Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 4,707,787 G→C A208A (GCG→GCC)  PA14_RS21625 → zinc‑binding alcohol dehydrogenase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084634,707,7870GC100.0% 2041.4 / NA 525A208A (GCG→GCC) PA14_RS21625zinc‑binding alcohol dehydrogenase family protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (246/279);  total (246/279)

Only 100 of 556 total aligned reads displayed.

GGCCAAGGCCCTGGGGGCGGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCGGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGAACCTGTACCGTCGCGGTGGCTCGGT  >  NC_008463/4707640‑4707933
                                                                                                                                                   |                                                                                                                                                  
ggcCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGa                                                                                                                                                  <  2:4060703/150‑1 (MQ=255)
ggcCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGa                                                                                                                                                  <  1:10990476/150‑1 (MQ=255)
  ccAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCNGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAgg                                                                                                                                                <  1:5786170/150‑1 (MQ=255)
  ccAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAgg                                                                                                                                                <  1:7931749/150‑1 (MQ=255)
  ccAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAgg                                                                                                                                                >  1:1266004/1‑150 (MQ=255)
  ccAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAgg                                                                                                                                                <  2:8191036/150‑1 (MQ=255)
   cAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGt                                                                                                                                               <  2:7541429/150‑1 (MQ=255)
    aaGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTg                                                                                                                                              <  1:2790208/150‑1 (MQ=255)
     aGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGa                                                                                                                                             >  2:6082362/1‑150 (MQ=255)
     aGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGa                                                                                                                                             >  2:1722773/1‑150 (MQ=255)
         ccTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCtt                                                                                                                                         <  1:10233124/150‑1 (MQ=255)
           tGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCg                                                                                                                                       >  2:3639805/1‑150 (MQ=255)
            gggggCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGa                                                                                                                                      <  2:7568756/150‑1 (MQ=255)
             ggggCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGAt                                                                                                                                     <  2:3880429/150‑1 (MQ=255)
              gggCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATa                                                                                                                                    <  1:5984002/150‑1 (MQ=255)
               ggCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATAc                                                                                                                                   <  2:7005921/150‑1 (MQ=255)
                gccgAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTTATCTTCGATACg                                                                                                                                  <  2:8911939/147‑1 (MQ=255)
                 ccgAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGa                                                                                                                                 <  2:4603404/148‑1 (MQ=255)
                  cgAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGACGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGAc                                                                                                                                >  1:2128285/2‑150 (MQ=255)
                  cgAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGAc                                                                                                                                <  2:288944/149‑1 (MQ=255)
                   gAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGAcc                                                                                                                               <  1:10617204/150‑1 (MQ=255)
                   gAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGAcc                                                                                                                               <  1:6635335/150‑1 (MQ=255)
                   gAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGAGACGAcc                                                                                                                               >  1:255864/1‑150 (MQ=255)
                     aGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCgg                                                                                                                             <  1:1917484/150‑1 (MQ=255)
                      gCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGt                                                                                                                            <  2:428965/150‑1 (MQ=255)
                       cGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGtt                                                                                                                           >  2:807390/1‑150 (MQ=255)
                       cGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGtt                                                                                                                           >  2:5222880/1‑150 (MQ=255)
                       cGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGtt                                                                                                                           <  2:2380356/150‑1 (MQ=255)
                         aTCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTc                                                                                                                         >  1:1794067/1‑150 (MQ=255)
                         aTCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTc                                                                                                                         >  1:833325/1‑150 (MQ=255)
                          tCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCt                                                                                                                        <  1:1395971/150‑1 (MQ=255)
                          tCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCt                                                                                                                        <  2:27910/150‑1 (MQ=255)
