Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084634,616,4540TG34.9% 244.8 / 32.7 154intergenic (‑174/+28)hda/PA14_RS21180DnaA regulatory inactivator Hda/DUF2066 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base T (55/44);  new base G (50/3);  total (107/47)
Fisher's exact test for biased strand distribution p-value = 2.76e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 158 total aligned reads displayed.

AACGCCGCAGGAGTCACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGC‑GCGCCGGCCC‑GC‑TCC‑GTTGCATAGGAGCGGATCGGCGCGCCGACTCA‑CTGCCAGCGGAAATTCA‑GTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCAACTGCGCCAGGCCCA  >  NC_008463/4616305‑4616603
                                                                                                                                                         |                                                                                                                                                       
aaCGCCGCAGGAGTCACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGC‑GCGCCGGCCC‑GC‑TCC‑Gt                                                                                                                                                         <  1:4280314/150‑1 (MQ=255)
   gccgcAGGAGTCACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGC‑GGGCCGGCCC‑CG‑TCC‑GCTGc                                                                                                                                                      >  2:6347056/1‑150 (MQ=255)
   gccgcAGGAGTCACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGC‑GCGCCGGCCC‑GC‑TCC‑GTTGc                                                                                                                                                      <  1:5749650/150‑1 (MQ=255)
   gccgcAGGAGTCACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGC‑GCGCCGGCCC‑GC‑TCC‑CTTGc                                                                                                                                                      >  2:6265551/1‑150 (MQ=255)
    ccgcAGGAGTCACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCACAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGC‑GCGCCGGCCC‑GC‑TCC‑GTTGCa                                                                                                                                                     <  1:5217032/150‑1 (MQ=255)
    ccgcAGGAGTCACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGC‑GCGCCCGCCC‑CC‑CCC‑GGTGCa                                                                                                                                                     >  2:7412205/1‑150 (MQ=255)
     cgcAGGAGTCACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGC‑GCGCCGGCCC‑GG‑TCC‑GGAGCAt                                                                                                                                                    >  2:6901198/1‑150 (MQ=255)
       cAGGAGTCACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGC‑GCGCCGGCCC‑GC‑TCC‑GTTGCATAg                                                                                                                                                  <  2:5008255/150‑1 (MQ=255)
        aGGAGTCACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGC‑GCGCCGGCCC‑CC‑GCC‑GTTggagatg                                                                                                                                                 >  2:568355/1‑144 (MQ=255)
         ggAGTCACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGC‑GCGCCGGCCC‑GC‑TCC‑GTTGCATAGGa                                                                                                                                                <  1:6861750/150‑1 (MQ=255)
          gAGTCACCGTAAAGGCTGCAAAGTTTATCCGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGC‑GCGCCGGCCC‑GC‑TCC‑GTTGCATAGGAg                                                                                                                                               <  1:8558654/150‑1 (MQ=255)
           aGTCACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGC‑GCGCCGGCCC‑GC‑TCC‑GTTGCATAGGAGc                                                                                                                                              <  1:4542427/150‑1 (MQ=255)
            gTCACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGC‑GGGCCGGCCC‑CC‑GCC‑GGTGGCTAggcggg                                                                                                                                             >  2:140713/1‑146 (MQ=255)
            gTCACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGC‑GCGCCGGCCC‑GC‑TCC‑GTTGCATAGGAGCg                                                                                                                                             <  2:10871856/150‑1 (MQ=255)
            gTCACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGC‑GCGCCCGCCC‑CC‑TCC‑GGTGCAAAGGAGCg                                                                                                                                             >  2:8862530/1‑150 (MQ=255)
             tCACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGC‑GCGCCGGCCC‑CC‑GCC‑GGTGCATATGAGCgg                                                                                                                                            >  2:4279087/1‑150 (MQ=255)
              cACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGC‑GCGCCGGCCC‑GC‑TCC‑GTTGCATAGGAGCGGa                                                                                                                                           <  1:3840986/150‑1 (MQ=255)
              cACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGC‑GCGCCGGCCC‑GC‑TCC‑GTTGCATAGGAGCGGa                                                                                                                                           <  1:5395154/150‑1 (MQ=255)
               aCCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGC‑GGGGCGGCCC‑GC‑TCC‑CGTGCAGAggggggggt                                                                                                                                          >  1:6414493/1‑143 (MQ=255)
               aCCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGC‑GCGCCGGCCC‑GC‑TCC‑GTTGCATAGGAGCGGAt                                                                                                                                          <  1:9351195/150‑1 (MQ=255)
               aCCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGC‑GCGCCGGCCC‑GC‑TCC‑GTTGCATAGGAGCGGAt                                                                                                                                          <  1:6146533/150‑1 (MQ=255)
               aCCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGC‑GCGCCGGCCC‑CC‑GCC‑CTTGCATAGGAGCggct                                                                                                                                          >  2:9162479/1‑148 (MQ=255)
               aCCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGC‑GCGCCCGGCC‑GC‑GCC‑CGTGCAAAGGAGGGGag                                                                                                                                          >  2:4643376/1‑149 (MQ=255)
                ccGTCAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAACATCCAAGTTTTGCCGATCGCCGAGGCTCCTGGCCCAGAGCATCGGCCGGTCGC‑GCGCCGGCCC‑GC‑TCC‑GTTGCATAGGAGCGGATc                                                                                                                                         <  2:1394270/150‑1 (MQ=255)
                ccGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGC‑GCGCCGGCCC‑GC‑TCC‑GTTGCATAGGAGCGGATc                                                                                                                                         <  2:8331854/150‑1 (MQ=255)
                  gTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGC‑GCGCCGGCCC‑GG‑CCC‑GGTTCTTAGGAGCGGagccg                                                                                                                                       >  2:9570785/1‑146 (MQ=255)
