BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_008463	559,051	+T	intergenic (+619/+70)	PA14_RS02545 → / ← rarD	glycine cleavage system protein R/EamA family transporter RarD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_008463	559,050	1	.	T	97.7%	1428.1 / 19.5	353	intergenic (+618/+71)	PA14_RS02545/rarD	glycine cleavage system protein R/EamA family transporter RarD
Reads supporting (aligned to +/- strand): ref base . (7/1); new base T (183/160); total (191/162)
Fisher's exact test for biased strand distribution p-value = 7.46e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 380 total aligned reads displayed.

CAGCGGTGTGCTCAAAGACTGATCAGGCGCTTTGCAGGGCTTCTTCCGACGAGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑A‑T‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTGGGGAAGGCGGCGCAGGCTGCGCCAGGCGTCGAAGCTGTAGACGGCCAGGGCGAGCCAGATGA  >  NC_008463/558901‑559194

cAGCGGTGTGCTCAAAGACTGATCAGGCGCTTTGCAGGGCTTCTTCCGACGAGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAAGCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑a                                                                                                                                                    >  2:7644241/1‑150 (MQ=255)

cAGCGGTGTGCTCAAAGACTGATCAGGCGCTTTGCAGGGCTTCTTCCGACGAGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑a                                                                                                                                                    >  1:7549493/1‑150 (MQ=255)

cAGCGGTGTGCTCAAAGACTGATCAGGCGCTTTGCAGGGCTTCTTCCGACGAGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑a                                                                                                                                                    >  1:1932473/1‑150 (MQ=255)

 aGCGGTGTGCTCAAAGACTGATCAGGCGCTTTGCAGGGCTTCTTCCGACGAGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑A‑t                                                                                                                                                  >  2:6214711/1‑150 (MQ=255)

   cGGTGTGCTCAAAGACTGATCAGGCGCTTTGCAGGGCTTCTTCCGACGAGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑A‑ttg                                                                                                                                                >  2:9899092/1‑148 (MQ=255)

   cGGTGTGCTCAAAGACTGATCAGGCGCTTTGCAGGGCTTCTTCCGACGAGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑A‑ttg                                                                                                                                                <  2:6939992/150‑3 (MQ=255)

     gtgtGCTCAAAGACTGATCAGGCGCTTTGCAGGGCTTCTTCCGACGAGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑A‑ttggc                                                                                                                                              >  2:10909818/1‑146 (MQ=255)

        tgCTCAAAGACTGATCAGGCGCTTTGCAGGGCTTCTTCCGACGAGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCggg                                                                                                                                           >  2:6868417/1‑150 (MQ=255)

        tgCTCAAAGACTGATCAGGCGCTTTGCAGGGCTTCTTCCGACGAGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCggg                                                                                                                                           >  2:10433445/1‑150 (MQ=255)

           tCAAAGACTGATCAGGCGCTTTGCAGGGCTTCTTCCGACGAGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGa                                                                                                                                        <  1:10612145/150‑1 (MQ=255)

                 aCTGATCAGGCGCTTTGCAGGGCTTCTTCCGACGAGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATaggaa                                                                                                                                  <  2:4865827/150‑1 (MQ=255)

                  cTGATCAGGCGCTTTGCAGGGCTTCTTCCGACGAGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATaggaag                                                                                                                                 >  2:6085446/1‑150 (MQ=255)

                   tGATCAGGCGCTTTGCAGGGCTTCTTCCGACGAGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATaggaagg                                                                                                                                <  2:5830570/150‑1 (MQ=255)

                   tGATCAGGCGCTTTGCAGGGCTTCTTCCGACGAGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATaggaagg                                                                                                                                <  1:6027474/150‑1 (MQ=255)

                   tGATCAGGCGCTTTGCAGGGCTTCTTCCGACGAGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATaggaagg                                                                                                                                <  1:5330974/150‑1 (MQ=255)

                   tGATCAGGCGCTTTGCAGGGCTTCTTCCGACGAGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATaggaagg                                                                                                                                <  2:438101/150‑1 (MQ=255)

                      tCAGGCGCTTTGCAGGGCTTCTTCCGACGAGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGt                                                                                                                             <  1:3276837/150‑1 (MQ=255)

                      tCAGGCGCTTTGCAGGGCTTCTTCCGACGAGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGt                                                                                                                             >  1:2521293/1‑150 (MQ=255)

                       cAGGCGCTTTGCAGGGCTTCTTCCGACGAGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTg                                                                                                                            <  1:9161509/150‑1 (MQ=255)

