Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 6,076,066 G→C R105R (CGG→CGC)  PA14_RS27775 → methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084636,076,0660GC99.8% 2000.3 / NA 529R105R (CGG→CGC) PA14_RS27775methyltransferase
Reads supporting (aligned to +/- strand):  ref base G (1/0);  major base C (268/259);  minor base A (0/1);  total (269/260)
Fisher's exact test for biased strand distribution p-value = 4.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 529 total aligned reads displayed.

GCGCAGCCTCGAACAGGCCGACGCGGAGCACAACCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGGTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGGCCGCCTGCTGGCCCATGCGGGGCAACC  >  NC_008463/6075919‑6076212
                                                                                                                                                   |                                                                                                                                                  
 cgcAGCCTCGAACAGGCCGACGCGGAGCACAACCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTc                                                                                                                                                 >  2:7088327/1‑150 (MQ=255)
  gcAGCCTCGAACAGGCCGACGCGGAGCACAACCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCa                                                                                                                                                <  1:1299532/150‑1 (MQ=255)
   cAGCCTCGAACAGGCCGACGCGGAGCACAACCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCaa                                                                                                                                               >  2:1654681/1‑150 (MQ=255)
    aGCCTCGAACAGGCCGACGCGGAGCACAACCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAAt                                                                                                                                              >  1:3807720/1‑150 (MQ=255)
      ccTCGAACAGGCCGACGCGGAGCACAACCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGt                                                                                                                                            <  2:5279124/150‑1 (MQ=255)
      ccTCGAACAGGCCGACGCGGAGCACAACCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGt                                                                                                                                            <  1:3247796/150‑1 (MQ=255)
       cTCGAACAGGCCGACGCGGAGCACAACCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTc                                                                                                                                           <  1:5701526/150‑1 (MQ=255)
          gAACAGGCCGACGCGGAGCACAACCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGAc                                                                                                                                        <  2:9844388/150‑1 (MQ=255)
           aaCAGGCCGACGCGGAGCACAACCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGCACCGCTTCAATGTCGACg                                                                                                                                       >  1:10070634/1‑150 (MQ=255)
            aCAGGCCGACGCGGAGCACAACCAGCCCACCCGCCTGGCCGCGCCCGCCCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGt                                                                                                                                      >  1:572598/1‑150 (MQ=255)
               ggCCGACGCGGAGCACAACCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTAcc                                                                                                                                   >  2:4551339/1‑150 (MQ=255)
               ggCCGACGCGGAGCACAACCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTAcc                                                                                                                                   >  1:5840029/1‑150 (MQ=255)
                  cGACGCGGAGCACAACCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCggg                                                                                                                                <  1:3151546/150‑1 (MQ=255)
                   gACGCGGAGCACAACCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCAGTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGa                                                                                                                               >  2:6698015/1‑150 (MQ=255)
                      gcgGAGCACAACCAGCCCACCCGCCTGGCCGCGCCCGCGCCTGATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGc                                                                                                                            <  2:10028321/150‑1 (MQ=255)
                        ggAGCACAACCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCaa                                                                                                                          >  1:6550776/1‑150 (MQ=255)
                        ggAGCACAACCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCaa                                                                                                                          <  2:9515931/150‑1 (MQ=255)
                         gAGCACAACCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAg                                                                                                                         <  1:6710465/150‑1 (MQ=255)
                         gAGCACAACCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACAGGGACGCAAg                                                                                                                         <  1:1171060/150‑1 (MQ=255)
                            cacaACCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTgg                                                                                                                      <  1:8550330/150‑1 (MQ=255)
                             acaACCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCCGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCTAGTGgc                                                                                                                     >  2:8774518/1‑150 (MQ=255)
                               aaCCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGgcag                                                                                                                   >  1:9142376/1‑150 (MQ=255)
                                aCCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGCACCGCTTCAATGTCGACGGACCGGGACGCAAGTGgcagc                                                                                                                  <  2:3665447/150‑1 (MQ=255)
                                   aGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCCGGCCGCAGCGCTGGGCGCGGTCGGCGACCTGCCATGGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGa                                                                                                               <  2:4059122/150‑1 (MQ=255)
                                    gCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGAt                                                                                                              <  1:8909939/150‑1 (MQ=255)
                                    gCCCACCCGCCTGGCCGCGCCCGCGCCTTAGCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGAt                                                                                                              <  2:5680884/150‑1 (MQ=255)
                                     cccACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATc                                                                                                             <  2:10817732/150‑1 (MQ=255)
                                         cccGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGcc                                                                                                         >  1:9247722/1‑150 (MQ=255)
                                         cccGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGcc                                                                                                         >  1:2476949/1‑150 (MQ=255)
                                           cGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTg                                                                                                       <  2:8195320/150‑1 (MQ=255)
                                               tGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCg                                                                                                   >  1:8293535/1‑150 (MQ=255)
