Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 3,769,180 C→G A44A (GCG→GCC)  pscL ← SctL family type III secretion system stator protein PscL

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084633,769,1800CG100.0% 1977.7 / NA 514A44A (GCG→GCC) pscLSctL family type III secretion system stator protein PscL
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (280/234);  total (280/234)

Only 100 of 533 total aligned reads displayed.

GCGATCGACCTGCCGGTAGTAGCGATTGCAGCGCAGTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCCGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTCAACAAATGGAAGCATTCATCGCTCGCTTGT  >  NC_008463/3769033‑3769326
                                                                                                                                                   |                                                                                                                                                  
gCGATCGACCTGCCGGTAGTAGCGATTGCAGCGCAGTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCggc                                                                                                                                                  >  2:2138790/1‑150 (MQ=255)
gCGATCGACCTGCCGGTAGTAGCGATTGCAGCGCAGTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCggc                                                                                                                                                  >  1:1200038/1‑150 (MQ=255)
 cGATCGACCTGCCGGTAGTAGCGATTGCAGCGCAGTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCggcg                                                                                                                                                 >  1:7697119/1‑150 (MQ=255)
  gATCGACCTGCCGGTAGTAGCGATTGCAGCGCAGTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTcggcgg                                                                                                                                                >  1:6483847/1‑150 (MQ=255)
       aCCTGCCGGTAGTAGCGATTGCAGCGCAGTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGc                                                                                                                                           >  1:10316842/1‑150 (MQ=255)
       aCCTGCCGGTAGTAGCGATTGCAGCGCAGTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGc                                                                                                                                           >  2:3926826/1‑150 (MQ=255)
        ccTGCCGGTAGTAGCGATTGCAGCGCAGTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGcg                                                                                                                                          >  1:3747111/1‑149 (MQ=255)
        ccTGCCGGTAGTAGCGATTGCAGCGCAGTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCt                                                                                                                                          <  2:815597/150‑1 (MQ=255)
         cTGCCGGTAGTAGCGATTGCAGCGCAGTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGcac                                                                                                                                         >  1:3091386/1‑148 (MQ=255)
         cTGCCGGTAGTAGCGATTGCAGCGCAGTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTc                                                                                                                                         >  1:7179671/1‑150 (MQ=255)
           gCCGGTAGTAGCGATTGCAGCGCAGTAGCGTTTCCAGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTcgc                                                                                                                                       <  2:1172412/150‑1 (MQ=255)
            ccGGTAGTAGCGATTGCAGCGCAGTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTcgcg                                                                                                                                      >  2:7718801/1‑150 (MQ=255)
             cGGTAGTAGCGCTTGCAGCGCAGTAGCGTTTCCGGGATCAGCCCGGCCGGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTTAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGt                                                                                                                                     <  2:5687077/150‑1 (MQ=255)
               gtagtaGCGATTGCAGCGCAGTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGc                                                                                                                                   <  2:8716239/150‑1 (MQ=255)
                       gATTGCAGCGCAGTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCCGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTcca                                                                                                                           >  1:2296994/1‑150 (MQ=255)
                       gATTGCAGCGCAGTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTcca                                                                                                                           >  2:2982884/1‑150 (MQ=255)
                         ttGCAGCGCAGTAGCGTTTCCTGGCTCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTGCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATGTCGGCGGCACGCTCGCGTGCCGCTTccacc                                                                                                                         >  2:4322268/1‑150 (MQ=255)
                           gCAGCGCAGTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAg                                                                                                                       <  2:7905543/150‑1 (MQ=255)
                              gcgcAGTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCg                                                                                                                    >  1:3416739/1‑150 (MQ=255)
                               cgcAGTCGCGTTTCCAGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCgg                                                                                                                   <  1:8540439/150‑1 (MQ=255)
                                 cAGTAGCGTGTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGtt                                                                                                                 <  2:10461235/150‑1 (MQ=255)
                                  aGTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGTCGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCGTCTGCTCCTGGTAAGCCTCGTGGGCCTCGCGCTCGTTTTCGGCGGCACGCTCGCGTGCCGCTTCCGCCAGGCGGGTg                                                                                                                >  1:10943157/1‑150 (MQ=255)
                                   gTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTgg                                                                                                               >  2:3256815/1‑150 (MQ=255)
                                   gTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTgg                                                                                                               >  2:57874/1‑150 (MQ=255)
                                    tAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGgc                                                                                                              <  1:5310009/150‑1 (MQ=255)
                                    tAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGgc                                                                                                              >  2:8282637/1‑150 (MQ=255)
                                     aGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGgcg                                                                                                             >  1:6066256/1‑150 (MQ=255)
                                     aGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGgcg                                                                                                             >  2:1662201/1‑150 (MQ=255)
                                         tttCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGa                                                                                                         >  1:5078159/1‑150 (MQ=255)
                                         tttCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGa                                                                                                         >  2:4039864/1‑150 (MQ=255)
                                           tCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAgg                                                                                                       <  1:9269706/150‑1 (MQ=255)
