Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 5,198,405 T→C intergenic (+31/+37) PA14_RS23800 → / ← mdpA ABC transporter substrate‑binding protein/metallopeptidase MdpA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084635,198,4050TC100.0% 1418.2 / NA 371intergenic (+31/+37)PA14_RS23800/mdpAABC transporter substrate‑binding protein/metallopeptidase MdpA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (176/195);  total (176/195)

Only 100 of 371 total aligned reads displayed.

TGCCCTGGTTGCCGCTGGCGCATCCGTTGATGTTCGACGCGCAACGCAAGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCTTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTCGGTTGGGTGAACCAGCGTGGTCCTTCCTCCGTCATGTAGAAGTGATCCTCCAGGCGAATGCCGAACTCGC  >  NC_008463/5198259‑5198551
                                                                                                                                                  |                                                                                                                                                  
 gCCCTGGTTGCCGCTGGCGCATCCGTTGATGTTCGACGCGCAACGCAAGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGc                                                                                                                                                <  2:6720242/150‑1 (MQ=255)
  cccTGGTTGCCGCTGGCGCATCCGTTGATGTTCGACGCGCAACGCAAGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCATGcc                                                                                                                                               >  1:1053662/1‑150 (MQ=255)
     tGGTTGCCGCTGGCGCATCCGTTGATGTTCGACGCGCAACGCAAGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTc                                                                                                                                            >  2:10535087/1‑150 (MQ=255)
     tGGTTGCCGCTGGCGCATCCGTTGATGTTCGACGCGCAACGCAAGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCGGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTc                                                                                                                                            >  2:8151122/1‑150 (MQ=255)
       ggtGCCGCTGGCGCATCCGTTGATGTTCGACGCGCAACGCAAGAACGTCAGCGGCTACCGCATGAGCCCGTTGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTccc                                                                                                                                          <  1:1946124/148‑1 (MQ=255)
         tGCCGCTGGCGCATCCGTTGATGTTCGACGCGCAACGCAAGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCcgc                                                                                                                                        <  2:3786439/150‑1 (MQ=255)
         tGCCGCTGGCGCATCCGTTGATGTTCGACGCGCAACGCAAGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCcgc                                                                                                                                        <  1:2472765/150‑1 (MQ=255)
           ccGCTGGCGCATCCGTTGATGTTCGACGCGCAACGCAAGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGt                                                                                                                                      <  1:4757295/150‑1 (MQ=255)
            cGCTGGCGCATCCGTTGATGTTCGACGCGCAACGCAAGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTg                                                                                                                                     <  1:9572958/150‑1 (MQ=255)
              cTGGCGCATCCGTTGATGTTCGACGCGCAACGCAAGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTgcg                                                                                                                                   <  2:10826063/150‑1 (MQ=255)
              cTGGCGCATCCGTTGATGTTCGACGCGCAACGCAAGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTgcg                                                                                                                                   <  2:4664490/150‑1 (MQ=255)
               tGGCGCATCCGTTGATGTTCGACGCGCAACGCAAGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTgcgg                                                                                                                                  >  1:1180780/1‑150 (MQ=255)
                ggCGCATCCGTTGATGTTCGACGCGCAACGCAAGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTgcggc                                                                                                                                 >  1:6177385/1‑150 (MQ=255)
                ggCGCATCCGTTGATGTTCGACGCGCAACGCAAGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCGTACCGCGTgcggc                                                                                                                                 >  2:1846269/1‑150 (MQ=255)
                   gcATCCGTTGATGTTCGACGCGCAACGCCAGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAGGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGtt                                                                                                                              >  1:1347868/1‑150 (MQ=255)
                    cATCCGTTGATGTTCGACGCGCAACGCAAGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTa                                                                                                                             >  2:3407044/1‑150 (MQ=255)
                    cATCCGTTGATGTTCGACGCGCAACGCAAGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTACGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGACGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTa                                                                                                                             >  1:6709188/1‑150 (MQ=255)
                     aTCCGTTGATGTTCGACGCGCAACGCAAGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAATCCCTGGCCCGTGCCTTACCGCGTGCGGCGTTAt                                                                                                                            >  1:2125244/1‑150 (MQ=255)
                       ccGTTGATGTTCGACGCGCAACGCAAGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCATGCCATCCCGCGGGCGGCGGTAtag                                                                                                                          >  1:9252756/1‑148 (MQ=255)
                        cGTTGATGTTCGACGCGCAACGCAAGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGt                                                                                                                         >  2:5737933/1‑150 (MQ=255)
                         gTTGATGTTCGACGCGCAACGCAAGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGtt                                                                                                                        <  2:7590658/150‑1 (MQ=255)
                          atGATGTTCGACGCGCAACGCAAGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCGGCGACGCACGCGGGCAGCCCTGGCCCTTGCCGTCCCGCGTGCGGCGTTATCGTTc                                                                                                                       >  2:1722217/2‑150 (MQ=255)
                            gATGTTCGACGCGCAACGCAAGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTcgg                                                                                                                     >  2:5152438/1‑150 (MQ=255)
                              tGTTCGACGCGCAACGCAAGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTcggcc                                                                                                                   <  1:5493342/150‑1 (MQ=255)
                               gTTCGACGCGCAACGCAAGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTcggccg                                                                                                                  <  2:7951189/150‑1 (MQ=255)
                               gTTCGACGCGCAACGCAAGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGGTATCGTTccgccg                                                                                                                  >  1:6916935/1‑150 (MQ=255)
                                  cGACGCGCAACGCAAGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCt                                                                                                               <  2:1570284/150‑1 (MQ=255)
                                  cGACGCGCAACGCAAGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCt                                                                                                               <  2:4235115/150‑1 (MQ=255)
                                   gACGCGCAACGCAAGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTc                                                                                                              >  1:7061140/1‑150 (MQ=255)
                                     cgcgCAACGCAAGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAg                                                                                                            <  1:8420098/150‑1 (MQ=255)
                                      gcgcAACGCAAGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAgc                                                                                                           <  2:3056006/150‑1 (MQ=255)
                                       cgcaacgcaaGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGGAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGGTATCGTTCGGCCGGCTCAgcc                                                                                                          >  1:3547651/1‑150 (MQ=255)
                                       cgcaacgcaaGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAgcc                                                                                                          <  2:6001454/150‑1 (MQ=255)
                                         caacgcaaGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAgccgc                                                                                                        >  2:6412611/1‑150 (MQ=255)
                                         caacgcaaGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAgccgc                                                                                                        <  2:8966502/150‑1 (MQ=255)
                                          aacgcaaGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAgccgcc                                                                                                       <  1:5230400/150‑1 (MQ=255)
                                           acgcaaGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGccgccg                                                                                                      <  2:10721209/150‑1 (MQ=255)
                                             gcaaGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAg                                                                                                    <  2:8075752/150‑1 (MQ=255)
                                             gcaaGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAg                                                                                                    >  1:9682526/1‑150 (MQ=255)
                                               aaGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGc                                                                                                  <  1:1531784/150‑1 (MQ=255)
                                                aGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGcc                                                                                                 >  1:3760343/1‑150 (MQ=255)
                                                  aaCGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGa                                                                                               <  2:11047269/150‑1 (MQ=255)
                                                      tCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCGTGCCGTCCCGCGTGCGGCGGTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAggg                                                                                           >  1:5087583/1‑150 (MQ=255)
                                                       cAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGa                                                                                          <  1:10994694/150‑1 (MQ=255)
                                                        aGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGAt                                                                                         <  1:3671006/150‑1 (MQ=255)
                                                           ggCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGGAGCCCAGGCCCGTGGCTTGCCGCGTGGGGGGGTTTCGTGGGGCCGGCGCAGCCGCCGAGGCCGACGGGGtcgt                                                                                      >  1:5609834/1‑150 (MQ=255)
                                                           ggCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGAtcgt                                                                                      <  1:2878136/150‑1 (MQ=255)
                                                           ggCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTGCGGCCGGCTCAGCCGCCGAGGCCGAAGGGAtcgt                                                                                      >  2:6140680/1‑150 (MQ=255)
                                                                ccGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATg                                                                                 <  1:3380577/150‑1 (MQ=255)
                                                                ccGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATg                                                                                 <  1:1073292/150‑1 (MQ=255)
                                                                 cGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATgg                                                                                >  2:909663/1‑150 (MQ=255)
