BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_008463	3,670,543	+A	coding (543/1020 nt)	PA14_RS16675 →	ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_008463	3,670,542	1	.	A	98.7%	1520.0 / 8.4	373	coding (542/1020 nt)	PA14_RS16675	ABC transporter permease
Reads supporting (aligned to +/- strand): ref base . (5/0); new base A (179/189); total (184/189)
Fisher's exact test for biased strand distribution p-value = 2.84e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 403 total aligned reads displayed.

ACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCGTTTTCCTGG‑AGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGCCGAGTTCCTCCGCGG  >  NC_008463/3670392‑3670686

aCGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTgg                                                                                                                                                   >  1:4514850/1‑150 (MQ=255)

aCGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTgg                                                                                                                                                   >  1:1420041/1‑150 (MQ=255)

 cGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGG‑a                                                                                                                                                 >  1:5428938/1‑150 (MQ=255)

 cGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGG‑a                                                                                                                                                 <  2:3515791/150‑1 (MQ=255)

 cGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGG‑a                                                                                                                                                 <  1:7298377/150‑1 (MQ=255)

  gTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGG‑aa                                                                                                                                                <  1:4829263/150‑2 (MQ=255)

   tGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGG‑aag                                                                                                                                               >  2:2530784/1‑148 (MQ=255)

    gCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGG‑aagt                                                                                                                                              >  2:10622986/1‑147 (MQ=255)

      tCGCCCGGGTGAGTTACGGCTTCCGCGTGTCGATCCTCTGCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGG‑aagtct                                                                                                                                            <  2:10791002/150‑6 (MQ=255)

         cccGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGt                                                                                                                                          <  1:8218110/150‑1 (MQ=255)

          ccGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTc                                                                                                                                         >  1:57564/1‑150 (MQ=255)

            gggTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCgg                                                                                                                                       <  2:8567759/150‑1 (MQ=255)

            gggTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCgg                                                                                                                                       >  2:5571608/1‑150 (MQ=255)

                gATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCt                                                                                                                                   >  2:2030974/1‑150 (MQ=255)

                gATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGACAGCG‑TTTCCTGGAAGTCTGGTCGGGGCt                                                                                                                                   >  1:6727956/1‑150 (MQ=255)

                gATTTACGGCTTCCGCGTGTCGATCCTCGTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCt                                                                                                                                   <  2:6217024/150‑1 (MQ=255)

                  tttACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGc                                                                                                                                 <  1:236910/150‑1 (MQ=255)

                  tttACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGc                                                                                                                                 <  2:6819788/150‑1 (MQ=255)

                   ttACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGcc                                                                                                                                <  2:8535618/150‑1 (MQ=255)

                    tACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCg                                                                                                                               >  2:8697117/1‑150 (MQ=255)

                    tACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCg                                                                                                                               >  1:10392611/1‑150 (MQ=255)

                     aCGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCgg                                                                                                                              >  1:181090/1‑150 (MQ=255)

                       ggCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTg                                                                                                                            >  1:4341993/1‑150 (MQ=255)

                        gCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGc                                                                                                                           <  1:8264486/150‑1 (MQ=255)

                          ttCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGctc                                                                                                                         <  1:9806737/150‑1 (MQ=255)

                          ttCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGctc                                                                                                                         >  2:2359929/1‑150 (MQ=255)

                                 tgtCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACctgc                                                                                                                  >  2:155607/1‑150 (MQ=255)

                                 tgtCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACctgc                                                                                                                  >  1:2067626/1‑150 (MQ=255)

                                     gATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGat                                                                                                              <  2:6002456/150‑1 (MQ=255)

                                      aTCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGatc                                                                                                             >  2:5320698/1‑150 (MQ=255)

                                       tCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGatca                                                                                                            >  1:7087380/1‑150 (MQ=255)

                                         ctctTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCGTTTCCGGGA‑AGTCTGGTCGGGGCTGCCGGTGCTCTCCCTGCTGatcatc                                                                                                          >  2:8417577/1‑150 (MQ=255)

                                             tCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTgg                                                                                                      >  2:4014338/1‑150 (MQ=255)

                                             tCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTgg                                                                                                      >  1:2935007/1‑150 (MQ=255)

                                                    gaccctgaccGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCtt                                                                                               >  1:9382408/1‑150 (MQ=255)

                                                    gaccctgaccGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCtt                                                                                               >  1:10004985/1‑150 (MQ=255)

                                                    gaccctgaccGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCtt                                                                                               <  1:6598240/150‑1 (MQ=255)

                                                     accctgaccGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTCCGGCGGCTGGGTCGACCTGCTCGGCCCGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTc                                                                                              <  2:9772198/150‑1 (MQ=255)

                                                         tgaccGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTcc                                                                                          <  2:4362995/150‑1 (MQ=255)

                                                           accGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCat                                                                                        <  2:10981639/150‑2 (MQ=255)

                                                            ccGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGc                                                                                       >  2:2427175/1‑150 (MQ=255)

                                                            ccGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGc                                                                                       <  1:893956/150‑1 (MQ=255)

                                                            ccGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACGACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGc                                                                                       <  2:4871279/150‑1 (MQ=255)

                                                              gTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCg                                                                                     >  2:3102068/1‑150 (MQ=255)

                                                               tGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGa                                                                                    >  1:74747/1‑150 (MQ=255)

                                                               tGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGa                                                                                    >  2:505432/1‑150 (MQ=255)

                                                               tGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGa                                                                                    >  1:3138377/1‑150 (MQ=255)

