Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084634,616,4500TG33.9% 245.4 / 18.9 157intergenic (‑170/+32)hda/PA14_RS21180DnaA regulatory inactivator Hda/DUF2066 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base T (40/46);  new base G (39/5);  total (104/53)
Fisher's exact test for biased strand distribution p-value = 1.63e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 163 total aligned reads displayed.

CGAAAACGCCGCAGGAGTCACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGC‑GCCGG‑CC‑C‑GC‑T‑CCGTTGCATAGGAGCGGATCGGCGCGCCGACTCACTGCCAGCGGAAATTCA‑GTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCAACTGCGCCAGG  >  NC_008463/4616301‑4616599
                                                                                                                                                          |                                                                                                                                                       
cGAAAACGCCGCAGGAGTCACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGC‑GCCGG‑CC‑C‑GC‑t                                                                                                                                                         <  1:2538308/150‑1 (MQ=255)
 gAAAACGCCGCAGGAGTCACCGTAAAGGCTGCAAAGTTTATCCGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGC‑GCCGG‑CC‑C‑GC‑T‑c                                                                                                                                                       <  2:3505065/150‑1 (MQ=255)
   aaaCGCCGCAGGAGTCACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGC‑GCCGG‑CC‑C‑GC‑T‑CCg                                                                                                                                                     <  2:7716388/150‑1 (MQ=255)
    aaCGCCGCAGGAGTCACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGC‑GCCGG‑CC‑C‑GC‑T‑CCGt                                                                                                                                                    <  1:5841188/150‑1 (MQ=255)
    aaCGCCGCAGGAGTCACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGC‑GCCGG‑CC‑C‑GC‑T‑CCGt                                                                                                                                                    <  1:4280314/150‑1 (MQ=255)
      cgccgcAGGAGTCACCGTAATGGCTTCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGC‑GCCGG‑CC‑C‑GC‑T‑CCGTTg                                                                                                                                                  <  1:5172100/150‑1 (MQ=255)
       gccgcAGGAGTCACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGG‑GCCGG‑CC‑C‑CG‑T‑CCGCTGc                                                                                                                                                 >  2:6347056/1‑150 (MQ=255)
       gccgcAGGAGTCACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGC‑GCCGG‑CC‑C‑GC‑T‑CCGTTGc                                                                                                                                                 <  1:5749650/150‑1 (MQ=255)
       gccgcAGGAGTCACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGC‑GCCGG‑CC‑C‑GC‑T‑CCCTTGc                                                                                                                                                 >  2:6265551/1‑150 (MQ=255)
        ccgcAGGAGTCACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCACAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGC‑GCCGG‑CC‑C‑GC‑T‑CCGTTGCa                                                                                                                                                <  1:5217032/150‑1 (MQ=255)
        ccgcAGGAGTCACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGC‑GCCGG‑CC‑C‑GC‑T‑CCGTTGCa                                                                                                                                                <  1:10772668/150‑1 (MQ=255)
        ccgcAGGAGTCACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGC‑GCCGG‑CC‑C‑GC‑T‑CCGTTGCa                                                                                                                                                <  2:9441983/150‑1 (MQ=255)
        ccgcAGGAGTCACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGC‑GCCCG‑CC‑C‑GC‑A‑CCGtttga                                                                                                                                                >  2:7947110/1‑147 (MQ=255)
        ccgcAGGAGTCACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGC‑GCCCG‑CC‑C‑CC‑C‑CCGGTGCa                                                                                                                                                >  2:7412205/1‑150 (MQ=255)
         cgcAGGAGTCACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGC‑GCCGG‑CC‑C‑GG‑T‑CCGGAGCAt                                                                                                                                               >  2:6901198/1‑150 (MQ=255)
           cAGGAGTCACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGC‑GCCGG‑CC‑C‑GC‑T‑CCGTTGCATAg                                                                                                                                             <  2:5008255/150‑1 (MQ=255)
           cAGGAGTCACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGC‑GCCGG‑CC‑C‑GC‑G‑CCGGTgggtag                                                                                                                                             >  2:7828917/1‑145 (MQ=255)
            aGGAGTCACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGC‑GCCGG‑CC‑C‑CC‑G‑CCGTTggagatg                                                                                                                                            >  2:568355/1‑144 (MQ=255)
             ggAGTCACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGC‑GCCGG‑CC‑C‑GC‑T‑CCGTTGCATAGGa                                                                                                                                           <  1:6861750/150‑1 (MQ=255)
              gAGTCACCGTAAAGGCTGCAAAGTTTATCCGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGC‑GCCGG‑CC‑C‑GC‑T‑CCGTTGCATAGGAg                                                                                                                                          <  1:8558654/150‑1 (MQ=255)
              gAGTCACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGC‑GCCGG‑CC‑C‑GC‑T‑CCGTTGCATAGGAg                                                                                                                                          <  2:5291898/150‑1 (MQ=255)
               aGTCACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGC‑GCCGG‑CC‑C‑GC‑T‑CCGTTGCATAGGAGc                                                                                                                                         <  2:1533648/150‑1 (MQ=255)
               aGTCACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGC‑GCCGG‑CC‑C‑GC‑T‑CCGTTGCATAGGAGc                                                                                                                                         <  1:6184135/150‑1 (MQ=255)
                gTCACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGG‑GCCGG‑CC‑C‑CC‑G‑CCGGTGGCTAggcggg                                                                                                                                        >  2:140713/1‑146 (MQ=255)
                gTCACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGC‑GCCGG‑CC‑C‑GC‑T‑CCGTTGCATAGGAGCg                                                                                                                                        <  2:10871856/150‑1 (MQ=255)
                 tCACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGC‑GCCGG‑CC‑C‑CC‑G‑CCGGTGCATATGAGCgg                                                                                                                                       >  2:4279087/1‑150 (MQ=255)
                  cACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGC‑GCCGG‑CC‑C‑GC‑T‑CCGTTGCATAGGAGCGGa                                                                                                                                      <  1:3840986/150‑1 (MQ=255)
                  cACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGC‑GCCCG‑CC‑C‑GC‑G‑CCGGTGCCAAGGAGCggg                                                                                                                                      >  2:8758668/1‑149 (MQ=255)
                   aCCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGC‑GCCGG‑CC‑C‑GC‑T‑CCGTTGCATAGGAGCGGAt                                                                                                                                     <  1:6145395/150‑1 (MQ=255)
                   aCCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGC‑GCCGG‑CC‑C‑GC‑T‑CCGTTGCATAGGAGCGGAt                                                                                                                                     <  1:9351170/150‑1 (MQ=255)
