Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 6,102,509 (G)5→4 intergenic (+35/+18) yrfG → / ← lysM GMP/IMP nucleotidase/peptidoglycan‑binding protein LysM

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084636,102,5050G.100.0% 3106.2 / NA 625intergenic (+31/+22)yrfG/lysMGMP/IMP nucleotidase/peptidoglycan‑binding protein LysM
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (249/376);  total (249/376)

Only 100 of 671 total aligned reads displayed.

CCTATGGTGTCGCCCACCTGTTGGCGGTACGCCACCCGGACAGCCGTGGCCAGGTCCGGGACACCGAGGAGTTCGCTGCCCAGGAGGACTACCGGCGGCTGCTCGAAGGACTGCCGTAGCCGCGGAAACGAAAACCCCGCCACGCGGGCGGGGGTTGGCGAAACGCCGGGATCACTCGGGAATGCGCAGGACCTGGCCGGGGTAGATCTTGTCCGGATGGCTGAGCATCGGCTTGTTGGCCTCGAAGATCTTCATGTAGGCGTTGGCATTGCCGTATTCGGCCTTGGCGATGGCG  >  NC_008463/6102356‑6102650
                                                                                                                                                     |                                                                                                                                                 
   aTGGTGTCGCCCACCTGTTGGCGGTACGCCACCCGGACAGCCGTGGCCAGGTCCGGGACACCGAGGAGTTCGCTGCCCAGGAGGACTACCGGCGGCTGCTCGAAGGACTGCCGTAGCCGCGGAAACGAAAACCCCGCCACGCGGGCgggg                                                                                                                                                >  1:3504810/1‑150 (MQ=255)
     ggTGTCGCCCACCTGTTGGCGGTACGCCACCCGGACAGCCGTGGCCAGGTCCGGGACACCGAGGAGTTCGCTGCCCAGGAGGACTACCGGCGGCTGCTCGAAGGACTGCCGTAGCCGCGGAAACGAAAACCCCGCCACGCGGGCggggtt                                                                                                                                              <  1:8994621/150‑3 (MQ=255)
     ggTGTCGCCCACCTGTTGGCGGTACGCCACCCGGACAGCCGTGGCCAGGTCCGGGACACCGAGGAGTTCGCTGCCCAGGAGGACTACCGGCGGCTGCTCGAAGGACTGCCGTAGCCGCGGAAACGAAAACCCCGCCACGCGGGCggggtt                                                                                                                                              <  1:1126668/150‑3 (MQ=255)
     ggTGTCGCCCACCTGTTGGCGGTACGCCACCCGGACAGCCGTGGCCAGGTCCGGGACACCGAGGAGTTCGCTGCCCAGGAGGACTACCGGCGGCTGCTCGAAGGACTGCCGTAGCCGCGGAAACGAAAACCCCGCCACGCGGGCggggtt                                                                                                                                              <  2:3545916/150‑3 (MQ=255)
       tgtCGCCCACCTGTTGGCGGTACGCCACCCGGACAGCCGTGGCCAGGTCCGGGACACCGAGGAGTTCGCTGCCCAGGAGGACTACCGGCGGCTGCTCGAAGGACTGCCGTAGCCGCGGAAACGAAAACCCCGCCACGCGGGCggggttgg                                                                                                                                            <  1:7615408/150‑5 (MQ=255)
       tgtCGCCCACCTGTTGGCGGTACGCCACCCGGACAGCCGTGGCCAGGTCCGGGACACCGAGGAGTTCGCTGCCCAGGAGGACTACCGGCGGCTGCTCGAAGGACTGCCGTAGCCGCGGAAACGAAAACCCCGCCACGCGGGCggggttgg                                                                                                                                            >  2:10898296/1‑146 (MQ=255)
         tCGCCCACCTGTTGGCGGTACGCCACCCGGACAGCCGTGGCCAGGTCCGGGACACCGAGGAGTTCGCTGCCCAGGAGGACTACCGGCGGCTGCTCGAAGGACTGCCGTAGCCGCGGAAACGAAAACCCCGCCACGCGGGC‑GGGGTTGGCg                                                                                                                                         <  1:7436118/150‑1 (MQ=255)
          cGCCCACCTGTTGGCGGTACGCCACCCGGACAGCCGTGGCCAGGTCCGGGACACCGAGGAGTTCGCTGCCCAGGAGGACTACCGGCGGCTGCTCGAAGGACTGCCGTAGCCGCGGAAACGAAAACCCCGCCACGCGGGC‑GGGGTTGGCGa                                                                                                                                        <  2:5687696/150‑1 (MQ=255)
           gCCCACCTGTTGGCGGTACGCCACCCGGACAGCCGTGGCCAGGTCCTGGACACCGAGGAGTTCGCTGCCCAGGAGGACTACCGGCGGCTGCTCGAAGGACTGCCGTAGCCGCGGAAACGAAAACCCCGCCACGCGGGC‑GGGGTTGGCGaa                                                                                                                                       <  2:1468559/150‑1 (MQ=255)
           gCCCACCTGTTGGCGGTACGCCACCCGGACAGCCGTGGCCAGGTCCGGGACACCGAGGAGTTCGCTGCCCAGGAGGACTACCGGCGGCTGCTCGAAGGACTGCCGTAGCCGCGGAAACGAAAACCCCGCCACGCGGGC‑GGGGTTGGCGaa                                                                                                                                       >  1:9599410/1‑150 (MQ=255)
