Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 1,441,861 (C)6→4 intergenic (+31/+11) mexK → / ← PA14_RS06750 multidrug efflux RND transporter permease subunit MexK/DUF4197 domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084631,441,8570C.98.9% 1680.7 / 11.8 347intergenic (+27/+16)mexK/PA14_RS06750multidrug efflux RND transporter permease subunit MexK/DUF4197 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base C (1/3);  new base . (140/203);  total (141/206)
Fisher's exact test for biased strand distribution p-value = 6.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01
*NC_0084631,441,8580C.99.1% 1681.5 / 8.6 347intergenic (+28/+15)mexK/PA14_RS06750multidrug efflux RND transporter permease subunit MexK/DUF4197 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base C (0/1);  major base . (140/203);  minor base G (1/2);  total (141/206)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 412 total aligned reads displayed.

TTCGGGCCGATGGCGGTGGCGATCATGGGCGGGCTGATCGTCGCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCGCCCCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCATCTCGAAGAGCCCGTCGAGGGCCTGTTCGGTGACATAGCCCTC  >  NC_008463/1441708‑1442004
                                                                                                                                                     ||                                                                                                                                                  
  cGGGCCGATGGCGGTGGCGATCATGGGCGGGCTGATCGTCGCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCGccc                                                                                                                                                   >  2:1850869/1‑150 (MQ=255)
   gggCCGATGGCGGTGGCGATCATGGGCGGGCTGATCGTCGCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCGcccc                                                                                                                                                  <  2:5484779/150‑1 (MQ=255)
      ccGATGGCGGTGGCGATCATGGGCGGGCTGATCGTCGCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCGccccgtc                                                                                                                                               >  1:27930/1‑147 (MQ=255)
       cGATGGCGGTGGCGATCATGGGCGGGCTGATCGTCGCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCGccccgtcc                                                                                                                                              <  2:7342445/150‑5 (MQ=255)
       cGATGGCGGTGGCGATCATGGGCGGGCTGATCGTCGCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCGccccgtcc                                                                                                                                              <  1:8622772/150‑5 (MQ=255)
       cGATGGCGGTGGCGATCATGGGCGGGCTGATCGTCGCCACCGTGCTCACCATGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCGccccgtcc                                                                                                                                              <  2:8260710/150‑5 (MQ=255)
         aTGGCGGTGGCGATCATGGGCGGGCTGATCGTCGCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGGTCGCGccccgtccgg                                                                                                                                            >  2:7112260/1‑144 (MQ=255)
         aTGGCGGTGGCGATCATGGGCGGGCTGATCGTCGCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCGccccgtccgt                                                                                                                                            <  2:7363633/150‑7 (MQ=255)
          tGGCGGTGGCGATCATGGGCGGGCTGATCGTCGCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCGccccgtccgtc                                                                                                                                           >  1:8040642/1‑143 (MQ=255)
          tGGCGGTGGCGATCATGGGCGGGCTGATCGTCGCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCGccccgtccggc                                                                                                                                           >  2:979406/1‑143 (MQ=255)
           ggCGGTGGCGATCATGGGCGGGCTGATCGTCGCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCg                                                                                                                                        >  1:7351369/1‑150 (MQ=255)
            gCGGTGGCGATCATGGGCGGGCTGATCGTCGCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGa                                                                                                                                       >  1:9352798/1‑150 (MQ=255)
              ggTGGCGATCATGGGCGGGCTGATCGTCGCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACt                                                                                                                                     >  1:1559817/1‑150 (MQ=255)
                   cGATCATGGGCGGGCTGATCGTCGCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCt                                                                                                                                <  2:1513130/150‑1 (MQ=255)
                     aTCATGGGCGGGCTGATCGTCGCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTgg                                                                                                                              >  1:6762882/1‑150 (MQ=255)
                     aTCATGGGCGGGCTGATCGTCGCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTgg                                                                                                                              >  2:7769301/1‑150 (MQ=255)
                      tCATGGGCGGGCTGATCGTCGCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGc                                                                                                                             <  1:1306268/150‑1 (MQ=255)
                      tCATGGGCGGGCTGATCGTCGCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGc                                                                                                                             <  2:10323507/150‑1 (MQ=255)
                          gggcgggcTGATCGTCGCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCcgcg                                                                                                                         >  2:10981563/1‑150 (MQ=255)
                           ggcgggcTGATCGTCGCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCgcgc                                                                                                                        <  2:10579184/150‑1 (MQ=255)
                           ggcgggcTGATCGTAGCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCGTGGTTCCGTGTGAAGAAGGACGAGGCGAGGGACTGACGGGAGACGGTGAAGGGGCGATCGCG‑‑CCCCGTCCGTCGAGGCACCTGGCCgcgc                                                                                                                        >  2:1981438/1‑150 (MQ=255)
                            gcgggcTGATCGTCGCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGcc                                                                                                                       >  1:3713196/1‑150 (MQ=255)
                                   gATCGTCGCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTTTGCCGCGTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACGCACCTGGCCGCGCCGAAGAcc                                                                                                                <  1:5588434/150‑1 (MQ=255)
                                    aTCGTCGCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACct                                                                                                               <  1:10040838/150‑1 (MQ=255)
                                       gtcgCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACcttct                                                                                                            <  1:3179473/150‑1 (MQ=255)
                                        tcgCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACcttctt                                                                                                           >  2:3876118/1‑150 (MQ=255)
                                         cgCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTg                                                                                                          <  1:4428915/150‑1 (MQ=255)
                                         cgCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTg                                                                                                          <  2:8013837/150‑1 (MQ=255)
                                           ccACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGc                                                                                                        >  1:10230003/1‑150 (MQ=255)
                                           ccACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTACCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGc                                                                                                        <  1:5739343/150‑1 (MQ=255)
                                            cACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGcc                                                                                                       >  1:121229/1‑150 (MQ=255)
                                            cACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGcc                                                                                                       >  2:2971877/1‑150 (MQ=255)
                                             aCCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTGGGCCa                                                                                                      >  1:6659350/1‑150 (MQ=255)
                                              ccGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAg                                                                                                     <  1:10424397/150‑1 (MQ=255)
                                                 tGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGcc                                                                                                  <  1:2408924/150‑1 (MQ=255)
                                                   cTCACCATGCTGTTCCTGCCTGCGCTCTATGCCGACTGGTTCCGAGTGAAGAAGTACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCACGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTACTTGGCCAGGCCgg                                                                                                >  2:6424605/1‑150 (MQ=255)
                                                    tCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGTAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGt                                                                                               <  2:7286840/150‑1 (MQ=255)
                                                      aCCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTgg                                                                                             <  1:362024/150‑1 (MQ=255)
                                                       cccTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTggc                                                                                            <  1:3509125/150‑1 (MQ=255)
                                                          tgctgTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTggcggc                                                                                         <  2:254825/150‑1 (MQ=255)
                                                           gctgTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGgcc                                                                                        >  1:4605412/1‑150 (MQ=255)
                                                           gctgTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGgcc                                                                                        <  2:4452202/150‑1 (MQ=255)
                                                           gctgTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCGTCTTGGCCAGGCCGGTGGCGgcc                                                                                        >  2:5382203/1‑150 (MQ=255)
                                                             tgTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGgccgc                                                                                      >  1:11034598/1‑150 (MQ=255)
                                                                   tGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCgg                                                                                >  1:7456275/1‑150 (MQ=255)
                                                                   tGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCgg                                                                                <  1:7940867/150‑1 (MQ=255)
                                                                   tGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCgg                                                                                <  1:7172330/150‑1 (MQ=255)
                                                                     ccGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGt                                                                              >  1:2334186/1‑150 (MQ=255)
                                                                        gcgcTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGACGGGGACGGGGCGATCCCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCt                                                                           <  2:8633192/150‑1 (MQ=255)
