Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 966,217 T→C intergenic (+77/‑568) PA14_RS04485 → / → PA14_RS04490 fimbrial protein/response regulator transcription factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_008463966,2170TC100.0% 1520.1 / NA 393intergenic (+77/‑568)PA14_RS04485/PA14_RS04490fimbrial protein/response regulator transcription factor
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (204/189);  total (204/189)

Only 100 of 393 total aligned reads displayed.

TCGGCGCGGAGGGCGAATTGAAGGCCGGGGTCGCCGACGGCGCCGCCGAGTTCACGTTTACCTTCCCCTGAGCGGCGCCGCCGCTCTTTCCCCGGCGTTTCCCGCCCCGCCTCGGGAAGCGCCGTCGGCCGCTTTCGCGCCAGCCCTTGCGAAGGCTCGGCCCCTCACCTCCAGTGGTTGCAGATCGCCGAAGGCGCTCTGCATATCGCTCCGCTGCTCGTCCTGGGTCTCGTGCGCACATGCCATTCCCGCACGCGGAATACCGATATTGCGTATGGGCGAAGGCTTTCTGAG  >  NC_008463/966070‑966363
                                                                                                                                                   |                                                                                                                                                  
   gcgcGGAGGGCGAATTGAAGGCCGGGGTCGCCGACGGCGCCGCCGAGTTCACGTTTACCTTCCCCTGAGCGGCGCCGCCGCTCTTTCCCCGGCGTTTCCCGCCCCGCCTCGGGAAGCGCCGTCGGCCGCTTTCGCGCCAGCCCTCGCGaa                                                                                                                                               <  1:2918365/150‑1 (MQ=255)
     gcgGAGGGCGAATTGATGGCCGGGGTCGCCGACGGCGCCGCCGAGTTCACGTTTACCTTCCCCTGTGCGGCGCCGCCGCTCTTTCCCCGGCGTTTCCCGCCCCGCCTCGGGAAGCGCCGTCGGCCGCTTTCGCGCCAGCCCTCGCGAAgg                                                                                                                                             <  2:930547/150‑1 (MQ=255)
     gcgGAGGGCGAATTGAAGGCCGGGGTCGCCGACGGCGCCGCCGAGTTCACGTTTACCTTCCCCTGAGCGGCGCCGCCGCTCTTTCCCCGGCGTTTCCCGCCCCGCCTCGGGAAGCGCCGTCGGCCGCTTTCGCGCCAGCCCTCGCGAAgg                                                                                                                                             >  1:1991607/1‑150 (MQ=255)
      cgGAGGGCGAATTGAAGGCCGGGGTCGCCGACGGCGCCGCCGAGTTCACGTTTACCTTCCCCTGAGCGGCGCCGCCGCTCTTTCCCCGGCGTTTCCCGCCCCGCCTCGGGAAGCGCCGTCGGCCGCTTTCGCGCCAGCCCTCGCGAAGGc                                                                                                                                            >  2:3718912/1‑150 (MQ=255)
       ggAGGGCGAATTGAAGGCCGGGGTCGCCGACGGCGCCGCCGAGTTGACGTTTACCTTCCCCTGAGCGGCGCCGCCGCTCTTTCCCCGGCGTTTCCCGCCCCGCCTCGGGAAGCGCCGTCGGCCGCTTTCGCGCCAGCCCTCGCGATGGcg                                                                                                                                           >  1:9582691/1‑149 (MQ=255)
       ggAGGGCGAATTGAAGGCCGGGGTCGCCGACGGCGCCGCCGAGTTCACGTTTACCTTCCCCTGAGCGGCGCCGCCGCTCTTTCCCCGGCGTTTCCCGCCCCGCCTCGGGAAGCGCCGTCGGCCGCTTTCGCGCCAGCCCTCGCGAAGGCt                                                                                                                                           >  1:7235905/1‑150 (MQ=255)
       ggAGGGCGAATTGAAGGCCGGGGTCGCCGACGGCGCCGCCGAGTTCACGTTTACCTTCCCCTGAGCGGCGCCGCCGCTCTTTCCCCGGCGTTTCCCGCCCCGCCTCGGGAAGCGCCGTCGGCCGCGTTCGCGCCAGCCCTCGCGAAGGCt                                                                                                                                           >  1:5064209/1‑150 (MQ=255)
         aGGGCGAATTGAAGGCCGGGGTCGCCGACGGCGCCGCCGAGTTCACGTTTACCTTCCCCTGAGCGGCGCCGCCGCTCTTTCCCCGGCGTTTCCCGCCCCGCCTCGGGAAGCGCCGTCGGCCGCTTTCGCGCCAGCCCTCGCGAAGGCTCg                                                                                                                                         >  1:8090937/1‑150 (MQ=255)
          gggCGAATTGAAGGCCGGGGTCGCCGACGGCGCCGCCGAGTTCACGTTTACCTTCCCCTGAGCGGCGCCGCCGCTCTTTCCCCGGCGTTTCCCGCCCCGCCTCGGGAAGCGCCGTCGGCCGCTTTCGCGCCAGCCCTCGCGAAGGCTCgg                                                                                                                                        >  2:3625146/1‑150 (MQ=255)
          gggCGAATTGAAGGCCGGGGTCGCCGACGGCGCCGCCGAGTTCACGTTTACCTTCCCCTGAGCGGCGCCGCCGCTCTTTCCCCGGCGTTTCCCGCCCCGCCTCGGGAAGCGCCGTCGGCCGCTTTCGCGCCAGCCCTCGCGAAGGCTCgg                                                                                                                                        <  1:5898531/150‑1 (MQ=255)
