Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084632,954,0030TG26.2% 868.5 / 49.3 384Q75P (CAG→CCG) PA14_RS13685DUF6162 family protein
Reads supporting (aligned to +/- strand):  ref base T (96/183);  new base G (99/0);  total (201/183)
Fisher's exact test for biased strand distribution p-value = 2.46e-36
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 390 total aligned reads displayed.

CAGGTAGCTCGGACGCCCGGCGGTTTCGCCGTGGCTCCAGCGGTGCTCGCCGCGGCTGCTCGCGGCGACGTCGGCGACGAAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCTGGATGTCTTCGGCGGCGACCTGCAGGTCGGCATAGATGCCCTGTTCGGCGGCATTCAGGTCGAGCCGGGCGTCCACCTGGTGGGCGTCCAGGGCTTGCGCTTCATGGGACTGCTGGCGCAGCCCGACCACCGTGGCGGCGAGGGCCAGG  >  NC_008463/2953855‑2954152
                                                                                                                                                    |                                                                                                                                                     
 aGGTAGCTCGGACGCCCGGCGGTTTCGCCGTGGCTCCAGCGGTGCTCGCCGCGGCTGCTCGCGGCGACGTCGGCGACGAAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTGCAGGCGCGCCTgg                                                                                                                                                     >  1:1183591/1‑150 (MQ=255)
  ggTAGCTCGGACGCCCGGCGGTTTCGCCGTGGCTCCAGCGGTGCTCGCCGCGGCTGCTCGCGGCGACGTCGGCGACGAAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCTGGa                                                                                                                                                    <  1:5507486/150‑1 (MQ=255)
  ggTAGCTCGGACGCCCGGCGGTTTCGCCGTGGCTCCAGCGGTGCTCGCCGCGGCTGCTCGCGGCGACGTCGGCGACGAAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCTGGa                                                                                                                                                    <  1:8740084/150‑1 (MQ=255)
   gTAGCTCGGACGCCCGGCGGTTTCGCCGTGGCTCCAGCGGTGCTCGCCGCGGCTGCTCGCGGCGACGTCGGCGACGAAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCTGGAt                                                                                                                                                   <  1:9542811/150‑1 (MQ=255)
     aGCTCGGACGCCCGGCGGTTTCGCCGTGGCTCCAGCGGTGCTCGCCGCGGCTGCTCGCGGCGACGTCGGCGACGAAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCTGGATgg                                                                                                                                                 >  2:6067852/1‑149 (MQ=255)
      gCTCGGACGCCCGGCGGTTTCGCCGTGGCTCCAGCGGTGCTCGCCGCGGCTGCTCGCGGCGACGTCGGCGACGAAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCTGGATGTc                                                                                                                                                <  1:5631388/150‑1 (MQ=255)
        tCGGACGCCCGGCGGTTTCGCCGTGGCTCCAGCGGTGCTCGCCGCGGCTGCTCGCGGCGACGTCGGCGACGAAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCTGGATGTCtt                                                                                                                                              <  2:7039655/150‑1 (MQ=255)
         cGGACGCCCGGCGGTTTCGCCGTGGCTCCAGCGGTGCTCGCCGCGGCTGCTCGCGGCGACGTCGGCGACGAAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCTGGATGTCTTc                                                                                                                                             <  2:1673925/150‑1 (MQ=255)
          ggACGCCCGGCGGTTTCGCCGTGGCTCCAGCGGTGCTCGCCGCGGCTGCTCGCGGCGACGTCGGCGTCGAAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCGGGATGTCTTcg                                                                                                                                            >  1:904108/1‑150 (MQ=255)
          ggACGCCCGGCGGTTTCGCCGTGGCTCCAGCGGTGCTCGCCGCGGCTGCTCGCGGCGACGTCGGCGACGAAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCTGGATGTCTTcg                                                                                                                                            <  2:7337008/150‑1 (MQ=255)