                        aGGCGCTTTGCAGGGCTTCTTCCGACGAGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGTCG‑T‑GGCGGGGGATAGGAAGGAGTGc                                                                                                                           >  2:7995472/1‑150 (MQ=255)

                        aGGCGCTTTGCAGGGCTTCTTCCGACGAGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGc                                                                                                                           <  1:6522338/150‑1 (MQ=255)

                         ggCGCTTTGCAGGGCTTCTTCCGACGAGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCg                                                                                                                          >  1:1240688/1‑150 (MQ=255)

                          gcgcTTTGCAGGGCTTCTTCCGACGAGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGACGGAGTGCgt                                                                                                                         >  1:10032746/1‑150 (MQ=255)

                          gcgcTTTGCAGGGCTTCTTCCGACGAGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCgt                                                                                                                         <  1:1094551/150‑1 (MQ=255)

                             cTTTGCAGGGCTTCTTCCGACGAGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGtt                                                                                                                      <  1:7177010/150‑1 (MQ=255)

                                tGCAGGGCTTCTTCCGACGAGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGttttt                                                                                                                   <  2:9436236/150‑1 (MQ=255)

                                 gCAGGGCTTCTTCCGACGAGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACACAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGtttttt                                                                                                                  >  2:5952820/1‑150 (MQ=255)

                                     ggCTTCTTCCGACGAGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGcc                                                                                                              >  2:192158/1‑150 (MQ=255)

                                     ggCTTCTTCCGACGAGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGcc                                                                                                              <  2:3929427/150‑1 (MQ=255)

                                       cttcttCCGACGAGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGCTCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAg                                                                                                            <  1:10241732/150‑1 (MQ=255)

                                       cttcttCCGACGAGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAg                                                                                                            >  2:2331990/1‑150 (MQ=255)

                                       cttcttCCGACGAGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAg                                                                                                            <  2:3011207/150‑1 (MQ=255)

                                         tcttcCGACGAGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGc                                                                                                          <  2:5282056/150‑1 (MQ=255)

                                             ccGACGAGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGc                                                                                                      >  1:4987419/1‑150 (MQ=255)

                                             ccGACGAGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGc                                                                                                      <  2:6715928/150‑1 (MQ=255)

                                             ccGACGAGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGc                                                                                                      <  2:6431205/150‑1 (MQ=255)

                                              cgacgaGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCg                                                                                                     <  1:9600134/150‑1 (MQ=255)

                                                acgaGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGa                                                                                                   >  1:4826249/1‑150 (MQ=255)

                                                acgaGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGa                                                                                                   <  2:1286260/150‑1 (MQ=255)

                                                acgaGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑A‑TGGGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGa                                                                                                   >  2:10075439/1‑150 (MQ=255)

                                                 cgaGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAg                                                                                                  <  2:3795654/150‑1 (MQ=255)

                                                 cgaGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAg                                                                                                  >  1:10799506/1‑150 (MQ=255)

                                                  gaGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGt                                                                                                 >  1:5162747/1‑150 (MQ=255)

                                                   aGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGtt                                                                                                <  2:1707102/150‑1 (MQ=255)

                                                     ttATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTcc                                                                                              >  2:6572105/1‑150 (MQ=255)

                                                       aTCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGc                                                                                            <  1:3585985/150‑1 (MQ=255)

                                                         ccACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAACGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGTATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGGTCCGCaa                                                                                          <  2:2616487/150‑1 (MQ=255)

                                                         ccACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCaa                                                                                          >  1:444715/1‑150 (MQ=255)

                                                              cgcCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTg                                                                                     >  1:6923369/1‑150 (MQ=255)

                                                              cgcCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTg                                                                                     >  1:2769636/1‑150 (MQ=255)

                                                                      gtgaggatAAGGCATGTCTATTCACAAAGTACGTGCATGAACCAGTGGAAAAGCCTCGGGTGTAACCCTGAGCGACAGA‑C‑TAGGCTGGGGATAGGAAGGACTGCGTGAATAAACGCAATGCATGAGTAGAACCTCAAGCCTGGGGaacac                                                                             >  2:3080950/3‑146 (MQ=255)

