGAAATCATATAAACCTATCTATATACTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGAAATCCAGGTGGCGCGGCCTCGTTGTGCCTg                  >  2:3506630/1‑150 (MQ=255)

                                                                                                                                      cccTCCTA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGAAATCCTGGTGGCGCGGCCTCGTTGTGCCTGCTCGcc            >  2:9608502/1‑150 (MQ=255)

                                                                                                                                        ctcctA‑TTAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGAAATCCAGGTGGCGCGGCCTCGTTGTGCCTGCTCGCCAc          <  1:9827710/150‑1 (MQ=255)

                                                                                                                                             tattAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGAAATCCAGGTGGCGCGGCCTCGTTGTGCCTGCTCGCCACCGTc      >  2:2904581/3‑150 (MQ=255)

                                                                                                                                               ttAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGAAATCCAGGTGGCGCGGCCTCGTTGTGCCTGCTCGCCACCGTccc    >  2:4604593/1‑150 (MQ=255)

                                                                                                                                                tAGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGAAATCCAGGTGGCGCGGCCTCGTTGTGCCTGCTCGCCACCGTcccc   <  1:1288680/150‑1 (MQ=255)

                                                                                                                                                 aGGTG‑TTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGACACATAACCTCCAACAACGACCCCTTGTCCCTGAAATCCAGGTGGCGCGGCCTCGTTGTGCCTGCTCGCCACCGTCCCCa  >  1:10712488/1‑150 (MQ=255)

GATTTCCTCCTCCAGCGCCTGGATCTGCTGGCTCAACGGCGGCTGGGAGATCCCCAGGCGCTCGGCGGCGCGGCCGAAGTGCAGCTCCTCGGCGACGGCGATGAAATAGCGCAGGTGCCTGAGTTCCAAGCGGCCCCTCCTATTTAGGTGTTTTTTACATATCAATCGTGTCGATCAATATATTGGAAATCATATAAACCTATCTATATCCTTTGCCACATAACCTCCAACAACGACCCCTTGTCCCTGAAATCCAGGTGGCGCGGCCTCGTTGTGCCTGCTCGCCACCGTCCCCA  >  NC_008463/5931453‑5931748

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`