                  gTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACNAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGC‑GCGCCGGCCC‑GC‑GAC‑CGTGCCTAGGAggggcggcg                                                                                                                                       >  1:5788265/1‑145 (MQ=255)
                         cTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGCGCAACTTGGCAAGCAAAATCAAAGGTTGGCCTATAGCCGAGGATCCTTGCCCAGAGCATCGGCCGGTCGC‑GCGCCGGCCC‑GC‑TCC‑GTTGCTTAGGTGCGGATCGGCGCGCCg                                                                                                                                <  1:10994786/150‑1 (MQ=255)
                                 tttATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGC‑GCGCCGGCCC‑GC‑GCC‑GGGTGATAGGGGCGGGTCGGCGCGCCGCCaca‑cag                                                                                                                       >  1:6133116/1‑148 (MQ=255)
                                  ttATACGCCTCGGCAAGGCCGCAAAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCAGATATCCGAGTCTCCTTTCCCAGAGCATCGGCCGATCGA‑GGGACGCCCC‑AC‑ACA‑GGTTGATAGGAGCGGAACGGAGCGCCGACACC‑CTGc                                                                                                                      >  2:9747393/1‑150 (MQ=255)
                                   tataCGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGC‑GGGGCGGGCC‑GC‑GAC‑GGTGCTTAGGTGCGGGTCGGCGCGCCCACGCA‑caggc                                                                                                                     >  1:6101451/1‑146 (MQ=255)
                                   tataCGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGC‑GGGCCGGCCC‑GC‑GCC‑GTTTGCTATGAGCGGGTAGGCGCGCCGACACA‑CAGcc                                                                                                                     >  2:4010435/1‑150 (MQ=255)
                                        gCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGC‑GGGCCGGCCCGGG‑CCC‑GTTGC‑TAGGGGGGGGTGGGCCCGCCCACGCA‑CTGCCAGccg                                                                                                                >  1:11017950/1‑148 (MQ=255)
                                             ggCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGC‑GCGCCCGCCC‑GC‑GCC‑GGTGGATAGGAGCGGGTCGGCGCGCCGCCTCA‑CTGCCACCGGAAAtt                                                                                                           >  2:8622699/1‑150 (MQ=255)
                                               cAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGC‑GGGCCCGCCC‑CC‑GCC‑GGTTGAAAGGGTCGGGTGGGGGCGCCGACACC‑ATGCCCGCGGaaaatta                                                                                                         >  1:8126774/1‑146 (MQ=255)
                                                 aGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGC‑GCGCCCGCCC‑CC‑GAC‑GTTGCCTAGGAGCGGGTCGGCGCGCCGACGCC‑CAGCCAGCGGTCAATCA‑Gt                                                                                                      >  2:6284651/1‑150 (MQ=255)
                                                   gCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGC‑GCGCCGGCCC‑GC‑TCC‑CTTTCATAGGAGCGGATAGGGGCGCCGACTCA‑CTGCCAGCGGAAAATAA‑GTTg                                                                                                    >  2:9124179/1‑150 (MQ=255)
                                                      gCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGC‑GGGCCGGCCC‑CC‑GCC‑GTTGCATAGGAGCGGGTCGGCGGGCCGACTCA‑CAGCCAGCGGGAAATCA‑CTTgggc                                                                                                 >  2:2501843/1‑149 (MQ=255)
                                                           aTGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGC‑GGGGCGGCCC‑GCGCCC‑GTGGCA‑AGGAGCGGATCGGCGCGCCGGCACA‑CAGCCACCCGGAAGTAA‑GTTGGggcgacg                                                                                            >  2:1482676/1‑150 (MQ=255)
