BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_008463	6,070,064	Δ1 bp	pseudogene (533/1086 nt)	PA14_RS27735 →	oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_008463	6,070,063	0	T	.	99.8%	2986.2 / ‑3.6	601	pseudogene (532/1086 nt)	PA14_RS27735	oxidoreductase
Reads supporting (aligned to +/- strand): ref base T (0/0); major base . (288/312); minor base A (1/0); total (289/312)
Fisher's exact test for biased strand distribution p-value = 4.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 628 total aligned reads displayed.

TCCAGAACCGGCGCTTCGACAGCGACTTCCTCGCCGTGGAGGCCGCCCTGCAGGCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGCTTCCGGGCTGTGGTTTGACCTTGGCCCGCAACCTGGTTGACCAAGGCACTGTGCCTGTTTGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCTCGACTATC  >  NC_008463/6069914‑6070210

tCCAGAACCGGCGCTTCGACAGCGACTTCCTCGCCGTGGAGGCCGCCCTGCAGGCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGCt                                                                                                                                                     >  1:1071400/1‑150 (MQ=255)

 ccAGAACCGGCGCTTCGACAGCGACTTCCTCGCCGTGGAGGCCGCCCTGCAGGCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGCtc                                                                                                                                                    <  2:11023783/150‑2 (MQ=255)

  cAGAACCGGCGCTTCGACAGCGACTTCCTCGCCGTGGAGGCCGCCCTGCAGGCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGCtcc                                                                                                                                                   <  1:3897069/150‑3 (MQ=255)

   aGAACCGGCGCTTCGACAGCGACTTCCTCGCCGTGGAGGCCGCCCTGCAGGCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGCtccg                                                                                                                                                  <  2:4257985/150‑4 (MQ=255)

    gAACCGGCGCTTCGACAGCGACTTCCTCGCCGTGGAGGCCGCCCTGCAGGCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGCtccgg                                                                                                                                                 >  2:9920017/1‑146 (MQ=255)

      aCCGGCGCTTCGACAGCGACTTCCTCGCCGTGGAGGCCGCCCTGCAGGCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGc                                                                                                                                              <  1:8108563/150‑1 (MQ=255)

         ggCGCTTCGACAGCGACTTCCTCGCCGTGGAGGCCGCCCTGCAGGCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCtgt                                                                                                                                           >  2:2006031/1‑150 (MQ=255)

         ggCGCTTCGACAGCGACTTCCTCGCCGTGGAGGCCGCCCTGCAGGCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCtgt                                                                                                                                           <  1:2648153/150‑1 (MQ=255)

          gcgcTTCGACAGCGACTTCCTCGCCGTGGAGGCCGCCCTGCAGGCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCtgtg                                                                                                                                          >  1:4871119/1‑150 (MQ=255)

            gcTTCGACAGCGACTTCCTCGCCGTGGAGGCCGCCCTGCAGGCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGt                                                                                                                                        <  2:10052896/150‑1 (MQ=255)

             cTTCGACAGCGACTTCCTCGCCGTGGAGGCCGCCCTGCAGGCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGtt                                                                                                                                       <  1:3292017/150‑1 (MQ=255)

              ttCGACAGCGACTTCCTCGCCGTGGAGGCCGCCCTGCAGGCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGttc                                                                                                                                      <  1:1477618/150‑2 (MQ=255)

              ttCGACAGCGACTGCCTCGCCGTGGAGGCCGCCCTGCAGGCCGGCAGCAGCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTACCGTCCGCAGGTCCGCGACCGCTGGCGCGCACACGACCTGCCCGGC‑TCCGGGCTGTGGttc                                                                                                                                      <  2:7060584/150‑2 (MQ=255)

               tCGACAGCGACTTCCTCGCCGTGGAGGCCGCCCTGCAGGCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGttcg                                                                                                                                     >  2:5927654/1‑148 (MQ=255)

               tCGACAGCGACTTCCTCGCCGTGGAGGCCGCCCTGCAGGCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGttcg                                                                                                                                     >  1:2340035/1‑148 (MQ=255)

                  aCAGCGACTTCCTCGCCGTGGAGGCCGCCCTGCAGGCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGAcc                                                                                                                                  >  2:8251026/1‑150 (MQ=255)

                  aCAGCGACTTCCTCGCCGTGGAGGCCGCCCTGCAGGCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGAcc                                                                                                                                  >  2:2491010/1‑150 (MQ=255)

                   cAGCGACTTCCTCGCCGTGGAGGCCGCCCTGCAGGCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCt                                                                                                                                 <  1:9718641/150‑1 (MQ=255)

                       gACTTCCTCGCCGTGGAGGCCGCCCTGCAGGCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGc                                                                                                                             <  1:1119879/150‑1 (MQ=255)

                       gACTTCCTCGCCGTGGAGGCCGCCCTGCAGGCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGc                                                                                                                             <  2:3952180/150‑1 (MQ=255)

                         cTTCCTCGCCGTGGAGGCCGCCCTGCAGGCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGccc                                                                                                                           <  1:1298881/150‑1 (MQ=255)

