Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 3,879,553 A→G F52L (TTC→CTC)  PA14_RS17695 ← LysE family translocator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084633,879,5530AG99.5% 1603.5 / NA 417F52L (TTC→CTC) PA14_RS17695LysE family translocator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (224/191);  minor base T (0/2);  total (224/193)
Fisher's exact test for biased strand distribution p-value = 2.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 419 total aligned reads displayed.

CGTCGCCGCGCCGCAGCCAGGCGCGCAGCGCGCCGACGCCGAGCCAGGCCAGGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAAAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAACACCGCCGCCAGGGTCAGCAAGACAGACAATT  >  NC_008463/3879406‑3879699
                                                                                                                                                   |                                                                                                                                                  
cGTCGCCGCGCCGCAGCCAGGCGCGCAGCGCGCCGACGCCGAGCCAGGCCAGGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGagaa                                                                                                                                                  <  1:10413348/150‑1 (MQ=255)
cGTCGCCGCGCCGCAGCCAGGCGCGCAGCGCGCCGACGCCGAGCCAGGCCAGGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGagaa                                                                                                                                                  <  1:6633132/150‑1 (MQ=255)
 gTCGCCGCGCCGCAGCCAGGCGCGCAGCGCGCCGACGCCGAGCCAGGCCAGGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAAt                                                                                                                                                 <  2:9120150/150‑1 (MQ=255)
  tCGCCGCGCCGCAGCCAGGCGCGCAGCGCGCCGACGCCGAGCCAGGCCAGGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATc                                                                                                                                                <  2:7855740/150‑1 (MQ=255)
   cgccgcgccgCAGCCAGGCGCGCAGCGCGCCGACGCCGAGCCAGGCCAGGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATcc                                                                                                                                               <  2:1317496/150‑1 (MQ=255)
   cgccgcgccgCAGCCAGGCGCGCAGCGCGCCGACGCCGAGCCAGGCCAGGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATcc                                                                                                                                               <  2:6106712/150‑1 (MQ=255)
    gccgcgccgcAGCCAGGCGCGCAGCGCGCCGACGCCGAGCCAGGCCAGGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATccc                                                                                                                                              <  2:6001043/150‑1 (MQ=255)
    gccgcgccgcAGCCAGGCGCGCAGCGCGCCGACGCCGAGCCAGGCCAGGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATccc                                                                                                                                              >  1:1367064/1‑150 (MQ=255)
     ccgcgccgcAGCCAGGCGCGCAGCGCGCCGACGCCGAGCCAGGCCAGGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCg                                                                                                                                             <  2:4634927/150‑1 (MQ=255)
       gcgccgcAGCCAGGCGCGCAGCGCGCCGACGCCGAGCCAGGCCAGGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCa                                                                                                                                           >  2:6498150/1‑150 (MQ=255)
        cgccgcAGCCAGGCGCGCAGCGCGCCGACGCCGAGCCAGGCCAGGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAg                                                                                                                                          <  1:5708979/150‑1 (MQ=255)
          ccgcAGCCAGGCGCGCAGCGCGCCGACGCCGAGCCAGGCCAGGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGc                                                                                                                                        <  1:5819117/150‑1 (MQ=255)
             cAGCCAGGCGCGCAGCGCGCCGACGCCGAGCCAGGCCAGGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAg                                                                                                                                     >  2:8221126/1‑150 (MQ=255)
              aGCCAGGCGCGCAGCGCGCCGACGCCGAGCCAGGCCAGGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGc                                                                                                                                    <  2:9518335/150‑1 (MQ=255)
                ccAGGCGCGCAGCGCGCCGACGCCGAGCCAGGCCAGGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGccc                                                                                                                                  >  2:3657995/1‑150 (MQ=255)
                 cAGGCGCGCAGCGCGCCGACGCCGAGCCAGGCCAGGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGcccc                                                                                                                                 >  2:3235988/1‑150 (MQ=255)
                   ggCGCGCAGCGCGCCGACGCCGAGCCAGGCCAGGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAg                                                                                                                               >  1:9742709/1‑150 (MQ=255)
                    gcgcgcAGCGCGCCGACTCCTAGCAAGGCCTTCTACAGAGCGCTGAGCGCCTGATGGATGGCGAACAGCACAGGAGTACGGATGTTAAGCAGAGTCACGCCGTTCTGCTCCTGGTTGGTATGCAGGAGAAACCCGCAGGCCAGCCCCAgc                                                                                                                              <  2:2846482/150‑1 (MQ=255)
                    gcgcgcAGCGCGCCGACGCCGAGCCAGGCCAGGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAgc                                                                                                                              >  1:8667181/1‑150 (MQ=255)
                      gcgcAGCGCGCCGACGCCGAGCCAGGCCAGGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAgcgc                                                                                                                            >  2:6633834/1‑150 (MQ=255)
                       cgcAGCGCGCCGACGCCGAGCCAGGCCAGGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAgcgcg                                                                                                                           >  2:2162631/1‑150 (MQ=255)
