Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 3,566,769 A→G pseudogene (1120/1275 nt) PA14_RS16215 → fatty acid desaturase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084633,566,7690AG100.0% 1820.0 / NA 467pseudogene (1120/1275 nt)PA14_RS16215fatty acid desaturase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (222/245);  total (222/245)

Only 100 of 469 total aligned reads displayed.

GTTCTCCTTCATGCAGGACCTGCCCAGCCACGACTTCCACCACCCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACAAGGCGCGCCCCTCAAAGTTCGGCCCGATGACCAAAACCTGGGGCATGTGGGAAAACTTCCTGATCCTGCGCAACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGG‑A‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCCGCCAACCAGCCCCACACCAACCAACCCGCATCCCAGGC  >  NC_008463/3566621‑3566914
                                                                                                                                                    |                                                                                                                                                   
ggtctcCTTCCTGCAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGagaa                                                                                                                                                   <  2:9124538/148‑1 (MQ=255)
  tctcCTTCATGCAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACt                                                                                                                                                 <  1:10721176/150‑1 (MQ=255)
   ctcCTTCATGCAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACtt                                                                                                                                                >  2:3731508/1‑150 (MQ=255)
     ccTTCATGCAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTcc                                                                                                                                              <  1:5490327/150‑1 (MQ=255)
      cTTCATGCAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGACGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCt                                                                                                                                             >  2:691337/1‑150 (MQ=255)
       ttCATGCAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTg                                                                                                                                            <  2:1966306/150‑1 (MQ=255)
       ttCATGCAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTg                                                                                                                                            >  2:217568/1‑150 (MQ=255)
         cATGCAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGAt                                                                                                                                          <  2:7569149/150‑1 (MQ=255)
             cAGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTg                                                                                                                                      >  1:4246714/1‑150 (MQ=255)
              aGGACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTgc                                                                                                                                     >  1:3915211/1‑150 (MQ=255)
               ggACCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTgcg                                                                                                                                    >  2:2669068/1‑150 (MQ=255)
                 aCCTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTgcgcg                                                                                                                                  >  2:6746214/1‑149 (MQ=255)
                   cTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGac                                                                                                                                <  1:11048122/150‑1 (MQ=255)
                   cTGCCCAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGac                                                                                                                                <  2:7620236/150‑1 (MQ=255)
                       ccAGCCACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGaccacc                                                                                                                            >  1:236148/1‑150 (MQ=255)
                           ccACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTg                                                                                                                        >  1:8767537/1‑150 (MQ=255)
                            cACGACTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGc                                                                                                                       <  1:521047/150‑1 (MQ=255)
                                aCTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCcgcg                                                                                                                   <  1:6866632/150‑1 (MQ=255)
                                 cTTCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCgg                                                                                                                  <  2:397814/150‑1 (MQ=255)
                                  ttCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGc                                                                                                                 <  2:1543947/150‑1 (MQ=255)
                                  ttCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGc                                                                                                                 >  1:9719994/1‑150 (MQ=255)
                                  ttCCACCA‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGc                                                                                                                 >  1:10896271/1‑150 (MQ=255)
                                    ccacca‑CCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCa                                                                                                               <  2:8362040/150‑1 (MQ=255)
                                     cacca‑cCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAg                                                                                                              >  1:7531075/1‑150 (MQ=255)
                                       accaccGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGt                                                                                                             <  1:4985049/146‑1 (MQ=255)
                                       accaccGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGt                                                                                                             <  2:4320089/146‑1 (MQ=255)
                                        ccaccGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGTCCGAGACCTGGGGCATGTGGGCGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTc                                                                                                            >  2:8579982/4‑150 (MQ=255)
                                         caccGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCg                                                                                                           >  1:2581066/3‑150 (MQ=255)
                                             gCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGAcc                                                                                                       <  1:5713925/150‑1 (MQ=255)
                                              cAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGACGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGAccc                                                                                                      <  2:844716/150‑1 (MQ=255)
                                               caGCCGGGGGTGAACTACTCGCGCATCGCGCCGGAGCGCGCGGCCACCGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGTCCAGACGGACCCg                                                                                                     <  2:2102847/149‑1 (MQ=255)