               acttGAAGGCCGGGGTCGCCGACGGCGCCGCCGAGTTCACGTTTACCTTCCCCTGAGCGGCGCCGCCGCTCTTTCCCCGGCGTTTCCCGCCCCGCCTCGGGAAGCGCCGTCGGCCGCGTTCGCGCCAGCCCTCGCGAAGGCTCGGCCCCt                                                                                                                                   >  1:863086/3‑150 (MQ=255)
               aaTTGAAGGCCGGGGTCGCCGACGGCGCCGCCGAGTTCACGTTTACCTTCCCCTGAGCGGCGCCGCCGCTCTTTCCCCGGCGTTTCCCGCCCCGCCACGGGAAGCGCCGTCGGCCGCTTTCGCGCCAGCCCTCGCGAAGGCTCGGCCCCt                                                                                                                                   >  1:1107803/1‑150 (MQ=255)
                   gAAGGCCGGGGTCGCCGACGGCGCCGCCGAGTTCACGTTTACCTTCCCCTGAGCGGCGCCGCCGCTCTTTCCCCGGCGTTTCCCGCCCCGCCGCGGGAAGCGCCGTCGGCCGCTTTCGCGCCAGCCCTCGCGAAGGCTCGGCCCCTCAcc                                                                                                                               >  2:4862328/1‑150 (MQ=255)
                    aaGGCCGGGGTCGCCGACGGCGCCGCCGAGTTCACGTTTACCTTCCCCTGAGCGGCGCCGCCGCTCTTTCCCCGGCGTTTCCCGCCCCGCCTCGGGAAGCGCCGTCGGCCGCTTTCGCGCCAGCCCTCGCGAAGGCTCGGCCCCTCACCt                                                                                                                              <  2:6115196/150‑1 (MQ=255)
                     aGGCCGGGGTCGCCGACGGCGCCGCCGAGTTCACGTTTACCTTCCCCTGAGCGGCGCCGCCGCTCTTTCCCCGGCGTTTCCCGCCCCGCCTCGGGAAGCGCCGTCGGCCGCTTTCGCGCCAGCCCTCGCGAAGGCTCGGCCCCTCACCTc                                                                                                                             <  2:5983135/150‑1 (MQ=255)
                        ccGGGGTCGCCGACGGCGCCGCCGAGTTCACGATTACCTTCCCCTGAGCGGCGCCGCCGCTCTTTCCCCGGCGTTTCCCGCCCCGCCTCGGGAAGCGCCGTCGGCCGCTTTCGCGCCAGCCCTCGCGAAGGCTCGGCCCCTCACCTCCAg                                                                                                                          <  1:618698/150‑1 (MQ=255)
                          ggggTCGCCGACGGCGCCGCCGAGTTCACGTTTACCTTCCCCTGAGCGGCGCCGCCGCTCTTTCCCCGGCGTTTCCCGCCCCGCCTCGGGAAGCGCCGTCGGCCGCTTTCGCGCCAGCCCTCGCGAAGGCTCGGCCCCTCACCTCCAGTg                                                                                                                        >  2:8809847/1‑150 (MQ=255)
                          ggggTCGCCGACGGCGCCGCCGAGTTCACGTTTACCTTCCCCTGAGCGGCGCCGCCGCTCTTTCCCCGGCGTTTCCCGCCCCGCCTCGGGAAGCGCCGTCGGCCGCTTTCGCGCCAGCCCGCGCGAAGGCTCGGCCCCTCACCTCCAggg                                                                                                                        >  1:4852925/1‑148 (MQ=255)
                            ggTCGCCGACGGCGCCGCCGAGTTCACGTTTACCTTCCCCTGAGCGGCGCCGCCGCTCTTTCCCCGGCGTTTCCCGCCCCGCCTCGGGAAGCGCCGTCGGCCGCTTTCGCGCCAGCCCTCGCGAAGGCTCGGCCCCTCACCTCCAGTGGt                                                                                                                      <  1:10777928/150‑1 (MQ=255)
                             gTCGCCGACGGCGCCGCCGAGTTCACGTTTACCTTCCCCTGAGCGGCGCCGCCGCTCTTTCCCCGGCGTTTCCCGCCCCGCCTCGGGAAGCGCCGTCGGCCGCTTTCGCGCCAGCCCTCGCGAAGGCTCGGCCCCTCACCTCCAGTGGtt                                                                                                                     >  1:10012987/1‑150 (MQ=255)
                             gTCGCCGACGGCGCCGCCGAGTTCACGTTTACCTTACCCTGAGCGGCGCCGCCGCTCTTTCCACGGCGTGTCCCGCCCCGCCTCGGGAAGCGCCGTCGGCCGCTTTCGCGCCAGCCCTCGCGAAGGCTCGGCCCCTCACCTCCAGTGGtt                                                                                                                     <  2:793666/150‑1 (MQ=255)
                                 ccGACGGCGCCGCCGAGTTCACGTTTACCTTCCCCTGAGCGGCGCCGCCGCTCTTTCCCCGGCGTTTCCCGCCCCGCCTCGGGAAGCGCCGTCGGCCGCTTTCGCGCCAGCCCTCGCGAAGGCTCGGCCCCTCACCTCCAGTGGTTGCAg                                                                                                                 >  2:3030429/1‑150 (MQ=255)
                                   gACGGCGCCGCCGAGTTCACGTTTACCTTGCCGTGTGCGGCGGCGCCGCTCTTTCCCCGGCGTTTCCCGCCCCGCCTCGGGAAGCGCCGTCGGCCGCTGTCGCGCCCGCCCGCGCGTAGGCTCGGCCCCTCTCCTCCAGTGGGTGGAgtt                                                                                                               >  2:4318976/1‑148 (MQ=255)
