BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_008463	1,239,152	N→C	intergenic (+150/‑43)	PA14_RS05795 → / → lptF	alpha/beta hydrolase/LPS export ABC transporter permease LptF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_008463	1,239,152	0	N	C	100.0%	1919.9 / NA	497	intergenic (+150/‑43)	PA14_RS05795/lptF	alpha/beta hydrolase/LPS export ABC transporter permease LptF
Reads supporting (aligned to +/- strand): ref base N (0/0); new base C (255/242); total (255/242)

Only 100 of 500 total aligned reads displayed.

TCGGCAAGCGCTGAACGAGCGTTCGCCTCGACGGTCTATCGGGCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCNGACGGGCCCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATCATCATGAGTGGCCGTTTCATCAAGTACCTGGC  >  NC_008463/1239004‑1239301

 cGGCAAGCGCTGAACGAGCGTTCGCCTCGACGGTCTATCGGGCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGa                                                                                                                                                     <  2:6822677/150‑1 (MQ=255)

  ggCAAGCGCTGAACGAGCGTTCGCCTCGACGGTCTATCGGGCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGAc                                                                                                                                                    <  2:6369115/150‑1 (MQ=255)

  ggCAAGCGCTGAACGAGCGTTCGCCTCGACGGTCTATCGGGCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATGTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGAc                                                                                                                                                    <  1:8301687/150‑1 (MQ=255)

   gCAAGCGCTGAACGAGCGTTCGCCTCGACGGTCTATCGGGCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACg                                                                                                                                                   >  2:10550997/1‑150 (MQ=255)

      aGCGCTGAACGAGCGTTCGCCTCGACGGTCTATCGGGCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGGc                                                                                                                                                >  2:4602651/1‑150 (MQ=255)

         gcTGAACGAGCGTTCGCCTCGACGGTCTATCGGGCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGGcccc                                                                                                                                             >  1:8929820/1‑150 (MQ=255)

           tGAACGAGCGTTCGCCTCGACGGTCTATCGGGCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGGccccgt                                                                                                                                           >  2:7462629/1‑148 (MQ=255)

             aaCGAGCGTTCGCCTCGACGGTCTATCGGGCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGGccccgtca                                                                                                                                         >  2:716078/1‑146 (MQ=255)

                 aGCGTTCGCCTCGACGGTCTATCGGGCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGc                                                                                                                                    >  2:6150310/1‑150 (MQ=255)

                  gCGTTCGCCTCGACGGTCTATCGGGCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTACTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGcc                                                                                                                                   <  2:2677607/150‑1 (MQ=255)

                    gTTCGCCTCGACGGTCTATCGGGCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTg                                                                                                                                 <  1:10608247/150‑1 (MQ=255)

                    gTTCGCCTCGACGGTCTATCGGGCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTg                                                                                                                                 >  1:3246605/1‑150 (MQ=255)

                     ttCGCCTCGACGGTCTATCGGGCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTgg                                                                                                                                <  2:3441815/150‑1 (MQ=255)

                        gCCTCGACGGTCTATCGGGCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCaa                                                                                                                             >  1:1369043/1‑150 (MQ=255)

                        gCCTCGACGGTCTATCGGGCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCaa                                                                                                                             <  2:3192988/150‑1 (MQ=255)

                        gCCTCGACGGTCTATCGGGCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCaa                                                                                                                             >  1:4893831/1‑150 (MQ=255)

                         ccTCGACGGTCTATCGGGCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGACACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAAc                                                                                                                            >  1:6030464/1‑150 (MQ=255)

                         ccTCGACGGTCTATCGGGCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAAc                                                                                                                            >  2:6050133/1‑150 (MQ=255)

                            cGACGGTCTATCGGGCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCa                                                                                                                         <  1:10180088/150‑1 (MQ=255)

                             gACGGTCTATCGGGCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAg                                                                                                                        >  1:1489430/1‑150 (MQ=255)

                             gACGGTCTATCGGGCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAg                                                                                                                        >  2:1656802/1‑150 (MQ=255)

                                 gTCTATCGGGCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAAAGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAgt                                                                                                                    >  1:77709/1‑150 (MQ=255)

                                     aTCGGGCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTcc                                                                                                                <  1:210421/150‑1 (MQ=255)

                                     aTCGGGCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTcc                                                                                                                <  2:9495674/150‑1 (MQ=255)

                                         ggCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGAt                                                                                                            <  2:2962016/150‑1 (MQ=255)

                                         ggCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGAt                                                                                                            <  1:10266289/150‑1 (MQ=255)

                                           ctctTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTg                                                                                                          <  2:8218127/150‑1 (MQ=255)

                                             ctTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGAt                                                                                                        <  2:5744180/150‑1 (MQ=255)