                                                           aTGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGC‑GCGCCCGCCC‑GC‑GCCGGGTGC‑TAGGAGGGGGACGGCGCGCCGGCACA‑AAGCCCGCGGAGATAAA‑GTGGGggcgacg                                                                                            >  2:854845/1‑150 (MQ=255)
                                                             gAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGC‑GCGCCGGCCC‑GC‑GCC‑GTTTCATAGGAGCGGTTCGGCGCGCCGCCGCA‑CAGCCAGCCGAAAATCA‑GTTGGgacgacgac                                                                                          >  2:816241/1‑150 (MQ=255)
                                                              aGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGC‑GGGCCGGCCC‑GC‑GAC‑GTTGGATAGGCGCGGTTCGGCGCGCCCCCTCA‑CAGCCAGCGGGCAATAA‑GGGGGGgcgacgacg                                                                                         >  2:2487178/1‑150 (MQ=255)
                                                              aGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGC‑GGGCCGGCCC‑CC‑GCC‑GTTTCATAGGAGGCGGTCGGCGGGCCCCCAAA‑CAGCCCCCCGGAATTCA‑GTTTGggcgacgaag                                                                                         >  1:10000047/1‑148 (MQ=255)
                                                                  aCTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGC‑GCGCCGGCCC‑CC‑GCC‑GTTGCATAGGAGCGGATACGCGCGCCGACTCA‑CTGCCCGCGGGAATTCA‑GTTGGGGCGCCGACGGCAc                                                                                     >  2:7941022/1‑150 (MQ=255)
                                                                      ggCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGC‑GGGCCGGCCC‑GC‑ACC‑GTTGCATAGGAGCGGATCGGCGCGCCGACTCA‑CAGCCACCCGAAAATAA‑ATTGGGACGACGACGGCAccccc                                                                                 >  2:3173306/1‑150 (MQ=255)
                                                                       gcaagcaaAATCAATGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGC‑GGGCCGGGCC‑GC‑TCC‑GGTGCGAAGGGGCGGGGCGGGGCGCCGACTCT‑CACGCCGCGGGAAATAT‑GGTGGGACGCGGACGGGAcccccg                                                                                >  2:3013359/1‑150 (MQ=255)
                                                                           gcaaAATCAATGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGC‑GGGCCCGCCC‑CC‑GCC‑GTTTCCTAAGGGCGGATCGGCGCGCCCACGCA‑AAGCCCCCGGGAAATAA‑GTTGGGGCCACGGAGGCCCCGCCgcgcc                                                                            >  2:2443207/1‑149 (MQ=255)
                                                                                 tCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGC‑GGGGCGGCCC‑CCGCCC‑GTTG‑ATTTGAGCGGGTAGGCGGGCCCGCCCA‑CAGGCAGCCGAAATTAA‑TTTGGGACGTCGACGGCACCCCCGCGCGGGgggc                                                                      >  2:2750669/1‑150 (MQ=255)
                                                                                 tCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGC‑GGGCCGGCCC‑CC‑GCC‑CTTGCAAAGGGGCGGGTCGGGGCGCCCCCCCC‑CAGCCCCCCGCAAATAA‑GGTTGGGCGACGGCGGCACCCCCCCGCCgggggg                                                                      >  2:941743/1‑147 (MQ=255)
                                                                                  cAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGC‑GCGCCGGCCC‑CC‑TCC‑GGTGCCTATGAGCGGATCGGCGCGCCGCCACA‑CCTCCAGCGGCACTTAA‑AGTGGGTCGACCACGGCACCCCCGCCCAGGgcgcc                                                                     >  2:3402051/1‑149 (MQ=255)
                                                                                    aaGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGC‑GCGCCCGCCC‑GC‑TCC‑CGTGGCTTGGAGCCGAAAGGCGCGCCGACAAA‑CGGCCAGCCGCAATAAC‑GTTGGGCCGACCAAGGCACCCCCGCGCTGGgcgcggg                                                                   >  2:5203831/1‑147 (MQ=255)