                                   gACGGCGCCGCCGAGTTCACGTTTACCTTCCCCTGAGCGGCGCCGCCGCTCTTTCCCCGGCGTTTCCCGCCCCGCCTCGGGAAGCGCCGTCGGCCGCTTTCGCGCCAGCCCTCGCGAAGGCTCGGCCCCTCACCTCCAGTGGTTGGAGAt                                                                                                               >  1:5243346/1‑150 (MQ=255)
                                    aCGGCGCCGCCGAGATCACGTTTACCTTCCCCTGAGCGGCGCCGCCGCTCGAACCCCGGCGTTTCCCGCACCGCCTCGGGACGCGCCGTCGGCCGCTGTCGCTCCAGCCCTCGCGAAGGCTCGTCCCCTCACCTCCAGTGGTAGCAGATc                                                                                                              >  2:56664/1‑150 (MQ=255)
                                     cGGCGCCGCCGAGTTCACGTTTACCTTCCCCTGAGCGGCGCCGCCGCTCTTTCCCCGGCGTTTCCCGCCCCGCCTCGGGAAGCGCCGTCGGCCGCTTTCGCGCCAGCCCTCGCGAAGGCTCGGCCCCTCACCTCCAGTGGTTGCAGATCg                                                                                                             <  1:6355850/150‑1 (MQ=255)
                                      ggCGCCGCCGAGTTCACGTTTACCTTCCCCTGAGCGGCGCCGCCGCTCTTTCCCCGGCGTTTCCCGCCCCGCCTCGGGAAGCGCCGTCGGCCGCTTTCGCGCCAGCCCTCGCGAAGGCTCGGCCCCTCACCTCCAGTGGTTGCAGATCGc                                                                                                            <  1:7670629/150‑1 (MQ=255)
                                      ggCGCCGCCGAGTTCACGTTTACCTTCCCCTGAGCGGCGCCGCCGCTCTTTCCCCGGCGTTTCCCGCCCCGCCTCGGGAAGCGCCGTCGGCCGCTTTCGCGCCAGCCCTCGCGAAGGCTCGGCCCCTCACCTCCAGTGGTGTCAGaaagc                                                                                                            >  1:5558007/1‑146 (MQ=255)
                                      ggCGCCGCCGAGTTCACGTTTACCTTCCCCTGAGCGGCGCCGCCGCTCTTTCCCCGGCGTTTCCCGCCCCGCCTCGGGAAGCGCCGTCGGCCGCTGTCGCGCCAGCCCTCGCGAAGGCTCGGCCCCTCACCTCCAGTGGTTGCAGATCGc                                                                                                            >  2:1581267/1‑150 (MQ=255)
                                       gcgcCGCCGAGTTCACGTTTACCTTCCCCTGAGCGGCGCCGCCGCTCTTTCCCCGGCGTTTCCCGCCCCGCCTCGGGAAGCGCCGTCGGCCGCTTTCGCGCCAGCCCTCGCGAAGGCTCGGCCCCTCACCTCCAGTGGTTGgagaaagcc                                                                                                           >  1:33605/1‑145 (MQ=255)
                                         gcctccGAGTTCACGTTTACCATCCCCTGAGCGGCGCCGCCGCTCTTACCCCGGCGTTACCCGCCCCTCCTCGGGAAGCGCCGACGGCCCCTATCGCGCCAGCCCTCGCGAAGGCTCGGCCCCTCACCACCAGAGGTTTCAGATAGccca                                                                                                         >  1:9174700/1‑148 (MQ=255)
                                          ccgccgAGTTCACGTTTACCTTCCCCTGAGCGGCGCCGCCGCTCTTTCCCCGGCGTTTCCCGCCCCGCCTCGGGAAGCGCCGTCGGCCGCTTTCGCGCCAGCCCTCGCGAAGGCTCGGCCCCTCACCTCCAGTGGTTGCAGATCGCCGaa                                                                                                        <  1:6681126/150‑1 (MQ=255)
                                          ccgccgAGTTCACGTTTACCTTCCCCTGAGCGGCGCCGCCGCTCTTTCCCCGGCGTTTCCCGCCCCGCCTCGGGAAGCGCCGTCGGCCGCTTTCGCGCCAGCCCTCGCGAAGGCTCGGCCCCTCACCTCCAGTGGTTGCAGATCGCCGaa                                                                                                        >  2:101806/1‑150 (MQ=255)
                                               gAGTGCACGTTTACCTGCCCCTGAGCGGCGCCGCCGCTCTGTCCCCGGCGTTTCCCGCCCCGCCTCGGGAAGCGCCGTCGGCCGCTTTCGCGCCAGCCCTCGCGAAGGCTCGGCCCCTCACCTCCAGTGGTTGCAGATCGCCGAAGgcgc                                                                                                   <  2:5839798/150‑1 (MQ=255)
                                                aGTTCACGTTTACCTTCCCCTGAGCGGCGCCGCCGCTCTTTCCCCGGCGTTTCCCGCCCCGCCTCGGGAAGCGCCGTCGGCCGCTTTCGCGCCAGCCCTCGCGAAGGCTCGGCCCCTCACCTCCAGTGGTTGCAGATAGCCGAAGgcgcg                                                                                                  >  1:4052234/1‑149 (MQ=255)
                                                aGTTCACGTTTACCTTCCCCTGAGCGGCGCCGCCGCTCTTTCCCCGGCGTTTCCCGCCCCGCCTCGGGAAGCGCCGTCGGCCGCTTTCGCGCCAGCCCTCGCGAAGGCTCGGCCCCTCACCTCCAGTGGTTGCAGATAGCCGAAGGCGct                                                                                                  >  2:4962490/1‑150 (MQ=255)