                                            ccTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGt                                                                                                      >  1:6930914/1‑150 (MQ=255)
                                             cTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTCAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGGGCCGCTTCCACCAGGCGGGTGGCGCTGAGGTa                                                                                                     >  1:10412549/1‑150 (MQ=255)
                                             cTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTa                                                                                                     >  2:3737461/1‑150 (MQ=255)
                                             cTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTa                                                                                                     <  1:2724426/150‑1 (MQ=255)
                                              tGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAg                                                                                                    >  1:8651849/1‑150 (MQ=255)
                                               ggATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGt                                                                                                   >  2:5051222/1‑150 (MQ=255)
                                                  tCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCt                                                                                                >  2:7418546/1‑150 (MQ=255)
                                                    aGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTgg                                                                                              >  2:671669/1‑150 (MQ=255)
                                                     gCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGt                                                                                             >  2:1498127/1‑150 (MQ=255)
                                                         ggCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTc                                                                                         >  1:4409275/1‑150 (MQ=255)
                                                         ggCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTc                                                                                         >  1:835870/1‑150 (MQ=255)
                                                          gCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTcg                                                                                        >  2:1904340/1‑150 (MQ=255)
                                                          gCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTcg                                                                                        <  1:9401176/150‑1 (MQ=255)
                                                              ggCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCggg                                                                                    <  1:3999666/150‑1 (MQ=255)
                                                              ggCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCggg                                                                                    >  2:8432506/1‑150 (MQ=255)
                                                                cgcAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCGGGTAGTCGCGGgcg                                                                                  >  2:9371550/1‑150 (MQ=255)
                                                                  cAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGgcgcg                                                                                >  2:10675587/1‑150 (MQ=255)
                                                                    gACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGGGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCa                                                                              >  1:253710/1‑150 (MQ=255)
                                                                         gcCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCCGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAgg                                                                         <  2:6940834/150‑1 (MQ=255)
                                                                           ctcctcCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCAGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGc                                                                       >  2:10334861/1‑150 (MQ=255)
                                                                              ctccAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGCTTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTg                                                                    >  1:10768500/1‑150 (MQ=255)
                                                                                ccAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGcc                                                                  >  1:6150213/1‑150 (MQ=255)
                                                                                ccAGTCCGGCCTCCCAGCCCAGGCGCTGCTGCTCCTGGTAAACCTCGGGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCGGAGGTCGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGcc                                                                  >  2:9174956/1‑150 (MQ=255)
                                                                                   gTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCgg                                                               <  2:10038858/150‑1 (MQ=255)
                                                                                   gTCCGGCCTCCCAGCCCAGCCGCGACTGCTCCGGGTAAACCTCGTGGGCCACGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGATGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCgg                                                               <  2:11003377/150‑1 (MQ=255)
                                                                                    tCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCggg                                                              <  2:9443199/150‑1 (MQ=255)
                                                                                    tCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCggg                                                              <  2:7240915/150‑1 (MQ=255)
                                                                                     ccGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTCGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCgggg                                                             >  1:3357000/1‑150 (MQ=255)
                                                                                     ccGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCgggg                                                             >  1:2134764/1‑150 (MQ=255)
                                                                                       ggCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGGTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGc                                                           >  1:1909257/1‑150 (MQ=255)
                                                                                        gCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGcc                                                          >  1:5677223/1‑150 (MQ=255)
                                                                                        gCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGcc                                                          <  1:5914199/150‑1 (MQ=255)
                                                                                          cTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAg                                                        >  2:8895228/1‑150 (MQ=255)
                                                                                          cTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAg                                                        >  2:7082315/1‑150 (MQ=255)
                                                                                               agcccagGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTa                                                   <  2:2556014/150‑1 (MQ=255)
