Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 4,760,743 A→G L73P (CTT→CCT)  PA14_RS21850 ← HNH endonuclease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084634,760,7430AG99.7% 1426.6 / NA 366L73P (CTT→CCT) PA14_RS21850HNH endonuclease
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (175/190);  minor base T (0/1);  total (175/191)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 373 total aligned reads displayed.

ATCTCCCGGACACCACTACGCCAGTAGTTGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAAGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCTGGCTTAAGGATGGCGCTGTATCGCCTT  >  NC_008463/4760596‑4760889
                                                                                                                                                   |                                                                                                                                                  
  ctcCCGGACACCACTACGCCAGTAGTTGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTc                                                                                                                                                >  1:8799586/1‑150 (MQ=255)
  ctcCCGGACACCACTACGCCAGTAGTTGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTc                                                                                                                                                <  1:689471/150‑1 (MQ=255)
  ctcCCGGACACCACTACGCCAGTAGTTGCTGGCCTTCGATGGAGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTc                                                                                                                                                <  1:1855139/150‑1 (MQ=255)
       ggACACCACTACGCCAGTAGTTGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTcagggtcagggt                                                                                                                                           <  2:2306512/150‑1 (MQ=255)
         acacCACTACGCCAGTAGTTGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTaa                                                                                                                                         <  1:5559046/150‑1 (MQ=255)
         acacCACTACGCCAGTAGTTGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTaa                                                                                                                                         >  2:3151153/1‑150 (MQ=255)
         acacCACTACGCCAGTAGTTGCTGGACTTCGATGGCGGAATGGGCTCTATGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTAGATCAACGCGAAGATATCACCTTTCGAGCTTTCAGGGTCAGGGTaa                                                                                                                                         >  2:4602216/1‑150 (MQ=255)
          caccacTACGCCAGTAGTTGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTaaa                                                                                                                                        >  2:3894587/1‑150 (MQ=255)
           accacTACGCCAGTAGTTGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAAc                                                                                                                                       <  1:10764868/150‑1 (MQ=255)
            ccacTACGCCAGTAGTTGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTCGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAAcc                                                                                                                                      >  2:2502688/1‑150 (MQ=255)
              acTACGCCAGTAGTTGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCtt                                                                                                                                    >  2:3533862/1‑150 (MQ=255)
               cTACGCCAGTAGTTGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTg                                                                                                                                   >  2:5898891/1‑150 (MQ=255)
                tACGCCAGTAGTTGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGc                                                                                                                                  <  1:9866140/150‑1 (MQ=255)
                tACGCCAGTAGTTGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCGTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCGCGATCAACGCGAAGAGATCTCCTGTCGAGCTTTCAGGGTCTGGGGACACCTTGc                                                                                                                                  >  2:6631102/1‑150 (MQ=255)
                tACGCCAGTAGTTGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCGATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGACGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGc                                                                                                                                  >  1:3666143/1‑150 (MQ=255)
                 aCGCCAGTAGTTGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGcc                                                                                                                                 <  1:5349424/150‑1 (MQ=255)
                   cccAGTAGTTGCTGGCCTTCGATGTCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGACAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAACCCTTGCCGa                                                                                                                               <  2:515746/149‑1 (MQ=255)
                       gtagtTGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATAcg                                                                                                                           <  2:4945643/150‑1 (MQ=255)
                        tagtTGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATAcgc                                                                                                                          >  1:2367445/1‑150 (MQ=255)
                           ttGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCa                                                                                                                       <  1:4901068/150‑1 (MQ=255)
                             gCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAAt                                                                                                                     <  1:7274682/150‑1 (MQ=255)
                               tGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATAc                                                                                                                   >  1:3960647/1‑150 (MQ=255)
                                ggCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATAcc                                                                                                                  <  1:9978900/150‑1 (MQ=255)
                                 gCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAATCTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCa                                                                                                                 <  2:6305964/150‑1 (MQ=255)
                                 gCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCa                                                                                                                 <  2:721890/150‑1 (MQ=255)
                                 gCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCa                                                                                                                 <  1:8424162/150‑1 (MQ=255)
                                   cTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCaaa                                                                                                               >  1:2414149/1‑150 (MQ=255)
                                    ttCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAg                                                                                                              <  1:7609285/150‑1 (MQ=255)
                                    ttCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAg                                                                                                              <  1:1536157/150‑1 (MQ=255)
                                      cGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTa                                                                                                            <  2:6046075/150‑1 (MQ=255)