             ccACCTGTTGGCGGTACGCCACCCGGACAGCCGTGGCCAGGTCCGGGACACCGAGGAGTTCGCTGCCCAGGAGGACTACCGGCGGCTGCTCGAAGGACTGCCGTAGCCGCGGAAACGAAAACCCCGCCACGCGGGC‑GGGGTTGGCGAAAc                                                                                                                                     >  1:30791/1‑150 (MQ=255)
             ccACCTGTTGGCGGTACGCCACCCGGACAGCCGTGGCCAGGTCCGGGACACCGAGGAGTTCGCTGCCCAGGAGGACTACCGGCGGCTGCTCGAAGGACTGCCGTAGCCGCGGAAACGAAAACCCCGCCACGCGGGC‑GGGGTTGGCGAAAc                                                                                                                                     >  1:4365217/1‑150 (MQ=255)
             ccACCTGTTGGCGGTACGCCACCCGGACAGCCGTGGCCAGGTCCGGGACACCGAGGAGTTCGCTGCCCAGGAGGACTACCGGCGGCTGCTCGAAGGACTGCCGTAGCCGCGGAAACGAAAACCCCGCCACGCGGGC‑GGGGTTGGCGAAAc                                                                                                                                     >  2:7449963/1‑150 (MQ=255)
               aCCTGTTGGCGGTACGCCACCCGGACAGCCGTGGCCAGGTCCGGGACACCGAGGAGTTCGCTGCCCAGGAGGACTACCGGCGGCTGCTCGAAGGACTGCCGTAGCCGCGGAAACGAAAACCCCGCCACGCGGGC‑GGGGTTGGCGAAACGc                                                                                                                                   <  2:2784962/150‑1 (MQ=255)
               aCCTGTTGGCGGTACGCCACCCGGACAGCCGTGGCCAGGTCCGGGACACCGAGGAGTTCGCTGCCCAGGAGGACTACCGGCGGCTGCTCGAAGGACTGCCGTAGCCGCGGAAACGAAAACCCCGCCACGCGGGC‑GGGGTTGGCGAAACGc                                                                                                                                   <  2:3933172/150‑1 (MQ=255)
                 cTGTTGGCGGTACGCCACCCGGACAGCCGTGGCCAGGTCCGGGACACCGAGGAGTTCGCTGCCCAGGAGGACTACCGGCGGCTGCTCGAAGGACTGCCGTAGCCGCGGAAACGAAAACCCCGCCACGCGGGC‑GGGGTTGGCGAAACGCCg                                                                                                                                 >  1:4889604/1‑150 (MQ=255)
                  tGTTGGCGGTACGCCACCCGGACAGCCGTGGCCAGGTCCGGGACACCGAGGAGTTCGCTGCCCAGGAGGACTACCGGCGGCTGCTCGAAGGACTGCCGTAGCCGCGGAAACGAAAACCCCGCCACGCGGGC‑GGGGTTGGCGAAACGCCgg                                                                                                                                <  2:6304616/150‑1 (MQ=255)
                  tGTTGGCGGTACGCCACCCGGACAGCCGTGGCCAGGTCCGGGACACCGAGGAGTTCGCTGCCCAGGAGGACTACCGGCGGCTGCTCGAAGGACTGCCGTAGCCGCGGAAACGAAAACCCCGCCACGCGGGC‑GGGGTTGGCGAAACGCCgg                                                                                                                                >  2:10628323/1‑150 (MQ=255)
                    ttGGCGGTACGCCACCCGGACAGCCGTGGCCAGGTCCGGGACACCGAGGAGTTCGCTGCCCAGGAGGACTACCGGCGGCTGCTCGAAGGACTGCCGTAGCCGCGGAAACGAAAACCCCGCCACGCGGGC‑GGGGTTGGCGAAACGCCGGGa                                                                                                                              >  1:7758450/1‑150 (MQ=255)
                       gCGGTACGCCACCCGGACAGCCGTGGCCAGGTCCGGGACACCGAGGAGTTCGCTGCCCAGGAGGACTACCGGCGGCTGCTCGAAGGACTGCCGTAGCCGCGGAAACGAAAACCCCGCCACGCGGGC‑GGGGTTGGCGAAACGCCGGGATcn                                                                                                                           <  2:2838589/150‑2 (MQ=255)
                        cGGTACGCCACCCGGACAGCCGTGGCCAGGTCCGGGACACCGAGGAGTTCGCTGCCCAGGAGGACTACCGGCGGCTGCTCGAAGGACTGCCGTAGCCGCGGAAACGAAAACCCCGCCACGCGGGC‑GGGGTTGGCGAAACGCCGGGATCAc                                                                                                                          <  1:10945601/150‑1 (MQ=255)
                         ggTACGCCACCCGGACAGCCGTGGCCAGGTCCGGGACACCGAGGAGTTCGCTGCCCAGGAGGACTACCGGCGGCTGCTCGAAGGACTGCCGTAGCCGCGGAAACGAAAACCCCGCCACGCGGGC‑GGGGTTGGCGAAACGCCGGGATCACt                                                                                                                         <  2:6915055/150‑1 (MQ=255)
                            aCGCCACCCGGACAGCCGTGGCCAGGTCCGGGACACCGAGGAGTTCGCTGCCCAGGAGGACTACCGGCGGCTGCTCGAAGGACTGCCGTAGCCGCGGAAACGAAAACCCCGCCACGCGGGC‑GGGGTTGGCGAAACGCCGGGATCACTCgg                                                                                                                      <  2:11046956/150‑1 (MQ=255)
