Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 732,660 G→A intergenic (+114/‑426) tyrS → / → PA14_RS03450 tyrosine‑‑tRNA ligase/16S ribosomal RNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_008463732,6600GA97.9% 336.5 / ‑1.8 93intergenic (+114/‑426)tyrS/PA14_RS03450tyrosine‑‑tRNA ligase/16S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (56/35);  total (57/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 102 total aligned reads displayed.

GGCTTTCGCGCGCATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGGGTGGTGAAGCGCTCCGGCAGGTTGTTCGCCGCAGCGGTTCGGTCCCGAAAGGGGCTCGAAA  >  NC_008463/732513‑732721
                                                                                                                                                   |                                                             
ggCTTTCGCGCGCATCACGCTGTAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATCATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGagt                                                             <  2:1631291/150‑1 (MQ=255)
ggCTGTCGCGCGCATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGagt                                                             <  2:9338542/150‑1 (MQ=255)
  cTTTCGCGCGCATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGagtgg                                                           <  1:7982862/150‑1 (MQ=255)
  cTTTCGCGCGCATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGagtgg                                                           <  1:964198/150‑1 (MQ=255)
   tttCGCTCGCATGTCGCTGATAGCTGAATTATTCTTTCTAATAAGTTTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCTGCGACGACGATGATTATATGCTTGAATATCAAGGATTTACGGATTCTCTCCCAGGagtggt                                                          <  1:6179329/150‑1 (MQ=14)
   tttCGCGCGCATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTATATGCTTGAAAATCAAGGATTTACGGATTCTCTCACAGGagtggt                                                          >  2:5674306/1‑150 (MQ=255)
   tttCGCGCGCATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGagtggt                                                          >  2:4371123/1‑150 (MQ=255)
   tttCGCGCGCATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGagtggt                                                          >  1:8216583/1‑150 (MQ=255)
   tttCGCGCGCATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGagtggt                                                          >  1:8794102/1‑150 (MQ=255)
    ttCGCGCGCATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAgtggtg                                                         <  1:10664390/150‑1 (MQ=255)
     tCGCGCGCATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGa                                                        <  2:2919/150‑1 (MQ=255)
      cgcgcgCATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGaa                                                       <  2:3123264/150‑1 (MQ=255)
       gcgcgcATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAg                                                      >  2:7820506/1‑150 (MQ=255)
       gcgcgcATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAg                                                      <  1:735137/150‑1 (MQ=255)
       gcgcgcATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAg                                                      >  2:7820370/1‑150 (MQ=255)
       gcgcgcATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAg                                                      >  2:7816444/1‑150 (MQ=255)
        cgcgcATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAgc                                                     <  1:1000209/150‑1 (MQ=255)
         gcgcATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAgcg                                                    >  2:3035941/1‑150 (MQ=255)
          cgcATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAgcgc                                                   <  2:372477/150‑1 (MQ=255)
            cATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATACTGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTc                                                 <  2:5806774/150‑1 (MQ=255)
            cATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAACATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTc                                                 >  1:9265090/1‑150 (MQ=255)
            cATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTc                                                 >  1:2817493/1‑150 (MQ=255)
            cATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTc                                                 >  1:1476629/1‑150 (MQ=255)
            cATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTAGACGCCATTTTCTATCTCCCTATAATGCGCACCACTACCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTc                                                 >  1:1474659/1‑150 (MQ=255)
              tCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCg                                               >  1:892152/1‑150 (MQ=255)
              tCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCg                                               >  1:1319289/1‑150 (MQ=255)
               cACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCgg                                              >  1:9689514/1‑150 (MQ=255)
               cACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCgg                                              <  2:7458610/150‑1 (MQ=255)
               cACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCgg                                              >  2:2603732/1‑150 (MQ=255)
                aCGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGc                                             <  1:8485610/150‑1 (MQ=255)
                aCGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGc                                             <  2:870658/150‑1 (MQ=255)
                aCGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCATATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGAGCTCCGGc                                             <  2:4043289/150‑1 (MQ=255)
                 cGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGct                                            >  2:10995548/1‑149 (MQ=255)
                 cGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCa                                            <  2:1507155/150‑1 (MQ=255)
                 cGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCa                                            <  2:909320/150‑1 (MQ=255)
                  gCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAg                                           >  2:6661300/1‑150 (MQ=255)
                  gCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAg                                           >  1:6908585/1‑150 (MQ=255)
                   cTGAAAGCTGAATAATTCTTTCACATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAgg                                          <  1:418167/150‑1 (MQ=255)
                   cTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAgg                                          >  1:7313082/1‑150 (MQ=255)
                   cTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAgg                                          >  1:7313326/1‑150 (MQ=255)
                   cTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAgg                                          <  1:4615811/150‑1 (MQ=255)
                   cTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAgg                                          <  1:418836/150‑1 (MQ=255)
                   cTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAgg                                          >  2:6607708/1‑150 (MQ=255)
                   cTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAgg                                          >  1:3069991/1‑150 (MQ=255)
                   cTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAgg                                          >  1:9409084/1‑150 (MQ=255)
                   cTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTATCTCCCAGGAGTGGTGAAGCGCTcaggcagg                                          >  1:8422553/1‑150 (MQ=255)
                    tGAAAGCTTAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGgt                                         <  1:5333389/150‑1 (MQ=255)
                    tGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGgt                                         >  1:5022210/1‑150 (MQ=255)
                    tGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGgt                                         <  2:10343707/150‑1 (MQ=255)