                            ggCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTgg                                                                                                                      >  2:9558274/1‑150 (MQ=255)
                              cgcgGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCt                                                                                                                    <  2:3541186/150‑1 (MQ=255)
                               gcgGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTc                                                                                                                   <  1:4307079/150‑1 (MQ=255)
                                 ggTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGa                                                                                                                 <  1:3559086/150‑1 (MQ=255)
                                   tGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAgg                                                                                                               <  1:4865499/150‑1 (MQ=255)
                                   tGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAgg                                                                                                               <  2:4726623/150‑1 (MQ=255)
                                       gcgcGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAggcggc                                                                                                           <  2:3055360/150‑1 (MQ=255)
                                         gcgAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGcggcgg                                                                                                         <  2:5516823/150‑1 (MQ=255)
                                           gaggagCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGACCTGGCGGCACCGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGAGACGACCGGTTTCTGGCTCGTGGcggcgggc                                                                                                       >  2:10649201/1‑148 (MQ=255)
                                            aggagCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTcc                                                                                                      <  2:257034/150‑1 (MQ=255)
                                             ggagCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTccc                                                                                                     >  1:8871350/1‑150 (MQ=255)
                                                 cAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGgcg                                                                                                 >  2:8269539/1‑150 (MQ=255)
                                                      ccAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGAGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGGTTCTGGCTCGAGGCGGCGGTCCCGTCGCTgtc                                                                                            >  2:5837743/1‑148 (MQ=255)
                                                         ggCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGgccgc                                                                                         <  1:9311296/150‑1 (MQ=255)
                                                           cgcTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCt                                                                                       <  1:3109871/150‑1 (MQ=255)
                                                           cgcTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCt                                                                                       <  1:8637088/150‑1 (MQ=255)
                                                           cgcTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCt                                                                                       <  2:1241030/150‑1 (MQ=255)
                                                            gcTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCtt                                                                                      <  1:7262380/150‑1 (MQ=255)
                                                                   gcgcAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGGGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGc                                                                               >  2:1927747/1‑150 (MQ=255)
                                                                     gcAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCt                                                                             >  2:6512082/1‑150 (MQ=255)
                                                                           cATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGat                                                                       <  1:6899640/150‑1 (MQ=255)
                                                                              cgccgcGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGatcat                                                                    <  2:8607934/150‑1 (MQ=255)
                                                                               gccgcGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGatcatc                                                                   <  1:8103854/150‑1 (MQ=255)
                                                                                ccgaGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATcg                                                                  >  2:9316732/1‑150 (MQ=255)
                                                                                  gcgcTGCTGCTGGGCGAGCCGGCCGACCTGGCGGCACAGGTGGAAGGGCTCTTCCCCGGCGGGGCCGAGGTGATCTGCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATcgcc                                                                >  2:10943724/1‑150 (MQ=255)
                                                                                  gcgcTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATcgcc                                                                >  2:5890050/1‑150 (MQ=255)
                                                                                      tgctgctgGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCAc                                                            <  2:9735853/150‑1 (MQ=255)
                                                                                       gctgcttGGCGAGCCGGCCCAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATAAGAACGGTTTCAGGCTCGAGGCGGCGGACCAGGCTCTGGCCGCCAGCGGACGCCTGGCGATCATCGCCGCaac                                                           >  2:2174528/1‑148 (MQ=255)
                                                                                        ctgctgGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCa                                                          <  2:8869115/150‑1 (MQ=255)
                                                                                         tgctgGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAg                                                         <  1:5548145/150‑1 (MQ=255)
                                                                                         tgctgGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAg                                                         >  2:10058055/1‑150 (MQ=255)
                                                                                           ctgGGCGAGCCGGGCGTACTGGCGGCACAGGTGGACGGGATCTTCCCCGGCGGGGGCGAGGTGATCTTCGATACGACCGGGTTCTGGCTCGAGGCGGCGGTCCGGGCGCTGGCCGCCTTCGGTCGCCTGGCGGTCATCGCCGCTCCGGcc                                                       >  2:624449/1‑150 (MQ=255)
                                                                                            tgGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCg                                                      >  1:10004592/1‑150 (MQ=255)
                                                                                             gggCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGa                                                     >  1:1486762/1‑150 (MQ=255)