                                                                        gcgcTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCt                                                                           >  2:5813459/1‑150 (MQ=255)
                                                                         cgcTCTATGCCGCCTGGTCCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACGCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTg                                                                          <  2:6243304/150‑1 (MQ=255)
                                                                          gcTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTgg                                                                         >  2:5099593/1‑150 (MQ=255)
                                                                           ctctATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGc                                                                        <  1:8900802/150‑1 (MQ=255)
                                                                             ctATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCgg                                                                      >  1:1950456/1‑150 (MQ=255)
                                                                                  ccgccTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGct                                                                 <  2:9275805/150‑1 (MQ=255)
                                                                                    gccTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGctct                                                               >  2:2104720/1‑150 (MQ=255)
                                                                                      cTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCtcttc                                                             >  1:4293627/1‑150 (MQ=255)
                                                                                      cTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCtcttc                                                             >  1:9809915/1‑150 (MQ=255)
                                                                                        ggTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTtctc                                                           >  1:4522213/1‑150 (MQ=255)
                                                                                             cGTGTGCAGACGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCt                                                      <  2:3370786/150‑1 (MQ=255)
                                                                                             cGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCt                                                      >  1:9035472/1‑150 (MQ=255)
                                                                                              gtgtGAAGAAGGACGAGGCGCGGGCCTGACGGGAGATGGGGACGGGGCGATCGCG‑‑CCCCGTCCGGCGACTCACCTGGCCGCGCCGAAGTCCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGGTCTGGCGGAGGCTCTTCTCCTGCtt                                                     >  1:6592063/1‑150 (MQ=255)
                                                                                              gtgtGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCtt                                                     <  1:6128707/150‑1 (MQ=255)
                                                                                              gtgtGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCtt                                                     >  2:3537712/1‑150 (MQ=255)
                                                                                               tgtgAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTg                                                    >  2:8510699/1‑150 (MQ=255)
                                                                                                gtgAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTgg                                                   <  1:590315/150‑1 (MQ=255)
                                                                                                 tgAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGc                                                  >  2:163880/1‑150 (MQ=255)
                                                                                                  gaagaaGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCg                                                 <  1:7018750/150‑1 (MQ=255)
                                                                                                    agaagTACGTGGGGCGGGCCTGACGGGAGTTGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCAGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGat                                               <  1:10780575/150‑1 (MQ=255)
                                                                                                    agaagGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGat                                               >  1:9975740/1‑150 (MQ=255)
                                                                                                    agaagGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGat                                               >  2:5434195/1‑150 (MQ=255)
                                                                                                        ggACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGA‑‑GCGCGCCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGTTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGatcatc                                           <  2:6536372/150‑1 (MQ=255)
                                                                                                          aCGAGGCGCGGGCCGGACGGGAGAAGGGGACGGGGCGA‑‑GCGCGCCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCAtctc                                         <  2:6743649/150‑1 (MQ=255)
                                                                                                             aGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCATCTCGaa                                      >  2:4602183/1‑150 (MQ=255)
                                                                                                               gcgcGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCATCTCGAaga                                    <  2:4255532/150‑1 (MQ=255)
                                                                                                                    ggCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCATCTCGAAGAGCCCg                               >  2:7692960/1‑150 (MQ=255)
                                                                                                                    ggCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCATCTCGAAGAGCCCg                               >  2:7480588/1‑150 (MQ=255)