                                                                                     aGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGC‑GGGCCGGCCC‑CC‑ACC‑CGTGGGTAGGAGCGGGTGCGCGCCCCCACACA‑CAACCCCCGGCCAATGA‑GTGTGGGGGCCGACGGCACCGCCGCGCGGGGGgcggcg                                                                  >  2:1135660/1‑150 (MQ=255)
                                                                                     aGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGC‑GCGCCGGCCC‑CC‑GCC‑CGTGGATTGGAGCGGGTCGGCGCGCCGCCCCG‑CGGCCCGCGGGAAATAA‑GGTGGGACGCCGACGGCACCCCCGCGCGGGGCgcggcg                                                                  >  2:351514/1‑150 (MQ=255)
                                                                                       gTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGC‑GGGCCGGGCC‑GC‑GCC‑GGTGGTTAGGGGGGGCTGGGGGGGGCGACGCA‑CAGCCCCCGGCACATCT‑GGGGGGTCGGCGACGGGCCCGGCGCGCGGGgcgggggggg                                                                >  2:9667211/1‑143 (MQ=255)
                                                                                        tttGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGC‑GGGCCCGCCC‑GC‑TGC‑GATGCATAGGAGCGGATAGGCGCGCCCCCACA‑CAGCCAGCCGAACATAA‑GTTGGGACGACAACCGCACCGCCGCGCAGGGGGCGGCGGGc                                                               >  2:1792833/1‑150 (MQ=255)
                                                                                           gCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGC‑GGGGCGGGCC‑GC‑CCC‑CGTGCATAGGAGCGGGGCAGCGCGGCCGCGCC‑CCGCCCGCGGGAATTGA‑TGTGGGGCGACGACGGCGCCCCCCCCCGGGGCGCGGCggggggg                                                            >  2:3153365/1‑146 (MQ=255)
                                                                                            ccGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGC‑GGGCCCGCCC‑CC‑GCC‑GGTGGATAGGGGGGGTTGGGGGCGCCGCCGCC‑CAGGCACCGGCAAATGA‑GGTTGGGCGGCGCCGGCACCCCCGCCCGGGGCGGGGcgggcgggg                                                           >  2:1149322/1‑149 (MQ=255)
                                                                                             cGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGCGGGGCCGCCCC‑CG‑ACC‑GTTGC‑TAGGTGCGGCTCGGCGCGCCGACGCACCTGCC‑CCGGGCAATAA‑TGTGGGGCGGCGACGGGCACGCCGCGCGGGGGGCGGCggggggggc                                                          >  2:5704220/1‑144 (MQ=255)
                                                                                                     gAGGCTCCGTGCCCAGGGCTTCGGCGGGGCGC‑GCGCGGGCCC‑GC‑TCG‑GTTGCTTCGGCGCGGTTCGCCGCCCCGACTCA‑CTGCCAGCGGAAATTCA‑GTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCAt                                                  <  2:10034126/150‑1 (MQ=255)
                                                                                                       ggCTCCTTGCCCAGAGCATCGGCCGGTCGC‑GGGCCCGCCC‑CG‑GGC‑GTTGACGGGGTGCGGTACGGCGGCCCGCCACA‑CGGCCCGCCGCAATTCA‑GTTGGGGCGGCGGCGGGACCCGCGCGCGGGGGGCTGCGGGCTGGCCGGAGGCAAcc                                                >  2:7830659/1‑150 (MQ=255)
                                                                                                       ggCCCCTGGCCCAGCGCTCGGGCCGGGCGC‑CCCCCGCCCC‑GC‑TCG‑GTTGCAGCGCCGCGTCGCGGCGCGCCGACTCA‑CTGCCAGCGGAAATTCA‑GTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATcc                                                <  2:3470574/150‑1 (MQ=255)
                                                                                                        gccccTTCCCCAGACCATCGGCGGGCCGC‑GCGCCGGCCC‑CC‑CCC‑GTTGAATCGGCGCGGCTGGGCGCCCCGACTCA‑CTGCCAGCGGAAATTCA‑GTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCa                                               <  2:7822289/147‑1 (MQ=255)
                                                                                                          tCCTTGCCCAGAGCATCGGCCGGTCGC‑GCGCCCGCCC‑GC‑GCC‑GGTGCCTAGGCGCGGCTCGGCGCGCCGACACC‑CGGGCCCCGGCTATTAA‑GTTGGGTCGCCGAAGGGACCCCCCCGCAGGGCGCTGCGGGGGGGCCGGAGGCCTCCAgg                                             >  2:7061710/1‑149 (MQ=255)