                        gcAGCGCGCCGACGCCGAGCCAGGCCAGGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAgcgcgc                                                                                                                          <  2:2044968/150‑1 (MQ=255)
                           gcgcgcCGACGCCGAGCCAGGCCAGGTACAGCGCGCCGACCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCCGCACCAGGCTGGGATGCCGGAGAATACCGCAGGCCAGCCCCAGCGCGCTgg                                                                                                                       >  1:9132528/1‑150 (MQ=255)
                              cgccgacgccgaGCCAGGCCAGGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCa                                                                                                                    >  2:5640662/1‑150 (MQ=255)
                                ccgacgccgaGCCAGGCCAGGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAgg                                                                                                                  >  1:10937970/1‑150 (MQ=255)
                                 cgacgccgaGCCAGGCCAGGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAggc                                                                                                                 <  1:9051084/150‑1 (MQ=255)
                                  gacgccgaGCCAGGCCAGGTACAGCGCGCCGACCGCCTGCAGGATGTCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGAAACGCCGGTCAGCACCAGTGTGGTATGCAGTAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAggcc                                                                                                                >  2:3429967/1‑150 (MQ=255)
                                     gccgaGCCAGGCCAGGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAggccggc                                                                                                             >  2:2567948/1‑150 (MQ=255)
                                     gccgaGCCAGGCCAGGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAggccggc                                                                                                             <  1:7310949/150‑1 (MQ=255)
                                      ccgaGCCAGGCCAGGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAggccggcc                                                                                                            <  1:7220849/150‑1 (MQ=255)
                                         aGCCAGGCCAGGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCccgc                                                                                                         <  2:6774583/150‑1 (MQ=255)
                                             aggccaggTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCgccgcc                                                                                                     >  2:1676779/1‑150 (MQ=255)
                                                ccaggTACAGCGCGCCGAGCGCCTGCTGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGccccg                                                                                                  >  1:3609121/1‑149 (MQ=255)
                                                  aggTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTgg                                                                                                >  1:6381678/1‑150 (MQ=255)
                                                  aggTACAGCGCGCCGAGCGCCAGCAGGCTGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTgg                                                                                                <  2:7406401/150‑1 (MQ=255)
                                                     tACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGc                                                                                             >  1:3020833/1‑150 (MQ=255)
                                                      aCAGCGCGCCGAGCGCCTGCAGGATGGCGCACAGCACAGGCGTCCGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCa                                                                                            <  2:4236571/150‑1 (MQ=255)
                                                        aGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAgg                                                                                          >  1:6546204/1‑150 (MQ=255)
                                                          cgcgcCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGa                                                                                        <  1:6078580/150‑1 (MQ=255)
                                                          cgcgcCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGa                                                                                        <  1:9319951/150‑1 (MQ=255)
                                                             gcCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGt                                                                                     >  1:5185409/1‑150 (MQ=255)
                                                              ccGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTg                                                                                    >  1:3200147/1‑150 (MQ=255)
                                                              ccGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTg                                                                                    <  2:10200258/150‑1 (MQ=255)
                                                              cagAGGGCCTGCTGGATGGCGTTCAGCACATGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGATAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTg                                                                                    <  2:8744937/148‑1 (MQ=255)
                                                                   cgcCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGcac                                                                               >  1:10810499/1‑150 (MQ=255)
                                                                    gcCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGcacc                                                                              >  1:9382021/1‑150 (MQ=255)
                                                                     ccTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGcacca                                                                             >  1:248379/1‑150 (MQ=255)
                                                                      cTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGcaccac                                                                            <  2:8066008/150‑1 (MQ=255)