                                                                                                                    ggCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCATCTCGAAGAGCCCg                               <  1:6265462/150‑1 (MQ=255)
                                                                                                                     gCCTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCATCTCGAAGAGCCCGt                              <  1:7377514/150‑1 (MQ=255)
                                                                                                                      ccTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCATCTCGAAGAGCCCGTc                             <  2:6932377/150‑1 (MQ=255)
                                                                                                                       cTGACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCATCTCGAAGAGCCCGTCg                            >  1:7739961/1‑150 (MQ=255)
                                                                                                                         gACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCATCTCGAAGAGCCCGTCGAg                          <  1:8261763/150‑1 (MQ=255)
                                                                                                                         gACGGGAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCGTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCATCTCGAAGAGCCCGTCGAg                          >  2:5997594/1‑150 (MQ=255)
                                                                                                                            gggAGAAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCATCTCGAAGAGCCCGTCGAGGGc                       >  1:10967465/1‑150 (MQ=255)
                                                                                                                               agaAGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCATCTCGAAGAGCCCGTCGAGGGCCTg                    <  1:7803713/150‑1 (MQ=255)
                                                                                                                                 aaGGGGACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGACGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCATCTCGAAGAGCCCGTCGAGGGCCTGtt                  <  2:8817850/150‑1 (MQ=255)
                                                                                                                                  aGGGGACGGGGCGATCGCG‑‑CCCCGACCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCATCTCGAAGAGCCCGTCGAGGGCCTGTTc                 <  2:10833765/150‑1 (MQ=255)
                                                                                                                                   ggggACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGCTCAGCTCGAAGAGCCCGTCGAGGGCCTGTTCg                >  2:2350646/1‑150 (MQ=255)
                                                                                                                                   ggggACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCATCTCGAAGAGCCCGTCGAGGGCCTGTTCg                <  2:3116811/150‑1 (MQ=255)
                                                                                                                                   ggggACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCATCTCGAAGAGCCCGTCGAGGGCCTGTTCg                <  1:1878645/150‑1 (MQ=255)
                                                                                                                                      gACGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCATCTCGAAGAGCCCGTCGAGGGCCTGTTCGGTg             <  1:8515184/150‑1 (MQ=255)
                                                                                                                                        cGGGGCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCATCTCGAAGAGCCCGTCGAGGGCCTGTTCGGTGAc           >  2:9493697/1‑150 (MQ=255)
                                                                                                                                         ggggCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCATCTCGAAGAGCCCGTCGAGGGCCTGTTCGGTGACa          >  2:2177161/1‑150 (MQ=255)
                                                                                                                                         ggggCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCATCTCGAAGAGCCCGTCGAGGGCCTGTTCGGTGACa          >  1:6074382/1‑150 (MQ=255)
                                                                                                                                           ggCGATCGCG‑‑CCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCATCTCGAAGAGCCCGTCGAGGGCCTGTTCGGTGACATa        >  1:3005757/1‑150 (MQ=255)
                                                                                                                                                gatcgcgCCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCATCTCGAAGAGCCCGTCGAGGGCCTGTTCGGTGACATAGcc     <  1:5078581/147‑1 (MQ=255)
                                                                                                                                                  tcgcgCCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCATCTCGAAGAGCCCGTCGAGGGCCTGTTCGGTGACATAGCCCt   >  1:9080660/2‑150 (MQ=255)
                                                                                                                                                  tcgcgCCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCATCTCGAAGAGCCCGTCGAGGGCCTGTTCGGTGACATAGCCCt   >  1:7237933/2‑150 (MQ=255)
                                                                                                                                                   cgcgCCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCATCTCGAAGAGCCCGTCGAGGGCCTGTTCGGTGACATAGCCCTc  <  1:10537921/150‑1 (MQ=255)
                                                                                                                                                   cgcgCCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCATCTCGAAGAGCCCGTCGAGGGCCTGTTCGGTGACATAGCCCTc  >  1:9407145/1‑150 (MQ=255)
                                                                                                                                                     ||                                                                                                                                                  
TTCGGGCCGATGGCGGTGGCGATCATGGGCGGGCTGATCGTCGCCACCGTGCTCACCCTGCTGTTCCTGCCGGCGCTCTATGCCGCCTGGTTCCGTGTGAAGAAGGACGAGGCGCGGGCCTGACGGGAGAAGGGGACGGGGCGATCGCGCCCCCCGTCCGTCGACTCACCTGGCCGCGCCGAAGACCTTCTTGGCCAGGCCGGTGGCGGCCGCCGCCGGGTTCTGGCGGATGCTCTTCTCCTGCTTGGCGATCATCTCGAAGAGCCCGTCGAGGGCCTGTTCGGTGACATAGCCCTC  >  NC_008463/1441708‑1442004

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