            aCGCCCGGCGGTTTCGCCGTGGCTCCAGCGGTGCTCGCCGCGGCTGCTCGCGGCGACGTCGGCGACGTAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTGCAGGCGCGGCGGGATGGGTTcggg                                                                                                                                          >  1:8397623/1‑149 (MQ=255)
            aCGCCCGGCGGTTTCGCCGTGGCTCCAGCGGTGCTCGCCGCGGCTGCTCGCGGCGACGTCGGCGACGAAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCGGGTTGTCTTcggc                                                                                                                                          >  1:1557981/1‑150 (MQ=255)
                ccGGCGGTTTCGCCGTGGCTCCAGCGGTGCTCGCCGCGGCTGCTCGCGGCGACGTCGGCGACGAAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCGGGATGTCTTCGgcggcg                                                                                                                                      >  1:5263611/1‑150 (MQ=255)
                 cggcggTTTCGCCGTGGCTCCAGCGGTGCTCGCCGCGGCTGCTCGCGGCGACGTCGGCGACGAAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCGGGATGTCTTCGgcggcgc                                                                                                                                     >  1:6907922/1‑149 (MQ=255)
                  ggcggTTTCGCCGTGGCTCCAGCGGTGCTCGCCGCGGCTGCTCGCGGCGACGTCGGCGACGAAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCTGGATGTCTTCGGCGGCGAc                                                                                                                                    <  2:5855391/150‑1 (MQ=255)
                       tttCGCCGTGGCTCCAGCGGTGCTCGCCGCGGCTGCTCGCGGCGACGTCGGCGACGAAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCTGGATGTCTTCGGCGGCGACCTGCa                                                                                                                               <  1:7722918/150‑1 (MQ=255)
                        ttCGCCGTGGCTCCAGCGGTGCTCGCCGCGGCTGCTCGCGGCGACGTCGGCGACGAAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCCGGCGCGCCTGGATGTCTTCGGCGGCGACCTGCAg                                                                                                                              >  1:8249334/1‑150 (MQ=255)
                         tCGCCGTGGCTCCAGCGGTGCTCGCCGCGGCTGCTCGCGGCGACGTCGGCGACGAAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGCGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCGGGATGTCTTCGGCGGGGACCCGCacg                                                                                                                             >  1:9275861/1‑148 (MQ=255)
                         tCGCCGTGGCTCCAGCGGTGCTCGCCGCGGCTGCTCGCGGCGACGTCGGCGACGAAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCTGGATGTCTTCGGCGGCGACCTGCAgg                                                                                                                             <  1:8853570/150‑1 (MQ=255)
                         tCGCCGTGGCTCCAGCGGTGCTCGCCGCGGCTGCTCGCGGCGACGTCGGCGACGAAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCTGGATGTCTTCGGCGGCGACCTGCAgg                                                                                                                             >  2:2945765/1‑150 (MQ=255)
                         tCGCCGTGGCTCCAGCGGTGCTCGCCGCGGCTGCTCGCGGCGACGTCGGCGACGAAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCGGGATGGCGGCGGCGGCGAccggccgg                                                                                                                             >  1:10326447/1‑150 (MQ=255)
                           gCCGTGGCTCCAGCGGTGCTCGCCGCGGCTGCTCGCGGCGACGTCGGCGACGAAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCTGGATGTCTTCGGCGGCGACCTGCAGGTc                                                                                                                           <  1:7540888/150‑1 (MQ=255)
                              gTGGCTCCAGCGGTGCTCGCCGCGGCTGCTCGCGGCGACGTCGGCGACGAAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCTGGAGGTCTTCGGCCGCGACCTGCAGGTCGGc                                                                                                                        >  2:8653309/1‑150 (MQ=255)