                                               aaGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCg                                                                                                     <  1:7880176/150‑1 (MQ=255)
                                                aGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGt                                                                                                    >  1:1039003/1‑150 (MQ=255)
                                                aGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGACCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGt                                                                                                    >  2:2383286/1‑150 (MQ=255)
                                                 gCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGtt                                                                                                   <  1:9387404/150‑1 (MQ=255)
                                                  ccGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTc                                                                                                  <  2:6237979/150‑1 (MQ=255)
                                                   cGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCg                                                                                                 <  2:50611/150‑1 (MQ=255)
                                                    gggggTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCgg                                                                                                >  2:108427/1‑150 (MQ=255)
                                                     ggggTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGG‑a                                                                                              >  2:1677710/1‑150 (MQ=255)
                                                     ggggTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGG‑a                                                                                              <  1:2167040/150‑1 (MQ=255)
                                                      gggTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGGTCGG‑aa                                                                                             >  1:4647820/1‑149 (MQ=255)
                                                       ggTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGG‑aat                                                                                            <  2:3117278/150‑3 (MQ=255)
                                                       ggTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGG‑aat                                                                                            <  2:8687869/150‑3 (MQ=255)
                                                       ggTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGG‑aat                                                                                            <  1:2706031/150‑3 (MQ=255)
                                                          gAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGG‑aatcgt                                                                                         >  2:8778445/1‑145 (MQ=255)
                                                                      cATCGCGCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACcg                                                                             >  1:4082884/1‑150 (MQ=255)
                                                                         cgcgCCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCga                                                                          >  2:3210448/1‑150 (MQ=255)
                                                                          gcgcCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCgag                                                                         >  1:349308/1‑150 (MQ=255)
                                                                           cgcCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCgaga                                                                        >  2:10435941/1‑150 (MQ=255)
                                                                            gcCGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGaa                                                                       <  2:52984/150‑1 (MQ=255)
                                                                             ccGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAAc                                                                      <  2:10607486/150‑1 (MQ=255)
                                                                             ccGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAAc                                                                      >  1:1335649/1‑150 (MQ=255)
                                                                              cGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAAcc                                                                     >  1:1718853/1‑150 (MQ=255)
                                                                              cGGAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAAcc                                                                     >  1:679383/1‑150 (MQ=255)
                                                                               ggAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCa                                                                    <  1:9917660/150‑1 (MQ=255)
                                                                               ggAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCa                                                                    >  2:7330001/1‑150 (MQ=255)
                                                                                gAGCGCGCGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCAt                                                                   <  1:674705/150‑1 (MQ=255)
                                                                                  gcgcgcGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGc                                                                 >  2:8023618/1‑150 (MQ=255)
                                                                                  gcgcgcGGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGc                                                                 <  1:8285224/150‑1 (MQ=255)
                                                                                   cgcgcgGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGcc                                                                <  1:3750834/150‑1 (MQ=255)
                                                                                    gcgcgGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGccc                                                               >  2:8955802/1‑150 (MQ=255)
                                                                                    gcgcgGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGccc                                                               <  2:2268831/150‑1 (MQ=255)
                                                                                    gcgcgGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGccc                                                               <  2:5447237/150‑1 (MQ=255)
                                                                                       cgGCCAACGAGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCAGGGGCATGTGGGAGAACTGCCAGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCCTGCCCGgc                                                            <  2:9666434/150‑1 (MQ=255)
                                                                                           caacgaGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGgctcgc                                                        >  1:2828276/1‑150 (MQ=255)
                                                                                             acgaGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGgctcgctc                                                      <  2:1260659/147‑1 (MQ=255)
                                                                                             acgaGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGgctcgctc                                                      >  2:9254296/4‑150 (MQ=255)
                                                                                                aGGCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCgg                                                   <  2:7284025/150‑1 (MQ=255)