                                                                      cTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGcaccac                                                                            <  1:6478868/150‑1 (MQ=255)
                                                                       tGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCacctcc                                                                           >  1:5765331/1‑150 (MQ=255)
                                                                       tGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCaccacc                                                                           <  2:8392246/150‑1 (MQ=255)
                                                                        gCATGAGGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCAccacca                                                                          <  1:4250954/150‑1 (MQ=255)
                                                                          aGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAgg                                                                        >  1:1532459/1‑150 (MQ=255)
                                                                          aGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAgg                                                                        <  2:7958948/150‑1 (MQ=255)
                                                                          aGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAgg                                                                        >  1:7837465/1‑150 (MQ=255)
                                                                           ggATGGCGAACAGCACCGGCTTACGGCTGATCAGCAGCCACACGCCGTACAGCACCTGGGTGTTATGCAGTATAATCCCGAAGGCCCGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCTGGAACTTGCGCACCACCAggg                                                                       <  2:1594504/150‑1 (MQ=255)
                                                                           ggATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAggg                                                                       >  1:1991/1‑150 (MQ=255)
                                                                            gATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGc                                                                      >  2:2312690/1‑150 (MQ=255)
                                                                            gATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGc                                                                      <  1:3285922/150‑1 (MQ=255)
                                                                               ggCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGAc                                                                   >  2:5925129/1‑150 (MQ=255)
                                                                                gCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACa                                                                  >  2:1010213/1‑150 (MQ=255)
                                                                                      aGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCCGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGa                                                            >  1:10266127/1‑150 (MQ=255)
                                                                                        cacaGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGAcc                                                          >  1:747874/1‑150 (MQ=255)
                                                                                        cacaGGCGGACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGAcc                                                          <  1:9974078/150‑1 (MQ=255)
                                                                                         acaGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCg                                                         <  1:3688887/150‑1 (MQ=255)
                                                                                           aGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAGTCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCggg                                                       >  2:7770441/1‑150 (MQ=255)
                                                                                            ggCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCgggg                                                      <  1:591976/150‑1 (MQ=255)
                                                                                               gTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTg                                                   <  2:9673982/150‑1 (MQ=255)
                                                                                                 aCGGCAGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGAc                                                 <  1:2853536/150‑1 (MQ=255)
                                                                                                    gCTTAGCAGCAGCGACACTCCGGTCAGCACCAGGCTGGTATGCACGAGAAACCCGCAGGCCAGCCCCAGCGCGCTGGCCTGGCCGGCCCGCCGCCCCTGGTGCAGGGTCGTGCGCACCACCAGGGCGACATCGTGTCCGGGGCTGACCAg                                              <  2:10430889/150‑1 (MQ=255)
                                                                                                    gCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAg                                              <  2:9250280/150‑1 (MQ=255)
                                                                                                      tGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCCGCCCCCGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAggg                                            >  1:7077366/1‑150 (MQ=255)
                                                                                                         gcagcagcGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGa                                         >  2:11047619/1‑150 (MQ=255)
                                                                                                          cagcagcGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGag                                        >  2:7008615/1‑149 (MQ=255)
                                                                                                          cagcagcGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGAc                                        >  2:407219/1‑150 (MQ=255)