                                                                                                 gAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGc                                                 <  1:9590015/150‑1 (MQ=255)
                                                                                                 gAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCAGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGc                                                 >  1:457034/1‑150 (MQ=255)
                                                                                                      ggccgAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCAtgt                                            <  2:7327084/150‑1 (MQ=255)
                                                                                                        ncgAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTgc                                          >  1:8517120/2‑150 (MQ=255)
                                                                                                        ccgAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTgc                                          >  1:9824978/1‑150 (MQ=255)
                                                                                                          gAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTgcgc                                        >  1:7055754/1‑150 (MQ=255)
                                                                                                            aCTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCt                                      >  1:3841810/1‑150 (MQ=255)
                                                                                                            aCTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCt                                      >  2:5699145/1‑150 (MQ=255)
                                                                                                              tGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGc                                    >  1:9206173/1‑150 (MQ=255)
                                                                                                              tGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGc                                    <  2:7227037/150‑1 (MQ=255)
                                                                                                                gcggcACAGGTGGAAGGGATCTTCCCCGGCGGTGCCGAGGTGATCTTCGATACGTCCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCg                                  <  2:1436165/150‑1 (MQ=255)
                                                                                                                 cggcACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCgg                                 <  1:7398769/150‑1 (MQ=255)
                                                                                                                   gcACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCg                               <  1:229078/150‑1 (MQ=255)
                                                                                                                     acaGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGtt                             >  2:501726/1‑150 (MQ=255)
                                                                                                                        ggTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGaa                          <  1:4756630/150‑1 (MQ=255)
                                                                                                                        ggTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGaa                          >  2:3185232/1‑150 (MQ=255)
                                                                                                                        ggTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCGGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGaa                          <  2:7110115/150‑1 (MQ=255)
                                                                                                                            gAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGAAcccg                      >  2:316566/1‑148 (MQ=255)
                                                                                                                            gAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGAACCTg                      >  2:8432/1‑150 (MQ=255)
                                                                                                                             aaGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGAACCTGt                     >  2:10587216/1‑150 (MQ=255)
                                                                                                                               gggATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGAACCTGTAc                   >  1:6387267/1‑150 (MQ=255)
                                                                                                                               gggATCGACCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGAACCTGTAc                   <  2:10341803/150‑1 (MQ=255)
                                                                                                                                  aTCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGAACCTGTACCGt                <  1:4701378/150‑1 (MQ=255)
                                                                                                                                   tCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGAACCTGTACCGTc               <  2:5414806/150‑1 (MQ=255)
                                                                                                                                    cTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGAACCTGTACCGTcg              >  2:10755648/1‑150 (MQ=255)
                                                                                                                                     ttCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGAACCTGTACCGTcgc             >  1:4364334/1‑150 (MQ=255)
                                                                                                                                       ccccGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGAACCTGTACCGTCGCgg           <  1:9492277/150‑1 (MQ=255)
                                                                                                                                        cccGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGAACCTGTACCGTCGCGGt          <  1:10526851/150‑1 (MQ=255)
                                                                                                                                         ccGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGAACCTGTACCGTCGCGGTg         >  1:4089587/1‑150 (MQ=255)
                                                                                                                                           ggcggggccgAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGAACCTGTACCGTCGCGGTGGc       >  2:1123259/1‑150 (MQ=255)
                                                                                                                                            gcggggccgAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGAACCTGTACCGTCGCGGTGGCt      <  1:9711671/150‑1 (MQ=255)
                                                                                                                                             cggggccgAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGAACCTGTACCGTCGCGGTGGCTc     <  2:180653/150‑1 (MQ=255)
                                                                                                                                              ggggccgAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGAACCTGTACCGTCGCGGTGGCTCg    <  1:5239754/150‑1 (MQ=255)
                                                                                                                                                ggccgAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGAACCTGTACCGTCGCGGTGGCTCggt  >  1:7713895/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GGCCAAGGCCCTGGGGGCGGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCGGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGAACCTGTACCGTCGCGGTGGCTCGGT  >  NC_008463/4707640‑4707933

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