Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 3,424,199 A→G Q436Q (CAA→CAG)  mexY → multidrug efflux RND transporter permease subunit MexY

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084633,424,1990AG100.0% 1782.8 / NA 460Q436Q (CAA→CAG) mexYmultidrug efflux RND transporter permease subunit MexY
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (265/195);  total (265/195)

Only 100 of 460 total aligned reads displayed.

CTCGATCAACGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCCAAGAAGGCCTGTCGCCGCACGACACCACGGTCAAGGCGATGCGCCAAATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTACCGCCAGTTCGCGGTGACCCTGGCGGTCTCCATCGGCTTCTCGGCGTTCCTCGCGCT  >  NC_008463/3424052‑3424345
                                                                                                                                                   |                                                                                                                                                  
 tcGATCAACGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATc                                                                                                                                                 >  1:4301518/1‑150 (MQ=255)
  cGATCAACGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCa                                                                                                                                                >  1:5429260/1‑150 (MQ=255)
  cGATCAACGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCa                                                                                                                                                <  1:2369453/150‑1 (MQ=255)
           tGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCa                                                                                                                                       >  1:10671089/1‑150 (MQ=255)
            gCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCAt                                                                                                                                      >  2:7778099/1‑150 (MQ=255)
               gACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCAtcgt                                                                                                                                   <  1:10443920/150‑1 (MQ=255)
               gACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCAtcgt                                                                                                                                   >  2:6903126/1‑150 (MQ=255)
                aCCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCAtcgtc                                                                                                                                  <  2:2964677/150‑1 (MQ=255)
                   aTGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCAGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGc                                                                                                                               >  2:6614618/1‑150 (MQ=21)
                    tGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCa                                                                                                                              >  1:2454390/1‑150 (MQ=255)
                     gTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCAt                                                                                                                             <  1:1350286/150‑1 (MQ=255)
                     gTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCAt                                                                                                                             >  2:1976311/1‑150 (MQ=255)
                     gTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCAt                                                                                                                             <  1:6442557/150‑1 (MQ=255)
                       tCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCa                                                                                                                           >  2:10303827/1‑150 (MQ=255)
                       tCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCa                                                                                                                           >  2:9806100/1‑150 (MQ=255)
                         ggCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCAcc                                                                                                                         >  2:4367425/1‑150 (MQ=255)
                              ggTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCgtggt                                                                                                                    <  2:1268366/150‑1 (MQ=255)
                                tCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGc                                                                                                                  >  1:3939997/1‑150 (MQ=255)
                                    ggCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGt                                                                                                              <  1:1624645/150‑1 (MQ=255)
                                    ggCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGt                                                                                                              <  1:3303859/150‑1 (MQ=255)
                                       gATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGtctc                                                                                                           >  2:536964/1‑150 (MQ=255)
                                        aTCGGCATCCTGGTGGACGACGCGATCATCGTGGTTGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTATCg                                                                                                          <  2:5778671/150‑1 (MQ=25)
                                         tCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCgg                                                                                                         >  2:10694667/1‑150 (MQ=255)
                                         tCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCgg                                                                                                         >  1:4143286/1‑150 (MQ=255)
                                           ggCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTa                                                                                                       >  2:9320903/1‑150 (MQ=255)
                                            gCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTAt                                                                                                      >  1:5335102/1‑150 (MQ=255)
                                            gCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGAGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTAt                                                                                                      >  2:4014966/1‑150 (MQ=25)
                                                 cTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTg                                                                                                 >  2:134045/1‑150 (MQ=255)
                                                  tggtggACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGc                                                                                                >  1:6920178/1‑150 (MQ=255)
                                                  tggtggACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGc                                                                                                >  1:7859866/1‑150 (MQ=255)
                                                   ggtggACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGcc                                                                                               >  1:2558591/1‑150 (MQ=255)
                                                    gtggACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCg                                                                                              >  2:8566517/1‑150 (MQ=255)
                                                      ggACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCACGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGATGGTCTCGGTATTCGTGCCGAt                                                                                            <  1:6224458/150‑1 (MQ=38)
