Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084635,248,9500TG23.4% 971.2 / 16.5 361L666R (CTG→CGG) tpsA4two‑partner secretion system exoprotein TpsA4
Reads supporting (aligned to +/- strand):  ref base T (48/201);  new base G (74/2);  total (158/203)
Fisher's exact test for biased strand distribution p-value = 1.63e-37
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 367 total aligned reads displayed.

GAGCAGCGAGAATGTCGGTTCCGGCCTGGCGAATCCAAGCAGCGCGGGAAAAGGTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCCTGGGACAGCGGCAACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGGCGCGCTGACGGCCCAGGGGGAAAACGCCAGCCTTACTCTCAATGCCGGGCAGCGCCCTCTCCGTATCGACGACAGCCTCTCTCTCA  >  NC_008463/5248801‑5249099
                                                                                                                                                     |                                                                                                                                                     
   cagcGAGAATGTCGGTTCCGGCCTGGCGAATCCAAGCAGCGCGGGAAAAGGTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTc                                                                                                                                                    <  1:1072218/150‑1 (MQ=255)
    agcGAGAATGTCGGTTCCGGCCTGGCGAATCCAATCAGCGCGGGAAAAGGTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAATTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCCCTGTcc                                                                                                                                                   >  2:3483418/1‑150 (MQ=255)
     gcGAGAATGTCGGTTCCGGCCTGGCGAATCCAAGCAGCGCGGGAAAAGGTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGGCGCGGTccg                                                                                                                                                  >  1:9249364/1‑149 (MQ=255)
     gcGAGAATGTCGGTTCCGGCCTGGCGAATCCAAGCAGCGCGGGAAAAGGTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCCt                                                                                                                                                  <  2:8843937/150‑1 (MQ=255)
      cGAGAATGTCGGTTCCGGCCTGGCGAATCCAAGCAGCGCGGGAAAAGGTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCACCGTCGAAGTGGTCGCCACACGGGGCAACGCGCATGTCGGCGCGCCGCTGtgcgg                                                                                                                                                 >  2:5333605/1‑146 (MQ=255)
      cGAGAATGTCGGTTCCGGCCTGGCGAATCCAAGCAGCGCGGGAAAAGGTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCCTg                                                                                                                                                 <  2:8251200/150‑1 (MQ=255)
        agaATGTCGGTTCCGGCCTGGCGAATCCAAGCAGCGCGGGAAAAGGTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCCTggg                                                                                                                                               <  1:110992/150‑1 (MQ=255)
         gaATGTCGGTTCCGGCCTGGCGAATCCAAGCAGCGCGGGAAAAGGTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCGGTGCggggg                                                                                                                                              >  1:4471556/1‑149 (MQ=255)
          aaTGTCGGTTCCGGCCTGGCGAATCCAAGCAGCGCGGGAAAAGGTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCCTGGGAc                                                                                                                                             <  2:6784419/150‑1 (MQ=255)
           aTGTCGGTTCCGGCCTGGCGAATCCAAGCAGCGCGGGAAAAGGTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCAGTGCAGGGTCa                                                                                                                                            >  1:6843732/1‑150 (MQ=255)
            tGTCGGTTCCGGCCTGGCGAATCCAAGCAGCGCGGGAAAAGGTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCCTGGGACAg                                                                                                                                           <  1:7223497/150‑1 (MQ=255)
             gTCGGTTCCGGCCTGGCGAATCCAAGCAGCGCGGGAAAAGGTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGGCGCCGTGCGGGGGCAGc                                                                                                                                          >  1:4280528/1‑150 (MQ=255)
             gTCGGTTCCGGCCTGGCGAATCCAAGCAGCGCGGGAAAAGGTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCCTGGGACAGc                                                                                                                                          <  2:6193477/150‑1 (MQ=255)
                ggTTCCGGCCTGGCGAATCCAAGCAGCGCGGGAAAAGGTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCCTGGGACAGCGGc                                                                                                                                       <  1:7836485/150‑1 (MQ=255)
                 gTTCCGGCCTGGCGAATCCAAGCAGCGCGGGAAAAGGTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCGGTCCGGGGACAGCGGcc                                                                                                                                      >  2:6961669/1‑149 (MQ=255)