                               tGGCTCCAGCGGTGCTCGCCGCGGCTGCTCGCGGCGACGTCGGCGACGAAGGGCGGGAAGCCCTCGGCGGCCAGGTCGTCGACGGAGGGCGCGGCTTCGCCGGCGTCCCGGCGCGCGGGGGTGGCTTGGGCGGCGGCCCGCCGGTCgggg                                                                                                                       >  1:8698280/1‑148 (MQ=255)
                                ggCTCCAGCGGTGCTCGCCGCGGCTGCTCGCGGCGACGTCGGCGACGAAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGTCTTCGCCGTCGTCCAGGCGCGCCTGGATGTCTTCGGCGGCGACCTGCAGGTCGGCAt                                                                                                                      <  1:4378074/150‑1 (MQ=255)
                                ggCTCCAGCGGTGCTCGCCGCGGCTGCTCGCGGCGACGTCGGCGACGAAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTGCAGGCGCGCCGGGCTGTGGTCGGCGGCGGCCGGCCGGTCgggag                                                                                                                      >  1:1097256/1‑147 (MQ=255)
                                 gcaccAGCGGTGCTCGCCGCGGCTGCTCGCGGCGACGTCGGCGACGAAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGCCGGAGCGCGCGGCTTCGCCCTCGTCCAGGCGCGCCTGGCGGACTTCGGCGGCGACCTGCAGGTCGGCATa                                                                                                                     <  2:10536908/147‑1 (MQ=255)
                                  cgccAGCGGTGCTCGCCGCGGCTGCTCGCGGCGACGTCGGCGACGAAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTGCCGGCGCGCCGGGGGGTGGTGGGCGGCGGCCggcggggcgggcgag                                                                                                                    >  1:8508688/3‑145 (MQ=255)
                                  cTCCAGCGGTGCTCGCCGCGGCTGCTCGCGGCGACGTCGGCGACGAAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCTGGGTGTGTTAGGCGGCGCCCTGCAGGTGGGCATAg                                                                                                                    >  1:6818254/1‑150 (MQ=255)
                                   tCCAGCGGTGCTCGCCGCGGCTGCTCGCGGCGACGTCGGCGACGAAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCTGGATGTCTTCGGCGGCGACCTGCAGGTCGGCATAGa                                                                                                                   <  1:6363672/150‑1 (MQ=255)
                                       gCGGTGCTCGCCGCGGCTGCTCGCGGCGACGTCGGCGACGAAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTGCAGGGGCGCGGGGATGTGTTAGGGGGCGACCTGGGGGGCGGCCTAGATggc                                                                                                               >  2:147293/1‑148 (MQ=255)
                                       gCGGTGCTCGCCGCGGCTGCTCGCGGCGACGTCGGCGACGAAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCTGGCTGTGGTCGGCGGCGACCAGCAGGGCGGCGTAGATGcc                                                                                                               >  2:6669064/1‑150 (MQ=255)
                                       gCGGTGCTCGCCGCGGCTGCTCGCGGCGACGTCGGCGACGAAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCAGGATGTGTTCGGCGGCGCCCTGCAGGTCGGCCTAGATGcc                                                                                                               >  2:10374240/1‑150 (MQ=255)
                                            gCTCGCCGCGGCTGCTCGCGGCGACGTCGGCGACGAAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCTGGATGGCTTCGGCGGCGACCTGCAGGTCGGCATAGATGCCCTGtt                                                                                                          <  2:4153613/150‑1 (MQ=255)
                                             cTCGCCGCGGCTGCTCGCGGCGACGTCGGCGACGAAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCTGGATGTCTTCGGCGGCGACCAGCAGGTCGGCCTAGATGCCCTGtta                                                                                                         >  2:4758301/1‑149 (MQ=255)
                                              tCGCCGCGGCTGCTCGCGGCGACGTCGGCGACGAAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCGGGAGGTCGTCGGCGGCGTCCTGCAGGGCGGCCTAGATGACCTGTTcg                                                                                                        >  1:4529154/1‑150 (MQ=255)