                                                                                                 ggCGCGCCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGga                                                  >  1:6089814/1‑150 (MQ=255)
                                                                                                    gcgcCCCTCGCAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGAc                                               <  2:4523050/150‑1 (MQ=255)
                                                                                                    gcgcCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGAc                                               <  1:6987212/150‑1 (MQ=255)
                                                                                                    gcgcCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGAc                                               <  2:10774338/150‑1 (MQ=255)
                                                                                                    gcgcCCCTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGAc                                               <  2:4903467/150‑1 (MQ=255)
                                                                                                         ccTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATg                                          >  2:10977463/1‑150 (MQ=255)
                                                                                                          cTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATgc                                         <  1:8625974/150‑1 (MQ=255)
                                                                                                          cTCGAAGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATgc                                         <  2:4218861/150‑1 (MQ=255)
                                                                                                           tcgaaGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATgcc                                        <  1:10012671/147‑1 (MQ=255)
                                                                                                           tcgaaGTTCGGCCCGATGACCGAGACCTGGGGCACGTGGGAGACCTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGG‑ACTCGTAGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATgcc                                        <  2:1399783/147‑1 (MQ=255)
                                                                                                            cgaaGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATgccg                                       >  1:9092667/3‑150 (MQ=255)
                                                                                                             caaGTTCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATgccgc                                      <  1:840267/149‑1 (MQ=255)
                                                                                                                 ttCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCCGCCAAc                                  >  2:4382693/1‑150 (MQ=255)
                                                                                                                  tCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCCGCCAAcc                                 >  1:10148989/1‑150 (MQ=255)
                                                                                                                  tCGGCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACAGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCCGCCAAcc                                 >  2:6362325/1‑150 (MQ=255)
                                                                                                                     gCCCGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCCGCCAACCAGc                              >  2:10319947/1‑150 (MQ=255)
                                                                                                                       ccGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCCGCCAACCAGccc                            >  2:6868472/1‑150 (MQ=255)
                                                                                                                       ccGATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCCGCCAACCAGccc                            <  1:3676032/150‑1 (MQ=255)
                                                                                                                         gATGACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCCGCCAACCAGCCCca                          <  2:5874725/150‑1 (MQ=255)
                                                                                                                            gACCGAGACCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCCGCCAACCAGCCCCacac                       >  1:2425694/1‑150 (MQ=255)
                                                                                                                             cccgagaCCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCCGCCAACCAGCCCCACacc                      <  1:5856846/144‑1 (MQ=255)
                                                                                                                                  gaCCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCCGCCAACCAGCCCCACaccaacca                 >  2:3624424/2‑150 (MQ=255)
                                                                                                                                   aCCTGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCTCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCCGCCAACCAGCCCCACAccaaccaa                >  2:3935481/1‑150 (MQ=255)
                                                                                                                                      tGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCCGCCAACCAGCCCCACACCAACCAAccc             >  2:4735565/1‑150 (MQ=255)
                                                                                                                                      tGGGGCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCCGCCAACCAGCCCCACACCAACCAAccc             <  1:6531874/150‑1 (MQ=255)
                                                                                                                                       ggggCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCCGCCAACCAGCCCCACACCAACCAACCCg            <  2:345334/150‑1 (MQ=255)
                                                                                                                                         ggCATGTGGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCCGCCAACCAGCCCCACACCAACCAACCCGCa          <  1:9659604/150‑1 (MQ=255)
                                                                                                                                             tgtgGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCAGGCTCGCTCCGGAGACCGATGCCGCCAACCAGCCCCACACCAACCAACCCGCATccc      <  1:3020226/150‑1 (MQ=255)
                                                                                                                                               tgGGAGAACTTCCTGATCCTGCTCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCCGCCAACCAGCCCCACACCAACCAACCCGCATCCCAg    <  2:110702/150‑1 (MQ=255)
                                                                                                                                               tgGGAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCCGCCAACCAGCCCCACACCAACCAACCCGCATCCCAg    >  2:3344349/1‑150 (MQ=255)
                                                                                                                                               tgGGAGAACGTCCAGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCCGCCAACCAGCCCCACACCAACCAACCCGCATCCCAg    <  2:5707675/150‑1 (MQ=255)
                                                                                                                                                gggAGAACTTCCTGATCCTGCGCGACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGGAA‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCCGCCAACCAGCCCCACACCAACCAACCCGCATCCCAgg   >  2:7057840/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GTTCTCCTTCATGCAGGACCTGCCCAGCCACGACTTCCACCACCCGCAAGCCGGGGGTGAACTTCTGGCGCATCGCGCCGGAGCGCGCGGCCAACAAGGCGCGCCCCTCAAAGTTCGGCCCGATGACCAAAACCTGGGGCATGTGGGAAAACTTCCTGATCCTGCGCAACCACCTCTGCCGCGGCCAGTCGGACCCGTTCGG‑A‑TCGTCGACTGGTACCGCGAGAACCATGCCCGGCTCGCTCCGGAGACCGATGCCGCCAACCAGCCCCACACCAACCAACCCGCATCCCAGGC  >  NC_008463/3566621‑3566914

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