                           tCCTCGCAGTGGAGGCCGCCCTGCAGGCCGGCAGCATGGGCCCGGTGCGCTACTTCGAGTCGGCAATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGc                                                                                                                         >  2:4962671/1‑150 (MQ=255)

                               cGCCGTGGAGGCCGCCCTGCAGGCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGCacct                                                                                                                     <  1:889725/150‑4 (MQ=255)

                                gCCGTGGAGGCCGCCCTGCAGGCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGCacctg                                                                                                                    >  1:3790396/1‑146 (MQ=255)

                                  cGTGGAGGCCGCCCTGCAGGCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGCacctgct                                                                                                                  <  1:7493572/150‑7 (MQ=255)

                                  cGTGGAGGCCGCCCTGCAGGCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGCacctgct                                                                                                                  >  1:5407543/1‑144 (MQ=255)

                                   gTGGAGGCCGCCCTGCAGGCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGCacctgctc                                                                                                                 >  2:1026429/1‑143 (MQ=255)

                                   gTGGAGGCCGCCCTGCAGGCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGCacctgctc                                                                                                                 >  1:10031807/1‑143 (MQ=255)

                                   gTGGAGGCCGCCCTGCAGGCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGCacctgctc                                                                                                                 >  2:6585571/1‑143 (MQ=255)

                                         gccgccCTGCAGGCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACCag                                                                                                          <  1:5012169/150‑2 (MQ=255)

                                         gccgccCTGCAGGCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACCag                                                                                                          <  2:7375711/150‑2 (MQ=255)

                                          ccgccCTGCAGGCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGATTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACCagg                                                                                                         <  2:5612166/150‑3 (MQ=255)

                                             cccTGCAGGCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCAc                                                                                                     >  2:8887564/1‑150 (MQ=255)

                                              ccTGCAGGCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACt                                                                                                    <  2:1351320/150‑1 (MQ=255)

                                               cTGCAGGCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACtg                                                                                                   <  1:2194083/150‑1 (MQ=255)

                                                tGCAGGCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACtgt                                                                                                  <  2:5414070/150‑1 (MQ=255)

                                                 gCAGGCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACtgtg                                                                                                 <  2:1615846/150‑1 (MQ=255)

                                                 gCAGGCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACtgtg                                                                                                 >  1:7792551/1‑150 (MQ=255)

                                                  cAGGCCGGCAGCTTCGGCCCGGTGCGCTACTTCGAGTCGGACATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGc                                                                                                <  2:3188370/150‑1 (MQ=255)

                                                  cAGGCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGc                                                                                                <  2:9779687/150‑1 (MQ=255)

                                                  cAGGCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGc                                                                                                <  1:828733/150‑1 (MQ=255)

                                                   aGGCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGcc                                                                                               >  2:2153926/1‑150 (MQ=255)

                                                    ggCCGGCAGCAACGGCCCGGTGCGCTACTTAGAGTCGGCCATCGAGCGCTTCCGTCCGATGGTCCGCGACCGCTGGCGCGACCACGACCTGCCCGGC‑TCCGGGATGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCt                                                                                              >  2:9304773/1‑150 (MQ=255)

                                                     gCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTg                                                                                             <  2:6386802/150‑1 (MQ=255)

                                                         gcagcaTCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGttcg                                                                                         <  1:7589132/150‑3 (MQ=255)

                                                         gcagcaTCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGttcg                                                                                         >  2:3435788/1‑148 (MQ=255)

                                                           agcaTCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑CGGCACTGTGCCTGTTCGGc                                                                                       <  2:2675228/150‑1 (MQ=255)

                                                             caTCGGCCCGGTGCGCTACTTCGAGTCGGCCCTCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCAt                                                                                     <  1:10611702/150‑1 (MQ=255)

                                                              aTCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATa                                                                                    <  1:8761888/150‑1 (MQ=255)

                                                                cGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATAcc                                                                                  <  2:10574405/150‑1 (MQ=255)

                                                                  gCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGc                                                                                <  1:73021/150‑1 (MQ=255)

                                                                  gCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGc                                                                                <  2:2921024/150‑1 (MQ=255)

                                                                  gCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGc                                                                                >  1:10501249/1‑150 (MQ=255)

                                                                   cccGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCa                                                                               >  1:9448977/1‑150 (MQ=255)

                                                                     cGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGc                                                                             >  1:10190964/1‑150 (MQ=255)

                                                                       gTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGt                                                                           >  2:9552691/1‑150 (MQ=255)

                                                                         gcgcTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTAt                                                                         <  2:4398969/150‑1 (MQ=255)

                                                                          agcTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGCTCCGCGACCGCTCGAGCGAACACGACCACCCCGG‑ATACGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGATCGA‑CAAGTCACTGTGCCAGAACGGCAAACCGCAGCGTATg                                                                        >  1:6391004/2‑150 (MQ=255)

                                                                                 tCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGac                                                                 <  2:10412401/150‑1 (MQ=255)

                                                                                 tCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGac                                                                 >  1:295577/1‑150 (MQ=255)