                                               cgccgcGGCAGCTCGCGGCGACGTCGGCGACGAAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGTCGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCTGGATGTCTTCGGCGGCGCCCTGCAGGTCGGCATAGATGCCCTGTTcgg                                                                                                       <  2:9611661/150‑1 (MQ=255)
                                                    cgGCTGCTCGCGGCGACGTCGGCGACGAAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCTGGGGGTCGTGGGCGGCGGCCGGCAGGTCGGCGGAGTTGACCGGGTCggcggcg                                                                                                  >  1:1883337/1‑149 (MQ=255)
                                                    cgGCTGCTCGCGGCGACGTCGGCGACGAAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCTGGATGTCTTCGGCGGCGACCTGCAGGTCGGCATAGATGCCCTGTTCGGCGGCa                                                                                                  <  2:1498305/150‑1 (MQ=255)
                                                     ggCTGCTCGCGGCGACGTCGGCGACGAAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCGGGATGTCGTCGGCGGCGGCCTGCAGGTCGGCATAGATGCCCTGTTCggcggcct                                                                                                 >  2:3057513/1‑148 (MQ=255)
                                                      gctgctCGCGGCGACGTCGGCGACGACGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCGGGAGGTCTTGGGCGGCGACCTGCAGGTCGGCAGAGATGCCCTGTTCggcggcgat                                                                                                >  2:1339895/1‑147 (MQ=255)
                                                      gctgctCGCGGCGACGTCGGCGACGAAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCTGGATGTCTTCGGCGGCGACCTGCAGGTCGGCATAGATGCCCTGTTCGGCGGCAtt                                                                                                <  2:8973633/150‑1 (MQ=255)
                                                      gctgctCGCGGCGACGTCGGCGACGAAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCGGGATGTGGTCGGCGGCGGCCTGCCGGTCGGCATAGATGGCCGGTTCGGCGGCaat                                                                                                >  2:2275962/1‑148 (MQ=255)
                                                          ctCGCGGCGACGTCGGCGACGAAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGGGCGCCTGGATGTGTTCGGGGGCGACCGGCACGTCGGCATAGATGCCCGGTTCGGCGGCGTTAAgg                                                                                            >  1:6668300/1‑150 (MQ=255)
                                                          ctCGCGGCGACGTCGGCGACGAAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCTGGATGTCTTCGGCGGCGACCTGCAGGTCGGCATAGATGCCCTGTTCGGCGGCATTCAgg                                                                                            <  1:6224185/150‑1 (MQ=255)
                                                           tCGCGGCGACGTCGGCGACGAAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCTGGAGGTCTTCGGCGGCGACCTGCAGGTCGGCATAGATGCCCTGTTCGGCGGCATTCAGGt                                                                                           <  1:4149192/150‑1 (MQ=255)
                                                           tCGCGGCGACGTCGGCGACGAAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCAGGAGGTAGTCGGCGGCGACCGGCAGGGCGGCATAGATGCCCCGTGCGGCGGCATTAAggg                                                                                           >  1:10200443/1‑149 (MQ=255)
                                                                 cgACGTCGGCGACGAAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCTGGATGTCTTCGGCGGCGACCTGCAGGTCGGCATAGATGCCCTGTTAGGCGGCCTTCAGGTCGAGcc                                                                                     >  2:9307440/1‑150 (MQ=255)
                                                                   aCGTCGGCGACGAAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCAGGCTGTCTTCGGCGGCGACCTGCAGGTCGGCCTATATGCCCTGTTAGGCGGCATTCAGGTCGAGCCgg                                                                                   >  2:6515185/1‑150 (MQ=255)
                                                                    cGTCGGCGACGAAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCGGGATGGCTTCGGCGGCGCCCTGCAGGTCGGCCTAGATGGCCGGTTCGGCGGCATTCAGGTCGAGCCggg                                                                                  >  1:9083468/1‑150 (MQ=255)
