Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_008463867,3780AC25.4% 739.5 / 19.3 386L390R (CTG→CGG) PA14_RS04080MFS transporter
Reads supporting (aligned to +/- strand):  ref base A (211/42);  new base C (25/61);  total (237/149)
Fisher's exact test for biased strand distribution p-value = 4.57e-20
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 392 total aligned reads displayed.

CTCTATCCGTCGCACCGGCATCCGTCGACGATCGTCAGGTGCTACCTGGATTTCTGCCAGCAGCACCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGTCTCTCCGCCAAGTAGCGCGCCGAGGCCGATCCCGGCATTGGCGGCGGCGACGTTGCTG  >  NC_008463/867229‑867527
                                                                                                                                                     |                                                                                                                                                     
ctctATCCGTCGCACCGGCATCCGTCGACGATCGTCAGGTGCTACCTGGATTTCTGCCAGCAGCACCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCa                                                                                                                                                       <  1:1671653/150‑1 (MQ=255)
 tctATCCGTCGCACCGGCATCCGTCGACGATCGTCAGGTGCTACCTGGATTTCTGCCAGCAGCACCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAg                                                                                                                                                      >  1:7149355/1‑150 (MQ=255)
    aTCCGTCGCACCGGCATCCGTCGACGATCGTCAGGTGCTACCTGGATTTCTGCCAGCAGCACCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGgcg                                                                                                                                                   >  2:5518256/1‑150 (MQ=255)
         tCGCACCGGCATCCGTCGACGATCGTCAGGTGCTACCTGGATTTCTGCCAGCAGCACCTGGACCTGCTGCACGAGGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTCGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGcga                                                                                                                                              <  1:6268505/150‑1 (MQ=255)
         gcGCACCGGCAGCCGTCGACGATCGTCAGGTGCTACCTGGTTTTCTGCCAGCAGCACCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGcga                                                                                                                                              <  2:6121707/149‑1 (MQ=255)
          cGCACCGGCATCCGTCGACGATCGTCAGGTGCTACCTGGATTTCTGCCAGCAGCACCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGcgac                                                                                                                                             >  2:1590615/1‑150 (MQ=255)
           gCACCGGCATCCGTCGACGATCGTCAGGTGCTACCTGGATTTCTGCCAGCAGCACCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGcgacg                                                                                                                                            >  1:1433552/1‑150 (MQ=255)
            cACCGGCATCCGTCGACGATCGTCAGGTGCTACCTGGATTTCTGCCAGCAGCACCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGcgacga                                                                                                                                           >  1:5407709/1‑150 (MQ=255)
              ccGGCATCCGTCGACGATCGTCAGGTGCTACCTGGATTTCTGCCAGCAGCACCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGacgacg                                                                                                                                         >  1:5567114/1‑150 (MQ=255)
                    tCCGTCGACGATCGTCAGGTGCTACCTGGATTTCTGCCAGCAGCACCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGagc                                                                                                                                   >  2:9789961/1‑150 (MQ=255)
                        tCGACGATCGTCAGGTGCTACCTGGATTTCTGCCAGCAGCACCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGAGCAgcg                                                                                                                               >  1:9036624/1‑150 (MQ=255)
                           acgaTCGTCAGGTGCTACCTGGATTTCTGCCAGCAGCACCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCa                                                                                                                            >  2:6934273/1‑150 (MQ=255)
                           acgaTCGTCAGGTGCTACCTGGATTTCTGCCAGCAGCACCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCa                                                                                                                            >  1:3191278/1‑150 (MQ=255)
                           acgaTCGTCAGGTGCTACCTGGATTTCTGCCAGCAGCACCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCa                                                                                                                            >  2:6261158/1‑150 (MQ=255)
                            cgaTCGTCAGGTGCTACCTGGATTTCTGCCAGCAGCACCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAg                                                                                                                           >  1:3589207/1‑150 (MQ=255)
                              aTCGTCAGGGGCTACCTGTTTTTCGGCCAGCAGCCCGTGCACCGGCGCCACGTTGCCTGTGGGCTGGACGGCCCTGCGCCGGGCGGGGGGTAGGACGCTGGCGTCAGCTTGGTCGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAGAc                                                                                                                         <  2:4260090/150‑1 (MQ=255)
                                tttctggTGCTACCTGTTTTTCTCCCAGCCGCCCTTGGACCTGCTGCACGTGGCCTGTGGGCTGGACGGCCCTGCGCGTGGCGGGGGTTAGGACGCGGGCTTCTGCGTCGTCGCGCCCGGCGCGCGACGACGGGGAGCAGCGCCAGACCg                                                                                                                       <  2:9052583/145‑1 (MQ=255)
                                   cAGGTGCTACCTGGATTTCTGCCAGCAGCACCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCCGACCGAGt                                                                                                                    >  1:4918480/1‑150 (MQ=255)