                                                aGTTCACGTTTACCTTCCCCTGAGCGGCGCCGCCGCTCTTTCCCCGGCGTTTCCCGCCCCGCCTCGGGAAGCGCCGTCGGCCGCTTTCGCGCCAGCCCTCGCGAAGGCTCGGCCCCTCACCTCCAGTGGTTGCAGAAAGCCGAAGGCGct                                                                                                  >  2:10035246/1‑150 (MQ=255)
                                                 gTTCACGTTTACCTTCCCCTGAGCGGCGCCGCCGCTCTTTCCCCGGCGTTTCCCGCCCCGCCTCGGGAAGCGCCGTCGGCCGCTTTCGCGCCAGCCCTCGCGAAGGCTCGGCCCCTCACCTCCAGTGGTTGCAGATCGCCGAAGGCGctc                                                                                                 <  2:2289783/150‑1 (MQ=255)
                                                 gTTCACGTTTACCTTCCCCTGAGCGGCGCCGCCGCTCTTTCCCCGGCGTTTCCCGCCCCGCCTCGGGAAGCGCCGTCGGCCGCTTTCGCGCCAGCCCTCGCGAAGGCTCGGCCCCTCACCTCCAGTGGTTGCAGATCGCCGAAGGCGctc                                                                                                 >  2:6372728/1‑150 (MQ=255)
                                                  ttCACGTGTACCGTCCCCTGAGCGGCGCCGCCGCACTTTCCCCGGCGTTTCCCGCCCCGCCTCGGGAAGCGCCGTCGGCCGCTTTCGCGCCAGCCCTCGCGAAGGCTCGGCCCCTCACCTCCAGTGGTTGCAGATCGCCGAAGGCGctct                                                                                                <  2:7021233/150‑1 (MQ=255)
                                                  gtCACGTTTACCTTCCCCTGAGCGGCGCCGCCGCTCTTTCCCCGGCGTTTCCCGCCCCGCCTCGGGAAGCGCCGTCGGCCGCGTTCGCGCCAGCCCTCGCGAAGGCTCGGCCCCTCACCTCCAGTGGTTGCAGATCGCCGAAGGCGctct                                                                                                <  1:6525072/149‑1 (MQ=255)
                                                     ccGTTTACCTTCCCCTGAGCGGCGCCGCCGCTCTTTCCCCGGCGTTTCCCGCCCCGCCTCGGGAAGCGCCGTCGGCCGCTTTCGCGCCAGCCCTCGCGAAGGCTCGGCCCCTCACCTCCAGTGGTTGCAGATCGCCGAAGGCGCTCTGCa                                                                                             <  1:4694891/149‑1 (MQ=255)
                                                      cGTTTACCTTCCCCTGAGCGGCGCCGCCGCTCTTTCCCCGGCGTTTCCCGCCCCGACTCGGGAAGCGCCGTCGGCCGCTTTCGCGCCAGCCCTCGCGAAGGCTCGGCCCCTCACCTCCAGTGGTTGCAGATCGCCGAAGGCGCTCTGCat                                                                                            <  1:796226/150‑1 (MQ=255)
                                                        tttACCTTCCCCTGAGCGGCGCCGCCGCTCTTTCCCCGGCGTTTCCCGCCCCGCCTCGGGAAGCGCCGTCGGCCGCTTTCGCGCCAGCCCTCGCGAAGGCTCGGCCCCTCACCTCCAGTGGTTGCAGATCGCCGAAGGCGCTCTGCatat                                                                                          >  2:5768579/1‑150 (MQ=255)
                                                          tACCTTCCCCTGAGCGGCGCCGCCGCTCTTTCCCCGGCGTTTCCCGCCCCGCCTCGGGAAGCGCCGTCGGCCGCTTGCGCGCCAGCCCTCGCGAAGGCTCGGCCCCTCACCTCCAGTGGTTGCAGATCGCCGAAGGCGCTCTGCATATCg                                                                                        >  2:10945540/1‑150 (MQ=255)
                                                             cTTCCCCTGAGCGGCGCCGCCGCTCTTTCCCCGGCGTTTCCCGCCCCGCCTCGGGAAGCGCCGTCGGCCGCTTTCGCGCCAGCCCTCGCGAAGGCTCGGCCCCTCACCTCCCGTGGTTGCAGATCGCCGAAGGCGCTCTGCATATCGCTc                                                                                     <  1:156104/150‑1 (MQ=255)
                                                              ttCCCCTGAGCGGCGCCGCCGCTCTTTCCCCGGCGTTTCCCGCCCCGCCTCGGGAAGCGCCGTCGGCCGCTTTCGCGCCAGCCCTCGCGAAGGCTCGGCCCCTCACCTCCAGTGGTTGCAGATCGCCGAAGGCGCTCTGCATATCGCTcc                                                                                    <  2:7514569/150‑1 (MQ=255)
                                                                   cTGAGCGGCGCCGCCGCTCTTTCCCCGGCGTTTCCCGCCCCGCCTCGGGAAGCGCCGTCGGCCGCTTTCGCGCCAGCCCTCGCGAAGGCTCGGCCCCTCACCTCCAGTGGTTGCAGATCGCCGAAGGCGCTCAGCATATAGCTCCgcagc                                                                               >  2:4098992/1‑150 (MQ=255)
                                                                    tGAGCGGCGCCGCCGCTCTTTCCCCGGCGTTTCCCGCCCCGCCTCGGGAAGCGCCGTCGGCCGCTTTCGCGCCAGCCCTCGCGAAGGCTCGGCCCCTCACCTCCAGTGGTTGCAGATCGCCGAAGGCGCTCTGCATATCGCTCCgctgct                                                                              <  1:2493698/150‑1 (MQ=255)