                                                                      gggAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGAGAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAAc                                                                             >  1:4627519/1‑150 (MQ=255)

                                                                             gaAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGcc                                                                      >  2:7365184/1‑150 (MQ=255)

                                                                             gaAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGcc                                                                      >  2:10970404/1‑150 (MQ=255)

                                                                             gaAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGcc                                                                      <  1:3800682/150‑1 (MQ=255)

                                                                             gaAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGcc                                                                      >  1:7868835/1‑150 (MQ=255)

                                                                               aGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCg                                                                    >  2:4956206/1‑150 (MQ=255)

                                                                               aGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCg                                                                    >  2:4061737/1‑150 (MQ=255)

                                                                               aGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCg                                                                    <  2:1586810/150‑1 (MQ=255)

                                                                               aGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCg                                                                    >  1:6776553/1‑150 (MQ=255)

                                                                                ggCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCgg                                                                   >  1:732305/1‑150 (MQ=255)

                                                                                 gCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGc                                                                  <  2:10477093/150‑1 (MQ=255)

                                                                                  cAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCt                                                                 <  1:5885898/150‑1 (MQ=255)

                                                                                     gTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTggg                                                              <  1:1521515/150‑1 (MQ=255)

                                                                                       cTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTGGGGa                                                            >  1:7739613/1‑150 (MQ=255)

                                                                                           gCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTGGGGAAggc                                                        >  1:6190726/1‑150 (MQ=255)

                                                                                             acaAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTGGGGAAggcgg                                                      <  1:478078/150‑1 (MQ=255)

                                                                                             acaAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTGGGGAAggcgg                                                      <  1:10186751/150‑1 (MQ=255)

                                                                                                aaGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTGGGGAAGGCGgcgc                                                   >  2:1448732/1‑150 (MQ=255)

                                                                                                aaGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTGGGGAAGGCGgcgc                                                   >  2:2412818/1‑150 (MQ=255)

                                                                                                aaGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTGGGGAAGGCGgcgc                                                   >  1:5713623/1‑150 (MQ=255)

                                                                                                 cgTACGTGCAAGAACCTGTGGAAAAGCAGGGGGAGGATCGCTGAGAGTCAGT‑ATT‑GGCGGGGCCGAGGAAGGAGTGCGAGTTTTTTAGCCACTCTTGCGGAGTACCGCAAGCCGGGGGATAACGTCAGCCCGGCTGGGGAAGGCGGCGCa                                                  >  2:4700975/2‑150 (MQ=255)

                                                                                                    aCGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGG‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCATGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTGGGGAAGGCGGCGCAGGc                                               >  2:5555825/1‑150 (MQ=255)

                                                                                                         cATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTGGGGAAGGCGGCGCAGGCTgcgc                                          >  1:2961956/1‑150 (MQ=255)

                                                                                                           tGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTGGGGAAGGCGGCGCAGGCTGCGCCa                                        <  1:8871034/150‑1 (MQ=255)

                                                                                                           tGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTGGGGAAGGCGGCGCAGGCTGCGCCa                                        <  1:2187025/150‑1 (MQ=255)

                                                                                                           tGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTGGGGAAGGCGGCGCAGGCTGCGCCa                                        >  1:4135732/1‑150 (MQ=255)

                                                                                                           tGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTGGGGAAGGCGGCGCAGGCTGCGCCa                                        <  1:8644748/150‑1 (MQ=255)

                                                                                                            gAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTGGGGAAGGCGGCGCAGGCTGCGCCAg                                       >  1:9003014/1‑150 (MQ=255)

                                                                                                            gAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTGTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTGGGGAAGGCGGCGCAGGCTGCGCCAg                                       <  2:223082/150‑1 (MQ=255)

                                                                                                             aaCCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATATCGTCAGCCCGGCTGGGGAAGGCGGCGCAGGCTGCGCCAgg                                      >  2:8597824/1‑150 (MQ=255)

                                                                                                             aaCCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTGGGGAAGGCGGCGCAGGCTGCGCCAgg                                      <  1:6048733/150‑1 (MQ=255)

                                                                                                                    ggAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTGGGGAAGGCGGCGCAGGCTGCGCCAGGCGTCGaa                               >  2:2801249/1‑150 (MQ=255)

                                                                                                                      aaaaGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTGGGGAAGGCGGCGCAGGCTGCGCCAGGCGTCGAAGc                             >  2:9816007/1‑150 (MQ=255)