                   aCCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGC‑GCCGG‑CC‑C‑GC‑T‑CCGTTGCATAGGAGCGGAt                                                                                                                                     <  1:9351195/150‑1 (MQ=255)
                   aCCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGC‑GCCGG‑CC‑C‑GC‑A‑CCGGTGCAATGGAGCGGag                                                                                                                                     >  2:5748005/1‑149 (MQ=255)
                   aCCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGC‑GCCGG‑CC‑C‑CC‑G‑CCCTTGCATAGGAGCggct                                                                                                                                     >  2:9162479/1‑148 (MQ=255)
                   aCCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGC‑GCCCG‑GC‑C‑GC‑G‑CCCGTGCAAAGGAGGGGag                                                                                                                                     >  2:4643376/1‑149 (MQ=255)
                    ccGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCTCAATGAGCTACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGC‑GCCGG‑CC‑C‑GC‑T‑CCGTTGCATAGGAGCGGATc                                                                                                                                    <  2:8178432/150‑1 (MQ=255)
                    ccGTAAAGGCTGCAAAGCTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGC‑GCCGG‑CC‑C‑GC‑T‑CCGTTGCATAGGAGCGGATc                                                                                                                                    <  2:7051752/150‑1 (MQ=255)
                      gTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACATTGAGCAACTAGGCAAGCAAAATCAAAGGTGTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGC‑GCCGG‑CC‑C‑CC‑G‑CCGGTGCATACGAGCCGATggg                                                                                                                                  >  2:3502706/1‑150 (MQ=255)
                      gTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGC‑GCCGG‑CC‑C‑GG‑C‑CCGGTTCTTAGGAGCGGagccg                                                                                                                                  >  2:9570785/1‑146 (MQ=255)
                        aaaGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGC‑GCCCG‑CC‑C‑CC‑A‑CCCGTTCATAGGAGCGGAtgcgcg                                                                                                                                >  2:2677537/1‑145 (MQ=255)
                         aaGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGC‑GCCGG‑CC‑C‑GC‑A‑CCCGGTCATAGGAGCGGAAGGgcgc                                                                                                                               >  1:10760737/1‑150 (MQ=255)
                             cTGCAAAGTTTATACGCCTCGGCACGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCAATAGCTTCGGCCGGTCGCGC‑GCCGG‑CC‑C‑GC‑T‑CCGTTGCATAGGAGCGGATCGGCGCGCCg                                                                                                                           <  1:9376348/150‑1 (MQ=255)
                                  aaGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGG‑GCCGG‑CC‑C‑CC‑A‑CCCGTGCCGTGGATCGGATCGGGGCGcccccaaa                                                                                                                      >  1:818218/1‑144 (MQ=255)
                                  aaGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGC‑GCCGG‑CC‑C‑CC‑T‑CCGGTTCCAAGGAGCGGGTCGGCGCGCCCGCTCa                                                                                                                      >  2:1246147/1‑150 (MQ=255)
                                   aGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGC‑GCCCG‑CC‑C‑CC‑G‑CCCGTGAAGAGGAGCGGAGAGGCGCGCCCacacac                                                                                                                     >  2:5123151/1‑150 (MQ=255)
                                     tttATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGC‑GGCGG‑CC‑C‑GC‑A‑CCGGTTGATATTAGCGGGGCGGGGCGCCGGCacacag                                                                                                                   >  1:1193207/1‑148 (MQ=255)