                                                                     gTCGGCGACGAAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCGGGGTGTCTTCGGCGGCGACCTGCAGGGCGGCAGAGATGCCCGGTGCGGCGGCCTTCAGGGCGAGCCGGGc                                                                                 >  1:9143291/1‑150 (MQ=255)
                                                                      tCGGCGACGAAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCCGGCGCGCGTGGGAGTCTTCGGCGGCGACCTGCCGGTCGGCCGTGAAGCCCGGTTCGGCGTCCTTCACGTCCAggcgggcg                                                                                >  2:10252821/1‑150 (MQ=255)
                                                                      tCGGCGACGAAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCTGGATGTCTTCGGCGGCGACCTGCAGGTCGGCATAGATGCCCTGTTCGGCGGCATTCAGGTCGAGCCGTGCg                                                                                <  2:7504511/150‑1 (MQ=255)
                                                                        ggCGACGAAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCTGGATGTCTTCGGCGGCGACCAGCAGGTCGGCATAGATGCCCTGTTCGGCGGCAATCAGGTCGAGCCGGGCGTc                                                                              >  2:7183404/1‑150 (MQ=255)
                                                                         gCGACGAAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCTGGATGTGTTGGTCGGCGACCAGCCGGTCGGCGTAGATTCCCAGGTAGGCGGCCTTGAGGTCGAGCCGGGCGTcc                                                                             >  1:2373422/1‑150 (MQ=255)
                                                                         gCGACGAAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCTGGATGTCTTCGGCGGCGACCTGCAGGTCGGCATAGATGCCCTGTTCGGCGGCATTCAGGTCGAGCCGGGCGTcc                                                                             <  2:2004218/150‑1 (MQ=255)
                                                                          cgacgaAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCCGGCGCGCCGGGATGTCTTCGGCGGCGACCAGCAGGTCGGCCTATATGGCCGGTTAGGGGGCATTGAGGTCGAGCCGGGCGGccc                                                                            >  1:9382231/1‑149 (MQ=255)
                                                                            acgaAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCGGGAGGTCTTCGGCGGCGGCCTGCAGGTCGGCATAGATGCCCTGTGAGGCGGCAGTCAGGTAGAGCCGGGCGTCCAcc                                                                          >  1:10058579/1‑150 (MQ=255)
                                                                             cgaAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCGGGATGGCGTCGGCGGCGACCGGCAGGTCGGCATATATGACCAGGTAGGCGGCATTCAGGTCGAGCCGGGCGTCCACCt                                                                         >  1:7991796/1‑150 (MQ=255)
                                                                              gaAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCTGGATGTCTTCGGCGGCGACCTGCAGGTCGGCATAGATGCCCTGTTCGGCGGCATTCAGGTCGAGCCGGGCGTCCACCtg                                                                        <  1:2711210/150‑1 (MQ=255)
                                                                              gaAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCGGGATGTCTTCGGCGGCGACCTGCAGGTCGGCATAGATGACCTGTTCGGCGGGATTCAGGTCGAGCCGGGCGTCCACCtg                                                                        >  2:7768754/1‑150 (MQ=255)
                                                                                   gCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCTGGATGTCTTCGGCGGCGACCTGCAGGTCGGCATAGATGCCCTGTTCGGCGGCATTCAGGTCGAGCCGGGCGTCCACCTGGTggg                                                                   <  2:3246369/150‑1 (MQ=255)
                                                                                      ggAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCTGGATGTCTTCGGCGGCGACCTGCAGGTCGGCATAGATTCCCTGTTCGGCGGCATTCAGGTAGAGCCGGGCGTCCACCAGGTGGGCGt                                                                >  2:4338649/1‑150 (MQ=255)