                                                      ggACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGAt                                                                                            >  1:1157209/1‑150 (MQ=255)
                                                        acgacgCGATCATCGTGGTGGAGCACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCCTCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATgg                                                                                          >  2:6329263/1‑150 (MQ=38)
                                                        acgacgCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATgg                                                                                          >  2:5022431/1‑150 (MQ=255)
                                                          gacgCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCg                                                                                        >  1:3800480/1‑150 (MQ=255)
                                                            cgcgATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGtt                                                                                      <  1:8958570/150‑1 (MQ=255)
                                                             gcgATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGttc                                                                                     >  2:7043199/1‑150 (MQ=255)
                                                              cgATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGttct                                                                                    <  2:2454971/150‑1 (MQ=255)
                                                                atcatcGTGGTGGAGAACGTCGAGCGGCTGCTGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGttcttc                                                                                  <  2:10010061/150‑1 (MQ=38)
                                                                 tcatcGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCa                                                                                 >  1:9512786/1‑150 (MQ=255)
                                                                  catcGGGGTGGAGAACGTCGAGCGGCTGATGGCCGCGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCGCGGTATTCGTGCCGCTGGCGAGCTTCAg                                                                                >  2:5650535/1‑150 (MQ=255)
                                                                   atcGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAgc                                                                               <  1:3416962/150‑1 (MQ=255)
                                                                   atcGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAgc                                                                               <  2:10841843/150‑1 (MQ=255)
                                                                    tcGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAgcg                                                                              >  1:6949548/1‑150 (MQ=255)
                                                                    tcGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAgcg                                                                              <  2:2992208/150‑1 (MQ=255)
                                                                    tcGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAgcg                                                                              >  1:975583/1‑150 (MQ=255)
                                                                     cGTGGTGGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAgcgg                                                                             <  2:9430949/150‑1 (MQ=255)
                                                                      gtggagGAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCAGTCGCAGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCAACGACGGCCTCACCGAGGGGCTGGTCTCGGTAATCGTGCCGAAGGCGAACTTCAgcggc                                                                            >  2:7265047/1‑150 (MQ=255)
                                                                        ggtggAGAACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGgcgc                                                                          >  1:10167068/1‑150 (MQ=255)
                                                                            gagaACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTg                                                                      >  1:6526818/1‑150 (MQ=255)
                                                                             agaACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTgg                                                                     <  1:1811199/150‑1 (MQ=255)
                                                                             agaACGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTgg                                                                     <  1:4443026/150‑1 (MQ=255)
                                                                               aaCGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGAGTTCTTCAGCGGCGCGGTGGGc                                                                   >  2:2095847/1‑150 (MQ=38)
                                                                                 cGTCGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCaa                                                                 <  1:3125490/150‑1 (MQ=255)
                                                                                    cGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACAt                                                              <  2:10668672/150‑1 (MQ=255)
                                                                                    cGAGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACAt                                                              >  1:8637484/1‑150 (MQ=255)
                                                                                      aGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCt                                                            >  1:1531678/1‑150 (MQ=255)
                                                                                      aGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCt                                                            >  1:5863451/1‑150 (MQ=255)
                                                                                      aGCGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCt                                                            >  2:2248830/1‑150 (MQ=255)
                                                                                        cGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTAc                                                          <  2:7454164/150‑1 (MQ=255)
                                                                                        cGGCTGATGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTAc                                                          <  1:6666790/150‑1 (MQ=255)
                                                                                              aTGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTACCGCCAg                                                    <  1:8223616/150‑1 (MQ=255)
                                                                                               tGGCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTACCGCCAGt                                                   >  1:10892195/1‑150 (MQ=255)
                                                                                                ggCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTACCGCCAGtt                                                  >  1:721738/1‑150 (MQ=255)
                                                                                                ggCCGAGGAAGGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTACCGCCAGtt                                                  <  1:5197680/150‑1 (MQ=255)
                                                                                                  ccgaggaagGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTACCGCCAGTTcg                                                <  1:10366126/145‑1 (MQ=255)
                                                                                                   cgaggaagGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGTGGCCATCGTCGGCATAACCGTGGAGCTGGTCTCGGTATTCGAGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTACCGCCAGTTcgc                                               >  2:3519180/5‑150 (MQ=255)