                                                                                   gAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGacac                                                               <  2:6109495/150‑1 (MQ=255)

                                                                                    aGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACAcc                                                              <  2:5276761/150‑1 (MQ=255)

                                                                                    aGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACAcc                                                              <  1:4934369/150‑1 (MQ=255)

                                                                                      tCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTg                                                            <  1:1892687/150‑1 (MQ=255)

                                                                                       cGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTgc                                                           >  1:5585812/1‑150 (MQ=255)

                                                                                       cGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTgc                                                           >  2:3638835/1‑150 (MQ=255)

                                                                                       cGGCCATCGAGCGCGTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTgc                                                           <  2:5018962/150‑1 (MQ=255)

                                                                                          ccATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGcc                                                        <  2:8010868/150‑1 (MQ=255)

                                                                                             tCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGc                                                     <  1:10283133/150‑1 (MQ=255)

                                                                                             tCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGc                                                     <  1:4232542/150‑1 (MQ=255)

                                                                                              cGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑ACCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCt                                                    >  1:1678750/1‑150 (MQ=255)

                                                                                               gAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTg                                                   >  2:8388328/1‑150 (MQ=255)

                                                                                               gAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTg                                                   >  1:10977258/1‑150 (MQ=255)

                                                                                                aGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTgc                                                  >  1:2018372/1‑150 (MQ=255)

                                                                                                aGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTgc                                                  <  2:10838044/150‑1 (MQ=255)

                                                                                                 gcgcTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTgcg                                                 <  1:5518492/150‑1 (MQ=255)

                                                                                                 gcgcTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTgcg                                                 >  2:6722017/1‑150 (MQ=255)

                                                                                                 gcgcTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTgcg                                                 >  2:4649740/1‑150 (MQ=255)

                                                                                                    cTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGa                                              >  1:6004788/1‑150 (MQ=255)

                                                                                                     ttCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAg                                             >  2:10137547/1‑150 (MQ=255)

                                                                                                          tccgCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGgcgc                                        >  1:4000925/1‑150 (MQ=255)

                                                                                                            cgCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGgcgcgc                                      <  1:1765887/150‑1 (MQ=255)

                                                                                                             gCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCt                                     >  2:1249072/1‑150 (MQ=255)

                                                                                                                ggTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGAc                                  >  2:9055713/1‑150 (MQ=255)

                                                                                                                 gTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGAcc                                 <  1:3514530/150‑1 (MQ=255)

                                                                                                                       gACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCgacgac                           <  1:9795237/150‑1 (MQ=255)

                                                                                                                       gACCGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCgacgac                           <  2:8691056/150‑1 (MQ=255)

                                                                                                                         ccGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTg                         >  1:7027687/1‑150 (MQ=255)

                                                                                                                         ccGCTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTg                         >  2:7596961/1‑150 (MQ=255)

                                                                                                                            cTGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGtt                      >  1:6764054/1‑150 (MQ=255)

                                                                                                                             tGGCGCGAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACAGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTc                     >  1:3572949/1‑150 (MQ=255)

                                                                                                                                cgcgAACACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAg                  <  2:2365964/150‑1 (MQ=255)

                                                                                                                                    aaCACGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTcc              <  1:4714353/150‑1 (MQ=255)

                                                                                                                                       acGACCTGCCCGGC‑TCCGGGCTGTGGTTCGACCACGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGTCACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGc           >  1:5298732/1‑150 (MQ=255)

                                                                                                                                        cGACCTGCCCGGC‑TCCGGGCGGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑CGGCACGGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCt          <  2:7826572/150‑1 (MQ=255)

                                                                                                                                          aCCTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCTCg        >  2:7236808/1‑150 (MQ=255)

                                                                                                                                           ccTGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCTCGa       >  1:5689293/1‑150 (MQ=255)

                                                                                                                                             tGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCTCGACt     <  2:4051591/150‑1 (MQ=255)

                                                                                                                                             tGCCCGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCTCGACt     <  1:6632512/150‑1 (MQ=255)

                                                                                                                                               cccGGC‑TCCGGGCTGTGGTTCGACCTCGGCCCGC‑ACCTGCTCGACC‑AGGCACTGTGCCTGTTCGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCTCGACTAt   <  1:9232329/150‑1 (MQ=255)

TCCAGAACCGGCGCTTCGACAGCGACTTCCTCGCCGTGGAGGCCGCCCTGCAGGCCGGCAGCATCGGCCCGGTGCGCTACTTCGAGTCGGCCATCGAGCGCTTCCGTCCGCAGGTCCGCGACCGCTGGCGCGAACACGACCTGCCCGGCTTCCGGGCTGTGGTTTGACCTTGGCCCGCAACCTGGTTGACCAAGGCACTGTGCCTGTTTGGCATACCGCAGCGTATGCACGGACACCTGCGCCGGCTGCGCGAGGGCGCGCTGACCGACGACTGGTTCGAGGTCCTGCTCGACTATC  >  NC_008463/6069914‑6070210

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`