                                                                                      ggAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCTGGATGTCGGCGGCGGCGCCCTGCAGGTCGGCATAGATGCCCTGTTCGGCGGCATTCAGGGCGAGCCGGGCGTGCACCTGGTGGGCgg                                                                >  2:5082209/1‑149 (MQ=255)
                                                                                       gAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCTGGATGTCTTCGGCGGCGACCTGCAGGTCGGCATAGATGCCCTGTTCGGCGGCATTCAGGTCGAGCCGGGCGTCCACCTGGTGGGCGTc                                                               <  1:2996099/150‑1 (MQ=255)
                                                                                       gAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCTGGATGTCTTCGGCGGCGACCTGCAGGTCGGCATAGATGCCCTGTTCGGCGGCATTCAGGTCGAGCCGGGCGTCCACCTGGTGGGCGTc                                                               <  1:5726908/150‑1 (MQ=255)
                                                                                            ccTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCCGGCGCCCCTGGTTGTCTTCGGCGGCCACCTCCAGGACGGCATAGATGCCCAGATCGGCGGCATTCATGACGAGCCGCGCGTACACCTGGTGGGCGTCCAggg                                                          >  1:7301211/1‑150 (MQ=255)
                                                                                             cTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCTGGGTGTCTGCGGCGGCGGCCAGCAGGGCGGCATCGAAGACCAGGTCGGGGGCAGTCAGGTCGAGCCGGGGGGCCACCAGGTGGGCGTCCAgggg                                                         >  1:10503049/1‑149 (MQ=255)
                                                                                              tCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCTGGATGTCTTCGGCGGCGACCTGCAGGTCGGCATAGATGCCCTGTTCGGCGGCATTCAGGTCGAGCCGGGCGTCCACCTGGTGGGCGTCCAGGGCt                                                        <  2:8494595/150‑1 (MQ=255)
                                                                                               cggcggCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCCGGCGCGCCGGGATGTCTTCGGCGGCGACCAGCCGGTCGGCCTAGATGCCCTGTTAGGCGGCAATCAGGTCGAGCCGGGCGTCCACCGGGTGGGCGTCCAGGGCtg                                                       >  2:5650950/1‑149 (MQ=255)
                                                                                                 gcggcCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCTGGGTGTCTTCGGCGGCGCCCTGCCGGTCGGCCTAGATGACCAGGTCGGCGGCCTTCAGGTCGAGCCGGGCGTCCACCAGGGGGGCGTGCCGGGcgggg                                                     >  1:7030129/1‑146 (MQ=255)
                                                                                                    gcCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCTGGATGTCTTCGGCGGCGACCTGCAGGTCGGCATAGATGCCCTGTTCGGCGGCATTCAGGTCGAGCCGGGCGTCCACCTGGTGGGCGTCCAGGGCTTGCGCt                                                  <  1:5915283/150‑1 (MQ=255)
                                                                                                     ccAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCGGGTTGTCTTCGGCGGCGACCTGCAGGTCGGCATAGATGCCCTGTTCGGCGGCATTCAGGTCGAGCCGGGCGTCCACCTGGTGGGCGTCCCGGGCTTGCGCtt                                                 >  1:2552247/1‑150 (MQ=255)
                                                                                                       aGTGCGTCGACGGAGGGCGCGGCTTCGCCGGCGTGCAGGCGCCCCGGGATGTGTTTGGCGGCGACCAGCAGGTAGGCATAGATGCCCTGTTCGGCGGCATTCAGGTCCAGCCGGGCGTCCACCTGGTGGGCGGCCCGGGCGTGCGCTTcg                                               >  2:10819744/1‑149 (MQ=255)
                                                                                                        gTTCGTCGACGGAGGGCGCGGCTTCTCCGTCGTCCAGGCGCGCCTGGCAGTCTTCCGCGGCGCCCTGCAGGTCGGCATAAATGCCCTGTTAGGCCGCAGTCAGTTCAAGCCCGGAGGCACCCGGGGGGGGGTGCAGGGCGGgcgcatgat                                              >  2:1156155/1‑145 (MQ=255)
                                                                                                           cgtcgACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCTGGATGTCTTCGGCGGCGACCTGCAGGTCGGCATAGATGCCCTGTTCGGCGGCATTCAGGTCGAGCCGGGCGTCCACCTGGTGGGCGTCCAGGGCTTGCGCTTCATggg                                           <  1:3480529/150‑1 (MQ=255)
