Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 5,866,730 T→G A733A (GCA→GCC)  PA14_RS26890 ← ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084635,866,7300TG100.0% 1875.3 / NA 490A733A (GCA→GCC) PA14_RS26890ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (303/187);  total (303/187)

Only 100 of 490 total aligned reads displayed.

GATGAGCGGATTATTGGCGGCGGATGTAAGACGCCGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCTGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGCCCATGGAGTGGCAGCTTCCGGACAGGCGATG  >  NC_008463/5866584‑5866876
                                                                                                                                                  |                                                                                                                                                  
  tGAGCGGATTATTGGCGGCGGATGTAAGACGCCGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGcc                                                                                                                                               >  1:6545910/1‑150 (MQ=255)
    aGCGGATTATTGGCGGCGGATGTAAGACGCCGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGt                                                                                                                                             <  1:7247912/150‑1 (MQ=255)
    aGCGGATTATTGGCGGCGGATGTAAGACGCCGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGt                                                                                                                                             >  2:4869539/1‑150 (MQ=255)
      cGGATTATTGGCGGCGGATGTAAGACGCCGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTcg                                                                                                                                           >  1:6828383/1‑150 (MQ=255)
        gATTATTGGCGGCGGATGTAAGACGCCGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTcgcg                                                                                                                                         <  2:2795221/150‑1 (MQ=255)
              tGGCGGCGGATGTAAGACGCCGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTACGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAg                                                                                                                                   <  1:2141682/150‑1 (MQ=255)
                gcggcgGATGTAAGACGCCGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGc                                                                                                                                 >  1:6039779/1‑150 (MQ=255)
                 cggcggATGTAAGACGCCGTTGAACGCGCGGATCGAAGATGTACAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCt                                                                                                                                <  2:5520511/150‑1 (MQ=255)
                       aTGTAAGACGCCGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAg                                                                                                                          >  2:7329441/1‑150 (MQ=255)
                       aTGTAAGACGCCGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAg                                                                                                                          >  1:163273/1‑150 (MQ=255)
                       aTGTAAGACGCCGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAg                                                                                                                          >  1:2420761/1‑150 (MQ=255)
                        tGTAAGACGCCGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAgg                                                                                                                         <  1:10297875/150‑1 (MQ=255)
                        tGTAAGACGCCGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAgg                                                                                                                         >  1:8275322/1‑150 (MQ=255)
                          tAAGACGCCGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCg                                                                                                                       >  1:9098381/1‑150 (MQ=255)
                            aGACGCCGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTg                                                                                                                     <  2:1289441/150‑1 (MQ=255)
                                gCCGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGcc                                                                                                                 <  1:1770568/150‑1 (MQ=255)
                                 ccGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGccc                                                                                                                >  1:3913530/1‑150 (MQ=255)
                                  cGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGcccc                                                                                                               >  2:594189/1‑150 (MQ=255)
                                  cGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGcccc                                                                                                               <  1:2998365/150‑1 (MQ=255)
                                       aaCGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCt                                                                                                          <  2:10650066/150‑1 (MQ=255)
                                       aaCGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCt                                                                                                          >  1:5779495/1‑150 (MQ=255)
                                        aCGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTc                                                                                                         >  2:2330098/1‑150 (MQ=255)
                                         cgcgcgGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTcg                                                                                                        >  2:2555097/1‑150 (MQ=255)
                                          gcgcgGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTcgc                                                                                                       >  2:4805716/1‑150 (MQ=255)
                                           cgcgGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTcgcg                                                                                                      >  1:3389763/1‑150 (MQ=255)
                                            gcgGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCgg                                                                                                     >  2:620317/1‑150 (MQ=255)
                                            gcgGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGGCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCgg                                                                                                     <  2:3846484/150‑1 (MQ=255)
                                                aTCGAAGATGTAAAAAGCTGTAAGGCAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCg                                                                                                 >  2:6979682/1‑150 (MQ=255)
                                                aTCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCg                                                                                                 >  2:3055933/1‑150 (MQ=255)
                                                 tCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGTAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGAGCGCCCCAGGCTCGCGGGTCGa                                                                                                >  2:5681659/1‑150 (MQ=255)
                                                 tCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGa                                                                                                <  2:7745472/150‑1 (MQ=255)