                                                                                                                      aaaaGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTGGGGAAGGCGGCGCAGGCTGCGCCAGGCGTCGAAGc                             <  1:8518401/150‑1 (MQ=255)

                                                                                                                        aaGCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTGGGGAAGGCGGCGCAGGCTGCGCCAGGCGTCGAAGCTg                           <  2:2149156/150‑1 (MQ=255)

                                                                                                                        aaGCCTGGGGTGGATCGCTGAGCGTCAGG‑ATT‑GGCGGGGGATAGGAAGGAGGGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCGGGGGAAGGCGGCGCAGGCTGCGCCAGGCGTCGAAGCTg                           >  2:3647709/1‑150 (MQ=255)

                                                                                                                          gCCTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTGGGGAAGGCGGCGCAGGCTGCGCCAGGCGTCGAAGCTGTa                         <  2:9277851/150‑1 (MQ=255)

                                                                                                                            cTGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTGGGGAAGGCGGCGCAGGCTGCGCCAGGCGTCGAAGCTGTAGa                       <  1:5634471/150‑1 (MQ=255)

                                                                                                                             tGGGGTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTGGGGAAGGCGGCGCAGGCTGCGCCAGGCGTCGAAGCTGTAGAc                      >  1:5981863/1‑150 (MQ=255)

                                                                                                                             tGGGGTGGATCGCTGAGCGTCAGG‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTGGGGAAGGCGGCGCAGGCTGCGCCAGGCGTCGAAGCTGTAGAc                      >  2:3354076/1‑150 (MQ=255)

                                                                                                                               gggTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTGGGGAAGGCGGCGCAGGCTGCGCCAGGCGTCGAAGCTGTAGACgg                    <  2:2099887/150‑1 (MQ=255)

                                                                                                                                ggTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTGGGGAAGGCGGCGCAGGCTGCGCCAGGCGTCGAAGCTGTAGACGGc                   >  1:9452020/1‑150 (MQ=255)

                                                                                                                                 gTGGATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTGGGGAAGGCGGCGCAGGCTGCGCCAGGCGTCGAAGCTGTAGACGGcc                  <  2:1756557/150‑1 (MQ=255)

                                                                                                                                   ggATCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTGGGGAAGGCGGCGCAGGCTGCGCCAGGCGTCGAAGCTGTAGACGGCCAg                >  2:5400743/1‑150 (MQ=255)

                                                                                                                                      tCGCTGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTGGGGAAGGCGGCGCAGGCTGCGCCAGGCGTCGAAGCTGTAGACGGCCAGGGc             <  2:8803401/150‑1 (MQ=255)

                                                                                                                                          tGAGCGTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTGGGGAAGGCGGCGCAGGCTGCGCCAGGCGTCGAAGCTGTAGACGGCCAGGGCGAGc         >  2:9129047/1‑150 (MQ=255)

                                                                                                                                               gTCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTGGGGAAGGCGGCGCAGGCTGCGCCAGGCGTCGAAGCTGTAGACGGCCAGGGCGAGCCAGAt    <  1:1781912/150‑1 (MQ=255)

                                                                                                                                                tCAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTGGGGAAGGCGGCGCAGGCTGCGCCAGGCGTCGAAGCTGTAGACGGCCAGGGCGAGCCAGATg   >  2:8251769/1‑150 (MQ=255)

                                                                                                                                                 cAGT‑ATT‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTGGGGAAGGAGGCGCAGGCTGCGCCAGGCGTCGAAGCTGTAGACGGCCAGGGCGAGCCAGATGa  >  2:3476643/1‑150 (MQ=255)

CAGCGGTGTGCTCAAAGACTGATCAGGCGCTTTGCAGGGCTTCTTCCGACGAGTTATCCACGCGCCTGCGGGGAGGAGAAGGCAGGTCTATGCACAAAGTACGTGCATGAACCTGTGGAAAAGCCTGGGGTGGATCGCTGAGCGTCAGT‑A‑T‑GGCGGGGGATAGGAAGGAGTGCGTGTTTTTTCGCCAGGCTTGCGGAGTTCCGCAAGCCTGGGGATAACGTCAGCCCGGCTGGGGAAGGCGGCGCAGGCTGCGCCAGGCGTCGAAGCTGTAGACGGCCAGGGCGAGCCAGATGA  >  NC_008463/558901‑559194

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`