                            aCGCCACCCGGACAGCCGTGGCCAGGTCCGGGACACCGAGGAGTTCGCTGCCCAGGAGGACTACCGGCGGCTGCTCGAAGGACTGCCGTAGCCGCGGAAACGAAAACCCCGCCACGCGGGC‑GGGGTTGGCGAAACGCCGGGATCACTCgg                                                                                                                      >  2:7687193/1‑150 (MQ=255)
                               ccACCCGGACAGCCGTGGCCAGGTCCGGGACACCGAGGAGTTCGCTGCCCAGGAGGACTACCGGCGGCTGCTCGAAGGACTGCCGTAGCCGCGGAAACGAAAACCCCGCCACGCGGGC‑GGGGTTGGCGAAACGCCGGGATCACTCGGGaa                                                                                                                   <  1:8323672/150‑1 (MQ=255)
                                cACCCGGACAGCCGTGGCCAGGTCCGGGACACCGAGGAGTTCGCTGCCCAGGAGGACTACCGGCGGCTGCTCGAAGGACTGCCGTAGCCGCGGAAACGAAAACCCCGCCACGCGGGC‑GGGGTTGGCGAAACGCCGGGATCACTCGGGAAt                                                                                                                  <  2:8625327/150‑1 (MQ=255)
                                 aCCCGGACAGCCGTGGCCAGGTCCGGGACACCGAGGAGTTCGCTGCCCAGGAGGACTACCGGCGGCTGCTCGAAGGACTGCCGTAGCCGCGGAAACGAAAACCCCGCCACGCGGGC‑GGGGTTGGCGAAACGCCGGGATCACTCGGGAATg                                                                                                                 <  1:5543681/150‑1 (MQ=255)
                                  cccGGACAGCCGTGGCCAGGTCCGGGACACCGAGGAGTTCGCTGCCCAGGAGGACTACCGGCGGCTGCTCGAAGGACTGCCGTAGCCGCGGAAACGAAAACCCCGCCACGCGGGC‑GGGGTTGGCGAACCGCCGGGATCACTCGGGACTgc                                                                                                                >  2:9577177/1‑150 (MQ=255)
                                      gACAGCCGTGGCCAGGTCCGGGACACCGAGGAGTTCGCTGCCCAGGAGGACTACCGGCGGCTGCTCGAAGGACTGCCGTAGCCGCGGAAACGAAAACCCCGCCACGCGGGC‑GGGGTTGGCGAAACGCCGGGATCACTCGGGAATGCGCAg                                                                                                            <  1:8642651/150‑1 (MQ=255)
                                      gACAGCCGTGGCCAGGTCCGGGACACCGAGGAGTTCGCTGCCCAGGAGGACTACCGGCGGCTGCTCGAAGGACTGCCGTAGCCGCGGAAACGAAAACCCCGCCACGCGGGC‑GGGGTTGGCGAAACGCCGGGATCACTCGGGAATGCGCAg                                                                                                            <  1:1453266/150‑1 (MQ=255)
                                       aCAGCCGTGGCCAGGTCCGGGACACCGAGGAGTTCGCTGCCCAGGAGGACTACCGGCGGCTGCTCGAAGGACTGCCGTAGCCGCGGAAACGAAAACCCCGCCACGCGGGC‑GGGGTTGGCGAAACGCCGGGATCACTCGGGAATGCGCAgg                                                                                                           >  2:7966958/1‑150 (MQ=255)
                                           ccGTGGCCAGGTCCGGGACACCGAGGAGTTCGCTGCCCAGGAGGACTACCGGCGGCTGCTCGAAGGACTGCCGTAGCCGCGGAAACGAAAACCCCGCCACGCGGGC‑GGGGTTGGCGAAACGCCGGGATCACTCGGGAATGCGCAGGACCt                                                                                                       >  1:3739458/1‑150 (MQ=255)
                                             gTGGCCAGGTCCGGGACACCGAGGAGTTCGCTGCCCAGGAGGACTACCGGCGGCTGCTCGAAGGACTGCCGTAGCCGCGGAAACGAAAACCCCGCCACGCGGGC‑GGGGTTGGCGAAACGCCGGGATCACTCGGGAATGCGCAGGACCTgg                                                                                                     >  1:7286344/1‑150 (MQ=255)
                                                gCCAGGTCCGGGACACCGAGGAGTTCGCTGCCCAGGAGGACTACCGGCGGCTGCTCGAAGGACTGCCTTAGCCGCGGAAACGAAAACCCCGCCACGCGGGC‑GGGGTTGGCGAAACGCCGGGATCACTCGGGACTGCGCAGGACCTGGCCg                                                                                                  >  1:1102821/1‑150 (MQ=255)
                                                gCCAGGTCCGGGACACCGAGGAGTTCGCTGCCCAGGAGGACTACCGGCGGCTGCTCGAAGGACTGCCGTAGCCGCGGAAACGAAAACCCCGCCACGCGGGC‑GGGGTTGGCGAAACGCCGGGATCACTCGGGACTGCGCAGGACCTGGCCg                                                                                                  >  2:8788131/1‑150 (MQ=255)
                                                    ggTCCGGGACACCGAGGAGTTCGCTGCCCAGGAGGACTACCGGCGGCTGCTCGAAGGACTGCCGTAGCCGCGGAAACGAAAACCCCGCCACGCGGGC‑GGGGTTGGCGAAACGCCGGGATCACTCGGGAATGCGCAGGACCTGGCCGGGGt                                                                                              >  1:6838845/1‑150 (MQ=255)