                                     tttATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGC‑GCCGG‑CC‑C‑GC‑G‑CCGGGTGATAGGGGCGGGTCGGCGCGCCGCCacacag                                                                                                                   >  1:6133116/1‑148 (MQ=255)
                                      ttATACGCCTCGGCAAGGCCGCAAAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCAGATATCCGAGTCTCCTTTCCCAGAGCATCGGCCGATCGAGG‑GACGC‑CC‑C‑AC‑A‑CAGGTTGATAGGAGCGGAACGGAGCGCCGACACCCTGc                                                                                                                  >  2:9747393/1‑150 (MQ=255)
                                            gCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGG‑GCCGG‑CC‑CGGG‑C‑CCGTTGC‑TAGGGGGGGGTGGGCCCGCCCACGCACTGCCAGccg                                                                                                            >  1:11017950/1‑148 (MQ=255)
                                                 ggCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGC‑GCCCG‑CC‑C‑GC‑G‑CCGGTGGATAGGAGCGGGTCGGCGCGCCGCCTCACTGCCACCGGAAAtt                                                                                                       >  2:8622699/1‑150 (MQ=255)
                                                   cAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGG‑GCCCG‑CC‑C‑CC‑G‑CCGGTTGAAAGGGTCGGGTGGGGGCGCCGACACCATGCCCGCGGaaaatta                                                                                                     >  1:8126774/1‑146 (MQ=255)
                                                     aGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGC‑GCCGG‑CC‑C‑GC‑G‑CCGGTGCCAAGGAGCGGATCGGCGCGCCGCCACACAGCCACCGGGAAATAA‑Gt                                                                                                  >  2:10425163/1‑150 (MQ=255)
                                                       gCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGC‑GCCGG‑CC‑C‑GC‑T‑CCGGTGCATAGGTGCGGATCGGGGCGCCGACGCACTGCCCGCGGGAATAAA‑GTTg                                                                                                >  2:9124128/1‑150 (MQ=255)
                                                          gCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGG‑GCCGG‑CC‑C‑CC‑G‑CCGTTGCATAGGAGCGGGTCGGCGGGCCGACTCACAGCCAGCGGGAAATCA‑CTTgggc                                                                                             >  2:2501843/1‑149 (MQ=255)
                                                           cacaATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGC‑GCCGG‑CC‑C‑GC‑G‑CCGGTGCATAGGAGCGGAGCGGCGCGCCGGCACACAGGCCGCGGGAATTCAGGTTgggc                                                                                             >  2:2439498/1‑149 (MQ=255)
                                                               aTGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGG‑GGCGG‑CC‑C‑GCGC‑CCGTGGCA‑AGGAGCGGATCGGCGCGCCGGCACACAGCCACCCGGAAGTAA‑GTTGGggcgacg                                                                                        >  2:1482676/1‑150 (MQ=255)
                                                                 gAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGC‑GCCGG‑CC‑C‑GC‑G‑CCGTTTCATAGGAGCGGTTCGGCGCGCCGCCGCACAGCCAGCCGAAAATCA‑GTTGGgacgacgac                                                                                      >  2:816241/1‑150 (MQ=255)
                                                                  aGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGG‑GCCGG‑CC‑C‑GC‑G‑ACGTTGGATAGGCGCGGTTCGGCGCGCCCCCTCACAGCCAGCGGGCAATAA‑GGGGGGgcgacgacg                                                                                     >  2:2487178/1‑150 (MQ=255)
                                                                  aGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGG‑GCCGG‑CC‑C‑CC‑G‑CCGTTTCATAGGAGGCGGTCGGCGGGCCCCCAAACAGCCCCCCGGAATTCA‑GTTTGggcgacgaag                                                                                     >  1:10000047/1‑148 (MQ=255)
                                                                        tAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGG‑GCCGG‑GC‑C‑GC‑TCCCGTTG‑ATAGGAGCGGATCGGCGCGCCGCCCAACAGCCCCCGGGAATTAA‑GGTGGGGCGACGACCGCAccc                                                                               >  2:4320008/1‑149 (MQ=255)
                                                                          ggCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGG‑GCCGG‑CC‑C‑GC‑A‑CCGTTGCATAGGAGCGGATCGGCGCGCCGACTCACAGCCACCCGAAAATAA‑ATTGGGACGACGACGGCAccccc                                                                             >  2:3173306/1‑150 (MQ=255)
                                                                           gcaagcaaAATCAATGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGG‑GCCGG‑GC‑C‑GC‑T‑CCGGTGCGAAGGGGCGGGGCGGGGCGCCGACTCTCACGCCGCGGGAAATAT‑GGTGGGACGCGGACGGGAcccccg                                                                            >  2:3013359/1‑150 (MQ=255)
                                                                                     tCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGG‑GCCGG‑CC‑C‑CC‑G‑CCCTTGCAAAGGGGCGGGTCGGGGCGCCCCCCCCCAGCCCCCCGCAAATAA‑GGTTGGGCGACGGCGGCACCCCCCCGCCgggggg                                                                  >  2:941743/1‑147 (MQ=255)
                                                                                      cAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGC‑GCCGG‑CC‑C‑CC‑T‑CCGGTGCCTATGAGCGGATCGGCGCGCCGCCACACCTCCAGCGGCACTTAA‑AGTGGGTCGACCACGGCACCCCCGCCCAGGgcgcc                                                                 >  2:3402051/1‑149 (MQ=255)
                                                                                           gTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGG‑GCCGGCCC‑C‑CC‑C‑CCGTTCCAAAGG‑GCGGATGCGCGCGCCGACGACCATCCACCGGAAAATAA‑GTTGGGTAGAAGACCGCACCGCCCCGCAGGGGGCAGCggg                                                            >  2:3994045/1‑150 (MQ=255)
                                                                                           gTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGG‑GCCGG‑GC‑C‑GC‑G‑CCGGTGGTTAGGGGGGGCTGGGGGGGGCGACGCACAGCCCCCGGCACATCT‑GGGGGGTCGGCGACGGGCCCGGCGCGCGGGgcgggggggg                                                            >  2:9667211/1‑143 (MQ=255)
                                                                                            tttGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGG‑GCCCG‑CC‑C‑GC‑T‑GCGATGCATAGGAGCGGATAGGCGCGCCCCCACACAGCCAGCCGAACATAA‑GTTGGGACGACAACCGCACCGCCGCGCAGGGGGCGGCGGGc                                                           >  2:1792833/1‑150 (MQ=255)
                                                                                              tGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGC‑GCCGG‑CC‑C‑GC‑T‑CCGGTGCATAGTAGCGGGTCGGCGCGCCGACTCACAGCCAGCGGAAATTCA‑GGTTGGACGACGACGGCACCCCCCCGCGGGGCGCTGCGGGCTg                                                         >  1:5541249/1‑150 (MQ=255)
                                                                                               gCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGG‑GGCGG‑GC‑C‑GC‑C‑CCCGTGCATAGGAGCGGGGCAGCGCGGCCGCGCCCCGCCCGCGGGAATTGA‑TGTGGGGCGACGACGGCGCCCCCCCCCGGGGCGCGGCggggggg                                                        >  2:3153365/1‑146 (MQ=255)
                                                                                                ccGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGG‑GCCCG‑CC‑C‑CC‑G‑CCGGTGGATAGGGGGGGTTGGGGGCGCCGCCGCCCAGGCACCGGCAAATGA‑GGTTGGGCGGCGCCGGCACCCCCGCCCGGGGCGGGGcgggcgggg                                                       >  2:1149322/1‑149 (MQ=255)
                                                                                                   aTAGCCGAGGCTCCTTGCCCAGTGCATCGGCCGGTCGCGG‑GGCGG‑CC‑C‑GC‑G‑CCGTGGCCTTTGCGCGGGTCGGCGGCCCGCCGCACAGGCCGCGGGCATTAC‑GTGGGGACGACGCGGGGCCCGCCGCGCGGGGGGCTGAGGGGGGGCCgg                                                    >  2:6092366/1‑150 (MQ=255)
                                                                                                      gCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGCGGCCGG‑CCGC‑GC‑‑‑CCGTTGCA‑ATGGGCGGAGCGGGGCGCCCACTCACAGCCAGCGGGAATTAA‑CTTGGGCAGCCGTCCGCCCCCCCCCGCGGGGCGCGGCGGGCAGGCCGGAgg                                                 >  2:3580456/1‑150 (MQ=255)
                                                                                                         gAGGCTCCGTGCCCAGGGCTTCGGCGGGGCGCGC‑GCGGG‑CC‑C‑GC‑T‑CGGTTGCTTCGGCGCGGTTCGCCGCCCCGACTCACTGCCAGCGGAAATTCA‑GTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCAt                                              <  2:10034126/150‑1 (MQ=255)
                                                                                                           ggCTCCTTGCCCAGAGCATCGGCCGGTCGCGC‑GCCCG‑CC‑C‑CC‑G‑CCCTTGCATAGGAGGGGATCGGGGCGCCCACACACAGCCACCCGGAATTAA‑GTTGGGGAGCCGACGGGCCCGCCGCGCAGGGGGCGGCGGGGGGGGCGGAgggcaac                                            >  2:7831170/1‑145 (MQ=255)