                                       gATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTaa                                                                                                           <  2:4190303/150‑1 (MQ=255)
                                        aTGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAAt                                                                                                          <  2:7508351/150‑1 (MQ=255)
                                        aTGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAAt                                                                                                          <  1:7391239/150‑1 (MQ=255)
                                        aTGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAAt                                                                                                          <  1:3110460/150‑1 (MQ=255)
                                         tGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATg                                                                                                         >  1:584910/1‑150 (MQ=255)
                                         tGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATg                                                                                                         <  1:1128317/150‑1 (MQ=255)
                                         tGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATg                                                                                                         >  2:9183272/1‑150 (MQ=255)
                                            cGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCgg                                                                                                      <  1:4547206/150‑1 (MQ=255)
                                              gAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCt                                                                                                    <  2:10301709/150‑1 (MQ=255)
                                              gAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTGTCAGGGTCAGGGTAAACCTTGACGATACGCGCAATACCAAAGTAATGCGGCt                                                                                                    >  2:1430305/1‑150 (MQ=255)
                                               aaTGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTg                                                                                                   >  1:4009819/1‑150 (MQ=255)
                                                aTGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGa                                                                                                  <  1:9577167/150‑1 (MQ=255)
                                                 tGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAg                                                                                                 <  2:2208182/150‑1 (MQ=255)
                                                    gCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCAc                                                                                              >  2:5119163/1‑150 (MQ=255)
                                                    gCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCAc                                                                                              >  1:6545226/1‑150 (MQ=255)
                                                        tAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAGTGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGAAATGCGGCTGAGCACTGAg                                                                                          >  2:8496653/1‑150 (MQ=255)
                                                            tAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGgcgc                                                                                      <  2:4727275/150‑1 (MQ=255)
                                                             aGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCt                                                                                     >  2:5638972/1‑150 (MQ=255)
                                                             aGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCt                                                                                     >  2:1614712/1‑150 (MQ=255)
                                                                tATTCCCTTCTATTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGAt                                                                                  <  1:1792104/150‑1 (MQ=255)
                                                                      cttcttTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCaa                                                                            <  2:2910685/150‑1 (MQ=255)
                                                                         cttTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAgg                                                                         >  2:5291002/1‑150 (MQ=255)
                                                                         cttTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAgg                                                                         >  2:2649358/1‑150 (MQ=255)
                                                                           tttGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGAc                                                                       >  2:10707197/1‑150 (MQ=255)
                                                                             tGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACtt                                                                     >  2:9458904/1‑150 (MQ=255)
                                                                             tGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACtt                                                                     <  2:578475/150‑1 (MQ=255)
                                                                                 aGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCACCGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCt                                                                 <  2:6811154/150‑1 (MQ=255)
                                                                                 aGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCt                                                                 >  2:10744536/1‑150 (MQ=255)
                                                                                   gACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGt                                                               >  1:5118720/1‑150 (MQ=255)
                                                                                    aCTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTc                                                              >  1:2834738/1‑150 (MQ=255)
                                                                                    aCTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTc                                                              <  1:2781250/150‑1 (MQ=255)
                                                                                     ctgcctgcTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCa                                                             <  1:3193816/150‑1 (MQ=255)
                                                                                      tgcctgcTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAg                                                            >  1:2483767/1‑150 (MQ=255)
                                                                                        cctgcTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCt                                                          <  1:776798/150‑1 (MQ=255)
                                                                                        cctgcTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCt                                                          >  2:1132359/1‑150 (MQ=255)
                                                                                           gcTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGc                                                       >  1:631414/1‑150 (MQ=255)
                                                                                            cTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGcc                                                      >  1:1246765/1‑150 (MQ=255)
