Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 4,565,093 C→G G290R (GGC→CGC)  PA14_RS20885 ← anthranilate synthase component 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084634,565,0930CG99.8% 2102.6 / NA 545G290R (GGC→CGC) PA14_RS20885anthranilate synthase component 1
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base G (263/281);  minor base T (0/1);  total (263/282)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 545 total aligned reads displayed.

CATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCCCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCAAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCCCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGCCCTCACGTGGGCCTGCAGGCGCTCCACCTGGCGAGCAAAGCTCGCATCGTCCAGGTCG  >  NC_008463/4564946‑4565238
                                                                                                                                                   |                                                                                                                                                 
cATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCa                                                                                                                                                 >  1:8269602/1‑150 (MQ=255)
cATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCa                                                                                                                                                 <  2:89365/150‑1 (MQ=255)
 aTCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCAGCGAGGACGAAGCGGTACGGGCTGGGGTTGCGCAg                                                                                                                                                >  2:3070712/1‑150 (MQ=255)
  tCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAgg                                                                                                                                               >  1:9184933/1‑150 (MQ=255)
  tCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAgg                                                                                                                                               >  1:909482/1‑150 (MQ=255)
      gTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGcaca                                                                                                                                           >  1:2967820/1‑150 (MQ=255)
            gcgGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGc                                                                                                                                     >  2:9633216/1‑150 (MQ=255)
              gggCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGa                                                                                                                                   <  1:1574948/150‑1 (MQ=255)
               ggCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGAt                                                                                                                                  >  2:4677702/1‑150 (MQ=255)
                gCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATa                                                                                                                                 >  1:1962952/1‑150 (MQ=255)
                  gAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAgg                                                                                                                               <  1:8447562/150‑1 (MQ=255)
                  gAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAgg                                                                                                                               >  1:6996637/1‑150 (MQ=255)
                   aGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGc                                                                                                                              <  2:7255990/150‑1 (MQ=255)
                    gTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGACGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGcc                                                                                                                             >  2:1721600/1‑150 (MQ=255)
                    gTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGcc                                                                                                                             <  1:5365739/150‑1 (MQ=255)
                    gTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGcc                                                                                                                             <  2:8102061/150‑1 (MQ=255)
                    gTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGcc                                                                                                                             <  2:3761283/150‑1 (MQ=255)
                         ggCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCa                                                                                                                        <  2:940058/150‑1 (MQ=255)
                          gCAATGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAg                                                                                                                       >  2:916786/1‑150 (MQ=255)
                              tGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGt                                                                                                                   <  1:4499614/150‑1 (MQ=255)
                              tGGGGTAGAGTTCCACCTCGCGACTCTCCGCGTCGAACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTCCGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGt                                                                                                                   >  1:7117691/1‑150 (MQ=255)
                              tGGGGTAGAGTTCCACCTCGCGACTCGCCGCGTCGTACTTCACTGCCGCCTCCGGCGAAGCGCCGAACAGGCAGCAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGt                                                                                                                   <  2:2913292/150‑1 (MQ=255)
                                gggTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTcc                                                                                                                 >  2:874739/1‑150 (MQ=255)
                                 ggTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACCGGCAGAAGTCCCCCGCCTCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACCACTGGCGATAGGCCCGCCAGGGGTCcg                                                                                                                >  2:2842769/1‑150 (MQ=255)
                                 ggTAGAGTTCCACCTCGCGACTCTCCGCGTCGGACTTCACTGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAATAAGCGGTACGGGCTGGGGTTGCGCAGGCACACCTGGCGATAGGCCCGCCAGGGGTCcg                                                                                                                <  2:6868058/150‑1 (MQ=255)
                                  gTAGAGTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCcgc                                                                                                               >  2:8494521/1‑150 (MQ=255)
                                    agagTTCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCgcgc                                                                                                             >  2:4946958/1‑150 (MQ=255)
                                        ttCCACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACgg                                                                                                         >  2:3198021/1‑150 (MQ=255)
                                          ccACCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCa                                                                                                       <  2:5555579/150‑1 (MQ=255)
                                            aCCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATg                                                                                                     >  1:9570245/1‑150 (MQ=255)
                                            aCCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATg                                                                                                     >  1:1414638/1‑150 (MQ=255)
                                            aCCTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATg                                                                                                     >  2:1341843/1‑150 (MQ=255)