                                                                                                               gACGGAGGGCGCGGCTTCGCCGTCGTCCAGCCGCGCCTGGAAGTCTTCGGCGGCGGCCGGCAGGTCGGCATAGATGCCCAGTTCGGCGGCATTCAGGTCGCGCCGGGCGTCCACCGGGTGGGCGTCCAGGGCTTGCGCTACATGGGActg                                       >  2:6808385/1‑150 (MQ=255)
                                                                                                                aCGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCTGGATGTCTTCGGCGGCGACCTGCAGGTCGGCATAGATGCCCTGTTCGGCGGCATTCAGGTCGAGCCGGGCGTCCACCTGGTGGGCGTCCAGGGCTTGCGCTTCATGGGActgc                                      <  1:142178/150‑1 (MQ=255)
                                                                                                                   gAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCTGGATGTCTTCGGCGGCGACCTGCAGGTCGGCATAGATGCCCTGTTCGGCGGCATTCAGGTCGAGCCGGGCGTCCACCTGGTGGGCGTCCAGGGCTTGCGCTTCATGGGACTGCTgg                                   <  1:2139408/150‑1 (MQ=255)
                                                                                                                    aGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCTGGATGTCTTCGGCGGCGACCTGCAGGTCGGCATAGATGCCCTGTTCGGCGGCAGTCAGGTCGAGCCGGGCGTCCACCTGGTGGGCGTCCAGGGCTTGCGCGTCATGGGACTGCTGgc                                  >  2:3445744/1‑150 (MQ=255)
                                                                                                                     gggCGCGGCTTCGCCGTCGTCCAGGCGCGGCTGGCTGGCTTCGGCGGCGACCTGCAGGGCGGGATAGATGCCCTGTTCGGCGGGAGTCAGGTCGAGGCGGGCGGCCCCCTGGTGGGCGTCCAGGGCTTGCGCTGCTTGGGACTGCTgggg                                 >  2:10007373/1‑148 (MQ=255)
                                                                                                                         gcgGCTTCGCCGTCGTCCAGGCGCGCCTGGATGTCTTCGGCGGCGACCTGCAGGTCGGCATAGATGCCCTGTTCGGCGGCATTCAGGTCGAGCCGGGCGTCCACCTGGTGGGCGTCCAGGGCTTGCGCTTCATGGGACTGCTGGCGCAGc                             <  1:39901/150‑1 (MQ=255)
                                                                                                                          cgGCTTCGCCGTCGTCCAGGCGCGCCGGGATGTCTTCGGCGGCGACCAGCAGGTCGGCATAGATGCCCTGTTCGGCGGCCGTCAGGTCGAGCCGGGCGTCCACCAGGTGGGCGTCCAGGGCTTGCGCTTCATGGGACTTCTGGCGCAGcc                            >  1:10001087/1‑150 (MQ=255)
                                                                                                                           ggCTTCGCCGTCGTCCAGGCGCGCCTGGATGTCTTCGGCGGCGGCCGGCAGGTCGGCAAAGATGCCCTGTTCGGCGGCATTCAGGTCGAGGCGGGCGTCCACCAGGTGGGCGTCCAGGGCGTGCGCTTCATGGGACTGCTGGCGCCGccc                           >  2:7325255/1‑150 (MQ=255)
                                                                                                                            gCTTCGCCGTCGTCCAGGCGCGCCTGGAGGTCTTCGGCGGCGACCAGCAGGTCGGCATAGAGGCCCTGTTCGGCGGCATTCAGGTCGAGCCGGGCGTCCACCTGGTGGGCGTCCAGGGCTTGCGCTTCATGGGACTGCTGGCGCAGCCCg                          <  1:10799746/150‑1 (MQ=255)
                                                                                                                              ttCGCCGTCGTCCCGGCGCGCCTGGATGTCTTAGGCGGCGACCAGCAGGTCGGCATAGATGCCCGGTTCGGCGGCCTTCAGGTCGAGCCGGGCGTCCACCTGGTGGGCGTCCCGGGCTTGCGCTTCAAGGGACAGCAGGCGCAGCCCGac                        >  2:4622531/1‑150 (MQ=255)
                                                                                                                               tCGCCGTCGTCCAGGCGCGCCGGGATGTCTGCGGCGGCGACCTGCCGGTCGGCATAGGTGCCCTGGGGGGCGGCCTTCCGGTCGTGCCGGGCGTCCACCAGGGGGGCGTCCAGGGCGTGCGCGGCATGGGACTGCGGGGGCAGGCCGacc                       >  2:82351/1‑150 (MQ=255)
                                                                                                                                  cccgcggccAGGCGCGCCTGGCTGTCGGCGGCGGCGACCTGCAGGTCGGCATAGATGCCCTGTTCGGCGGCAGTCAGGTCGAGCCGGGCGTCCACCGGGGGGGCGTCCAGGGCTTGCGCTTCATGGGACTGCTGGCGCAGCCCGaccacc                    <  2:8871767/143‑1 (MQ=255)