                                              ttGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATc                                                                                                       <  1:10934822/150‑1 (MQ=255)

                                              ttGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATc                                                                                                       <  1:8810197/150‑1 (MQ=255)

                                                 gTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGtt                                                                                                    >  1:1618666/1‑150 (MQ=255)

                                                  ttCGGCCTGCGCAGGGACAGGCCCGCCGAATGGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGTGCCTGGCAACCCAGGAGTGTCCGGATTGATCGttt                                                                                                   <  2:5658519/150‑1 (MQ=255)

                                                      gCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCg                                                                                               >  2:4521024/1‑150 (MQ=255)

                                                      gCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCg                                                                                               <  1:2821368/150‑1 (MQ=255)

                                                         tGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTa                                                                                            <  1:8697041/150‑1 (MQ=255)

                                                             cAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTg                                                                                        >  1:3114225/1‑150 (MQ=255)

                                                              aGTGACCGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGt                                                                                       <  1:10694057/150‑1 (MQ=255)

                                                                     ggCCCGCCGACTCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGa                                                                                >  2:210730/1‑150 (MQ=255)

                                                                     ggCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGa                                                                                <  2:132551/150‑1 (MQ=255)

                                                                     ggCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGa                                                                                >  2:4960553/1‑150 (MQ=255)

                                                                       cccGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAg                                                                              <  1:435600/150‑1 (MQ=255)

                                                                           ccgAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCt                                                                          >  1:2675620/1‑150 (MQ=255)

                                                                            cgAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTg                                                                         <  2:8740414/150‑1 (MQ=255)

                                                                             gAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTgg                                                                        >  2:6513072/1‑150 (MQ=255)

                                                                                     ggATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATg                                                                >  1:8165181/1‑150 (MQ=255)

                                                                                     ggATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATg                                                                <  2:2346910/150‑1 (MQ=255)

                                                                                      gATAATGCCGGCTTTTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGTTGTCGGGGCCGACGGG‑CCGCGTCAGAGACTGGCAACCCATGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGATGGTCACCATGa                                                               <  2:3597708/150‑1 (MQ=255)

                                                                                      gATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGa                                                               >  2:427827/1‑150 (MQ=255)

                                                                                      gATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGa                                                               >  2:5617343/1‑150 (MQ=255)

                                                                                       aTAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAg                                                              <  1:4739506/150‑1 (MQ=255)

                                                                                       aTAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAg                                                              <  2:11001139/150‑1 (MQ=255)

                                                                                          aTGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAgcgc                                                           >  2:1558297/1‑150 (MQ=255)

                                                                                           tGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGACACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGcc                                                          >  2:4140435/1‑150 (MQ=255)

                                                                                           tGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGcc                                                          <  2:8878470/150‑1 (MQ=255)

                                                                                           tGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGcc                                                          >  1:1848872/1‑150 (MQ=255)

                                                                                             ccGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGt                                                        >  2:6641589/1‑150 (MQ=255)

                                                                                             ccGGCTATTTTCTCCGCGCCATGCCGCCAGGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGt                                                        <  2:10029359/150‑1 (MQ=255)

                                                                                               ggCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCGGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCa                                                      >  2:2468741/1‑150 (MQ=255)

                                                                                               ggCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCa                                                      <  1:9317691/150‑1 (MQ=255)

                                                                                                gcgatgttCTCCGCGCCCAGCCGCCATGGCCGGAAACGCGTGCTGCCGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCACCCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAg                                                     <  2:9651667/144‑1 (MQ=255)

                                                                                                gCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATGGATCGTTTTCCGGTCTTTGTCCCGTGAAGTGCTGGTCACCCTGAGCGCCGTCAg                                                     >  2:9720997/1‑150 (MQ=255)

                                                                                                 cTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAgc                                                    <  1:1907913/150‑1 (MQ=255)

                                                                                                     tttCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCg                                                >  1:4182940/1‑150 (MQ=255)

                                                                                                     tttCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGGCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCg                                                <  1:9933317/150‑1 (MQ=255)

                                                                                                      ttCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGt                                               >  1:6243210/1‑150 (MQ=255)

                                                                                                       tctcCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGtg                                              >  1:5252092/1‑150 (MQ=255)

                                                                                                           cgcgCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGtgctgc                                          >  1:8472342/1‑150 (MQ=255)

                                                                                                            gcgcCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTgctgct                                         <  1:3447244/150‑1 (MQ=255)

                                                                                                              gcCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTgg                                       >  2:1779530/1‑150 (MQ=255)

                                                                                                               ccATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGt                                      >  2:3846045/1‑150 (MQ=255)