                                                ggCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGc                                                                                                  <  1:10865741/150‑1 (MQ=255)
                                                ggCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGc                                                                                                  >  1:4472270/1‑150 (MQ=255)
                                                     cgcccgcgccTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGgc                                                                                             <  2:4730757/150‑1 (MQ=255)
                                                              cTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGt                                                                                    >  2:5157335/1‑150 (MQ=255)
                                                               ttATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGtt                                                                                   <  2:86087/150‑1 (MQ=255)
                                                                tATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTc                                                                                  >  2:931192/1‑150 (MQ=255)
                                                                   ccGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGcc                                                                               <  2:8382665/150‑1 (MQ=255)
                                                                   ccGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGcc                                                                               <  2:4637869/150‑1 (MQ=255)
                                                                    cGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGccc                                                                              >  2:4999565/1‑150 (MQ=255)
                                                                     gCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCccg                                                                             <  2:1898577/150‑1 (MQ=255)
                                                                       aGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCccggc                                                                           >  2:1429577/1‑150 (MQ=255)
                                                                        gCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGgcag                                                                                                                   >  1:3058344/1‑109 (MQ=255)
                                                                        gCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACAGCTCCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCccggcc                                                                          <  2:2446482/150‑1 (MQ=255)
                                                                        gCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATGGGCGGACCGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCccggcc                                                                          <  2:6186322/150‑1 (MQ=255)
                                                                          tCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCccggccgg                                                                        >  1:1623667/1‑150 (MQ=255)
                                                                           cgccgcCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGACCCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTGCGCCCGGCCGgt                                                                       <  2:3107822/150‑1 (MQ=255)
                                                                           cgccgcCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGgt                                                                       <  2:127933/150‑1 (MQ=255)
                                                                           cgccgcCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCAGTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGgt                                                                       <  1:7660226/150‑1 (MQ=255)
                                                                           cgccgcCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGACATTGGCGGAACGCCCGCCGGCACCGAACCGCATCAATGTCGACGTAACGGGACGCAAGTGGCAGCAGATCGGACTGATCGCCGGGCGCCTGCAGTTCTCCCGGCCGgt                                                                       >  2:5413388/1‑150 (MQ=255)
                                                                             ccgccCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGgtcg                                                                     >  2:6926579/1‑150 (MQ=255)
                                                                               gccCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAAGGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGgtcgtc                                                                   <  2:2286361/150‑1 (MQ=255)
                                                                                cccAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCACGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTcc                                                                  >  1:1374425/1‑150 (MQ=255)
                                                                                  cAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCAc                                                                <  1:9396495/150‑1 (MQ=255)
                                                                                      ccGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGc                                                            <  1:6862156/150‑1 (MQ=255)
                                                                                      ccGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGACGTCCACAGGc                                                            >  1:4723552/1‑150 (MQ=255)
                                                                                       cGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCt                                                           <  1:9707796/150‑1 (MQ=255)
                                                                                          aGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGa                                                        >  1:6198441/1‑150 (MQ=255)
                                                                                             gcTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTg                                                     <  1:9575122/150‑1 (MQ=255)
                                                                                              cTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTgg                                                    >  1:7193414/1‑150 (MQ=255)
                                                                                                gggCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCTGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTg                                                  >  2:10286910/1‑150 (MQ=255)
                                                                                                gggCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTg                                                  <  2:488781/150‑1 (MQ=255)
                                                                                                gggCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTg                                                  >  2:10993852/1‑150 (MQ=255)
                                                                                                    gcgGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTcc                                              >  1:36871/1‑150 (MQ=255)
                                                                                                     cgGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGGTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCg                                             <  1:1033848/150‑1 (MQ=255)
                                                                                                      ggTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCgg                                            <  2:4304924/150‑1 (MQ=255)
                                                                                                         cGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCaa                                         >  1:9902767/1‑150 (MQ=255)