                                                                                                   cgaggaagGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTACCGCCAGTTcgc                                               >  1:369911/5‑150 (MQ=255)
                                                                                                     aggaagGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTACCGCCAGTTCGCgg                                             >  2:8926891/3‑150 (MQ=255)
                                                                                                     aggaagGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTACCGCCAGTTCGCgg                                             >  1:4595665/3‑150 (MQ=255)
                                                                                                      ggaagGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTACCGCCAGTTCGCGGt                                            <  2:608458/149‑1 (MQ=255)
                                                                                                        aagGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTACCGCCAGTTCGCGGTGa                                          >  2:1539889/1‑150 (MQ=255)
                                                                                                         agGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTACCGCCAGTTCGCGGTGAc                                         <  2:2630155/150‑1 (MQ=255)
                                                                                                         agGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTACCGCCAGTTCGCGGTGAc                                         >  1:9195263/1‑150 (MQ=255)
                                                                                                         agGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTACCGCCAGTTCGCGGTGAc                                         >  2:3770485/1‑150 (MQ=255)
                                                                                                         agGCCTGTCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTACCGCCAGTTCGCGGTGAc                                         >  1:2089458/1‑150 (MQ=255)
                                                                                                                tCGCCGCACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTACCGCCAGTTCGCGGTGACCCTGGCg                                  >  2:450087/1‑150 (MQ=255)
                                                                                                                  gccgcACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTACCGCCAGTTCGCGGTGACCCTGGCGGt                                <  1:10035435/150‑1 (MQ=255)
                                                                                                                  gccgcACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTACCGCCAGTTCGCGGTGACCCTGGCGGt                                >  1:9360364/1‑150 (MQ=255)
                                                                                                                      cACGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTACCGCCAGTTCGCGGTGACCCTGGCGGTCTcc                            >  2:4696784/1‑150 (MQ=255)
                                                                                                                        cGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTACCGCCAGTTCGCGGTGACCCTGGCGGTCTCCAt                          <  2:1047408/150‑1 (MQ=255)
                                                                                                                        cGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTACCGCCAGTTCGCGGTGACCCTGGCGGTCTCCAt                          >  2:4108458/1‑150 (MQ=255)
                                                                                                                        cGACGCCACGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTACCGCCAGTTCGCGGTGACCCTGGCGGTCTCCAt                          <  2:10155785/150‑1 (MQ=255)
                                                                                                                          acgccacGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTACCGCCAGTTCGCGGTGACCCTGGCGGTCTCCATCg                        >  2:1759061/4‑150 (MQ=255)
                                                                                                                            gccacGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTACCGCCAGTTCGCGGTGACCCTGGCGGTCTCCATCGGc                      <  1:6107128/149‑1 (MQ=255)
                                                                                                                               acGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTACCGCCAGTTCGCGGTGACCCTGGCGGTCTCCATCGGCTtc                   >  1:5923205/1‑150 (MQ=255)
                                                                                                                                cGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTACCGCCAGTTCGCGGTGACCCTGGCGGTCTCCATCGGCTtct                  <  2:6677412/150‑1 (MQ=255)
                                                                                                                                cGGTCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTACCGCCAGTTCGCGGTGACCCTGGCGGTCTCCATCGGCTtct                  <  2:8041863/150‑1 (MQ=255)
                                                                                                                                   tCAAGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTACCGCCAGTTCGCGGTGACCCTGGCGGTCTCCATCGGCTTCTCgg               <  2:3280298/150‑1 (MQ=255)
                                                                                                                                      aGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTACCGCCAGTTCGCGGTGACCCTGGCGGTCTCCATCGGCTTCTCGGCGt            >  1:4711550/1‑150 (MQ=255)
                                                                                                                                      aGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTACCGCCAGTTCGCGGTGACCCTGGCGGTCTCCATCGGCTTCTCGGCGt            >  1:4923879/1‑150 (MQ=255)
                                                                                                                                      aGGCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGAAGGCGTTCTTCAGCGGCGCGGTGGGCCACATCAACCGCCAGTTCGCGGTGACCCTGGCGGTCTCCATCGGCTTCTCGGCGt            >  2:1715512/1‑150 (MQ=255)
                                                                                                                                        gCGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTACCGCCAGTTCGCGGTGACCCTGGCGGTCTCCATCGGCTTCTCGGCGTTc          <  1:4094016/150‑1 (MQ=255)
                                                                                                                                         cGATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTACCGCCAGTTCGCGGTGACCCTGGCGGTCTCCATCGGCTTCTCGGCGTTcc         <  2:10983904/150‑1 (MQ=255)
                                                                                                                                          gATGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTACCGCCAGTTCGCGGTGACCCTGGCGGTCTCCATCGGCTTCTCGGCGTTCCt        <  1:7399859/150‑1 (MQ=255)
                                                                                                                                            tGCGCCAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTACCGCCAGTTCGCGGTGACCCTGGCGGTCTCCATCGGCTTCTCGGCGTTCCTcg      >  1:5090412/1‑150 (MQ=255)
                                                                                                                                                ccAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTACCGCCAGTTCGCGGTGACCCTGGCGGTCTCCATCGGCTTCTCGGCGTTCCTCGCGCt  >  1:355503/1‑150 (MQ=255)
                                                                                                                                                ccAGATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTACCGCCAGTTCGCGGTGACCCTGGCGGTCTCCATCGGCTTCTCGGCGTTCCTCGCGCt  >  2:9060242/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CTCGATCAACGTGCTGACCATGTTCGGCATGGTCCTGGCGATCGGCATCCTGGTGGACGACGCGATCATCGTGGTGGAGAACGTCGAGCGGCTGATGGCCCAAGAAGGCCTGTCGCCGCACGACACCACGGTCAAGGCGATGCGCCAAATCAGCGGGGCCATCGTCGGCATCACCGTGGTGCTGGTCTCGGTATTCGTGCCGATGGCGTTCTTCAGCGGCGCGGTGGGCAACATCTACCGCCAGTTCGCGGTGACCCTGGCGGTCTCCATCGGCTTCTCGGCGTTCCTCGCGCT  >  NC_008463/3424052‑3424345

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