                 gTTCCGGCCTGGCGAATCCAAGCAGCGCGGGAAAAGGTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCAGTCCTGGGAACGCGGCa                                                                                                                                      >  1:2407798/1‑150 (MQ=255)
                   tCCGGCCTGGCGAATCCAAGCAGCGCGGGAAAAGGTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCCTGGGACAGCGGCAAc                                                                                                                                    <  1:2158856/150‑1 (MQ=255)
                       gCCTGGCGAATCCAAGCAGCGCGGGAAAAGGTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCCTGGGACAGCGGCAACGGcc                                                                                                                                <  1:2879457/150‑1 (MQ=255)
                         cTGGCGAATCCAAGCAGCGCGGGAAAAGGTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCAGTCCGGGGACAGCGGCAACCGccag                                                                                                                              >  2:9734418/1‑148 (MQ=255)
                          tGGCGAATCCAAGCAGCGCGGGAAAAGGTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCCTGGGACAGCGGCAACGGCCTGa                                                                                                                             <  1:10166246/150‑1 (MQ=255)
                             cGAATCCAAGCAGCGCGGGAAAAGGTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCCTGGGACAGCGGCAACGGCCTGACGc                                                                                                                          <  1:9663647/150‑1 (MQ=255)
                               aaTCCAAGCAGCGCGGGAAAAGGTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGGCGCGGTGCGGGGGCCGCGGGCACCGCCGGACGcga                                                                                                                        >  1:5918900/1‑148 (MQ=255)
                                          cgcgGGAAAAGGTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCCTGGGACAGCGGCAACGGCCTGACGCTAACCGCCGAgcg                                                                                                             <  1:3125618/150‑1 (MQ=255)
                                           gcgGGAAATGGTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACCAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCGGTCCAGGGGCAGCCGCTACGGCGTGACGCTAACCGCCGAgggg                                                                                                            >  2:5880436/1‑147 (MQ=255)
                                            cgGGACAAGGTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCCTGGGCCATCGGCCACGGCCAGACCCGAAACGGCGAGcgcg                                                                                                           >  2:4703683/1‑150 (MQ=255)
                                             gggAAAAGGTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCCCAGTCCTGGGACAGCGGGAAAGGCCAGACCATAAACGCCCAGAGCgg                                                                                                          >  2:5381081/1‑150 (MQ=255)
                                              ggAAAAGGTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCCTGGGACAGCGGCAACGGCCTGACGCTAACCGCCGAGCGCggg                                                                                                         <  1:3190478/150‑1 (MQ=255)
                                              ggAAAAGGTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCCGGGCGGGGGCACCGGCAACCGCCCGACGCAAACCCCCCCGCGCggg                                                                                                         >  2:6495071/1‑150 (MQ=255)
                                              ggAAAAGGTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCCCGGTACTGGGCCAGCGGCCACCGCCTGAAGCGAACCGCCGGTCGCggg                                                                                                         >  2:3778137/1‑150 (MQ=255)
                                                 aaaGGTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGGCGCGGTCCGGGGACAGCGGCCACGGCCTGACGCAAAACCCCCAGCCCgggggc                                                                                                      >  1:10026583/1‑148 (MQ=255)
                                                 aaaGGTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGGCGCGGGGCGGGGGCAGCGGCAACCGCCAGAAGCGAAACCCCCCCCGCgggggc                                                                                                      >  1:3612944/1‑148 (MQ=255)
                                                 aaaGGTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGGCGCGGGACAGGGGCAGCGGCCACCGCCAGACGCGAACCGCCCCGCGCgggggc                                                                                                      >  1:5645270/1‑148 (MQ=255)
                                                 aaaGGTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCAAGGGCCAGCGGCACCCGCCTGACGCTACCCCCCCCGCGCgggggc                                                                                                      >  2:2344900/1‑148 (MQ=255)
                                                    ggTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCCTGGGACAGCGGCAACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTc                                                                                                   <  1:4955049/150‑1 (MQ=255)