                                    aGGTGCTACCTGGATTTCTGCCAGCAGCACCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGGGCAGCGCCAGACCGAGTa                                                                                                                   >  1:7495697/1‑150 (MQ=255)
                                    aGGTGCTACCTGGATTTCTGCCAGCAGCACCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCCGACCGAGTa                                                                                                                   >  1:8498681/1‑150 (MQ=255)
                                     ggTGCTACGTGTTTGTCGGCCAGCCGAACGTGGCCCCGCGGCACGAGGCGGGTCGGCGGGCGGGCCCTGCGCGTGGCGGGGGGTAGGCCGCTGGGGTCAGCGTCGGCGCCCCGGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTAc                                                                                                                  <  2:5587707/150‑1 (MQ=255)
                                     ggTGCTACCTGGATTTCTGCCAGCAGCACCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTAc                                                                                                                  >  2:2062624/1‑150 (MQ=255)
                                     ggTGCTACCTGGATTTCTGCCAGCAGCACCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTAc                                                                                                                  >  1:7957221/1‑150 (MQ=255)
                                          tACCTGGATTTCTGCCAGCAGCACCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGa                                                                                                             >  2:3081034/1‑150 (MQ=255)
                                           aCCTGGATTTCTGCCAGCAGCACCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAg                                                                                                            >  1:684425/1‑150 (MQ=255)
                                              tGGTTTTCTGCCCCCAGCACTGGGACGTGCTGCCGGCTGCGTGTCGGGGGGCGGCCCCGGCGCCGGGGGGGGGGTTGGACGGGGGCGTCAGCGTGGTCGCGCCCGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGc                                                                                                         <  2:4905909/150‑1 (MQ=255)
                                              tGGATTTCTGCCAGCAGCACCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAGTCCGAGTACGGCGAGGGc                                                                                                         >  1:10042573/1‑150 (MQ=255)
                                              tGGATTTCTGCCAGCAGCACCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGc                                                                                                         >  2:8207326/1‑150 (MQ=255)
                                                      tGCCAGCAGCACCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGcga                                                                                                 >  1:10762325/1‑150 (MQ=255)
                                                        ccAGCAGCACCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAGTCCGAGTACGGCGAGGGCCGCTGcgacg                                                                                               >  2:6057025/1‑150 (MQ=255)
                                                           gcagcaCCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCCGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAg                                                                                            >  1:6047347/1‑150 (MQ=255)
                                                           gcagcaCCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGTCGACGGGGTGCAGCGCCCGTCCGCGTACGGCGAGGGCCGCTGCGACGAAg                                                                                            >  2:3648114/1‑150 (MQ=255)
                                                             accccgtGGCCCTGCGGCCCGCGGCCGGTCGGCTGGCCGGCCCGGCGCCGGGCGGGGGGTCGGCCGCTGGCGTCAGCGTCGGCGCGCCGGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGcc                                                                                          <  2:739597/144‑1 (MQ=255)
                                                              gcaCCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCg                                                                                         >  2:2400831/1‑150 (MQ=255)
                                                               caCGTGGACGTGTTGCTCGAGGCGTGGCGGTGGGCGGGCCCTGCGCCGGGTGGGGGGTTGGTCGCGGGCTTCTGCGGCGGCGCGATTGGGGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGa                                                                                        <  2:10301448/150‑1 (MQ=255)
                                                                 ccTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCCGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGAtt                                                                                      >  2:1952429/1‑150 (MQ=255)
                                                                   tGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGCCGTCGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCg                                                                                    >  2:9910282/1‑150 (MQ=255)
                                                                   tGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCg                                                                                    >  2:8854379/1‑150 (MQ=255)
                                                                   gggACCTGCTGCACTTTGCCTGTCGGGTGGACGGCCCTGCGCCTGGCGGGGGTTTGGACGCGGGCTTCTGCGTCGTCGCGCCTGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCg                                                                                    <  2:1756395/149‑1 (MQ=255)