                                             ccTCGCGACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGc                                                                                                    <  1:10698392/150‑1 (MQ=255)
                                                 gcgACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGaa                                                                                                >  2:6029768/1‑150 (MQ=255)
                                                   gACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGc                                                                                              <  1:5596846/150‑1 (MQ=255)
                                                   gACTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGc                                                                                              <  1:9815358/150‑1 (MQ=255)
                                                    aCTCTCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGACGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCt                                                                                             >  2:9292845/1‑150 (MQ=255)
                                                     ctctCCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTg                                                                                            <  1:3881630/150‑1 (MQ=255)
                                                      tctcCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTgc                                                                                           <  1:5002012/150‑1 (MQ=255)
                                                      tctcCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTgc                                                                                           <  2:3436185/150‑1 (MQ=255)
                                                       ctcCGCGTCGTACTTCACTGCCGACTCCGGCGAAGCGCCGAACAGGCAGCAGTCCCCCGCATCGAGGAATAAGCGGTACGGGCTGGGGTTGCTCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTgcg                                                                                          <  2:5136162/150‑1 (MQ=255)
                                                       ctcCGCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTgcg                                                                                          >  2:8636126/1‑150 (MQ=255)
                                                          cgcgtcgtACTTCAAGGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGa                                                                                       <  1:10096720/148‑1 (MQ=255)
                                                              tcgtACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGt                                                                                   <  2:10096136/150‑1 (MQ=255)
                                                              tcgtACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGt                                                                                   >  2:1157610/1‑150 (MQ=255)
                                                                gtACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTAc                                                                                 >  2:4442329/1‑150 (MQ=255)
                                                                 tACTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACg                                                                                >  1:7852472/1‑150 (MQ=255)
                                                                  aCTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCTGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGa                                                                               <  1:8535660/150‑1 (MQ=255)
                                                                  aCTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGa                                                                               >  2:7982122/1‑150 (MQ=255)
                                                                  aCTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGa                                                                               <  1:10975386/150‑1 (MQ=255)
                                                                   cTTCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGAt                                                                              >  1:6216319/1‑150 (MQ=255)
                                                                    ttCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATc                                                                             <  1:4238367/150‑1 (MQ=255)
                                                                     tCAATGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCt                                                                            >  2:9840799/1‑150 (MQ=255)
                                                                       aaTGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTgg                                                                          >  1:10824175/1‑150 (MQ=255)
                                                                         tGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGaa                                                                        <  1:6042833/150‑1 (MQ=255)
                                                                         tGCCGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGaa                                                                        >  1:1041510/1‑150 (MQ=255)
                                                                            cGACTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAacac                                                                     <  2:4189841/150‑1 (MQ=255)
                                                                              aCTCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGt                                                                   >  1:7985878/1‑150 (MQ=255)
                                                                                tCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCg                                                                 >  1:2197046/1‑150 (MQ=255)
                                                                                tCCGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCg                                                                 >  1:6817369/1‑150 (MQ=255)
                                                                                  cGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGcc                                                               <  1:3721252/150‑1 (MQ=255)
                                                                                  cGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGcc                                                               <  2:1463701/150‑1 (MQ=255)
                                                                                  cGGCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGcc                                                               <  1:6554123/150‑1 (MQ=255)
                                                                                   ggCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGCTAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTCCGATCTGGAACACGTCGCCg                                                              >  2:10596371/1‑150 (MQ=255)
                                                                                    gCGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCgg                                                             <  2:2059712/150‑1 (MQ=255)
                                                                                     cGAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGc                                                            >  2:10334689/1‑150 (MQ=255)
                                                                                      gAAGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGcc                                                           >  1:7521918/1‑150 (MQ=255)
                                                                                       aaGCGCCGAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGccc                                                          >  1:3531996/1‑150 (MQ=255)