                                                       ccGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCGGTGCGGGGACCGCGGCCACCGCCTGACGCGAACCGCCGCGCGCGGGGAccaccgg                                                                                                >  2:9029865/1‑145 (MQ=255)
                                                             cTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCCTGGGACAGCGGCAACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAAt                                                                                          <  1:1680748/150‑1 (MQ=255)
                                                              tGCATGCCGATTCCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCCTGGGACAGCGGCAACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATg                                                                                         <  2:10833984/150‑1 (MQ=255)
                                                              tGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCCTGGGACAGCGGCAACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATg                                                                                         <  1:8589471/150‑1 (MQ=255)
                                                               gCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCAGCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCCTGGGACAGCGGCAACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATgg                                                                                        <  2:3677219/150‑1 (MQ=255)
                                                               gCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCCTGGGACAGCGGCAACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATgg                                                                                        <  1:7398699/150‑1 (MQ=255)
                                                               gCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCCTGGGACAGCGGCAACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATgg                                                                                        <  1:2495051/150‑1 (MQ=255)
                                                               gCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCGGTGCTGGGGCCGCGGGCACGGCCGGTAGCTAACCGGCGAGCGGGGGGGCCGCAGGATCAAAgg                                                                                        >  2:8586134/1‑150 (MQ=255)
                                                               gCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCGGTCCTGGGACAGCGGCAACGGCCTGACGCAAACCGCCGAGCGCGGGGACCTCAGGATCAAAgg                                                                                        >  2:5407574/1‑150 (MQ=255)
                                                               gCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCGGGCCGGGGGCAGCGGCACCCGCCGGGCGCGAACCGCCGAGCGCGGGGCCCGCAGGGTAAAttg                                                                                        >  2:7596381/1‑148 (MQ=255)
                                                                 aTGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCCGGGGACCGCGGCAACCGCCAGACGCAAACCGCCCGGCGCGGGGACCTAAGGATCCAAGgcg                                                                                      >  2:9862935/1‑150 (MQ=255)
                                                                  tGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCCTGGGACAGCGGCAACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGgcgc                                                                                     <  2:8609097/150‑1 (MQ=255)
                                                                   gCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGGCGCCGTACAGGGACAGCGGCCACGGCCGGGGGCGAACCCCCGGGCGCGGGGACCACCGGATCCATGgcggg                                                                                    >  2:5611748/1‑148 (MQ=255)
                                                                   gCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCCTGGGACAGCGGCAACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGgcgcg                                                                                    <  1:6274723/150‑1 (MQ=255)
                                                                    ccGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCGGCGGTACAGGGGCACCGGCAACCGCCATTCGCGTACCGCCCAGCCCGGGGACCGAAGGATAAAAGgcgcgc                                                                                   >  2:1244214/1‑150 (MQ=255)
                                                                      gATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCCTGGGACAGCGGCAACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGGCGCGCTg                                                                                 <  1:1835413/150‑1 (MQ=255)
                                                                        tACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCCTGGGACAGCGGCAACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGGCGCGCTGAc                                                                               <  2:9369834/150‑1 (MQ=255)
                                                                        tACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCCGTCCGGGGGCAGCGGCCAAGGCCGGAAGCGAAACCCCCCGCGCGGGGGCCCCCAGCTAAAAGgcgcgcgggc                                                                               >  1:6145749/1‑146 (MQ=255)