                                                                    ggACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGTCGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGt                                                                                   >  1:886929/1‑150 (MQ=255)
                                                                     gACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCCGACCGCGTACGGCGCGGGCCGCTGCGACGAAGCCGATTCGtt                                                                                  >  1:4176714/1‑150 (MQ=255)
                                                                       ccggctgcACGAGGCCTGTCGGGTGCACGGCCCTGCGCGGGGCGGGGGGTAGGCCGCTGGGTTCTGGGTCGGCGCGCCCGGCCCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTcc                                                                                <  2:3135717/147‑1 (MQ=255)
                                                                           ctgcACGCGGCCTGTCGGCGGGCCGGCCCTGCGCCTGGCGCGGGGTTGGACGGTGGCGTCAGCGTCGTCGCGCCTGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGc                                                                            <  2:2193486/150‑1 (MQ=255)
                                                                           ctgcACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCCGGCGCGCGACGGCGGGGAGCAGCGCCCGACCGAGTACGGCGCGGGCCGCTGCGACGAAGCCGCGTCGTTCCCGGc                                                                            >  1:6687967/1‑150 (MQ=255)
                                                                            tgcACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGTTTCGTTCCAGGcc                                                                           >  1:2559756/1‑150 (MQ=255)
                                                                            tgcACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGcc                                                                           >  1:7745707/1‑150 (MQ=255)
                                                                            tgcACGAGGCCTGGCGGCGGCACGGCCCTGCGCCGGGCGCGGGGTTGGACGCGGGCGCCCGCGTCGTCGCGCCCGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGcc                                                                           <  2:585692/150‑1 (MQ=255)
                                                                             gcACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGGCGCGCCAGGCGCGCGAAGACGGGGAGCAGCGCCCGACCGAGTACGGCGAGGGCCGGTGCGACGAAGCCGATTCGGTCCCGGCCg                                                                          >  1:5772371/1‑150 (MQ=255)
                                                                               aCGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGCCGGGGGGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCgag                                                                        >  2:6732775/1‑150 (MQ=255)
                                                                                cgttGCCTGTCGGCTGGCCGGCCCGGCGCCTGGCGGGGGGTGGGACGCGGGCGTCAGCGCCGGCGCGCCCGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCgaga                                                                       <  2:3357180/147‑1 (MQ=255)
                                                                                cgctGCGTGTCGGCGGGCGGGCCCGGGGCCGGGGGGGGGGTCGGACGCTGGCGTCTGCGTCGGCGCGCCCGCCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCgaga                                                                       <  1:3338581/147‑1 (MQ=255)
                                                                                cGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCCGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGTGGGCCGCTGCGACGGAGCCGATTCGTTCCAGGCCgaga                                                                       >  1:2352497/1‑150 (MQ=255)
                                                                                cGAGGCCTGGCGGCTGCCCGCCCCGGCGCGGGGCGGGGGGTAGGACGGTGGCGTCCGCTGCGTCGCGCCTGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCgaga                                                                       <  2:8535175/150‑1 (MQ=255)
                                                                                 gctGCCGGGCGGCGGGCCGCCCCGGCGCCTGGCGGGGGGTTGGCCGCGGCCTTCCGCTCCGCCCCCCCCGGCCCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGagag                                                                      <  1:9400619/148‑1 (MQ=255)
                                                                                       tGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAGTCCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGa                                                                >  2:4146466/1‑150 (MQ=255)
                                                                                       tGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGa                                                                >  2:3546702/1‑150 (MQ=255)
                                                                                          gggCGGGACGGCCCTGCGCGTGGGGGGGTGTTGGAGGCGGGCGTCAGCGGCGTGGCGCCTGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATgg                                                             <  2:3252273/149‑1 (MQ=255)
                                                                                          cGGGTGGACGGCCCTGCGCGTGGCGCGGGGTCGGACGCTGGCGTCAGCGTCGTCGCGCCTGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATgg                                                             <  2:10582321/150‑1 (MQ=255)
                                                                                             gtGGAGGCCCCCGCGCGTGGGGCGGGTTCGGTCGGTGGGCTATGCGTTGCCCCCCCGGCCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGtct                                                          <  1:2889547/149‑1 (MQ=255)
                                                                                              tggcgggCCCTGCGCCGGGCGGGGGGTTGGCGGGGGGCGACTGGGTGGCCGCGCCGGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGtctc                                                         <  2:4622806/145‑1 (MQ=255)