                                                    ggTCCGGGACACCGAGGAGTTCGCTGCCCAGGAGGACTACCGGCGGCTGCTCGAAGGACTGCCGTAGCCGCGGAAACGAAAACCCCGCCACGCGGGC‑GGGGTTGGCGAAACGCCGGGATCACTCGGGAATGCGCAGGACCTGGCCGGGGt                                                                                              >  1:1958593/1‑150 (MQ=255)
                                                     gTCCGGGACACCGAGGAGTTCGCTGCCCAGGAGGACTACCGGCGGCTGCTCGAAGGACTGCCGTAGCCGCGGAAACGAAAACCCCGCCACGCGGGC‑GGGGTTGGCGAAACGCCGGGATCACTCGGGAATGCGCAGGACCTGGCCGGGGTa                                                                                             <  1:6330168/150‑1 (MQ=255)
                                                     gTCCGGGACACCGAGGAGTTCGCTGCCCAGGAGGACTACCGGCGGCTGCTCGAAGGACTGCCGTAGCCGCGGAAACGAAAACCCCGCCACGCGGGC‑GGGGTTGGCGAAACGCCGGGATCACTCGGGAATGCGCAGGACCTGGCCGGGGTa                                                                                             <  1:3286397/150‑1 (MQ=255)
                                                        cGGGACACCGAGGAGTTCGCTGCCCAGGAGGACTACCGGCGGCTGCTCGAAGGACTGCCGTAGCCGCGGAAACGAAAACCCCGCCACGCGGGC‑GGGGTTGGCGAAACGCCGGGATCACTCGGGAATGCGCAGGACCTGGCCGGGGTAGAt                                                                                          <  2:4866157/150‑1 (MQ=255)
                                                           gACACCGAGGAGTTCGCTGCCCAGGAGGACTACCGGCGGCTGCTCGAAGGACTGCCGTAGCCGCGGAAACGAAAACCCCGCCACGCGGGC‑GGGGTTGGCGAAACGCCGGGATCACTCGGGAATGCGCAGGACCTGGCCGGGGTAGATCtt                                                                                       <  2:4501714/150‑1 (MQ=255)
                                                            acacCGAGGAGTTCGCTGCCCAGGAGGACTACCGGCGGCTGCTCGAAGGACTGCCGTAGCCGCGGAAACGAAAACCCCGCCACGCGGGC‑GGGGTTGGCGAAACGCCGGGATCACTCGGGAATGCGCAGGACCTGGCCGGGGTAGATCTTg                                                                                      <  2:930666/150‑1 (MQ=255)
                                                             cacCGAGGAGTTCGCTGCCCAGGAGGACTACCGGCGGCTGCTCGAAGGACTGCCGTAGCCGCGGAAACGAAACCCCCGCCACGCGGGC‑GGGGTTGGCGAAACGCCGGGATCACTCGGGAATGCGCAGGACCTGGCCGGGGTAGATCTTGt                                                                                     <  2:2324221/150‑1 (MQ=255)
                                                              acCGAGGAGTTCGCTGCCCAGGAGGACTACCGGCGGCTGCTCGAAGGACTGCCGTAGCCGCGGAAACGAAAACCCCGCCACGCGGGC‑GGGGTTGGCGAAACGCCGGGATCACTCGGGAATGCGCAGGACCTGGCCGGGGTAGATCTTGTc                                                                                    <  1:8458073/150‑1 (MQ=255)
                                                               ccGAGGAGTTCGCTGCCCAGGAGGACTACCGGCGGCTGCTCGAAGGACTGCCGTAGCCGCGGAAACGAAAACCCCGCCACGCGGGC‑GGGGTTGGCGAAACGCCGGGATCACTCGGGAATGCGCAGGACCTGGCCGGGGTAGATCTTGTcc                                                                                   <  1:5889262/150‑1 (MQ=255)
                                                                 gaggagTTCGCTGCCCAGGAGGACTACCGGCGGCTGCTCGAAGGACTGCCGTAGCCGCGGAAACGAAAACCCCGCCACGCGGGC‑GGGGTTGGCGAAACGCCGGGATCACTCGGGAATGCGCAGGACCTGGCCGGGGTAGATCTTGTCCgg                                                                                 >  1:2441112/1‑150 (MQ=255)
                                                                    gagTTCGCTGCCCAGGAGGACTACCGGCGGCTGCTCGAAGGACTGCCGTAGCCGCGGAAACGAAAACCCCGCCACGCGGGC‑GGGGTTGGCGAAACGCCGGGATCACTCGGGAATGCGCAGGACCTGGCCGGGGTAGATCTTGTCCGGATg                                                                              >  1:4249778/1‑150 (MQ=255)
                                                                     agTTCGCTGCCCAGGAGGACTACCGGCGGCTGCTCGAAGGACTGCCGTAGCCGCGGAAACGAAAACCCCGCCACGCGGGC‑GGGGTTTGCGAAACGCCGGGATCACTCGGGAATGCGCAGGACCTGGCCGGGGTAGATCTTGTCCGGATgg                                                                             >  2:4050175/1‑150 (MQ=255)
                                                                      gTTCGCTGCCCAGGAGGACTACCGGCGGCTGCTCGAAGGACTGCCGTAGCCGCGGAAACGAAAACCCCGCCACGCGGGC‑GGGGTTGGCGAAACGCCGGGATCACTCGGGAATGCGCAGGACCTGGCCGGGGTAGATCTTGTCCGGATGGc                                                                            <  2:325817/150‑1 (MQ=255)