                                                                                                            gccccTTCCCCAGACCATCGGCGGGCCGCGC‑GCCGG‑CC‑C‑CC‑C‑CCGTTGAATCGGCGCGGCTGGGCGCCCCGACTCACTGCCAGCGGAAATTCA‑GTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCa                                           <  2:7822289/147‑1 (MQ=255)
                                                                                                                 ttGCCCAGAGCATCGGCCGGTCGCGC‑GCCGG‑GC‑C‑CC‑A‑CCCGTTCATATGAGCGGGTCGGCGCGCCGACGCACTGCCCCCGGAAAATCA‑ATTGGGACGACCCCGGCACCCCCGCGCTGGGGGCGGCGGGGGGGGCCGCGGCCACCAGCGGt                                      >  2:5228493/1‑150 (MQ=255)
                                                                                                                 ttGCCCAGAGCATCGGCCGGTCGCGC‑GCCGG‑CC‑C‑GC‑G‑CCGGTGGATTGGAGCGGGTCGGCGCGCCGACGCACAGCCCGCGGGCATTCA‑GTTGGGACGGCGACGGGACCGCCCCGCGGGGCGGTGCGGGGTGGGCGGAGGCATCCAGCGGt                                      >  2:6299759/1‑150 (MQ=255)
                                                                                                                  ggCCCTGCGCCTCGGCCGGCCGCGC‑CCCGG‑CC‑C‑GC‑T‑CCGTGGCAGCGGAGCGCATCGGCGCCCCGACTCACTGCCAGCGGAAATTCA‑GTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTa                                     <  2:9872229/149‑1 (MQ=255)
                                                                                                                    cccAGAGCATCGGCCGGTCGCGG‑GCCCG‑CC‑C‑GC‑C‑CCCGTGGAGAGGTGCGGGTCGGCGCGCCCACACACAGCCACCGGAAAAAAA‑GTTTGGTCGACGCCGGCACCCCCCCGCGGGGGGCTGCGGGGTGGGCGGCGGCCGCCAGCGGGACg                                   >  2:5855861/1‑150 (MQ=255)
                                                                                                                     ccAGAGCCGCGCCGGGACGCGC‑GCCGG‑CC‑C‑GC‑G‑CCTGTCCATCGTTGGTGAGCGGCCCGCCGACTCACTGCCAGCGGAAATTCA‑GTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGc                                  <  1:1627746/150‑1 (MQ=255)
                                                                                                                     ccAGAGCATCGGCCGGTCGCGG‑GCCGG‑GC‑C‑CC‑G‑CCGGTGGAAATGAGCGGGTCGGCGCGCCCCCGCACCACCCCCCGGACATGA‑GGTGGGACGACGACGGCACCGCCCCGCGGGGCGCCGCGGGCGGGGCGGCGGCCACCCCCGGTACGc                                  >  2:5762449/1‑150 (MQ=255)
                                                                                                                      cAGAGCATCGGCCGGTCGCGG‑GCCCG‑CC‑C‑GC‑T‑CCCGTTCCTAGGGGCGGAGCGGCGCGGCGTCTACCAGGCAGCCGAAAATGA‑GTTGGGACCGCGGCGGCACCGCCCCGCGGGGGCCTGCGGGCTTGCCGGCGGGATACCGCCGTTCGcg                                 >  2:6815208/1‑149 (MQ=255)
                                                                                                                        gacccgcggcggGCCGCGC‑GCCGC‑CC‑G‑GC‑T‑CTGTTGCATGGGCGGGCCCGGGCGCCCCGACTCACTGCCAGCGGAAATTCA‑GTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGt                               <  1:3319836/144‑1 (MQ=255)
                                                                                                                         cgctgcGGCCGGGCGCGC‑CCCGG‑CC‑C‑GC‑T‑CGTTTGCTGAGGTGGGGCGGGGCGCCCCGACTCACTGCCAGCGGAAATTCA‑GTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTc                              <  1:10708160/145‑1 (MQ=255)
                                                                                                                          gatgcggcggGTCGCGC‑GCGGG‑CC‑C‑GC‑G‑CCGTTGCATCGGTGCGGATGGGCGCGCCGACTCACTGCCAGCGGAAATTCA‑GTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCg                             <  2:7069565/146‑1 (MQ=255)
                                                                                                                          cgcccggccggcCCCCG‑GCCGC‑CC‑C‑GC‑C‑CGGTTGCATGGGCGCGCACCGGCGCGCCGACTCACTGCCAGCGGAAATTCA‑GTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCg                             <  2:8358713/146‑1 (MQ=255)
                                                                                                                                      cgcgc‑gCCGG‑CC‑C‑GC‑T‑CCGTTGCATAGGAGCGGATCGGCGCGCCGACTCCCTGCCAGCGGGAATTCA‑GTTGGGGCGTCGACGGGACCGCCGCGCGGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGGtttac                 >  1:2741504/1‑148 (MQ=255)
                                                                                                                                       gcgc‑gcCGG‑CC‑C‑GC‑T‑CCTTTTCTTTGGCGCGGCCGGGCGCCCCGACTCACTGCCAGCGGAAATTCA‑GTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCt                <  1:5351092/150‑1 (MQ=255)