                                                                                                          cccTTCCCCACAGCTGCGGCGGGGCCC‑GGCCCGGCCC‑GG‑GCC‑TGTGCATCGGAGCGCCGGGGCCCCCCGACTCA‑CTGCCAGCGGAAATTCA‑GTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGc                                             <  1:7826126/149‑1 (MQ=255)
                                                                                                           ccTTGCCCAGAGCATCGGCCGGTCGC‑GGGCCCGGCC‑GCGGCC‑GTGGC‑TAGGGGCGGGTCGGGGCGCCCGCGAC‑CAGGCCGCCGGACTTCA‑GGTGGGGCGGCCGCGGCACCGCCGCGCGGGGGGGCGCGGGGGGGGCGGCGGCCTcccccg                                            >  2:9512001/1‑146 (MQ=255)
                                                                                                           ccTTGCCCAGAGCATCGGCCGGTCGC‑GCGCCGGCCC‑GC‑CCC‑GGTGCATTGGAGCGGCTCCGCGCGCCCACACC‑AAGCCCCCCGGAAATCA‑CTTGGGTCCACCACCGCACCCCCCCCCAGGGCGCGGCGGGCTGGCCGGAGGGATACACCg                                            >  2:5925929/1‑150 (MQ=255)
                                                                                                             ttGCCCAGAGCATCGGCCGGTCGC‑GGGCCCGCCC‑CC‑GCC‑GGTGGAAAGGAGCGGGTCGGCGCGCCCGCACA‑CATCCAGCGGAAATTAATGTTGGG‑CGAAGAAGGCAACCCCCCCCAGGGCGGTGCGGGGTTGGCGGGGGCAAACAGCGGt                                          >  2:1577635/1‑150 (MQ=255)
                                                                                                             ttGCCCAGAGCATCGGCCGGTCGC‑GCGCCGGGCC‑CC‑ACC‑CGTTCATATGAGCGGGTCGGCGCGCCGACGCA‑CTGCCCCCGGAAAATCA‑ATTGGGACGACCCCGGCACCCCCGCGCTGGGGGCGGCGGGGGGGGCCGCGGCCACCAGCGGt                                          >  2:5228493/1‑150 (MQ=255)
                                                                                                              ggCCCTGCGCCTCGGCCGGCCGC‑GCCCCGGCCC‑GC‑TCC‑GTGGCAGCGGAGCGCATCGGCGCCCCGACTCA‑CTGCCAGCGGAAATTCA‑GTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTa                                         <  2:9872229/149‑1 (MQ=255)
                                                                                                               gCCCAGAGCATCGGCCGGTCGC‑GGGCCCGCCC‑CC‑ACC‑GGTTGATAGGTGCGGGGGGGCGCGCCGCCACA‑CAGCCAGCGGTAAATCA‑TGTGGGACGACGAACGCCCCCCCCCCCGTGGCGCGGCGGGCGGGGCGGAGGCCTCCCGCgggaa                                        >  2:4503270/1‑147 (MQ=255)
                                                                                                                cccAGAGCATCGGCCGGTCGC‑GGGCCCGCCC‑GC‑CCC‑CGTGGAGAGGTGCGGGTCGGCGCGCCCACACA‑CAGCCACCGGAAAAAAA‑GTTTGGTCGACGCCGGCACCCCCCCGCGGGGGGCTGCGGGGTGGGCGGCGGCCGCCAGCGGGACg                                       >  2:5855861/1‑150 (MQ=255)
                                                                                                                 ccAGAGCATCGGCCGGTCGC‑GGGCCGGGCC‑CC‑GCC‑GGTGGAAATGAGCGGGTCGGCGCGCCCCCGCA‑CCACCCCCCGGACATGA‑GGTGGGACGACGACGGCACCGCCCCGCGGGGCGCCGCGGGCGGGGCGGCGGCCACCCCCGGTACGc                                      >  2:5762449/1‑150 (MQ=255)
                                                                                                                  cAGAGCATCGGCCGGTCGC‑GGGCCCGCCC‑GC‑TCC‑CGTTCCTAGGGGCGGAGCGGCGCGGCGTCTAC‑CAGGCAGCCGAAAATGA‑GTTGGGACCGCGGCGGCACCGCCCCGCGGGGGCCTGCGGGCTTGCCGGCGGGATACCGCCGTTCGcg                                     >  2:6815208/1‑149 (MQ=255)
                                                                                                                    ctccagcGGCCGGAGGG‑GCCCCGCCCC‑GC‑TCG‑GGTCATTCGGAGCGGAGGGGCGCGCCGACTCA‑CTGCCAGCGGAAATTCA‑GTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGt                                   <  2:7718501/144‑1 (MQ=255)
                                                                                                                     cgctgcGGCCGGGCGC‑GCCCCGGCCC‑GC‑TCG‑TTTGCTGAGGTGGGGCGGGGCGCCCCGACTCA‑CTGCCAGCGGAAATTCA‑GTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTc                                  <  1:10708160/145‑1 (MQ=255)
                                                                                                                      gatgcggcggGTCGC‑GCGCGGGCCC‑GC‑GCC‑GTTGCATCGGTGCGGATGGGCGCGCCGACTCA‑CTGCCAGCGGAAATTCA‑GTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCg                                 <  2:7069565/146‑1 (MQ=255)