                                                                    tGAGCGGCGCCGCCGCTCTTTCCCCGGCGTTTCCCGCCCCGCCTCGGGAAGCGCCGTCGGCCGCTTTCGCGCCAGCCCTCGCGAAGGCTCGGCCCCTCACCTCCAGTGGTTGCAGATCGCCGAAGGCGCTCTGCATATCGCTCCgctgct                                                                              >  2:10497501/1‑150 (MQ=255)
                                                                             ccgccgcTCTTTCCCCGGCGTTTCCCGCCCCGCCTCGGGAAGCGCCGTCGGCCGCTTTCGCGCCAGCCCTCGCGAAGGCTCGGCCCCTCACCTCCAGTGGTGGCAGATAGCCGAAGGCGCGCAGCATATAGCTCCGCGGCTCGTGCgggg                                                                     >  1:3132461/1‑150 (MQ=255)
                                                                              cgccgcTCTTTCCCCGGCGTTTCCCGCCCCGCCTCGGGAAGCGCCGTCGGCCGCTTTCGCGCCAGCCCTCGCGAAGGCTCGGCCCCTCACCTCCAGTGGTTGCAGATCGCCGAAGGCGCTCTGCATATCGCTCCGCTGCTCGTCCTGGGt                                                                    <  2:10104214/150‑1 (MQ=255)
                                                                               gccgcTCTTTCCCCGGCGTTTCCCGCCCCGCCTCGGGAAGCGCCGTCGGCCGCTTTCGCGCCAGCCCTCGCGAAGGCTCGGCCCCTCACCTCCAGTGGTTGCCGATCGCCGGAGGCGCGCTGCATATCGCGCCGCTGCTCGTCCTGGGtc                                                                   >  2:4449637/1‑150 (MQ=255)
                                                                               gccgcTCTTTCCCCGGCGTTTCCCGCCCCGCCTCGGGAAGCGCCGTCGGCCGCTTTCGCGCCAGCCCTCGCGAAGGCTCGGCCCCTCACCTCCAGTGGTTGCAGATCGCCGAAGGCGCTCTGCATATCGCTCCGCTGCTCGTCCTGGGtc                                                                   <  2:2028686/150‑1 (MQ=255)
                                                                                ccgcTCTTTCCCCGGCGTTTCCCGCCCCGCCTCGGGAAGCGCCGTCGGCCGCTTTCGCGCCAGCCCTCGCGAAGGCTCGGCCCCTCACCTCCAGTGGTTGCAGATCGCCGAAGGCGCTCTGCATATCGCTCCGCTGCTCGTCCTGGGtct                                                                  <  1:10584563/150‑1 (MQ=255)
                                                                                  gcTCTTTCCCCGGCGTTTCCCGCCCCGCCTCGGGAAGCGCCGTCGGCCGCTTTCGCGCCAGCCCTCGCGAAGGCTCGGCCCCTCACCTCCAGTGGTTTCAGATCGCCGAAGGCGCGATGCATATAGCTCCGCAGCGCGTCCTGGGTGTCg                                                                >  1:1469853/1‑150 (MQ=255)
                                                                                   ctctTTCCCCGGCGTTTCCCGCCCCGCCTCGGGAAGCGCCGTCGGCCGCTTTCGCGCCAGCCCTCGCGAAGGCTCGGCCCCTCACCTCCAGTGGTTGCAGATCGCCCGAGGCGCGCAGCCTATCGCTCCGCGGCGCGTCCTGGGTcgcgg                                                               >  1:8571039/1‑149 (MQ=255)
                                                                                     ctTTCCCCGGCGTTTCCCGCCCCGCCTCGGGACGCGCCGTCGGCCGCTTTCGCGCCAGCCCTCGCGAAGGCTCGGCCCCTCACCTCCAGTGGTTGCAGATCGCCGAAGGCGCTCTGCATCTCGCTCCGCTGCTCGTCCTGGGGCTCGTgc                                                             >  2:8982553/1‑150 (MQ=255)
                                                                                     ctTTCCCCGGCGTTTCCCGCCCCGCCTCGGGAAGCGCCGTCGGCCGCTTTCGCGCCAGCCCTCGCGAAGGCTCGGCCCCTCACCTCCAGTGGTTGCAGATCGCCGAAGGCGCGCTGCAGATGGCGCCGCTGCTCGGCCGGGGTCGCGTgc                                                             >  1:7900969/1‑150 (MQ=255)
                                                                                      tttCCCCGGCGTTTCCCGCCCCGCCTCGGGAAGCGCCGTCGGCCGCTTTCGCGCCAGCCCTCGCGAAGGCTCGGCCCCTCACCTCCAGTGGTTGCAGATCGCCGAAGGCGCTCTGCATATCGCTCCGCTGCTCGTCCTGGGTCTCGTgcg                                                            <  1:2985955/150‑1 (MQ=255)
                                                                                      tttCCCCGGCGTTTCCCGCCCCGCCTCGGGAAGCGCCGTCGGCCGCTTTCGCGCCAGCCCTCGCGAAGGCTCGGCCCCTCACCTCCAGTGGTTGCAGATCGCCGAAGGCCCTCGGCATATCGCTCCGCGGCACGTCCGGGGTCTCGTggg                                                            >  2:9964934/1‑148 (MQ=255)
                                                                                        tCCCCGGCGTTTCCCGCCCCGCCTCGGGAAGCGCCGTCGGCCGCTTTCGCGCCAGCCCTCGCGAAGGCTCGGCCCCTCACCTCCAGTGGTTGCAGATCGCCGAAGGCGCTCTGCATATCGCTCCGCTGCTCGTCCTGGGTCTCGTGCGca                                                          <  1:3848849/150‑1 (MQ=255)