                                                                                              ggcaCGGCCCGGCGCCTGGCGGGGGGTTTGCCGGTGGCGTCAGGGTGGCCGCGCCCGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGtctc                                                         <  1:9769609/147‑1 (MQ=255)
                                                                                               ggAGGGCCCTGCGCCGGGGGGGGGGTTGGACGCGGGCGTATGCGTGGGCGCGCCTGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGtctct                                                        <  1:8215760/150‑1 (MQ=255)
                                                                                               gcaCGGCCCGGCGCCGGGCGCGGTGTCGGACGCGGGCCTCAGGGCCCCCGCGCCCGCCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGtctct                                                        <  1:10283335/148‑1 (MQ=255)
                                                                                               ctccgccccTGCCCCGGGCGCGGGGTAGGCCTCTGGCGACTGCGCCGCCGCCCCCCCCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTCCGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGtctct                                                        <  1:4722753/144‑1 (MQ=255)
                                                                                                gcgggCCCGGCGCCGGGGGGGGGTTCGGACGCTGGCGTCAGGGTCGCCGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGtctctc                                                       <  1:4501680/147‑1 (MQ=255)
                                                                                                 ccgccccGGCGCCTGGCGGGGGTTTGGACGTGGCCTTCAGCGTCGGCGCCCCTGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGTCTCTcc                                                      <  1:6158703/146‑1 (MQ=255)
                                                                                                    gCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGGGGGCCGCTGCGACGGAGCCGATTAGTTCCAGGCCGAGAGTGGCGATGGTGTGTCCGcc                                                   >  1:514824/1‑150 (MQ=255)
                                                                                                    gCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGTCTCTCCGcc                                                   >  2:8111949/1‑150 (MQ=255)
                                                                                                    gCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGGCGCGCCCGGCGCGCGTCGACGGGGGGCCGCGCCAGACCGCGTACGGCGAGGGCCCCTGCGTCGTCGCCGATTAGTTCCAGGCCGAGAGTCGGGAGTGTATCTCCGcc                                                   >  1:6540744/1‑150 (MQ=255)
                                                                                                    gCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGAGTCGTCGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGAACGGCGAGGGCCGCTGCGACGCAGCGGAGTCGTTCCAGGCCGAGAGTCGCGATGGTCTCTCCGcc                                                   >  2:6600041/1‑150 (MQ=255)
                                                                                                     ccgtGCGCCTGGCGCGGGGTTGGCCGGTGGCGTCCGCGTCGGCGCGCCTGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGTCTCTCCGCCa                                                  <  2:7643429/147‑1 (MQ=255)
                                                                                                     cccTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGTCTCTCCGccc                                                  >  1:10969814/1‑149 (MQ=255)
                                                                                                      ccTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGTCTCTCCGCCaa                                                 >  2:2679309/1‑150 (MQ=255)
                                                                                                        ggcgcCTGGCGGGGGTTCGGACGCTGGCGTCTGCGGCGTCGCGCCTGGCGCGCGCCGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGGTCCAGGCCGAGAGTCGCGATGGTCTCTCCGCCAAGt                                               <  2:474043/149‑1 (MQ=255)
                                                                                                         gcgcCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGTCTCTCCGCCAAGTa                                              <  2:1416859/150‑1 (MQ=255)
                                                                                                         gcgcCGGGCGGGGGGTTGGACGGTGGCGTCTGCGTCGACGCGCCCGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGTCTCTCCGCCAAGTa                                              <  2:9146774/150‑1 (MQ=255)
                                                                                                               ggCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGTCTCTCCGCCAAGTAgcgcgc                                        >  2:9305556/1‑150 (MQ=255)
                                                                                                                 cgggggtTAGGACGGTGGCGACAGGGTGGACGCGCCCGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGTCTCTCCGCCAAGTAGCGCGCCg                                      <  2:9374865/147‑1 (MQ=255)
                                                                                                                  gcgGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAGTCCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGTCTCTCCGCCAAGTAGCGCGCCGa                                     >  2:7941211/1‑150 (MQ=255)
                                                                                                                    gggtTAGGCCGCTGGCGCCTGCGTCGTCGCGCCCGGCCCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGTCTCTCCGCCAAGTAGCGCGCCGAgg                                   <  1:8130879/150‑1 (MQ=255)
                                                                                                                    ggggTAGGACGCTGGCGTCTGCGTCGTCGCGCCTGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGTCTCTCCGCCAAGTAGCGCGCCGAgg                                   <  2:3420424/150‑1 (MQ=255)