                                                                                                          ggCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCCGTTCGCCCGGCCGGTCGTCCACGGGCTCGTCTGGTGCTCCGGCAAg                                        >  2:10158895/1‑150 (MQ=255)
                                                                                                          ggCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAg                                        >  2:584237/1‑150 (MQ=255)
                                                                                                           gCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGGACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAgg                                       >  1:4057286/1‑150 (MQ=255)
                                                                                                           gCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAgg                                       >  2:7545829/1‑150 (MQ=255)
                                                                                                           gCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAgg                                       >  2:6403338/1‑150 (MQ=255)
                                                                                                           gCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAgg                                       >  1:4787219/1‑150 (MQ=255)
                                                                                                            cGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCATTGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAggg                                      >  1:503794/1‑150 (MQ=255)
                                                                                                            cGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAggg                                      >  2:6794242/1‑150 (MQ=255)
                                                                                                            cGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCAATGGCACGACTGGTGCTCCGGCAAggg                                      >  1:5313621/1‑150 (MQ=255)
                                                                                                              aaCTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGTTCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCCCTGGCGCGACTGGTGCTCCGGCAAGGGcc                                    >  2:3304390/1‑150 (MQ=255)
                                                                                                              aaCTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGcc                                    >  1:7436061/1‑150 (MQ=255)
                                                                                                               aCTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCa                                   >  2:2609024/1‑150 (MQ=255)
                                                                                                                cTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCAt                                  >  2:2880364/1‑150 (MQ=255)
                                                                                                                     aTTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTTCCGGGACGCAAGTTGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTGCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTggg                             <  2:3464764/150‑1 (MQ=255)
                                                                                                                     aTTGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTggg                             >  1:80609/1‑150 (MQ=255)
                                                                                                                      ttGGCGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGgc                            >  2:7138404/1‑150 (MQ=255)
                                                                                                                          cGGAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGgccgcc                        <  1:35415/150‑1 (MQ=255)
                                                                                                                           ggACCGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGGCCGCct                       <  1:10559638/150‑1 (MQ=255)
                                                                                                                            gAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGGCCGCctg                      >  1:5952388/1‑150 (MQ=255)
                                                                                                                            gAACGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGGCCGCctg                      >  1:10735048/1‑150 (MQ=255)
                                                                                                                             aaCGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGGCCGCctgc                     <  1:2244990/150‑1 (MQ=255)
                                                                                                                             aaCGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGGCCGCctgc                     <  1:8793034/150‑1 (MQ=255)
                                                                                                                              aCGCCCGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGGCCGCctgct                    >  1:6332405/1‑150 (MQ=255)
                                                                                                                                 cccGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGGCCGCCTGCTGGc                 <  2:1602074/150‑1 (MQ=255)
                                                                                                                                 cccGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGGCCGCCTGCTGGc                 >  1:2676956/1‑150 (MQ=255)
                                                                                                                                 cccGCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGGCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGGCCGCCTGCTGGc                 >  1:2899038/1‑150 (MQ=255)
                                                                                                                                    gCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGGCCGCCTGCTGGCCCa              <  1:5510842/150‑1 (MQ=255)
                                                                                                                                    gCAGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGCCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGGCCGCCTGCTGGccct              >  1:7904339/1‑149 (MQ=255)
                                                                                                                                      aGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGGCCGCCTGCTGGCCCATg            >  2:7802319/1‑150 (MQ=255)
                                                                                                                                      aGGCACCGAACCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCAGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGGCCGCCTGCTGGCCCATg            >  1:7520229/1‑150 (MQ=255)
                                                                                                                                               aCCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGGCCGCCTGCTGGCCCATGCGGGGCAAc   >  2:8085770/1‑150 (MQ=255)
                                                                                                                                               aCCGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGGCCGCCTGCTGGCCCATGCGGGGCAAc   <  1:2369124/150‑1 (MQ=255)
                                                                                                                                                ccGCTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGACCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGGCCGCCTGCTGGCCCATGCGGGGCAAcc  >  1:1765296/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GCGCAGCCTCGAACAGGCCGACGCGGAGCACAACCAGCCCACCCGCCTGGCCGCGCCCGCGCCTTATCCGCAGCTCGCCGCCCAGGCCGCAGCGCTGGGCGCGGTCGGCGAACTGCCATTGGCGGAACGCCCGCAGGCACCGAACCGGTTCAATGTCGACGTACCGGGACGCAAGTGGCAGCAGATCGGCCTGTTCGCCGGGCGCCTGCAGTTCGCCCGGCCGGTCGTCCACTGGCTCGACTGGTGCTCCGGCAAGGGCCATCTGGGCCGCCTGCTGGCCCATGCGGGGCAACC  >  NC_008463/6075919‑6076212

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