                                                  cGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGAc                                                                                               <  1:5031811/150‑1 (MQ=255)
                                                   gAAGATGTAAACAGCTGTAAGGAAGTTGTTCGCCGCGCCGCCCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCCGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGAcc                                                                                              <  1:1513068/150‑1 (MQ=255)
                                                   gAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGAcc                                                                                              >  1:1178591/1‑150 (MQ=255)
                                                   gAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGAcc                                                                                              >  1:8688975/1‑150 (MQ=255)
                                                    caGATGTAAACAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGAccc                                                                                             <  1:562172/149‑1 (MQ=255)
                                                    aaGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGAccc                                                                                             >  2:3237824/1‑150 (MQ=255)
                                                    aaGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGAccc                                                                                             >  2:9402195/1‑150 (MQ=255)
                                                     aGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGAcccc                                                                                            >  2:8973411/1‑150 (MQ=255)
                                                       gggTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCgg                                                                                          >  1:2102823/3‑150 (MQ=255)
                                                        tGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGc                                                                                         <  2:472825/150‑1 (MQ=255)
                                                        tGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGc                                                                                         >  2:10024095/1‑150 (MQ=255)
                                                           aaaaaGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCcgc                                                                                      >  2:910367/1‑150 (MQ=255)
                                                            aaaaGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCcgcg                                                                                     >  2:2670279/1‑150 (MQ=255)
                                                            aaaaGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCcgcg                                                                                     >  1:917961/1‑150 (MQ=255)
                                                               aGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGccc                                                                                  <  1:4725059/150‑1 (MQ=255)
                                                                 cTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCAACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAg                                                                                >  1:8901688/1‑150 (MQ=255)
                                                                     aaGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGcc                                                                            >  2:6654026/1‑150 (MQ=255)
                                                                       ggAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTc                                                                          >  2:10101300/1‑150 (MQ=255)
                                                                          aGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTcgcg                                                                       <  2:5018751/150‑1 (MQ=255)
                                                                            ttgttCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCt                                                                     >  1:8613807/1‑150 (MQ=255)
                                                                            gtgttCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCt                                                                     <  2:8675599/149‑1 (MQ=255)
                                                                              gttCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTcc                                                                   >  2:5426583/1‑150 (MQ=255)
                                                                               ttCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCa                                                                  <  1:10004843/150‑1 (MQ=255)
                                                                                tCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCATGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGTCTAGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAg                                                                 <  1:3290167/150‑1 (MQ=255)
                                                                                 cgccgcgccgCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGc                                                                <  1:4072496/150‑1 (MQ=255)
                                                                                   ccgcgccgcTCCACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGTGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTc                                                              <  2:7603750/150‑1 (MQ=255)
                                                                                       gccgcTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGccc                                                          <  1:10865799/150‑1 (MQ=255)
                                                                                        ccgcTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCa                                                         <  2:3792329/150‑1 (MQ=255)
                                                                                           cTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGc                                                      >  2:5602252/1‑150 (MQ=255)
                                                                                           cTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGc                                                      >  2:4179975/1‑150 (MQ=255)
                                                                                              aaCCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCgg                                                   >  2:2176844/1‑150 (MQ=255)
                                                                                              aaCCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCgg                                                   >  1:10732413/1‑150 (MQ=255)
                                                                                               aCCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGAGGGTCGACCCCGGACGCGCCCAGGGGCTCGCGCTCCAGCTCGCCCAGGCGGgc                                                  >  2:8320650/1‑150 (MQ=255)
                                                                                                  ggCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGcc                                               >  1:7759837/1‑150 (MQ=255)
                                                                                                      gCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAgc                                           >  1:6425456/1‑150 (MQ=255)
                                                                                                        tGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAgcgc                                         <  2:10235269/150‑1 (MQ=255)