                                                                                                                        ccgggCGGGGCGC‑GCGCCGCCCC‑GC‑TCC‑TTGCCATGGGTGGGGCCCGGCGCCCCGACTCA‑CTGCCAGCGGAAATTCA‑GTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGc                               <  1:3241413/147‑1 (MQ=255)
                                                                                                                              gggTGGC‑GCGCGGGCCC‑GC‑GCG‑GTCGCTTGGGCCGGGAGCGGCGCCCCGACTCA‑CTGCCAGCGGAAATTCA‑GTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCGGTCGGCCGGGAt                         <  2:3967448/149‑1 (MQ=255)
                                                                                                                                  cgc‑gcgCCGGCCC‑GC‑TCC‑GTTGCATAGGAGCGGATCGGCGCGCCGACTCC‑CTGCCAGCGGGAATTCA‑GTTGGGGCGTCGACGGGACCGCCGCGCGGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGGtttac                     >  1:2741504/1‑148 (MQ=255)
                                                                                                                                   gc‑gcgcCGGCCC‑GC‑TCC‑TTTTCTTTGGCGCGGCCGGGCGCCCCGACTCA‑CTGCCAGCGGAAATTCA‑GTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCt                    <  1:5351092/150‑1 (MQ=255)
                                                                                                                                         ccGGCCC‑GC‑TCC‑GTTGCATAGGAGCGGATCGGCGCGCCGACTCA‑CTGCCAGCGGACATTCA‑GTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCAAc               >  1:9353745/1‑150 (MQ=255)
                                                                                                                                         ccGGCCC‑GC‑TCC‑GTTGCATAGGAGCGGATCGGCGCGCCGACTCA‑CTGCCAGCGGAAATTCA‑GTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCAAc               >  1:10761827/1‑150 (MQ=255)
                                                                                                                                         ccGGCCC‑GC‑TCC‑GTTGCATAGGAGCGGATCGGCGCGCCGACTCA‑CTGCCAGCGGAAATTCA‑GTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCAAc               >  2:3923374/1‑150 (MQ=255)
                                                                                                                                          cGGCCC‑GC‑TCC‑GTTGCATAGGAGCGGATCGGCGCGCCGACTCA‑CTGCCAGCGGAAATTCA‑GTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCAACt              >  1:9705022/1‑150 (MQ=255)
                                                                                                                                          cGGCCC‑GC‑TCC‑GTTGCATAGGAGCGGATCGGCGCGCCGACTCA‑CTGCCAGCGGAAATTCA‑GTGGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCAACt              >  2:901718/1‑150 (MQ=255)
                                                                                                                                           ggCCC‑GC‑TCC‑GTTGCATAGGAGCGGATCGGCGCGCCGACTCA‑CTGCCAGCGGAAATTCA‑GTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCAACTg             >  1:1937961/1‑150 (MQ=255)
                                                                                                                                           cgCCC‑GC‑TCC‑GTTCCACCGGCGCGCCGCGCCCCCCCGACTCA‑CTGCCAGCGGAAATTCA‑GTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCAACTg             <  1:4638191/149‑1 (MQ=255)
                                                                                                                                            gCCC‑GC‑TCC‑GTTGCCTAGGAGCGGATCGGCGCGCCGACTCA‑CTGCCAGCGGAACTTCA‑GGTGGGACGACGACGGCACCGCCGCGCGGGGCGCTGCGGGCTGGGCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCCACTgc            >  2:10371777/1‑150 (MQ=255)
                                                                                                                                            gCCC‑GC‑TCC‑GTTGCATAGGAGCGGATCGGCGCGCCGACTCA‑CTGCCAGCGGAAATTCA‑GTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCAACTgc            >  1:259090/1‑150 (MQ=255)