                                                                         aCCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCCTGGGACAGCGGCAACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGGCGCGCTGACg                                                                              <  2:1003120/150‑1 (MQ=255)
                                                                            cTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCCCGGTGCGGGGACAGCGGCCACGGGCAGACGCGAACCCCCGCGCGCGGGGGCCAACGGATAAATGGCGCGCGGACGGcc                                                                           >  2:10655425/1‑150 (MQ=255)
                                                                                 ccGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCCCAGTACTGGGACAGGGGCCACGGGCCGACGCGAAACCCCGCGCGCGGGGCACGCATGATCAAATGCGCGCTGACGGcccccgg                                                                      >  2:10698482/1‑146 (MQ=255)
                                                                                   gCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGGCGCAGTGCATGGTCAGCGGCCACCGGCTGACGCAAAACCCCCAGCGCCGGGGCCACAGGATAAAAGGCGCCCCGACGGGCCCggggg                                                                    >  2:163636/1‑150 (MQ=255)
                                                                                    cAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGGCGGGGTGCGGGGGCCGCGGCCACCGGCGGTCGCAAAACCCCGCGCGGGGGGGCCACCCGGTAAAAGGCGGGCGGGCGGGCCCgggggg                                                                   >  2:2911004/1‑149 (MQ=255)
                                                                                    cAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCGGTCCTGGGACAGCGGCCACGGCCTGACGCGAACCGCCGCGCGCGGGGGCCTCAGGTACAATGGCGCGCGGACGGCCCCgggggg                                                                   >  2:9656188/1‑149 (MQ=255)
                                                                                     aaCCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCCTGGGACAGCGGCAACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGGCGCGCTGACGGCCCAGGGGGaa                                                                  <  2:4011286/150‑1 (MQ=255)
                                                                                       ccTTTCCACCAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCCTGGGACAGCGGCAACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGGCGCGCTGACGGCCCAGGGGGaaaa                                                                <  1:9597674/150‑1 (MQ=255)
                                                                                       ccTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCCTGGGACAGCGGCAACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGGCGCGCTGACGGCCCAGGGGGaaaa                                                                <  2:10888121/150‑1 (MQ=255)
                                                                                       ccTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCCTGGGACAGCGGCAACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGGCGCGCTGACGGCCCAGGGGGaaaa                                                                <  2:8121169/150‑1 (MQ=255)
                                                                                         tttCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGGGGTCCAGGGGCCCCGGGCACGGCCAGACGCTAAACGCCGGGGGCGGGGCCCGCAGGGTCAAAGGCGCGCTGAAGGCCCAGGGGGgaaaac                                                              >  2:2475720/1‑148 (MQ=255)
                                                                                         tttCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCAGGTCGGCGCGCCGCTGTCCTGGGACAGCGGCAACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGGCGCGCTGACGGCCCAGGGGGAAAACg                                                              <  2:449307/150‑1 (MQ=255)
                                                                                          ttCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTACTGGGACAACGGCCACCGCCAGACGCAAACCGCCCAGCGCGGGGAGCACAGGATAAATGGCGCGCTAACGGCCCAGGGGGCAAACGc                                                             >  2:4357074/1‑150 (MQ=255)
                                                                                           tCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCGGTACGGGGCCAACGGCAACCGCCAGAAGCACAACGCCCACCGCCGGGGCCAACTGATCAATTGGGCGCGGACGGCCCCCGGGGAAAAcccc                                                            >  2:6715720/1‑150 (MQ=255)
                                                                                            ccACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCCTGGGACAGCGGCAACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGGCGCGCTGACGGCCCAGGGGGAAAACGCCa                                                           <  2:3279217/150‑1 (MQ=255)
                                                                                             cacaAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCAGTCCGGGGGCAGCGGCAACCGCCGGACGCAAAACGCCGAGCGCGGGGCCCACAGGAGCAAATGCGCGCTGACGGCCCCGGGGGGAAAcccccc                                                          >  2:4260522/1‑148 (MQ=255)
                                                                                              acaAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCCTGGGACAGCGGCAACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGGCGCGCTGACGGCCCAGGGGGAAAACGCCAGc                                                         <  1:4405551/150‑1 (MQ=255)