                    tGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGgt                                         >  2:8135831/1‑150 (MQ=255)
                     gAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGgtt                                        >  1:4073786/1‑150 (MQ=255)
                     gAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGgtt                                        >  1:2046427/1‑150 (MQ=255)
                     gAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGgtt                                        >  1:2828208/1‑150 (MQ=255)
                      aaaGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGgttg                                       >  2:1583543/1‑150 (MQ=255)
                      aaaGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGgttg                                       >  1:5749857/1‑150 (MQ=255)
                      aaaGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGgttg                                       >  1:2019066/1‑150 (MQ=255)
                      aaaGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGgttg                                       >  1:7553785/1‑150 (MQ=255)
                      aaaGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGgttg                                       >  1:6006299/1‑150 (MQ=255)
                      aaaGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTAACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGgttg                                       >  2:2759184/1‑150 (MQ=255)
                      aaaGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAAGGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGgttg                                       >  2:1583825/1‑150 (MQ=255)
                       aaGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGgttgt                                      >  1:2613450/1‑150 (MQ=38)
                       aaGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGgttgt                                      >  1:4936469/1‑150 (MQ=38)
                        aGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGgttgtt                                     <  1:10197653/150‑1 (MQ=38)
                        aGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGgttgtt                                     >  1:10593952/1‑150 (MQ=38)
                        aGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGgttgtt                                     >  1:10605545/1‑150 (MQ=38)
                        aGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGgttgtt                                     >  1:10605666/1‑150 (MQ=38)
                        aGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGgttgtt                                     <  1:1276418/150‑1 (MQ=38)
                        aGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGgttgtt                                     >  1:7278934/1‑150 (MQ=38)
                        aGCTGAATAAGTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGgttgtt                                     <  2:10985735/150‑1 (MQ=25)
                         gCTGAATAATTCTTTCAATTAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGGTTGTTc                                    >  2:9467932/1‑150 (MQ=255)
                          cTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGGTTGTTcg                                   >  2:4473614/1‑150 (MQ=25)
                          cTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGGTTGTTcg                                   <  2:3730719/150‑1 (MQ=25)
                          cTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGGTTGTTcg                                   <  1:8828464/150‑1 (MQ=25)
                          cTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTAACTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATGTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGGTTGTTcg                                   <  1:8826618/150‑1 (MQ=255)
                           tGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGGTTGTTcgc                                  <  1:6054284/150‑1 (MQ=25)
                           tGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGGTTGTTcgc                                  <  1:6054223/150‑1 (MQ=25)
                            gAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGGTTGTTcgcc                                 <  1:8530760/150‑1 (MQ=21)
                            gAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGGTTGTTcgcc                                 <  1:650085/150‑1 (MQ=21)
                            gAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGGTTGTTcgcc                                 >  2:10545024/1‑150 (MQ=21)
                            gAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGGTTGTTcgcc                                 <  1:6143217/150‑1 (MQ=21)
                            gAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGGTTGTTcgcc                                 >  1:10850083/1‑150 (MQ=21)
                            gAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGGTTGTTcgcc                                 >  1:1607714/1‑150 (MQ=21)
                             aataatTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGGTTGTTcgccg                                >  1:7961360/1‑150 (MQ=18)
                             aataatTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGGTTGTTcgccg                                >  2:1303199/1‑150 (MQ=18)
                             aataatTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGGTTGTTcgccg                                >  2:8283835/1‑150 (MQ=18)
                             aataatTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGGTTGTTcgccg                                >  1:9623616/1‑150 (MQ=18)
                             aataatTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGGTTGTTcgccg                                <  2:6972924/150‑1 (MQ=18)
                             aataatTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGGTTGTTcgccg                                >  2:6718830/1‑150 (MQ=18)
                             aataatTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGGTTGTTcgccg                                >  1:182271/1‑150 (MQ=18)
                             aataatTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGGTTGTTcgccg                                >  1:8050879/1‑150 (MQ=18)
                              ataatTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCTCTCCGGCAGGTTGTTcgccgc                               >  2:432612/1‑150 (MQ=17)
                              ataatTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGGTTGTTcgccgc                               >  1:9345961/1‑150 (MQ=17)
                               taatTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGGTTGTTCGCCGCa                              >  1:1699768/1‑150 (MQ=17)
                               taatTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGGTTGTTCGCCGCa                              <  2:4917838/150‑1 (MQ=17)
                               caatTCTTTCACAAAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGGTTGTTCGCCGCa                              <  2:8605342/149‑1 (MQ=9)
                                aataggttCAAATAAGTGTTGACTCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGGGTGGTGAAGCGCTCCGGCAGGTTGTTCGCCGCAg                             >  2:1126466/7‑150 (MQ=0)
                                aatTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGGTTGTTCGCCGCAg                             >  1:5268172/1‑150 (MQ=14)
                                aatTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGGTTGTTCGCCGCAg                             <  1:5753336/150‑1 (MQ=14)
                                aatTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGAGTGGTGAAGCGCTCCGGCAGGTTGTTCGCCGCAg                             <  1:10238568/150‑1 (MQ=14)
                                                           gtttagaTCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGAAAATGCTTGAAAATCAAGGATTTACTGATTCTCTCCCAGGGGTGGTGAAGCGCTCCGGCAGGTTGTTCGCCGCAGCGGTTCGGTCCCGAAAGGGGCTCGaaa  <  2:3778489/144‑1 (MQ=0)
                                                                                                                                                   |                                                             
GGCTTTCGCGCGCATCACGCTGAAAGCTGAATAATTCTTTCAAATAAGTGTTGACGCCAGTTTCTATCTCCCTATAATGCGCACCACTCCCGGCGACGACGAAGAGTAAATGCTTGAAAATCAAGGATTTACGGATTCTCTCCCAGGGGTGGTGAAGCGCTCCGGCAGGTTGTTCGCCGCAGCGGTTCGGTCCCGAAAGGGGCTCGAAA  >  NC_008463/732513‑732721

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