                                                                 cGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATgg                                                                                <  1:6477195/150‑1 (MQ=255)
                                                                 cGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGGTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATgg                                                                                >  1:4509976/1‑150 (MQ=255)
                                                                  gCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATgga                                                                               <  1:9120151/150‑1 (MQ=255)
                                                                   gaTGAGCCCGATTTAGGCGCGGGACTTTTAGCGGGTGAAGCTGGACTGATCGCTGCGCCGCAACCGGGAAACCCATGCCCATTCCATCCCCCGTGCGGCGTTATCGGTCGGCCCGCTCAACCCCCCACGCCGAACGGATCGTCGAAggac                                                                              >  2:2077797/2‑150 (MQ=255)
                                                                   cATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCAGGCCCTTGCCTTCCCCCGTGCGGCGTTATCGTTAGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATggac                                                                              >  1:6582734/1‑150 (MQ=255)
                                                                      gAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATggacgga                                                                           <  1:9928252/150‑1 (MQ=255)
                                                                       aGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATggacggac                                                                          >  2:8425845/1‑150 (MQ=255)
                                                                       aGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATggacggac                                                                          <  1:5431925/150‑1 (MQ=255)
                                                                         cccGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTc                                                                        <  2:10135386/150‑1 (MQ=255)
                                                                             aTGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGGTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGCCTCGGtt                                                                    >  1:10044082/1‑150 (MQ=255)
                                                                             aTGTCGGCGCGGGACATTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTCGGtt                                                                    <  2:6497983/150‑1 (MQ=255)
                                                                                       gggACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTCGGTTGGGTGAACCa                                                          <  1:7925734/150‑1 (MQ=255)
                                                                                         gACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGGGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATAGTCGATGGACGGACTCGGTTGGGTGAACCAGc                                                        >  2:8295976/1‑150 (MQ=255)
                                                                                          aCTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGGAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTTTAGTTAGGCCGGGTGAGCCGCCGCGGGCGAAGGGATCGTCGATGGACGGACTCGGTTTGGTGAACCAGCg                                                       >  1:2226775/1‑150 (MQ=255)
                                                                                          aCTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTCGGTTGGGTGAACCAGCg                                                       <  1:4231645/150‑1 (MQ=255)
                                                                                          aCTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACGCGGGTGGGTGAACCAGCg                                                       >  2:1412636/1‑150 (MQ=255)
                                                                                             tttCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTCGGTTGGGTGAACCAGCGTgg                                                    <  1:5911/150‑1 (MQ=255)
                                                                                                   gggTGAAGCTGGACTGATCGCTGCGCCGAACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTCGGTTGGGTGAACCAGCGTGGtccttc                                              >  2:548074/1‑150 (MQ=255)
                                                                                                     gTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTCGGTTGGGTGAACCAGCGTGGtccttcct                                            >  2:780915/1‑150 (MQ=255)
                                                                                                       gAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCAGGCCCGTGCCATCCCGCGTGCGGCGTTATCGTTAGGGCGGCTCAGCCCCCGAGGCCGAAGGGATAGGCGATGGACGGACTCGGTTTGGTGAACCAGCGTGGGGCTtactcc                                          >  1:2346326/1‑150 (MQ=255)
                                                                                                        aaGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTCGGTTGGGTGAACCAGCGTGGTCCTTCCTCCg                                         <  2:8535643/150‑1 (MQ=255)
                                                                                                        aaGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTCGGTTGGGTGAACCAGCGTGGTCCTTCCTCCg                                         <  1:4297628/150‑1 (MQ=255)
                                                                                                         aGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTCGGTTGGGTGAACCAGCGGGGTCCTTCCTCCGt                                        >  2:7071964/1‑150 (MQ=255)
                                                                                                          gCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGGTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTCGGTTGGGTGAACCAGCGTGGTCCTTCCTCCGTc                                       <  2:864339/150‑1 (MQ=255)
                                                                                                          gCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCAGGCCCATGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGTTCGTCGATGGACGGACTCGGTTGGGTGTACCAGCGTGGTCCTTACTCCGTc                                       >  1:8244475/1‑150 (MQ=255)
                                                                                                             ggACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCCGCCGCCGAGGCCGTAGGGATCGTCGATGGACGGACGCGGTTGGGTGTACCAGCGTGGTCCTTCCTCCGTCATg                                    >  2:3161590/1‑150 (MQ=255)