                                                                gCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGaa                                                                                   >  2:1429219/1‑150 (MQ=255)

                                                                   cAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACtt                                                                                <  1:8409345/150‑1 (MQ=255)

                                                                    aGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTc                                                                               <  2:1017843/150‑1 (MQ=255)

                                                                     gTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCt                                                                              >  2:7367618/1‑150 (MQ=255)

                                                                     gTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCt                                                                              >  2:183231/1‑150 (MQ=255)

                                                                      ttCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCtg                                                                             <  2:8204493/150‑1 (MQ=255)

                                                                        ctctGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCtggt                                                                           <  2:9041482/150‑1 (MQ=255)

                                                                           tGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGgc                                                                        >  1:7619167/1‑150 (MQ=255)

                                                                             aTCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGgctg                                                                      >  2:5137376/1‑150 (MQ=255)

                                                                               cgtcGTCCCCGTCGGCGCCCAGCAAGGCTACGACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑ATTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGgctgct                                                                    <  2:7347725/147‑1 (MQ=255)

                                                                                 gCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTgg                                                                  >  1:7956047/1‑150 (MQ=255)

                                                                                    tCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGca                                                               >  2:9383536/1‑150 (MQ=255)

                                                                                      aCCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGcatc                                                             >  1:9491727/1‑150 (MQ=255)

                                                                                      aCCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGcatc                                                             >  1:4053948/1‑150 (MQ=255)

                                                                                      aCCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGcatc                                                             >  1:357216/1‑150 (MQ=255)

                                                                                      aCCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGcatc                                                             >  1:1175458/1‑150 (MQ=255)

                                                                                        cGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGcatcat                                                           >  1:10725808/1‑150 (MQ=255)

                                                                                        cGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGcatcat                                                           >  2:3364378/1‑150 (MQ=255)

                                                                                         gTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCAtg                                                          <  2:2216850/150‑1 (MQ=255)

                                                                                           cGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCAtgct                                                        <  2:3861607/150‑1 (MQ=255)

                                                                                             gcgcCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCAtgctgc                                                      <  2:5391168/150‑1 (MQ=255)

                                                                                             gcgcCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCAtgctgc                                                      >  1:3655110/1‑150 (MQ=255)

                                                                                             gcgcCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCAtgctgc                                                      <  2:2763821/150‑1 (MQ=255)

                                                                                              cgcCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATgctgct                                                     <  2:9519667/150‑1 (MQ=255)

                                                                                                cccTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGt                                                   <  1:2025716/150‑1 (MQ=255)

                                                                                                    gCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTc                                               <  1:8592500/150‑1 (MQ=255)

                                                                                                        ggCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTgg                                           <  1:377544/150‑1 (MQ=255)

                                                                                                          ctacgaCGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGAt                                         <  2:118652/150‑1 (MQ=255)

                                                                                                           tactacGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATg                                        <  2:638783/150‑1 (MQ=255)

                                                                                                              tacGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGc                                     <  1:4968015/150‑1 (MQ=255)

                                                                                                                cggcggCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCt                                   <  2:10045417/150‑1 (MQ=255)

                                                                                                                    ggcTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCtggtg                               >  2:4583804/1‑150 (MQ=255)

                                                                                                                    ggcTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCtggtg                               >  1:7789470/1‑150 (MQ=255)

                                                                                                                      cTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGa                             <  1:10157431/150‑1 (MQ=255)

                                                                                                                       tGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGAc                            <  1:284920/150‑1 (MQ=255)

                                                                                                                       tGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGAc                            >  1:8079394/1‑150 (MQ=255)

                                                                                                                           tCGACCTGCTCGGCCAGCG‑GTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTgg                        <  2:758506/150‑1 (MQ=255)

                                                                                                                            cGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGt                       >  1:9250455/1‑150 (MQ=255)

                                                                                                                            cGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGt                       >  2:28175/1‑150 (MQ=255)

                                                                                                                            cGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGt                       <  1:1854898/150‑1 (MQ=255)

                                                                                                                            cGACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGt                       >  2:29419/1‑150 (MQ=255)

                                                                                                                             gACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTc                      <  1:6011271/150‑1 (MQ=255)

                                                                                                                             gACCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTc                      <  2:7687157/150‑1 (MQ=255)

                                                                                                                              aCCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTcc                     >  1:344923/1‑150 (MQ=255)

                                                                                                                              aCCTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTcc                     >  2:7011919/1‑150 (MQ=255)

                                                                                                                               ccTGCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCcg                    <  1:2789083/150‑1 (MQ=255)

                                                                                                                                  gCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCgcgc                 <  1:567595/150‑1 (MQ=255)

                                                                                                                                  gCTCGGCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCgcgc                 <  1:1067457/150‑1 (MQ=255)

                                                                                                                                      ggCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGCCGAg             <  1:8744859/150‑1 (MQ=255)

                                                                                                                                      ggCCAGCG‑TTTCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGCCGAg             >  2:5799088/1‑150 (MQ=255)

                                                                                                                                             cgtttCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGCCGAGTtcctc       <  2:1343499/148‑1 (MQ=255)

                                                                                                                                             cgtttCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGCCGAGTtcctc       <  2:7908199/148‑1 (MQ=255)

                                                                                                                                               tttCCTGGAAGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGCCGAGTTCCTCcg     >  1:6298828/1‑150 (MQ=255)

ACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCGTTTTCCTGG‑AGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGCCGAGTTCCTCCGCGG  >  NC_008463/3670392‑3670686

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`