                                                                       ttCGCTGCCCAGGAGGACTACCGGCGGCTGCTCGAAGGACTGCCGTAGCCGCGGAAACGAAAACCCCGCCACGCGGGC‑GGGGTTGGCGAAACGCCGGGATCACTCGGGAATGCGCAGGACCTGGCCGGGGTAGATCTTGTCCGGATGGCt                                                                           <  1:10025470/150‑1 (MQ=255)
                                                                        tCGCTGCCCAGGAGGACTACCGGCGGCTGCTCGAAGGACTGCCGTAGCCGCGGAAACGAAAACCCCGCCACGCGGGC‑GGGGTTGGCGAAACGCCGGGATCACTCGGGAATGCGCAGGACCTGGCCGGGGTAGATCTTGTCCGGATGGCTg                                                                          >  1:8837150/1‑150 (MQ=255)
                                                                         cGCTGCCCAGGAGGACTACCGGCGGCTGCTCGAAGGACTGCCGTAGCCGCGGAAACGAAAACCCCGCCACGCGGGC‑GGGGTTGGCGAAACGCCGGGATCACTCGGGAATGCGCAGGACCTGGCCGGGGTAGATCTTGTCCGGATGGCTGa                                                                         <  2:10518202/150‑1 (MQ=255)
                                                                            tGCCCAGGAGGACTACCGGCGGCTGCTCGAAGGACTGCCGTAGCCGCGGAAACGAAAACCCCGCCACGCGGGC‑GGGGTTGGCGAAACGCCGGGATCACTCGGGAATGCGCAGGACCTGGCCGGGGTAGATCTTGTCCGGATGGCTGAGCa                                                                      <  2:9472896/150‑1 (MQ=255)
                                                                             gCCCAGGAGGACTACCGGCGGCTGCTCGAAGGACTGCCGTAGCCGCGGAAACGAAAACCCCGCCACGCGGGC‑GGGGTTGGCGAAACGCCGGGATCACTCGGGAATGCGCAGGACCTGGCCGGGGTAGATCTTGTCCGGATGGCTGAGCAt                                                                     <  2:2935704/150‑1 (MQ=255)
                                                                              cccAGGAGGACTACCGGCGGCTGCTCGAAGGACTGCCGTAGCCGCGGAAACGAAAACCCCGCCACGCGGGC‑GGGGTTGGCGAAACGCCGGGATCACTCGGGAATGCGCAGGACCTGGCCGGGGTAGATCTTGTCCGGATGGCTGAGCATc                                                                    >  2:10112717/1‑150 (MQ=255)
                                                                              cccAGGAGGACTACCGGCGGCTGCTCGAAGGACTGCCGTAGCCGCGGAAACGAAAACCCCGCCACGCGGGC‑GGGGTTGGCGAAACGCCGGGATCACTCGGGAATGCGCAGGACCTGGCCGGGGTAGATCTTGTCCGGATGGCTGAGCATc                                                                    <  1:2224867/150‑1 (MQ=255)
                                                                                    aggaCTACCGGCGGCTGCTCGAAGGACTGCCGTAGCCGCGGAAACGAAAACCCCGCCACGCGGGC‑GGGGTTGGCGAAACGCCGGGATCACTCGGGAATGCGCAGGACCTGGCCGGGGTAGATCTTGTCCGGATGGCTGAGCATCGGCttg                                                              >  2:3154536/1‑150 (MQ=255)
                                                                                     ggaCTACCGGCGGCTGCTCGAAGGACTGCCGTAGCCGCGGAAACGAAAACCCCGCCACGCGGGC‑GGGGTTGGCGAAACGCCGGGATCACTCGGGAATGCGCAGGACCTGGCCGGGGTAGATCTTGTCCGGATGGCTGAGCAACGGCttgt                                                             <  1:5287644/150‑1 (MQ=255)
                                                                                        cTACCGGCGGCTGCTCGAAGGACTGCCGTAGCCGCGGAAACGAAAACCCCGCCACGCGGGC‑GGGGTTGGCGAAACGCCGGGATCACTCGGGAATGCGCAGGACCTGGCCGGGGTAGATCTTGTCCGGATGGCTGAGCATCGGCTTGTTgg                                                          <  2:1886218/150‑1 (MQ=255)
                                                                                         tACCGGCGGCTGCTCGAAGGACTGCCGTAGCCGCGGAAACGAAAACCCCGCCACGCGGGC‑GGGGTTGGCGAAACGCCGGGATCACTCGGGAATGCGCAGGACCTGGCCGGGGTAGATCTTGTCCGGATGGCTGAGCATCGGCTTGTTGGc                                                         >  1:9410339/1‑150 (MQ=255)
                                                                                          aCCGGCGGCTGCTCGAAGGACTGCCGTAGCCGCGGAAACGAAAACCCCGCCACGCGGGC‑GGGGTTGGCGAAACGCCGGGATCACTCGGGAATGCGCAGGACCTGGCCGGGGTAGATCTTGTCCGGATGGCTGAGCATCGGCTTGTTGGcc                                                        >  1:5103121/1‑150 (MQ=255)
                                                                                           ccGGCGGCTGCTCGAAGGACTGCCGTAGCCGCGGAAACGAAAACCCCGCCACGCGGGC‑GGGGTTGGCGAAACGCCGGGATCACTCGGGAATGCGCAGGACCTGGCCGGGGTAGATCTTGTCCGGATGGCTGAGCATCGGCTTGTTGGCCt                                                       <  2:167460/150‑1 (MQ=255)