                                                                                                             ggACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTCGGTTGGGTGAACCAGCGTGGTCCTTCCTCCGTCATg                                    <  1:7337255/150‑1 (MQ=255)
                                                                                                             ggACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTCGGTTGGGTGAACCAGCGTGGTCCTTCCTCCGTCATg                                    <  1:1806938/150‑1 (MQ=255)
                                                                                                               aCTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTCGGTTGGGTGAACCAGCGTGGTCCTTCCTCCGTCATGTa                                  <  1:7668523/150‑1 (MQ=255)
                                                                                                                   aTCGCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTCGGTTGGGTGAACCAGCGTGGTCCTTCCTCCGTCATGTAGAAg                              <  1:2718136/150‑1 (MQ=255)
                                                                                                                      gCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTCGGTTGGGTGAACCAGCGTGGTCCTTCCTCCGTCATGTAGAAGTGa                           <  2:7160308/150‑1 (MQ=255)
                                                                                                                      gCTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTCGGTTGGGTGAACCAGCGTGGTCCTTCCTCCGTCATGTAGAAGTGa                           >  2:9631714/1‑150 (MQ=255)
                                                                                                                       cTGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTCGGTTGGGTGAACCAGCGTGGTCCTTCCTCCGTCATGTAGAAGTGAt                          <  2:3635303/150‑1 (MQ=255)
                                                                                                                        tGCGCCGCACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTCGGTTGGGTGAACCAGCGTGGTCCTTCCTCCGTCATGTAGAAGTGAtc                         >  2:272806/1‑150 (MQ=255)
                                                                                                                           gccgcACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTCGGTTGGGTGAACCAGCGTGGTCCTTCCTCCGTCATGTAGAAGTGAtcctc                      <  2:3976690/150‑1 (MQ=255)
                                                                                                                            ccgcACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTCGGTTGGGTGAACCAGCGTGGTCCTTCCTCCGTCATGTAGAAGTGAtcctcc                     <  1:8778708/150‑1 (MQ=255)
                                                                                                                               cACGCGGGAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTCGGTTGGGTGAACCAGCGTGGTCCTTCCTCCGTCATGTAGAAGTGATCCTCCAgg                  >  1:9454354/1‑150 (MQ=255)
                                                                                                                                     ggAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTCGGTTGGGTGAACCAGCGTGGTCCTTCCTCCGTCATGTAGAAGTGATCCTCCAGGCGAATg            >  2:5099757/1‑150 (MQ=255)
                                                                                                                                     ggAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTCGGTTGGGTGAACCAGCGTGGTCCTTCCTCCGTCATGTAGAAGTGATCCTCCAGGCGAATg            >  1:8853218/1‑150 (MQ=255)
                                                                                                                                      gAAGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTCGGTTGGGTGAACCAGCGTGGTCCTTCCTCCGTCATGTAGAAGTGATCCTCCAGGCGAATGc           >  1:8182227/1‑150 (MQ=255)
                                                                                                                                       aaGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTCGGTTGGGTGACCCAGCGTGGTCCTTCCTCCGTCATGTAGAAGTGATCCTCCAGGCGAATGcc          >  2:7844605/1‑150 (MQ=255)
                                                                                                                                       aaGCCCTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTCGGTTGGGTGAACCAGCGTGGTCCTTCCTCCGTCATGTAGAAGTGATCCTCCAGGCGAATGcc          <  1:757908/150‑1 (MQ=255)
                                                                                                                                       aaGCCCTGGCCCGTGCCGTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTCGGTTGGGTGAACCAGCGTGGTCCTTCCTCCGTCATGTAGAAGTGATCCTCCAGGCGAATGcc          <  2:5807336/150‑1 (MQ=255)
                                                                                                                                          cccTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTCGGTTGGGTGAACCAGCGTGGTCCTTCCTCCGTCATGTAGAAGTGATCCTCCAGGCGAATGCCGaa       <  2:9336527/150‑1 (MQ=255)
                                                                                                                                           ccTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTCGGTTGGGTGAACCAGCGTGGTCCTTCCTCCGTCATGTAGAAGTGATCCTCCAGGCGAATGCCGAAc      >  2:4749510/1‑150 (MQ=255)
                                                                                                                                            cTGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTCGGTTGGGTGAACCAGCGTGGTCCTTCCTCCGTCATGTAGAAGTGATCCTCCAGGCGAATGCCGAACt     <  1:10122961/150‑1 (MQ=255)
                                                                                                                                             tGGCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTCGGTTGGGGGACCCAGCGTGGTCCTTCCTCCGTCATGTAGAAGGGATCCTCCAGGCGAATGCCGAACTc    >  2:6870525/1‑150 (MQ=255)
                                                                                                                                               gCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTCGGTTGGGTGAACCAGCGTGGTCCTTCCTCCGTCATGTAGAAGTGATCCTCCAGGCGAATGCCGAACTCGc  >  1:9201753/1‑150 (MQ=255)
                                                                                                                                               gCCCTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTCGGTTGGGTGAACCAGCGTGGTCCTTCCTCCGTCATGTAGAAGTGATCCTCCAGGCGAATGCCGAACTCGc  <  2:8018329/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
TGCCCTGGTTGCCGCTGGCGCATCCGTTGATGTTCGACGCGCAACGCAAGAACGTCAGCGGCTACCGCATGAGCCCGATGTCGGCGCGGGACTTTTCGCGGGTGAAGCTGGACTGATCGCTGCGCCGCACGCGGGAAGCCCTGGCCTTTGCCTTCCCGCGTGCGGCGTTATCGTTCGGCCGGCTCAGCCGCCGAGGCCGAAGGGATCGTCGATGGACGGACTCGGTTGGGTGAACCAGCGTGGTCCTTCCTCCGTCATGTAGAAGTGATCCTCCAGGCGAATGCCGAACTCGC  >  NC_008463/5198259‑5198551

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