                                                                                               caAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGGCGCGGGGCTGGGGCCGCGGGCACCGCCAGTCGCGAAACCCCCAGCGCGGGGGCCTCAGGGGCAAAGGCGCGCAGACCGCCCAGGGGGAAAACGCCAGcc                                                        >  2:1384272/1‑150 (MQ=255)
                                                                                               caAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCCTGGGACAGCGGCAACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGGCGCGCTGACGGCCCAGGGGGAAAACGCCAGcc                                                        <  2:7136848/150‑1 (MQ=255)
                                                                                               caAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCCTGGGACAGCGGCAACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGGCGCGCTGACGGCCCAGGGGGAAAACGCCAGcc                                                        <  2:6354945/150‑1 (MQ=255)
                                                                                               caAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCCTGGGACAGCGGCAACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGGCGCGCTGACGGCCCAGGGGGAAAACGCCAGcc                                                        <  1:802018/150‑1 (MQ=255)
                                                                                                   cAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCCTGGGACAGCGGCAACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGGCGCGCTGACGGCCCAGGGGGAAAACGCCAGCCTTAc                                                    <  1:8112545/150‑1 (MQ=255)
                                                                                                        tCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGGCGCGGTCCGGGGGCAGCGGCAACGGCCGGACGCTAACCGCCCAGCGCGGGGGCCGCCGGATCAAAGGCGCGCGGTCGGCCCCGGGGGAAAAAGCCCGCCATTCTcaca                                               >  2:1823849/1‑150 (MQ=255)
                                                                                                         cGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCCTGGGACAGCGGCAACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGGCGCGCTGACGGCCCAGGGGGAAAACGCCAGCCTTACTCTCaa                                              <  1:7301147/150‑1 (MQ=255)
                                                                                                         cGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCCTGGGACAGCGGCAACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGGCGCGCTGACGGCCCAGGGGGAAAACGCCAGCCTTACTCTCaa                                              <  1:10615582/150‑1 (MQ=255)
                                                                                                            aGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCCTGGGACAGCGGCAACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGGCGCGCTGACGGCCCAGGGGGAAAACGCCAGCCTTACTCTCAATGc                                           <  2:6151388/150‑1 (MQ=255)
                                                                                                              tGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCCTGGGACAGCGGCAACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGGCGCGCTGACGGCCCAGGGGGAAAACGCCAGCCTTACTCTCAATGCCg                                         <  1:6693195/150‑1 (MQ=255)
                                                                                                                 tCGCCACCCGGGGGAACGCGCATGTCGGCGCGCAGGTGTCGTGGTACAGCGGCAACGTCCTGAGGCTAACCGCGGAGCGCGGGGACCTCAGGATCAATGGCGCGCTGACGGCCCAGGGGGTTAACGCCAGCCTTACTCACAATGCCTGGc                                      <  1:50271/150‑1 (MQ=255)
                                                                                                                  cGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCCTGGGACAGCGGCAACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGGCGCGCTGACGGCCCAGGGGGAAAACGCCAGCCTTACTCTCAATGCCGGGCa                                     <  1:7726610/150‑1 (MQ=255)
                                                                                                                   gCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCCTGGGACAGCGGCAACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGGCGCGCTGACGGCCCAGGGGGAAAACGCCAGCCTTACTCTCAATGCCGGGCAg                                    <  1:4608542/150‑1 (MQ=255)
                                                                                                                   gCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCCTGGGACAGCGGCAACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGGCGCGCTGACGGCCCAGGGGGAAAACGCCAGCCTTACTCTCAATGCCGGGCAg                                    <  1:3031966/150‑1 (MQ=255)
                                                                                                                      aCCCGGGGCAACGCGCATGTCGGCGCGCCGCAGGCCAGGGCCAGCGGCAAAGGCCAGACGCAAACCGCCGAGCGCGGGGCCCTCAGGATCAAAGGGGCGCAGACGGCCCAGGGGGCTAACGCCAGCCATACTCACAATTCCGGGCAgcgc                                 >  2:11035828/1‑150 (MQ=255)
                                                                                                                        ccGGGGCAACGCGCATGTCGGCGCGCCGCTGTCCTGGGACAGCGGCAACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGGCGCGCTGACGGCCCAGGGGGAAAACGCCAGCCTTACTCTCAATGCCGGGCAGCGccc                               <  2:2107311/150‑1 (MQ=255)