                                                                                             ggcggcTGCTCGAAGGACTGCCGTAGCCGCGGAAACGAAAACCCCGCCACGCGGGC‑GGGGTTGGCGAAACGCCGGGATCACTCGGGAATGCGCAGGACCTGGCCGGGGTAGATCTTGTCCGGATGGCTGAGCATCGGCTTGTTGGCCTCg                                                     <  1:6508097/150‑1 (MQ=255)
                                                                                                ggcTGCTCGAAGGACTGCCGTAGCCGCGGAAACGAAAACCCCGCCACGCGGGC‑GGGGTTGGCGAAACGCCGGGATCACTCGGGAATGCGCAGGACCTGGCCGGGGTAGATCTTGTCCGGATGGCTGAGCATCGGCTTGTTGGCCTCGAAg                                                  <  2:6516996/150‑1 (MQ=255)
                                                                                                 gctgctCGAAGGTCTGCCGTAGCCGCGGAAACGAAAACCCCGCCACGCGGGC‑GGGGTTGGCGAAACGCCGGGATCACTCGGGAATGCGCAGGACCTGGCCGGGGTAGATCTTGTCCGGATGGCTGAGCATCGGCTTGTTGGCCTCGAAGa                                                 <  2:2089216/150‑1 (MQ=255)
                                                                                                 gctgctCGAAGGACTGCCGTAGCCGCGGAAACGAAAACCCCGCCACGCGGGC‑GGGGTTGGCGAAACGCCGGGATCACTCGGGAATGCGCAGGACCTGGCCGGGGTAGATCTTGTCCGGATGGCTGAGCATCGGCTTGTTGGCCTCGAAGa                                                 <  2:2551038/150‑1 (MQ=255)
                                                                                                   tgctCGAAGGACTGCCGTAGCCGCGGAAACGAAAACCCCGCCACGCGGGC‑GGGGTTGGCGAAACGCCGGGATCACTCGGGAATGCGCAGGACCTGGCCGGGGTAGATCTTGTCCGGATGGCTGAGCATCGGCTTGTTGGCCTCGAAGATc                                               >  2:7495160/1‑150 (MQ=255)
                                                                                                     ctCGAAGGACTGCCGTAGCCGCGGAAACGAAAACCCCGCCACGCGGGC‑GGGGTTGGCGAAACGCCGGGATCACTCGGGAATGCGCAGGACCTGGCCGGGGTAGATCTTGTCCGGATGGCTGAGCATCGGCTTGTTGGCCTCGAAGATCtt                                             <  2:10303902/150‑1 (MQ=255)
                                                                                                         aaGGACTGCCGTAGCCGCGGAAACGAAAACCCCGCCACGCGGGC‑GGGGTTGGCGAAACGCCGGGATCACTCGGGAATGCGCAGGACCTGGCCGGGGTAGATCTTGTCCGGATGGCTGAGCATCGGCGTGTTGGCCTCGAAGATCTTCATg                                         >  1:2713709/1‑150 (MQ=255)
                                                                                                          aGGACTGCCGTAGCCGCGGAAACGAAAACCCCGCCACGCGGGC‑GGGGTTGGCGAAACGCCGGGATCACTCGGGAATGCGCAGGACCTGGCCGGGGTAGATCTTGTCCGGATGGCTGAGCATCGGCTTGTTGGCCTCGAAGATCTTCATGt                                        <  2:8947445/150‑1 (MQ=255)
                                                                                                             aCTGCCGTAGCCGCGGAAACGAAAACCCCGCCACGCGGGC‑GGGGTTGGCGAAACGCCGGGATCACTCGGGAATGCGCAGGACCTGGCCGGGGTAGATCTTGTCCGGATGGCTGAGCATCGGCTTGTTGGCCTCGAAGATCTTCATGTAgg                                     <  2:5082337/150‑1 (MQ=255)
                                                                                                              cTGCCGTAGCCGCGGAAACGAAAACCCCGCCACGCGGGC‑GGGGTTGGCGAAACGCCGGGATCACTCGGGACTGCGCAGGACCTGGCCGGGGTAGATCTTGTCCGGATGGCTGAGCATCGGGGTGGTGGCCTCGAAGAGCTTCTTGGAGGc                                    >  2:2487818/1‑150 (MQ=255)
                                                                                                                gCCGTAGCCGCGGAAACGAAAACCCCGCCACGCGGGC‑GGGGTTGGCGAAACGCCGGGATCACTCGGGAATGCGCAGGACCTGGCCGGGGTAGATCTTGTCCGGATGGCTGAGCATCGGCTTGTTGGCCTCGAAGATCTTCATGTAGGCGt                                  >  2:5191044/1‑150 (MQ=255)
                                                                                                                gCCGTAGCCGCGGAAACGAAAACCCCGCCACGCGGGC‑GGGGTTGGCGAAACGCCGGGATCACTCGGGAATGCGCAGGACCTGGCCGGGGTAGATCTTGTCCGGATGGCTGAGCATCGGCTTGTTGGCCTCGAAGATCTTCAGGTAGGCGt                                  <  2:4185910/150‑1 (MQ=255)
                                                                                                                     aGCCGCGGAAACGAAAACCCCGCCACGCGGGC‑GGGGTTGGCGAAACGCCGGGATCACTCGGGAATGCGCAGGACCTGGCCGGGGTAGATCTTGTCCGGATGGCTGAGCATCGGCTTGTTGGCCACGAAGATCTACATGTAGGCGTTGGCa                             >  1:4672549/1‑150 (MQ=255)