                                                                                                   cagGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGGAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTAcgcg                                               >  1:4694204/1‑150 (MQ=255)
                                                                                                    agGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTCCGCGa                                              >  1:3849213/1‑150 (MQ=255)
                                                                                                     ggCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGAc                                             <  2:4136662/150‑1 (MQ=255)
                                                                                                      gcgcTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACt                                            <  2:8520102/150‑1 (MQ=255)
                                                                                                      gcgcTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACt                                            <  1:9004059/150‑1 (MQ=255)
                                                                                                         cTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGGACGCGACTGGc                                         >  1:5565443/1‑150 (MQ=255)
                                                                                                          ttCTGCTCCTGGTAACCCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCg                                        <  1:630393/150‑1 (MQ=255)
                                                                                                              gCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTa                                    <  1:4598330/150‑1 (MQ=255)
                                                                                                                tCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGt                                  >  1:6632862/1‑150 (MQ=255)
                                                                                                                 ccTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGtt                                 >  2:545203/1‑150 (MQ=255)
                                                                                                                  cTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTc                                <  2:4706497/150‑1 (MQ=255)
                                                                                                                        aaCCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTCAACaaa                          <  2:3592454/150‑1 (MQ=255)
                                                                                                                         aCCTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTCAACAAAt                         <  1:10545091/150‑1 (MQ=255)
                                                                                                                           cTCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTCACCACATgg                       <  2:1272654/150‑1 (MQ=255)
                                                                                                                            tCGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTCAACAAATGGa                      <  2:6577233/150‑1 (MQ=255)
                                                                                                                             cGTGGGCCTCGCGCTCTATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTCAACAAATGGaa                     <  1:10109307/150‑1 (MQ=255)
                                                                                                                             cGTGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTCAACAAATGGaa                     <  2:1343532/150‑1 (MQ=255)
                                                                                                                               tGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTCAACAAATGGAAGc                   >  2:6243156/1‑150 (MQ=255)
                                                                                                                               tGGGCCTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTCAACAAATGGAAGc                   >  2:9583887/1‑150 (MQ=255)
                                                                                                                                 ggCCTCGCGCTCGCTTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTCAACAAAGGGAAGCAt                 <  2:10577174/150‑1 (MQ=255)
                                                                                                                                   ccTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTCAACAAATGGAAGCATTc               <  1:7929508/150‑1 (MQ=255)
                                                                                                                                    cTCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTCAACAAATGGAAGCATTCa              >  1:7565912/1‑150 (MQ=255)
                                                                                                                                     tCGCGCTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTCAACAAATGGAAGCATTCAt             >  1:2389778/1‑150 (MQ=255)
                                                                                                                                       gcgcTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTCAACAAATGGAAGCATTCAtcg           <  1:9580994/150‑1 (MQ=255)
                                                                                                                                          cTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTCAACAAATGGAAGCATTCAtcgctc        <  2:4979529/150‑1 (MQ=255)
                                                                                                                                          cTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTCAACAAATGGAAGCATTCAtcgctc        >  1:2482913/1‑150 (MQ=255)
                                                                                                                                          cTCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGGTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTCAACAAATGGAAGCATTCAtcgctc        <  2:10799535/150‑1 (MQ=255)
                                                                                                                                           tCGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTCAACAAATGGAAGCATTCAtcgctcg       <  2:6142325/150‑1 (MQ=255)
                                                                                                                                            cGATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTCAACAAATGGAAGCATTCAtcgctcgc      <  2:9765628/150‑1 (MQ=255)
                                                                                                                                             gATTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTCAACAAATGGAAGCATTCATcgctcgct     >  1:7843296/1‑150 (MQ=255)
                                                                                                                                              aTTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTCGTCCTGGTTGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTCGTTCCACAAATGGACGCATTCAtcgctcgcgt    >  2:10253688/1‑148 (MQ=255)
                                                                                                                                              aTTTCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTCAACAAATGGAAGCATTCATCGCTCGCtt    >  1:1775592/1‑150 (MQ=255)
                                                                                                                                                ttCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTCAACAAATGGAAGCATTCATCGCTCGCTTGt  >  1:870451/1‑150 (MQ=255)
                                                                                                                                                ttCGGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTCAACAAATGGAAGCATTCATCGCTCGCTTGt  >  1:2854938/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GCGATCGACCTGCCGGTAGTAGCGATTGCAGCGCAGTAGCGTTTCCTGGATCAGCCCGGCCTGGCGCAGACGCGCCTCCTCCAGTCCGGCCTCCCAGCCCAGGCGCTTCTGCTCCTGGTAAACCTCGTGGGCCTCGCGCTCGATTTCCGCGGCACGCTCGCGTGCCGCTTCCACCAGGCGGTTGGCGCTGAGGTAGTCCTGGTAGTCGCGGGCGCGCAACAGGGCCTGCCCGGGGGCCAGCCGTACGCGACTGGCGTCTAGTTCAACAAATGGAAGCATTCATCGCTCGCTTGT  >  NC_008463/3769033‑3769326

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