                                                                                                                          ggggCAACGCGCATGTCGGCGCGGCGCAGTACAGGGACAGCGGCAAAGGGCATACCCTAAAACCCGCCCGCGGGGTCCACAGGAAAAATTGGGCGCGTAAGGCCCAGGGGGAAAAAGCCAACCAGAAAAGCAAATCCGGGCAGCGCActc                             >  2:7975327/1‑150 (MQ=255)
                                                                                                                           gggCAACGCGCATGTCGGCGCGCCGCTGTCCTGGGACAGCGGCAACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGGCGCGCTGACGGCCCAGGGGGAAAACGCCAGCCTTACTCTCAATGCCGGGCAGCGCCctct                            <  1:10900179/150‑1 (MQ=255)
                                                                                                                              cAACGCGCATGTCGGCGCGCCGCAGTCCGGGGTCCGCGGCCAAGGGCTGAAGCTAACCCCCGAGGGCGGGGGCCTCCCGAGAAAATGGGCGCTGTATGCCCCGGGGGGTAAAGCCAGGCTTACTCGAAATTGCGGGCCGCGGCCACACCg                         >  2:839029/1‑150 (MQ=255)
                                                                                                                                 cgcgCATGTCGGCGCGCCGCTGTCCTGGGACAGCGGCAACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGGCGCGCTGACGGCCCAGGGGGAAAACGCCAGCCTTACTCTCAATGCCGGGCAGCGCCCTCTCCGTAt                      <  2:7397950/150‑1 (MQ=255)
                                                                                                                                 cgcgCATGTCGGCGCGCCGCTGTCCTGGGACAGCGGCAACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGGCGCGCTGACGGCCCAGGGGGAAAACGCCAGCCTTACTCTCAATGCCGGGCAGCGCCCTCTCCGTAt                      <  1:2818323/150‑1 (MQ=255)
                                                                                                                                    gcATGTCGGCGCGCCGCTGTCCTGGGACAGCGGCAACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGGCGCGCTGACGGCCCAGGGGGAAAACGCCAGCCTTACTCTCAATGCCGGGCAGCGCCCTCTCCGTATcga                   <  1:1961180/150‑1 (MQ=255)
                                                                                                                                     cATGTCGGCGCGCCGCTGTCCTGGGACAGCGGCAACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGGCGCGCTGACGGCCCAGGGGGAAAACGCCAGCCTTACTCTCAATGCCGGGCAGCGCCCTCTCCGTATcgac                  <  1:658244/150‑1 (MQ=255)
                                                                                                                                         tCGGCGCGCCGCTGTCCTGGGACAGCGGCAACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGGCGCGCTGACGGCCCAGGGGGAAAACGCCAGCCTTACTCTCAATGCCGGGCAGCGCCCTCTCCGTATCGACGACa              <  1:3986823/150‑1 (MQ=255)
                                                                                                                                           ggCGCGCCGCTGTCCGGGGACAGCGGCAACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGGCGCGCTGACGGCCCAGGGGGAAAACGCCAGCCTTACTCTCAATGCCGGGCAGCGCCCTCTCCGTATCGACGACAGc            <  1:4714140/150‑1 (MQ=255)
                                                                                                                                                gccgcTGTCCTGGGACAGCGGCAACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGGCGCGCTGACGGCCCAGGGGGAAAACGCCAGCCTTACTCTCAATGCCGGGCAGCGCCCTCTCCGTATCGACGACAGCctctc       <  1:6069718/150‑1 (MQ=255)
                                                                                                                                                 ccgcTGTCCTGGGACAGCGGCAACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGGCGCGCTGACGGCCCAGGGGGAAAACGCCAGCCTTACTCTCAATGCCGGGCAGCGCCCTCTCCGTATCGACGACAGCctctct      >  1:8865625/1‑150 (MQ=255)
                                                                                                                                                 ccgcTGTCCTGGGACAGCGGCAACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGGCGCGCTGACGGCCCAGGGGGAAAACGCCAGCCTTACTCTCAATGCCGGGCAGCGCCCTCTCCGTATCGACGACAGCctctct      >  1:542975/1‑150 (MQ=255)
                                                                                                                                                 ccgcTGTCCTGGGACAGCGGCAACGGCCTGACGCTAACCGCCGAGCGCGGGGACCACAGGATCAATGGCGCGATGACGGCCCAGGGGGAAAACGCCAGCCTTACTCTCAATGCCGGGCAGCGCCCTCTCCGTATCGACGACCGCcactct      >  2:1275431/1‑150 (MQ=255)
                                                                                                                                                  cgcTGTCCTGGGACAGCGGCAACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGGCGCGCTGACGGCCCAGGGGGAAAACGCCAGCCTTACTCTCAATGCCGGGCAGCGCCCTCTCCGTATCGACGACAGCctctctc     <  2:5037475/150‑1 (MQ=255)
                                                                                                                                                     tGTCCTGGGACAGCGGCAACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGGCGCGCTGACGGCCCAGGGGGAAAACGCCAGCCTTACTCTCAATGCCGGGCAGCGCCCTCTCCGTATCGACGACAGCCTCTCTCTCa  <  2:10528132/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GAGCAGCGAGAATGTCGGTTCCGGCCTGGCGAATCCAAGCAGCGCGGGAAAAGGTCCGACGCTGCATGCCGATACCCTGGCCCGCAACCTTTCCACAAGCAACGTCGAAGTGGTCGCCACCCGGGGCAACGCGCATGTCGGCGCGCCGCTGTCCTGGGACAGCGGCAACGGCCTGACGCTAACCGCCGAGCGCGGGGACCTCAGGATCAATGGCGCGCTGACGGCCCAGGGGGAAAACGCCAGCCTTACTCTCAATGCCGGGCAGCGCCCTCTCCGTATCGACGACAGCCTCTCTCTCA  >  NC_008463/5248801‑5249099

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