                                                                                                                      gCCGCGGAAACGAAATCCCCGCCACGCGGGC‑GGGGTTGGCGAAACGCCGGGATCACTCGGGAATGCGCAGGACCTGGCCGGGGTAGATCTTGTCCGGATGGCTGAGCATCGGCTTGTTGGCCTCGATGATCTTCATGTCGGCGTTGGCAt                            >  2:9883460/1‑150 (MQ=255)
                                                                                                                      gCCGCGGAAACGAAAACCCCGCCACGCGGGC‑GGGGTTGGCGAAACGCCGGGATCACTCGGGAATGCGCAGGACCTGGCCGGGGTAGATCTTGTCCGGATGGCTGAGCATCGGCTTGTTGGCCTCGAAGATCTTCATGTAGGCGTTGGCAt                            >  2:3463485/1‑150 (MQ=255)
                                                                                                                       ccGCGGAAACGAAAACCCCGCCACGCGGGC‑GGGGTTGGCGAAACGCCGGGATCACTCGGGAATGCGCAGGACCTGGCCGGGGTAGATCTTGTCCGGATGGCTGAGCATCGGCTTGTTGGCCTCGAAGATCTTCATGTAGGCGTTGGCAtt                           <  1:4020922/150‑1 (MQ=255)
                                                                                                                       ccGCGGAAACGAAAACCCCGCCACGCGGGC‑GGGGTTGGCGAAACGCCGGGATCACTCGGGAATGCGCAGGACCTGGCCGGGGTAGATCTTGTCCGGATGGCTGAGCATCGGCTTGTTGGCCTCGAAGATCTTCATGTAGGCGTTGGCAtt                           >  2:7142421/1‑150 (MQ=255)
                                                                                                                        cgcgGAAACGAAACCCCCGCCACGCGGGC‑GGGGTTGGCGAAACGCCGGGATCACTCGGGAATGCGCAGGACCTGGCCGGGGTAGATCTTGTCCGGATGGCTGAGCATCGGCTTGTTGGCCTCGAAGATCTTCATGTAGGCGTTGGCATTg                          <  2:7917944/150‑1 (MQ=255)
                                                                                                                        cgcgGAAACGAAAACCCCGCCACGCGGGC‑GGGGTTGGCGAAACGCCGGGATCACTCGGGAATGCGCAGGACCTGGCCGGGGTAGATCTTGTCCGGATGGCTGAGCATCGGCTTGTTGGCCTCGAAGATCTTCATGTAGGCGTTGGCATTg                          <  1:10757482/150‑1 (MQ=255)
                                                                                                                         gcgGAAACGAAAACCCCGCCACGCGGGC‑GGGGTTGGCGAAACGCCGGGATCACTCGGGACTGCGCAGGACCTGGCCGGGGTAGATCTTGTCCGGATGGCTGAGCATCGGCTTGTTGGCCTCGAAGATCTTCATGTAGGGGTTGGCATTGc                         >  2:6732045/1‑150 (MQ=255)
                                                                                                                         gcgGAAACGAAAACCCCGCCACGCGGGC‑GGGGTTGGCGAAACGCCGGGATCACTCGGGAATGCGCAGGACCTGGCCGGGGTAGATCTTGTCCGGATGGCTGAGCATCGGCTTGTTGGCCTCGAAGATCTTCATGTAGGCGTTGGCATTGc                         >  1:1768363/1‑150 (MQ=255)
                                                                                                                         gcgGAAACGAAAACCCCGCCACGCGGGC‑GGGGTTGGCGAAACGCCGGGATCACTCGGGAATGCGCAGGACCTGGCCGGGGTAGATCTTGTCCGGATGGCTGAGCATCGGCTTGTTGGCCTCGAAGATCTTCATGTAGGCGTTGGCATTGc                         >  1:2319873/1‑150 (MQ=255)
                                                                                                                            gAAACGAAAACCCCGCCACGCGGGC‑GGGGTTGGCGAAACGCCGGGATCACTCGGGAATGCGCAGGACCTGGCCGGGGTAGATCTTGTCCGGATGGCTGAGCATCGGCTTGTTGGCCTCGAAGATCTTCATGTAGGCGTTGGCATTGCCGt                      <  1:9693121/150‑1 (MQ=255)
                                                                                                                             aaaCGAAAACCCCGCCACGCGGGC‑GGGGTTGGCGAAACGCCGGGATCACTCGGGAATGCGCAGGACCTGGCCGGGGTAGATCTTGTCCGGATGGCTGAGCATCGGCTTGTTGGCCTCGAAGATCTTCATGTAGGCGTTGGCATTGCCGTa                     <  1:1619291/150‑1 (MQ=255)
                                                                                                                             aaaCGAAAACCCCGCCACGCGGGC‑GGGGTTGGCGAAACGCCGGGATCACTCGGGAATGCGCAGGACCTGGCCGGGGTAGATCTTGTCCGGATGGCTGAGCATCGGCTTGTTGGCCTCGAAGATCTTCATGTAGGCGTTGGCATTGCCGTa                     <  2:3353181/150‑1 (MQ=255)
                                                                                                                               aCGAAAACCCCGCCACGCGGGC‑GGGGTTGGCGAAACGCCGGGATCACTCGGGAATGCGCAGGACCTGGCCGGGGTAGATCTTGTCCGGATGGCTGAGCATCGGCTTGTTGGCCTCGAAGATCTTCATGTAGGCGTTGGCATTGCCGTAtt                   >  2:5482341/1‑150 (MQ=255)