                                                                                                                                            gCCC‑GC‑TCC‑GTTGCATAGGAGCGGATCGGCGCGCCGACTCA‑CTGCCAGCGGAAATTCA‑GTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCAACTgc            >  1:130997/1‑150 (MQ=255)
                                                                                                                                            gCCC‑GC‑TCC‑GTTGCATAGGAGCGGATCGGCGCGCCGACTCA‑CTGCCAGCGGAAATTCA‑GTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCAACTgc            >  1:10769472/1‑150 (MQ=255)
                                                                                                                                             ccc‑GC‑TCC‑GTTGCATAGGAGCGGATCGGCGCGCCGACTCA‑CTGCCAGCGGAAATTCA‑GTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCAACTgcg           >  2:1114899/1‑150 (MQ=255)
                                                                                                                                               c‑gc‑gCC‑GTTGCATAGGAGCGGATCGGCGCGCCGACTCA‑CTGCCAGCGGACATTCC‑GGTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTGCGCTGTCGGCCGGGATGTCCTGCCGCTgcgcg         >  2:7981654/1‑149 (MQ=255)
                                                                                                                                               c‑GC‑TCC‑GTTGCATAGGAGCGGATCGGCGCGCCGACTCA‑CTGCCAGCGGAAATTCA‑GTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCCGCGGTACGCTGTCGGCCGGGATTTCCTGCAACTGCGcc         >  2:7846915/1‑150 (MQ=255)
                                                                                                                                               c‑GC‑TCC‑GTTGCATAGGAGCGGATCGGCGCGCCGACTCA‑CTGCCAGCGGAAATTCA‑GTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCAACTGCGcc         >  1:4565897/1‑150 (MQ=255)
                                                                                                                                                 gC‑TCC‑GTTGCATAGGAGCGGATCGGCGCGCCGACACA‑CTGCCAGCGGAAATTCA‑GTTGGGAAGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTACTTCAACTGCGCCa        >  1:2053591/1‑150 (MQ=255)
                                                                                                                                                    tCC‑GTTGCATAGGAGCGGATCGGCGCGCCGACTCA‑CTGCCAGCGGAAATTCA‑GTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCAACTGCGCCAgg      >  1:8409929/1‑150 (MQ=255)
                                                                                                                                                    tCC‑GTTGCATAGGAGCGGATCGGCGCGCCGACTCA‑CTGCCAGCGGAAATTCA‑GTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCAACTGCGCCAgg      >  1:9027721/1‑150 (MQ=255)
                                                                                                                                                    tCC‑GTTGCATAGGAGCGGATCGGCGCGCCGACTCA‑CTGCCAGCGGAAATTCA‑GTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCAACTGCGCCAgg      >  2:7520409/1‑150 (MQ=255)
                                                                                                                                                        gTTGCATAGGAGCGGATCGGCGCGCCGACTCA‑CTGCCAGCGGAAATTCA‑GTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCAACTGCGCCAGGccc   >  1:2243263/1‑150 (MQ=255)
                                                                                                                                                         |                                                                                                                                                       
AACGCCGCAGGAGTCACCGTAAAGGCTGCAAAGTTTATACGCCTCGGCAAGGCCGCACAATGAGCAACTAGGCAAGCAAAATCAAAGGTTTGCCGATAGCCGAGGCTCCTTGCCCAGAGCATCGGCCGGTCGC‑GCGCCGGCCC‑GC‑TCC‑GTTGCATAGGAGCGGATCGGCGCGCCGACTCA‑CTGCCAGCGGAAATTCA‑GTTGGGACGACGACGGCACCGCCGCGCTGGGCGCTGCGGGCTGGCCGGAGGCATCCAGCGGTACGCTGTCGGCCGGGATTTCCTGCAACTGCGCCAGGCCCA  >  NC_008463/4616305‑4616603

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