                                                                                             cgaaCAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGCCCTCACGt                                                    >  1:330807/3‑150 (MQ=255)
                                                                                                  aGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGCCCTCACGTGGGcc                                               <  1:9425657/150‑1 (MQ=255)
                                                                                                  aGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGCCCTCACGTGGGcc                                               >  2:5319436/1‑150 (MQ=255)
                                                                                                     cAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGCCCTCACGTGGGCCTGc                                            <  2:6698558/150‑1 (MQ=255)
                                                                                                     cAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGCCCTCACGTGGGCCTGc                                            <  2:2690228/150‑1 (MQ=255)
                                                                                                     cAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGCCCTCACGTGGGCCTGc                                            <  2:6317870/150‑1 (MQ=255)
                                                                                                     cAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGCCCTCACGTGGGCCTGc                                            <  2:7603184/150‑1 (MQ=255)
                                                                                                      aGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGCTAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGCCCTCACGTGGGCCTGCa                                           <  1:2764353/150‑1 (MQ=255)
                                                                                                           tCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGCCCTCACGTGGGCCTGCAGgcgc                                      >  2:6216742/1‑150 (MQ=255)
                                                                                                            cccccGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGCCCTCACGTGGGCCTGCAGGCGCt                                     >  1:2109946/1‑150 (MQ=255)
                                                                                                              cccGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGCCCTCACGTGGGCCTGCAGGCGCTcc                                   >  1:8751805/1‑150 (MQ=255)
                                                                                                               ccGCATCGAGGACGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGCCCTCACGTGGGCCTGCAGGCGCTCCa                                  >  2:7404289/1‑150 (MQ=255)
                                                                                                               ccGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGCCCTCACGTGGGCCTGCAGGCGCTCCa                                  >  1:2362951/1‑150 (MQ=255)
                                                                                                                 gCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGCCCTCACGTGGGCCTGCAGGCGCTCCAcc                                <  2:2202282/150‑1 (MQ=255)
                                                                                                                    tCGAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGCCCTCACGTGGGCCTGCAGGCGCTCCACCTgg                             >  1:1846334/1‑150 (MQ=255)
                                                                                                                      gAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGCCCTCACGTGGGCCTGCAGGCGCTCCACCTGGCg                           >  2:7887035/1‑150 (MQ=255)
                                                                                                                      gAGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTAGCCGGCCCTCACGTGGGCCTGCAGGCGCTCCACCTGGCg                           <  2:2333506/150‑1 (MQ=255)
                                                                                                                       aGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGCCCTCACGTGGGCCTGCAGGCGCTCCACCTGGCGa                          <  1:10020293/150‑1 (MQ=255)
                                                                                                                       aGGAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGCCCTCACGTGGGCCTGCAGGCGCTCCACCTGGCGa                          <  1:241975/150‑1 (MQ=255)
                                                                                                                        ggAAGAAGCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGCCCTCACGTGGGCCTGCAGGCGCTCCACCTGGCGAg                         >  2:4608282/1‑150 (MQ=255)
                                                                                                                          aagaagCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGACCACGTCGCCGGCCCTCCCGTGGGCCTGCAGGCGCTCCACCTGGCGAGCa                       <  2:7738437/150‑1 (MQ=255)
                                                                                                                           agaagCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGCCCTCACGTGGGCCTGCAGGCGCTCCACCTGGCGAGCaa                      <  1:10535499/150‑1 (MQ=255)
                                                                                                                            gaagCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGCCCTCACGTGGGCCTGCAGGCGCTCCACCTGGCGAGCaaa                     >  2:5898687/1‑150 (MQ=255)
                                                                                                                              agCGGTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGCCCTCACGTGGGCCTGCAGGCGCTCCACCTGGCGAGCAAAGc                   <  1:1241642/150‑1 (MQ=255)
                                                                                                                                 ggTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGCCCTCACGTGGGCCTGCAGGCGCTCCACCTGGCGAGCAAAGCTCg                >  2:4081020/1‑150 (MQ=255)
                                                                                                                                  gTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGCCCTCACGTGGGCCTGCTGGCGCTCCACCTGGCGAGCAAAGCTCGc               >  2:1821352/1‑150 (MQ=255)
                                                                                                                                  gTACGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGCCCTCACGTGGGCCTGCAGGCGCTCCACCTGGCGAGCAAAGCTCGc               <  2:8315216/150‑1 (MQ=255)
                                                                                                                                    aCGGGCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGCCCTCACGTGGGCCTGCAGGCGCTCCACCTGGCGAGCAAAGCTCGCAt             <  1:3741790/150‑1 (MQ=255)
                                                                                                                                       ggCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGCCCTCACGTGGGCCTGCAGGCGCTCCACCTGGCGAGCAAAGCTCGCATCGt          >  1:8096702/1‑150 (MQ=255)
                                                                                                                                       ggCTGGGGTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATATGGAACACGTCGCCGGCCCTCACGTGGGCCTGCAGGCGCTCCACCTGGCGAGCAAAGCTCGCATCGt          <  1:3161648/150‑1 (MQ=255)
                                                                                                                                            gggTTGCGCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGCCCTCACGTGGGCCTGCAGGCGCTCCACCTGGCGAGCAAAGCTCGCATCGTCCAgg     <  2:2518988/150‑1 (MQ=255)
                                                                                                                                              cttGCGCAGGCACAACTGGCGATCGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGCCCTCACGTGGGCCTGCAGGCGCTCCACCTGGCGAGCAAAGCTCGCATCGTCCAGGTc   <  2:6540593/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
CATCGCGTCCGCGCGGGCGAGTGCCGGCAATGGGGTAGAGTTCCACCTCGCGACTCTCCCCGTCGTACTTCAATGCCGACTCCGGCGAAGCGCCAAACAGGCAGAAGTCCCCCGCATCGAGGAAGAAGCGGTACGGGCTGGGGTTGCCCAGGCACAACTGGCGATAGGCCCGCCAGGGGTCCGCGCACGGCATGCTGAAGCTGCGCGACGGTACGATCTGGAACACGTCGCCGGCCCTCACGTGGGCCTGCAGGCGCTCCACCTGGCGAGCAAAGCTCGCATCGTCCAGGTCG  >  NC_008463/4564946‑4565238

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