                                                                                                        tGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAgcgc                                         >  1:1306549/1‑150 (MQ=255)
                                                                                                         gAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGcc                                        <  2:6469412/150‑1 (MQ=255)
                                                                                                          aGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGcca                                       <  1:7899552/150‑1 (MQ=255)
                                                                                                           gTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGccac                                      >  1:95048/1‑150 (MQ=255)
                                                                                                           gTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGccac                                      <  2:5254475/150‑1 (MQ=255)
                                                                                                            tCGGCTTCGCCGAGGACGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGACTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGccacc                                     <  2:799026/150‑1 (MQ=255)
                                                                                                            tCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGccacc                                     <  2:71597/150‑1 (MQ=255)
                                                                                                             cGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGccacca                                    <  1:6214511/150‑1 (MQ=255)
                                                                                                             cGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGccacca                                    >  2:6066297/1‑150 (MQ=255)
                                                                                                              ggCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAg                                   <  1:2847050/150‑1 (MQ=255)
                                                                                                                cTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGc                                 >  2:10156786/1‑150 (MQ=255)
                                                                                                                  tCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGccc                               >  2:1936546/1‑150 (MQ=255)
                                                                                                                     ccGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCCGGGCCTCGCGCGCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGCCCATg                            >  1:4350324/1‑150 (MQ=255)
                                                                                                                     ccGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGCCCATg                            <  2:6926069/150‑1 (MQ=255)
                                                                                                                      cGAGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGCCCATgg                           >  2:8167945/1‑150 (MQ=255)
                                                                                                                        aGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGCCCATGGAg                         <  1:3521402/150‑1 (MQ=255)
                                                                                                                        aGGAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGCCCATGGAg                         <  1:7144140/150‑1 (MQ=255)
                                                                                                                         ggAAGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGCCCATGGAGt                        <  1:6606689/150‑1 (MQ=255)
                                                                                                                           aaGCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGCCCATGGAGTgg                      <  1:9869166/150‑1 (MQ=255)
                                                                                                                             gCCGGCGCGGCGCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGCCCATGGAGTGGCa                    <  1:2506885/150‑1 (MQ=255)
                                                                                                                               cggcgcggcgCAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGCCCATGGAGTGGCAGc                  >  1:5313171/1‑150 (MQ=255)
                                                                                                                                ggcgcggcgcAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGCCCATGGAGTGGCAGCt                 >  1:8159366/1‑150 (MQ=255)
                                                                                                                                ggcgcggcgcAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGCCCATGGAGTGGCAGCt                 >  2:3620233/1‑150 (MQ=255)
                                                                                                                                ggcgcggcgcAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGCCCATGGAGTGGCAGCt                 <  1:8024125/150‑1 (MQ=255)
                                                                                                                                 gcgcggcgcAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGCCCATGGAGTGGCAGCtt                <  1:7555571/150‑1 (MQ=255)
                                                                                                                                   gcggcgcAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGCCCATGGAGTGGCAGCTTcc              >  1:3089826/1‑150 (MQ=255)
                                                                                                                                    cggcgcAGGGCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGCCCAGGGAGTGGCAGCTTCCg             >  2:28369/1‑150 (MQ=255)
                                                                                                                                           gggCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGCCCATGGAGTGGCAGCTTCCGGACAggc      >  1:2725455/1‑150 (MQ=255)
                                                                                                                                            ggCTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGCCCATGGAGTGGCAGCTTCCGGACAggcg     <  2:10944051/150‑1 (MQ=255)
                                                                                                                                              cTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGCCCATGGAGTGGCAGCTTCCGGACAggcgat   <  2:1403564/150‑1 (MQ=255)
                                                                                                                                              cTTCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGCCCATGGAGTGGCAGCTTCCGGACAggcgat   >  2:757384/1‑150 (MQ=255)
                                                                                                                                               ttCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGCCCATGGAGTGGCAGCTTCCGGACAggcgatg  <  2:1903361/150‑1 (MQ=255)
                                                                                                                                               ttCGGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGCCCATGGAGTGGCAGCTTCCGGACAggcgatg  >  1:2230499/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
GATGAGCGGATTATTGGCGGCGGATGTAAGACGCCGTTGAACGCGCGGATCGAAGATGTAAAAAGCTGTAAGGAAGTTGTTCGCCGCGCCGCTCAACCGGCCGCTGAGTCGGCTTCGCCGAGGAAGCCGGCGCGGCGCAGGGCTTCTGCGCCGTCGCGGCGCAGGCTACCCAGGCGTGCGCCCCAGGCTCGCGGGTCGACCCCGGCCGCGCCCAGGGCCTCGCGCTCCAGCTCGCCCAGGCCGGCGCCGAGCGCCACCAGGCCCATGGAGTGGCAGCTTCCGGACAGGCGATG  >  NC_008463/5866584‑5866876

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