                                                                                                                                   cgtcgtCCAGGCGCGCCTGGATGTCTTCGGCGGCGACCTGCAGGTAGGCATAGATGCCCTGTTCGGCGGCATTCAGGTCGAGCCGGGCGTCCACCAGGTGGGCGTCCAGGGCTTGCGCTTCATGGGACTGCTGGCGCAGCCCGACCACCg                   >  2:9765597/1‑150 (MQ=255)
                                                                                                                                   cgtcgtCCAGGCGCGCCTGGATGTCTGCGGCGGCGACCTGCAGGTCGGCATAGATGCCCTGTTCGGCGGCATTCAGGTCGAGCCGGGCGTCCACCTGGTGGGCGTCCAGGGCTTGCGCTTCATGGGACTGCTGGCGCAGCCCGACCACCg                   <  1:7628192/150‑1 (MQ=255)
                                                                                                                                   cgtcgtCCAGGCGCGCCGGGATGTCTTCGGCGGCGACCTGCAGGTCGGCATAGATGCCCTGTTCGGCGGCAATCAGGTCGAGCCGGGCGTCCACCTGGTGGGCGTCCAGGGCTTGCGCTTCATGGGACTGCTGGCGCAGCCCGACCACCg                   >  2:1831158/1‑150 (MQ=255)
                                                                                                                                     tcgtcCAGGCGCGCCTGGATGTCTTCGGCGGCGACCTGCAGGTCGGCATAGATGCCCTGTTCGGCGGCATTCAGGTCGAGCCGGGCGTCCACCTGGTGGGCGTCCCGGGCTGGCGCTTCATGGGACTGCTGGCGCAGCCCGACCACCGTg                 >  2:942641/1‑150 (MQ=255)
                                                                                                                                          cAGGCGCGCCTGGATGTCTTCGGCGGCGACCTGCAGGTCGGCATATATGCCCTGTTCGGCGGCATTCAGGTCGAGCCGGGCGTCCACCTGGTGGGCGTCCAGGGCTTGCGCTTCATGGGACTGCTGGCGCAGCCCGACCACCGTggcggc            >  1:374356/1‑150 (MQ=255)
                                                                                                                                           aGGCGCGCCTGGATGTCTTCGGCGGCGACCTGCAGGTCGGCATAGATGCCCTGTTCGGCGGCATTCAGGTCGAGCCGGGCGTCCACCTGGTGGGCGTCCAGGGCTTGCGCTTCATGGGACTGCTGGCGCAGCCCGACCACCGTGgcggcg           <  1:6061744/150‑1 (MQ=255)
                                                                                                                                            ggCGCGCCTGGATGTATTCGGCGGAGACCTGCAGGACGGCATAGATGCCCAGTTCGGCGGCATTCAGTTCGAGCCGGGCGTCCACCTGGTGGGCGTCCAGGGCTTGCGCTGCATGGGACTGCTGGCGCAGCACGACCACCGTGGCGGCGa          >  2:5492080/1‑150 (MQ=255)
                                                                                                                                               gcgcCTGGATGTCTTCGGCGGCGACCTGCAGGTCGGCATAGATGCCCTGTTCGGCGGCATTCAGGTCGAGCCGGGCGTCCACCTGGTGGGCGTCCAGGGCTTGCGCTTCATGGGACTGCTGGCGCAGCCCGACCACCGTGGCGGCGAggg       <  1:3179069/150‑1 (MQ=255)
                                                                                                                                                 gcCTGGATGTCTTCGGCGGCGACCTGCAGGTCGGCATAGAGGCCCTGTTCGGCGGCATTCAGGTCGAGCCGGGCGTCCACCTGGTGGGCGTCCAGGGCTTGCGCTTCATGGGACTGCTGGCGCAGCCCGACCACCGTGGCGGCGAGGGcc     <  2:857347/150‑1 (MQ=255)
                                                                                                                                                   cTGGATGTCTTCGGCGGCGACCTGCAGGTCGGCATAGATGCCCTGTTCGGCGGCATTCAGGTCGAGCCGGGCGTCCACCTGGTGGGCGTCCAGGGCTTGCGCTTCATGGGACTGCTGGCGCAGCCCGACCACCGTGGCGGCGAGGGCCAg   >  2:5176191/1‑150 (MQ=255)
                                                                                                                                                    tGGATGTCTTCGGCGGCGACCTGCAGGTCGGCATAGATGCCCTGTTCGGCGGCATTCAGGTCGAGCCGGGCGTCCACCTGGTGGGCGTCCAGGGCTTGCGCTTCATGGGACTGCTGGCGCAGCCCGACCACCGTGGCGGCGAGGGCCAgg  >  2:8006193/1‑150 (MQ=255)
                                                                                                                                                    tGGATGTCTTCGGCGGCGACCTGCAGGTCGGCATAGATGCCCTGTTCGGCGGCATTCAGGTCGAGCCGGGCGTCCACCTGGTGGGCGTCCAGGGCTTGCGCTTCATGGGACTGCTGGCGCAGCCCGACCACCGTGGCGGCGAGGGCCAgg  >  1:4817701/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CAGGTAGCTCGGACGCCCGGCGGTTTCGCCGTGGCTCCAGCGGTGCTCGCCGCGGCTGCTCGCGGCGACGTCGGCGACGAAGGGCGGGAAGCCCTCGGCGGCCAGTTCGTCGACGGAGGGCGCGGCTTCGCCGTCGTCCAGGCGCGCCTGGATGTCTTCGGCGGCGACCTGCAGGTCGGCATAGATGCCCTGTTCGGCGGCATTCAGGTCGAGCCGGGCGTCCACCTGGTGGGCGTCCAGGGCTTGCGCTTCATGGGACTGCTGGCGCAGCCCGACCACCGTGGCGGCGAGGGCCAGG  >  NC_008463/2953855‑2954152

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