                                                                                                           agcagcGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGAGCAGGGCGTCg                                       >  1:10131207/1‑150 (MQ=255)
                                                                                                           agcagcGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACg                                       <  2:4990621/150‑1 (MQ=255)
                                                                                                           agcagcGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACg                                       >  1:7413992/1‑150 (MQ=255)
                                                                                                            gcagcGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACgg                                      >  2:7509741/1‑150 (MQ=255)
                                                                                                                 gACACGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAac                                 >  2:7202464/1‑150 (MQ=255)
                                                                                                                   cacGCCGGTCAGCACCCGGGTGGTATGCAGGAGCATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCCGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAacac                               <  2:5720341/150‑1 (MQ=255)
                                                                                                                    acGCCGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAACAcc                              <  1:8126215/150‑1 (MQ=255)
                                                                                                                        cGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAACAccgccg                          <  2:5290150/150‑1 (MQ=255)
                                                                                                                        cGGTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAACAccgccg                          <  1:10574706/150‑1 (MQ=255)
                                                                                                                         ggTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAACACcgccgc                         >  1:827439/1‑150 (MQ=255)
                                                                                                                          gTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAACACCgccgcc                        >  2:309635/1‑150 (MQ=255)
                                                                                                                          gTCAGCACCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCGGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAACACCgccgcc                        <  2:9570788/150‑1 (MQ=255)
                                                                                                                                aCCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAACACCGCCGCCAGGGTc                  <  2:4364990/150‑1 (MQ=255)
                                                                                                                                aCCAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAACACCGCCGCCAGGGTc                  >  2:1465981/1‑150 (MQ=255)
                                                                                                                                  cAGGGTGGTATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAACACCGCCGCCAGGGTCAg                <  1:9568935/150‑1 (MQ=255)
                                                                                                                                    gggTGGTCTGCAGGCGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAACACCGCCGCCCGGGTCTGct              >  2:7625403/1‑149 (MQ=255)
                                                                                                                                     ggtggtATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAACACCGCCGCCAGGGTCAGCaa             <  1:4583684/150‑1 (MQ=255)
                                                                                                                                       tggtATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAACACCGCCGCCAGGGTCAGCAaga           >  2:10820413/1‑150 (MQ=255)
                                                                                                                                        ggtATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAACACCGCCGCCAGGGTCAGCAagac          <  2:1061580/150‑1 (MQ=255)
                                                                                                                                          tATGCAGGAGAATCCCTCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGTTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAACACCGCCGCCAGGGTCAGCAagacag        <  1:1125824/150‑1 (MQ=255)
                                                                                                                                          tATGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAACACCGCCGCCAGGGTCAGCAagacag        <  1:7679827/150‑1 (MQ=255)
                                                                                                                                            tGCAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAACACCGCCGCCAGGGTCAGCAagacagac      <  1:5511397/150‑1 (MQ=255)
                                                                                                                                              cAGGAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAACACCGCCGCCAGGGTCAGCAAGACAGACaa    <  1:6884339/150‑1 (MQ=255)
                                                                                                                                                ggAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAACACCGCCGCCAGGGTCAGCAAGACAGACAAtt  <  1:4705508/150‑1 (MQ=255)
                                                                                                                                                ggAGAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAACACCGCCGCCAGGGTCAGCAAGACAGACAAtt  >  1:374320/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CGTCGCCGCGCCGCAGCCAGGCGCGCAGCGCGCCGACGCCGAGCCAGGCCAGGTACAGCGCGCCGAGCGCCTGCAGGATGGCGAACAGCACAGGCGTACGGCTGAGCAGCAGCGACACGCCGGTCAGCACCAGGGTGGTATGCAGGAAAATCCCGCAGGCCAGCCCCAGCGCGCTGGCCAGGCCGGCCCGCCGCCCCTGGTGCAGGGACGTGCGCACCACCAGGGCGACATCGGGACCGGGGCTGACCAGGGCGACGGCGAACACCGCCGCCAGGGTCAGCAAGACAGACAATT  >  NC_008463/3879406‑3879699

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