                                                                                                                                             ccGG‑CC‑C‑GC‑T‑CCGTTGCATAGGAGCGGATCGGCGCGCCGACTCACTGCCAGCGGAAATTCA‑GTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCAAc           >  1:10761827/1‑150 (MQ=255)
                                                                                                                                             ccGG‑CC‑C‑GC‑T‑CCGTTGCATAGGAGCGGATCGGCGCGCCGACTCACTGCCAGCGGAAATTCA‑GTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCAAc           >  2:3923374/1‑150 (MQ=255)
                                                                                                                                              cGG‑CC‑C‑GC‑T‑CCGTTGCATAGGAGCGGATCGGCGCGCCGACTCACTGCCAGCGGAAATTCA‑GTGGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCAACt          >  2:901718/1‑150 (MQ=255)
                                                                                                                                               cg‑CC‑C‑CC‑T‑CCGTTCCAGAGTGGGGGACCGGCCCCCCGACTCACTGCCAGCGGAAATTCA‑GTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCAACTg         <  1:77119/149‑1 (MQ=255)
                                                                                                                                                g‑CC‑C‑GC‑T‑CCGTTGCATAGGAGCGGATCGGCGCGCCGACTCACTGCCAGCGGAAATTCA‑GTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCAACTgc        >  1:259090/1‑150 (MQ=255)
                                                                                                                                                g‑CC‑C‑GC‑T‑CCGTTGCATAGGAGCGGATCGGCGCGCCGACTCACTGCCAGCGGAAATTCA‑GTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCAACTgc        >  2:10360320/1‑150 (MQ=255)
                                                                                                                                                  cc‑c‑GC‑T‑CCGTTGCATAGGAGCGGATCGGCGCGCCGACTCACTGCCAGCGGAAATTCA‑GTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCCGCGGTACGCTGTCGGCCGGGATTTCCTGCAACTgcg       >  1:2065452/1‑150 (MQ=255)
                                                                                                                                                  cc‑c‑GC‑T‑CCGTTGCATAGGAGCGGATCGGCGCGCCGACTCACTGCCAGCGGAAATTCA‑GTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCAACTgcg       >  2:1118319/1‑150 (MQ=255)
                                                                                                                                                     c‑GC‑T‑CCGTTGCATAGGAGCGGATCGGCGCGCCGACTCACTGCCAGCGGAAATTCA‑GTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCCGCGGTACGCTGTCGGCCGGGATTTCCTGCAACTGCGcc     >  2:7846915/1‑150 (MQ=255)
                                                                                                                                                     c‑GC‑T‑CCGTTGCATAGGAGCGGATCGGCGCGCCGACTCACTGCCAGCGGAAATTCA‑GTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCCACTGCGcc     >  1:4565690/1‑150 (MQ=255)
                                                                                                                                                     c‑GC‑T‑CCGTTGCATAGGAGCGGATCGGCGCGCCGACTCACTGCCAGCGGAAATTCA‑GTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCAACTGCGcc     >  1:4565897/1‑150 (MQ=255)
                                                                                                                                                       gC‑T‑CCGTTGCATAGGAGCGGATCGGCGCGCCGACACACTGCCAGCGGAAATTCA‑GTTGGGAAGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTACTTCAACTGCGCCa    >  1:2053591/1‑150 (MQ=255)
                                                                                                                                                          t‑CCGTTGCATAGGAGCGGATCGGCGCGCCGACTCACTGCCAGCGGAAATTCA‑GTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCAACTGCGCCAgg  >  2:7520409/1‑150 (MQ=255)
                                                                                                                                                          |                                                                                                                                                       
CGAAAACGCCGCAGGAGTCACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGC‑GCCGG‑CC‑C‑GC‑T‑CCGTTGCATAGGAGCGGATCGGCGCGCCGACTCACTGCCAGCGGAAATTCA‑GTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCAACTGCGCCAGG  >  NC_008463/4616301‑4616599

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