                                                                                              cAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCt                                                    <  1:10491105/150‑1 (MQ=255)
                                                                                                 aTGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTgt                                                 <  2:9640448/150‑1 (MQ=255)
                                                                                                 aTGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTgt                                                 <  2:9286368/150‑1 (MQ=255)
                                                                                                  tGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTgta                                                <  1:3419296/150‑1 (MQ=255)
                                                                                                       gAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTaaa                                           >  1:816551/1‑150 (MQ=255)
                                                                                                         aaaCTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAacc                                         <  2:5990973/150‑1 (MQ=255)
                                                                                                              ctcGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTACACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCTGCTTGCCCTTGTAGTAAACCACCTg                                    >  2:10183677/1‑150 (MQ=255)
                                                                                                                cGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGcc                                  >  2:6936994/1‑149 (MQ=255)
                                                                                                                cGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCa                                  >  1:10049120/1‑150 (MQ=255)
                                                                                                                cGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCa                                  >  1:932389/1‑150 (MQ=255)
                                                                                                                    cAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATCCCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCAcccc                              >  2:7022/1‑150 (MQ=255)
                                                                                                                    cAACGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCAcccc                              >  1:7860654/1‑150 (MQ=255)
                                                                                                                     aaCGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCt                             <  2:360798/150‑1 (MQ=255)
                                                                                                                     aaCGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCt                             <  1:2001572/150‑1 (MQ=255)
                                                                                                                      aCGCGAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCTg                            <  1:4092089/150‑1 (MQ=255)
                                                                                                                        gcgAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCTGGc                          >  1:10168330/1‑150 (MQ=255)
                                                                                                                          gAAGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCTGGCtt                        <  1:6036283/150‑1 (MQ=255)
                                                                                                                          gAAGAGATCACATTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCCCCTGCACCCCTGGCtt                        <  2:789065/150‑1 (MQ=255)
                                                                                                                           aaGAGATCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCTGGCTTa                       >  2:6536798/1‑150 (MQ=255)
                                                                                                                             gagaACACCTGACGCGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCTGGCTTAAg                     <  2:8193518/150‑1 (MQ=255)
                                                                                                                               gaTCACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCTGGCTTAAGGa                   >  2:8275195/1‑150 (MQ=255)
                                                                                                                                  cACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCTGGCTTAAGGATgg                <  1:5902263/150‑1 (MQ=255)
                                                                                                                                  cACCTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCTGGCTTAAGGATgg                <  2:3344895/150‑1 (MQ=255)
                                                                                                                                    ccTTTCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCTGGCTTAAGGATGgcg              <  2:7195214/150‑1 (MQ=255)
                                                                                                                                        tCGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCTGGCTTAAGGATGGCGCTGt          <  1:8968101/150‑1 (MQ=255)
                                                                                                                                         cGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCTGGCTTAAGGATGGCGCTGTa         <  2:190301/150‑1 (MQ=255)
                                                                                                                                         cGAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCTGGCTTAAGGATGGCGCTGTa         <  2:872951/150‑1 (MQ=255)
                                                                                                                                          gAGCTTTCAGTGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTATTGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCTGGCATAAGGATGGCGCTGTAt        <  1:2250944/150‑1 (MQ=255)
                                                                                                                                          gAGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCTGGCTTAAGGATGGCGCTGTAt        >  2:3838578/1‑150 (MQ=255)
                                                                                                                                           aGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCTGGCTTAAGGATGGCGCTGTATc       >  1:3558499/1‑150 (MQ=255)
                                                                                                                                           aGCTTTCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCTGGCTTAAGGATGGCGCTGTATc       >  1:4769322/1‑150 (MQ=255)
                                                                                                                                               ttCAGGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCTGGCTTAAGGATGGCGCTGTATCGCCt   <  1:921207/150‑1 (MQ=255)
                                                                                                                                                tcagggtcagggTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCTGGCTTAAGGATGGCGCTGTATCGCCtt  <  1:3883516/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
ATCTCCCGGACACCACTACGCCAGTAGTTGCTGGCCTTCGATGGCGGAATGGGCTCTAGGTAGCTATTCCCTTCTTTTGCCAGGACTGCCTGCTCAAATGGCTGAAAACTCTCGATCAACGCGAAGAGATCACCTTTCGAGCTTTCAAGGTCAGGGTAAACCTTGCCGATACGCGCAATACCAAAGTAATGCGGCTGAGCACTGAGGCGCTGATCAGCAAAGGACTTATCTGTCAGCTTGCCCTTGTAGTAAACCACCTGCACCCCTGGCTTAAGGATGGCGCTGTATCGCCTT  >  NC_008463/4760596‑4760889

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