                                                                                                                     ggttAGGCCGCGGGCGTCTGCGTGGTCGCGCCCGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGTCTCTCCGCCAAGTAGCGCGCCGAGGc                                  <  1:7643556/147‑1 (MQ=255)
                                                                                                                      ggTTGGACGGGGGCGTCAGCGTCGCCGCGCCCGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGTCTCTCCGCCAAGTAGCGCGCCGAGGcc                                 <  2:9578098/150‑1 (MQ=255)
                                                                                                                      ggTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGAAGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGTCTCTCCGCCAAGTAGCGCGCCGAGGcc                                 >  2:5667570/1‑150 (MQ=255)
                                                                                                                       ttAGCACGCGGGCGTCAGCGACGACGCGCCCGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGTCTCTCCGCCAAGTAGCGCGCCGAGGCCg                                <  2:10839271/149‑1 (MQ=255)
                                                                                                                        ttggcggCTGGCTTCCGCGTCGTCGCGCCTGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGTCTCTCCGCCAAGTAGCGCGCCGAGGCCGa                               <  1:4052550/144‑1 (MQ=255)
                                                                                                                         tggccGCTGGGTTCAGCGTCGTCGCGCCCGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGTCTCTCCGCCAAGTAGCGCGCCGAGGCCGAt                              <  1:6205049/146‑1 (MQ=255)
                                                                                                                         tggAGGCTGGGGTCAGGGTCGTCGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGTCTCTCCGCCAAGTAGCGCGCCGAGGCCGAt                              <  2:9360205/149‑1 (MQ=255)
                                                                                                                          ggACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGTCTCTCCGCCAAGTAGCGCGCCGAGGCCGATc                             >  2:8065048/1‑150 (MQ=255)
                                                                                                                            aggCTGGCGTCAGCGTCGGCGCGCCCGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGTCTCTCCGCCAAGTAGCGCGCCGAGGCCGATccc                           <  2:8679884/148‑1 (MQ=255)
                                                                                                                            aCGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGTCTCTCCGCCAAGTAGCGCGCCGAGGCCGATccc                           <  2:776406/150‑1 (MQ=255)
                                                                                                                                tGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGTCTCTCCGCCCAGTAGCGCGCCGAGGCCGATCCCGGcc                       >  2:3931085/1‑149 (MQ=255)
                                                                                                                                    ctCAGCGTCGTCGCCCCAGGCCCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGTCTCTCCGCCAAGTAGCGCGCCGAGGCCGATCCCGGCATTgg                   <  2:4981981/149‑1 (MQ=255)
                                                                                                                                       aGCGTCGTCGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGTCGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGTCTCTCCGCCAAGTAGCGCGCCGAGGCCGATCCCGGCATTggcgg                >  2:10045945/1‑150 (MQ=255)
                                                                                                                                            cgtcgCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGTCTCTCCGCCAAGTAGCGCGCCGAGGCCGATCCCGGCATTGgcggcggcg           >  2:287609/1‑150 (MQ=255)
                                                                                                                                             gtcgCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGGTCCAGGCCGAGAGTCGCGATGGTCTCTCCGCCCAGTAGCGCGCCGAGGCCGATCCCGGCATGGgcggcggcgc          >  1:2120374/1‑149 (MQ=255)
                                                                                                                                               cgcgCCAGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCAGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCTCGATGGTCTCTACGCCAAGTAGCGCGCCGAGGCCGATCCCGGCATTGGCGGCGGCGACg        >  2:7036811/1‑150 (MQ=255)
                                                                                                                                                gcgcCAGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGTCTCTCCGCCAAGTAGCGCGCCGAGGCCGATCCCGGCATTGGCGGCGGCGACGt       >  1:1308335/1‑150 (MQ=255)
                                                                                                                                                     aGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGTCTCTCCGCCAAGTAGCGCGCCGAGGCCGATCCCGGCATTGGCGGCGGCGACGTTGCTg  >  1:1797590/1‑150 (MQ=255)
                                                                                                                                                     aGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGTCTCTCCGCCAAGTAGCGCGCCGAGGCCGATCCCGGCATTGGCGGCGGCGACGTTGCTg  >  1:6014892/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CTCTATCCGTCGCACCGGCATCCGTCGACGATCGTCAGGTGCTACCTGGATTTCTGCCAGCAGCACCTGGACCTGCTGCACGATGCCTGTCGGCTGGACGGCCCTGCGCCTGGCGCGGGGTAGGACGCTGGCGTCAGCGTCGTCGCGCCAGGCGCGCGACGACGGGGAGCAGCGCCAGACCGAGTACGGCGAGGGCCGCTGCGACGAAGCCGATTCGTTCCAGGCCGAGAGTCGCGATGGTCTCTCCGCCAAGTAGCGCGCCGAGGCCGATCCCGGCATTGGCGGCGGCGACGTTGCTG  >  NC_008463/867229‑867527

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