                                                                                                                               aCGAAAACCCCGCCACGCGGGC‑GGGGTTGGCGAAACGCCGGGATCACTCGGGAATGCGCAGGACCTGGCCGGGGTAGATCTTGTCCGGATGGCTGAGCATCGGCTTGTTGGCCTCGAAGATCTTCATGTAGGCGTTGGCATTGCCGTAtt                   >  1:7014552/1‑150 (MQ=255)
                                                                                                                                cGAAAACCCCGCCACGCGGGC‑GGGGTTGGCGAAACGCCGGGATCACTCGGGAATGCGCAGGACCTGGCCGGGGTAGATCTTGTCCGGATGGCTGAGCATCGGCTTGTTGGCCTCGAAGATCTTCATGTAGGCGTTGGCATTGCCGTATTc                  >  1:2958739/1‑150 (MQ=255)
                                                                                                                                    aaCCCCGCCACGCGGGC‑GGGGTTGGCGAAACGCCGGGATCACTCGGGAATGCGCAGGACCTGGCCGGGGTAGATCTTGTCCGGATGGCTGAGCATCGGCTTGTTGGCCTCGAAGATCTTCATGTAGGCGTTGGCATTGCCGTATTCGGcc              <  1:10483249/150‑1 (MQ=255)
                                                                                                                                        ccGCCTCGCGGGC‑GGGGTTGGCGAAACGCCTGTATCACTCGGGAATGCGCAGGACCTGGCCGGTGTAGATCTTGTCCGGATGGCTGAGCATCGGCTTGTTGGACTCGAAGATCTTCATGTAGGCGTTGGCATTGCCGTATTCGGCCTTgg          <  2:8473670/150‑1 (MQ=255)
                                                                                                                                        ccGCCACGCGGGC‑GGGGTTGGCGAAACGCCGGGATCACTCGGGAATGCGCAGGACCTGGCCGGGGTAGATCTTGTCCGGATGGCTGAGCATCGGCTTGTTGGCCTCGAAGATCGTCATGTAGGCGTTGGCATTGCCGTATTCGGCCTTgg          >  2:6070129/1‑150 (MQ=255)
                                                                                                                                          gCCACGCGGGC‑GGGGTTGGCGAAACGCCGGGATCACTCGGGAATGCGCAGGACCTGGCCGGGGTAGATCTTGTCCGGATGGCTGAGCATCGGCTTGTTGGCCTCGAAGATCTTCATGTAGGCGTTGGCATTGCCGTATTCGGCCTTGGCg        <  1:61753/150‑1 (MQ=255)
                                                                                                                                          gCCACGCGGGC‑GGGGTTGGCGAAACGCCGGGATCACTCGGGAATGCGCAGGACCTGGCCGGGGTAGATCTTGTCCGGATGGCTGAGCATCGGCTTGTTGGCCTCGAAGATCTTCATGTAGGCGTTGGCATTGCCGTATTCGGCCTTGGCg        >  2:10694973/1‑150 (MQ=255)
                                                                                                                                           ccACGCGGGC‑GGGGTTGGCGAAACGCCGGGATCACTCGGGAATGCGCAGGACCTGGCCGGGGTAGATCTTGTCCGGATGGCTGAGCATCGGCTTGTTGGCCTCGAAGATCTTCATGTAGGCGTTGGCATTGCCGTATTCGGCCTTGGCGa       <  2:9836797/150‑1 (MQ=255)
                                                                                                                                           ccACGCGGGC‑GGGGTTGGCGAAACGCCGGGAACACTCGGGACTGCGCAGGACCTGGCCGGGGTAGATCTTGTCCGGATGGCTGAGCATCGGCTTGTTGGCCTCGAAGATCTTCATGTAGGCGTTGGCATTGCCGTATTCGGCCTTGGCGa       <  2:10401009/150‑1 (MQ=255)
                                                                                                                                            cACGCGGGC‑GGGGTTGGCGAAACGCCGGGATCACTCGGGAATGCGCAGGACCTGGCCGGGGTAGATCTTGTCCGGATGGCTGAGCATCGGCTTGTTGGCCTCGAAGATCTTCATGTAGGCGTTGGCATTGCCGTATTCGGCCTTGGCGAt      >  1:8021741/1‑150 (MQ=255)
                                                                                                                                              cgcgGGC‑GGGGTTGGCGAAACGCCGGGATCACTCGGGAATGCGCAGGACCTGGCCGGGGTAGATCTTGTCCGGATGGCTGAGCATCGGCTTGTTGGCCTCGAAGATCTTCATGTAGGCGTTGGCATTGCCGTATTCGGCCTTGGCGATgg    <  2:5998463/150‑1 (MQ=255)
                                                                                                                                               gcgggc‑ggGGTTGGCGAAACGCCGGGATCACTCGGGAATGCGCAGGACCTGGCCGGGGTAGATCTTGTCCGGATGGCTGAGCATCGGCTTGTTGGCCTCGAAGATCTTCATGTAGGCGTTGGCATTGCCGTATTCGGCCTTGGCGATGgc   <  2:4691097/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                 
CCTATGGTGTCGCCCACCTGTTGGCGGTACGCCACCCGGACAGCCGTGGCCAGGTCCGGGACACCGAGGAGTTCGCTGCCCAGGAGGACTACCGGCGGCTGCTCGAAGGACTGCCGTAGCCGCGGAAACGAAAACCCCGCCACGCGGGCGGGGGTTGGCGAAACGCCGGGATCACTCGGGAATGCGCAGGACCTGGCCGGGGTAGATCTTGTCCGGATGGCTGAGCATCGGCTTGTTGGCCTCGAAGATCTTCATGTAGGCGTTGGCATTGCCGTATTCGGCCTTGGCGATGGCG  >  NC_008463/6102356‑6102650

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