                                                                                         ccccGGCGTTTCCCGCCCCGCCTCGGGAAGCGCCGTCGGCCGCTGTCGCGCCAGCCCTCGCGAAGGCTCGGCCCCTCACCTCCAGTGGTTGCAGATCGCCGAAGGCGCTCTGCATATCGCTCCGCTGCTCGTCCTGGGTCTCGTGCGcac                                                         <  2:2607684/150‑1 (MQ=255)
                                                                                          cccGGCGTTTCCCGCCCCGCCTCGGGAAGCGCCGTCGGCCGCTTTCGCGCCAGCCCTCGCGTAGGCTCGGCCCCTCACCTGCAGTGGTTGCAGATCGGCGTAGGCGCTCGGCAGCTCGGTCCGCGGCTCGTCCGGGGGCTCGTgcggcca                                                        >  2:7155112/1‑146 (MQ=255)
                                                                                           ccGGCGTTTCCCGCCCCGCCTCGGGAAGCGCCGTCGGCCGCTTTCGCGCCAGCCCTCGCGAAGGCTCGGCCCCTCACCTCCAGGGGGTGCAGATCGCCGAAGGCGCTCTGCATATCGCTCCGCTGCTCGTCCGGGGTCTCGTGCGCACAt                                                       >  2:8178118/1‑150 (MQ=255)
                                                                                            cGGCGTTTCCCGCCCCGCCTCGGGAAGCGCCGTCGGCCGCTTTCGCGCCAGCCCTCGCGAAGGCTCGGCCCCTCACCTCCAGTGGTTGCAGATCGCCGAAGGCGCTCTGCATATCGCTCCGCTGCTCGTGCTGGGTCTCGTGCGCACATg                                                      >  2:8414986/1‑150 (MQ=255)
                                                                                            cGGCGTTTCCCGCCCCGCCTCGGGAAGCGCCGTCGGCCGCTTTCGCGCCAGCCCTCGCGAAGGCTCGGCCCCTCACCTCCAGTGGTTGCAGATCGCCGAAGGCGCTCTGCATATCGCTCCGCTGCTCGTCCTGGGTCTCGTGCGCACATg                                                      <  2:6477277/150‑1 (MQ=255)
                                                                                                gTTTCCCGCCCCGCCTCGGGAAGCGCCGTCGGCCGCTTTCGCGCCAGCCCTCGCGAAGGCTCGGCCCCTCACCTCCAGTGGTTGCAGATCGCCGAAGGCGCTCTGCATCTCGCTCCGCTGCTCGTCCTGGGTCTCGTGCGCACATGCCAt                                                  >  2:10734246/1‑150 (MQ=255)
                                                                                                ctttCCCGCCCCGCCTCGGGAAGCGCCGTCGGCCGCTTTCGCGACAGCCCTCGCGAAGGCTCGGCCCCTCTCCTCCAGTGGTTGCAGATCGCCGAAGGCGCTCTGCATCTCGCTCCGCTGCTCGTCCTGGGTCTCGTGCGCACATGCCAt                                                  <  2:11056389/149‑1 (MQ=255)
                                                                                                       gccccgccTCGGGAAGCGCCGTCGGCCGCTTTCGCGCCAGCCCTCGCGAAGGCTCGGCCCCTCACCTCCAGTGGTTGCAGATCGCCGAAGGCGCTCTGCATATCGCTCCGCTGCTCGTCCTGGGTCTCGTGCGCACATGCCATTCCCGCa                                           <  2:6752179/150‑1 (MQ=255)
                                                                                                       gccccgccTCGGGAAGCGCCGTCGGCCGCTTTCGCGCCAGCCCTCGCGAAGGCTCGGCCCCTCACCTCCAGTGGTTGCAGATCGCCGAAGGCGCTCTGCATATCGCTCCGCTGCGCGTCCTGGGTCTCGTGCGCACAAGCCAATACCGCa                                           >  1:9028483/1‑150 (MQ=255)
                                                                                                        ccccgccTCGGGAAGCGCCGTCGGCCGCTTTCGCGCCAGCCCTCGCGAAGGCTCGGCCCCTCACCTCCAGTGGTTGCAGATCGCCGAAGGCGCGCTGCATATCGCTCCGCGGCGCGTCCGGGGGCTCGGGCGCACATGCCCTTCCCGCAc                                          >  2:3997223/1‑150 (MQ=255)
                                                                                                         cccgccTCGGGAAGCGCCGTCGGCCGCTTTCGCGCCAGCCCTCGCGAAGGCTCGGCCCCTCACCTCCAGTGGTTGCCGATCGCCCAAGGCGCGCTGCAAATCGCTCCGCAGCTCGGGCTGGGTGTCGTGCGCACAAGCCATTCCCGCAcg                                         >  2:12378/1‑150 (MQ=255)
                                                                                                            gccTCGGGAAGCGCCGTCGGCCGCTTTCGCGCCAGCCCTCGCGAAGGCTCGGCCCCTCACCTCCAGTGGTTGCAGATCGCCGAAGGCGCTCTGCATATCGCTCCGCTGCTCGTCCTGGGTCTCGTGCGCACATGCCATTCCCGCACGCgg                                      >  2:856672/1‑150 (MQ=255)
                                                                                                             ccTCGGGAAGCGCCGTCGGCCGCTTTCGCGCCAGCCCTCGCGAAGGCTCGGCCCCTCACCTCCAGTGGTTGCAGATCGCCGAAGGCGCTCTGCATATCGCTCCGCTGCTCGTCCTGGGTCTCGTGCGCACATGCCATTCCCGCACGCGGa                                     <  1:6935966/150‑1 (MQ=255)
                                                                                                             ccTCGGGAAGCGCCGTCGGCCGCTTTCGCGCCAGCCCTCGCGAAGGCTCGGCCCCTCACCTCCAGTGGTTGCAGATCGCCGAAGGCGCGCTGCATATAGCGCCGCGGCTCGGGCGGGGTCTCGGGGGCACATGCCATTCCCGCACGCggg                                     >  1:5187601/1‑149 (MQ=255)
                                                                                                              cTCGGGAAGCGCCGTCGGCCGCTTTCGCGCCAGCCCTCGCGAAGGCTCGGCCCCTCACCTCCCGGGGTTGCAGATCGCCGAAGGCGCGCAGCATATAGCTCCGCTGCGGGTCCGGGGTCGCGTGCGCACATGCCCATCCCGCCAGCGGaa                                    >  2:985634/1‑150 (MQ=255)
                                                                                                                  ggAAGCGCCGTCGGCCGCTTTCGCGCCAGCCCTCGCGAAGGCTCGGCCCCTCACCTCCAGTGGTTGCAGATCGCCGAAGGCGGTCTGCATATAGCTCCGCTGCGCGGCCTGGGTCGCGTGCGCACAATCCCTTACCCCCCGCGGaaaaac                                >  1:10263854/1‑146 (MQ=255)
                                                                                                                  ggAAGCGCCGTCGGCCGCTTTCGCGCCAGCCCTCGCGAAGGCTCGGCCCCTCACCTCCAGTGGTTGCAGATCGCCGAAGGCGCTCTGCATATCGCTCCGCTGCTCGTCCAGGGTCTCGTGCGCACATGCCATTCCCGCACGCGGAATAcc                                <  1:8257530/150‑1 (MQ=255)
                                                                                                                   gAAGCGCCGTCGGCCGCTTTCGCGCCAGCCCTCGCGAAGGCTCGGCCCCTCACCTCCAGTGGTTGCAGATCGCCGAAGGCGCTCTGCATATCGCTCCGCTGCTCGTCCTGGGTATCGTGCGCACATGCCATTCCCGCACGCGGAATACCg                               <  1:2230086/150‑1 (MQ=255)
                                                                                                                    aaGCGCCGTCGGCCGCTTTCGCGCCAGCCCTCGCGAAGGCTCGGCCCCTCACCTCCAGTGGTTGCAGATCGCCGAAGGCGCTCTGCATATCGCTCCGCTGCTCGTCCTGGGTCTCGTGCGCACATGCCATTCCCGCACGCGGAATACCGa                              <  1:8459182/150‑1 (MQ=255)
                                                                                                                      gcgcCGTCGGCCGCTTTCGCGCCAGCCCTCGCGAAGGCTCGGCCCCTCACCTCCCGTGGTTGCAGTTCGCCGAAGGCGCGCGGCAAATAGCGCCGCTGCGCGTCCAGGGGCGCGGGGGCACATGCCATTCACGCACGCGGGATACCGagt                            >  1:10477281/1‑148 (MQ=255)
                                                                                                                        gcCGTCGGCCGCTTTCGCGCCAGCCCTCGCGAAGGCTCGGCCCCTCACCTCCAGTGGTTGCAGATCGCCGAAGGCGCTCTGCATATCGCTCCGCTGCTCGTCCTGGGTCTCGTGCGCACATGCCATTCCCGCACGCGGAATACCGATAtt                          <  1:2653773/150‑1 (MQ=255)
                                                                                                                          cGTCGGCCGCTTTCGCGCCAGCCCTCGCGAAGGCTCGGCCCCTCACCTCCAGTGGTTGCAGATCGCCGAACGCGCGCAGCATATAGCTCCGCGGCTCGTCCTGGGGCGCGTGCGCCCATGCCCATCCCGCACGCGGAAAAACGATATTgg                        >  1:8831575/1‑149 (MQ=255)
                                                                                                                           gTCGGCCGCTTTCGCGCCAGCCCTCGCGAAGGCTCGGCCCCTCACCTCCAGTGGTTGCAGATCGCCGAAGGCGCTCTGCATATCGCTCCGCTGCTCGTCCTGGGTCTCGTGCGCACATGCCATTCCCGCACGCGGAATACCGATATTGCg                       <  1:5703328/150‑1 (MQ=255)
                                                                                                                            tCGGCCGCTTTCGCGCCAGCCCTCGCGAAGGCTCGGCCCCTCACCTCCAGTGGTTGCAGATCGCCGAAGGCGCTCTGCCTATAGCACCGCTGCGCGTCCGGGGTCTCGTGCGCACAAGCCAATCCCGCACGCGGAATACCGATATTGCGt                      >  1:6971331/1‑150 (MQ=255)
                                                                                                                            tCGGCCGCTTTCGCGCCAGCCCTCGCGAAGGCTCGGCCCCTCACCTCCAGTGGTTGCAGATCGCCGAAGGCGCTCTGCATATCGCTCCGCTGGTCGTGCGGGGTCTCGTGCGCACATTCCATTCCCGCACGCGGAATACCGATATTGCGt                      >  2:1468051/1‑150 (MQ=255)
                                                                                                                             cGGCCGCTTTCGCGCCAGCCCTCGCGAAGGCTCGGCCCCTCACCTCCAGTGGTTGCTGATAGCCGAAAGCGCGCTGCATTTCGCTCCGCGGCGCGTCCAGGGTGTCGTGCGCGCATGCCCATACCGCACGCGGAATACCCATAGTGCgga                     >  1:1769642/1‑148 (MQ=255)
                                                                                                                             cGGCCGCTTTCGCGCCAGCCCTCGCGAAGGCTCGGCCCCTCACCTCCAGTGGTTGCAGATCGCCGAAGGCGCTCTGCATATCGCTCCGCTGCTCGTCCTGGGTCTCGTGCGCACATGCCATTCCCGCACGCGGAATACCGATATTGCGTa                     <  2:3392030/150‑1 (MQ=255)
                                                                                                                              ggCCGCTTTCGCGCCAGCCCTCGCGAAGGCTCGGCCCCTCACCTCCAGTGGTTGCAGATCGCCGAAGGCGCTCTGCATATCGCTCCGCTGCTCGTCCTGGGTCTCGTGCGCACATGCCATTCCCGCACGCGGAATACCGATATTGCGTAt                    <  1:4178563/150‑1 (MQ=255)
                                                                                                                              ggCCGCTTTCGCGCCAGCCCTCGCGAAGGCTCGGCCCCTCACCTCCAGTGGTTGCAGATCGCCGAAGGCGCTCTGCATATCGCTCCGCTGCTCGTCCTGGGTCTCGTGCGCACATGCCATTCCCGCACGCGGAATACCGATATTGCGTAt                    <  1:9727457/150‑1 (MQ=255)
                                                                                                                               gCCGCTTTCGCGCCAGCCCTCGCGAAGGCTCGGCCCCTCACCTCCAGTGGTTGCAGATCGCCGAAGGCGCTCTGCATATCGCTCCGCTGCTCGTCCTGGGTCTCGTGCGCACATGCCATTCCCGCACGCGGAATACCGATATTGCGTATg                   <  1:6051333/150‑1 (MQ=255)
                                                                                                                               gCCGCTTTCGCGCCAGCCCTCGCGAAGGCTCGGCCCCTCACCTCCAGTGGTTGCAGATAGCCGAATGCGCGCTTCCTATAGCACCGCTGCAGGTCCGTGGTCTCGTGCGCACCAGCCAATCCCGCACGCGGGATACCGAGATTTAGTATg                   >  2:963850/1‑150 (MQ=255)
                                                                                                                                ccGCTTTCGCGCCAGCCCTCGCGAAGGCTCGGCCCCTCACCTCCAGTGGTTGCAGATCGCCGAAGGCGCTCTGCATATCGCTCCGCTGCTCGTCCTGGGTCTCGTGCGCACATGCCATTCCCGCACGCGGAATAACGATATTGCGTATgg                  >  2:7240416/1‑150 (MQ=255)
                                                                                                                                ccGCTTTCGCGCCAGCCCTCGCGAAGGCTCGGCCCCTCACCTCCAGTGGTTGCAGATCGCCGAAGGCGCACTGCATATCGCTCCGCTGCTCGTCCGGGGTCTCGTGCGCCCAAGCCAATCCCCCACCCGGAATACCGAAATTGCGGATgg                  >  1:10067450/1‑150 (MQ=255)
                                                                                                                                 cGCTTTCGCGCCAGCCCTCGCGAAGGCTCGGCCCCTCACCTCCAGTGGTTGCAGATCGCCGAAGGCGCTCTGCATATCGCTCCGCTGCTCGTCCTGGGTCTCGTGCGCCCATGCCAATCCCGCACGCGGAATACCGATATTGCGTTTggg                 >  2:1776925/1‑150 (MQ=255)
                                                                                                                                  gccttCGCGCCAGCCCTCGCGAAGGCTCGGCCCCTCCCCTCCAGTGGTTGCAGATCGCCGAAGGCGCTCTGCAAATCGCTCCGCTGCTCGTCCTGGGTCTCGTGCGCACATGCCATTCCCGCACGCGGAATACCGATATTGCGTATGGGc                <  2:5269411/147‑1 (MQ=255)
                                                                                                                                     ttCGCGCCAGCCCTCGCGAAGGCTCGGCCCCTCACCTCCAGTGGTTGCAGATAGCCGAAGGCGCTCTGCATATCGCTCCGCTGCTCGTCCGGGGTCTCGTGCGCACATGCCATTACCGCACGCGGAATACCGATATTGCGTATGGGCGaa             >  2:3184951/1‑150 (MQ=255)
                                                                                                                                      tCGCGCCAGCCCTCGCGAAGGCTCGGCCCCTCACCTCCAGTGGTTGCAGATCGCCGAAGGCGCTCTGCATATCGCTCCGCTGCTCGTCCTGGGTCTCGTGCGCACATGCCATTCCCGCACGCGGAATACCGATATTGCGTATGGGCGAAg            <  2:7727761/150‑1 (MQ=255)
                                                                                                                                      tCGCGCCAGCCCTCGCGAAGGCTCGGCCCCTCACCTCCAGTGGTTGCAGATCGCCGAAGGCGCTCTGCATATCGCTCCGCTGCTCGTCCGGGGTCTCGTGCGCACATGCCCTTCCCGCACGCGGAATACCGATATTGCGTATGGGCGAAg            >  2:9163742/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TCGGCGCGGAGGGCGAATTGAAGGCCGGGGTCGCCGACGGCGCCGCCGAGTTCACGTTTACCTTCCCCTGAGCGGCGCCGCCGCTCTTTCCCCGGCGTTTCCCGCCCCGCCTCGGGAAGCGCCGTCGGCCGCTTTCGCGCCAGCCCTTGCGAAGGCTCGGCCCCTCACCTCCAGTGGTTGCAGATCGCCGAAGGCGCTCTGCATATCGCTCCGCTGCTCGTCCTGGGTCTCGTGCGCACATGCCATTCCCGCACGCGGAATACCGATATTGCGTATGGGCGAAGGCTTTCTGAG  >  NC_008463/966070‑966363

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