                                                                                                                 aTGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGa                                    >  1:2199770/1‑150 (MQ=255)

                                                                                                                 aTGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGa                                    >  1:7706696/1‑150 (MQ=255)

                                                                                                                  tGCCGCCATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGat                                   >  1:5684036/1‑150 (MQ=255)

                                                                                                                   gccgccATGGCCGGAAACGCGTGATGACGGGGCCGCCGGG‑CCCCGTCAGAGCCGGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGatc                                  <  2:5488479/150‑1 (MQ=255)

                                                                                                                   gccgccATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGatc                                  <  2:10259658/150‑1 (MQ=255)

                                                                                                                     cgccATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGatcat                                <  1:3764299/150‑1 (MQ=255)

                                                                                                                      gccATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGatcatc                               >  1:9652362/1‑150 (MQ=255)

                                                                                                                      gccATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGatcatc                               >  2:8379361/1‑150 (MQ=255)

                                                                                                                       ccATGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGAtcatca                              <  1:7318698/150‑1 (MQ=255)

                                                                                                                          tGGCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATCATCATGa                           >  1:3317716/1‑150 (MQ=255)

                                                                                                                            gCCGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATCATCATGAGt                         <  1:6614143/150‑1 (MQ=255)

                                                                                                                             ccGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGAGTGATCGTTTTCCGTTATTTGTCCCGGGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGTTCATCATGAGTg                        >  1:5432801/1‑150 (MQ=255)

                                                                                                                              cGGAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATCATCATGAGTgg                       <  2:8054914/150‑1 (MQ=255)

                                                                                                                                gAAACGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGGTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATCATCATGAGTGGcc                     >  2:9196423/1‑150 (MQ=255)

                                                                                                                                  aaCGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATCATCATGAGTGGCCGt                   <  1:3889272/150‑1 (MQ=255)

                                                                                                                                  aaCGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATCATCATGAGTGGCCGt                   <  2:4001760/150‑1 (MQ=255)

                                                                                                                                   aCGCGTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATCATCATGAGTGGCCGtt                  >  2:7850112/1‑150 (MQ=255)

                                                                                                                                      cgTGATGACGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATCATCATGAGTGGCCGTTtca               <  1:4550560/150‑1 (MQ=255)

                                                                                                                                         gatgaCGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATCATCATGAGTGGCCGTTtcatca            >  1:6814729/1‑150 (MQ=255)

                                                                                                                                           tgaCGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATCATCATGAGTGGCCGTTTCATCAAg          <  1:10023780/150‑1 (MQ=255)

                                                                                                                                            gaCGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCGACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATCATCATGAGTGGCCGTTTCATCAAGt         <  1:7988560/150‑1 (MQ=255)

                                                                                                                                             aCGGGGCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATCATCATGAGTGGCCGTTTCATCAAGTa        <  1:5733580/150‑1 (MQ=255)

                                                                                                                                               ggggCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATCATCATGAGTGGCCGTTTCATCAAGTAcc      >  2:8971545/1‑150 (MQ=255)

                                                                                                                                                gggCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATCATCATGAGTGGCCGTTTCATCAAGTACCt     <  1:445385/150‑1 (MQ=255)

                                                                                                                                                gggCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATCATCATGAGTGGCCGTTTCATCAAGTACCt     <  1:6429096/150‑1 (MQ=255)

                                                                                                                                                gggCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATCATCATGAGTGGCCGTTTCATCAAGTACCt     <  2:10760607/150‑1 (MQ=255)

                                                                                                                                                gggCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGGCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATCATCATGAGTGGCCGTTTCATCAAGTACCt     >  2:1043736/1‑150 (MQ=255)

                                                                                                                                                  gCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATCATCATGAGTGGCCGTTTCATCAAGTCCCTgg   <  2:5060886/150‑1 (MQ=255)

                                                                                                                                                  gCCGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATCATCATGAGTGGCCGTTTCATCAAGTACCTgg   >  1:8981151/1‑150 (MQ=255)

                                                                                                                                                   ccGACGGG‑CCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATCATCATGAGTGGCCGTTTCATCAAGTACCTGgc  <  1:2434933/150‑1 (MQ=255)

TCGGCAAGCGCTGAACGAGCGTTCGCCTCGACGGTCTATCGGGCTCTTGGTTCGGCCTGCGCAGTGACAGGCCCGCCGAATCGCAGGATAATGCCGGCTATTTTCTCCGCGCCATGCCGCCATGGCCGGAAACGCGTGATGACGGGGCNGACGGGCCCCCGTCAGAGCCTGGCAACCCAGGAGTGTCCGGATTGATCGTTTTCCGTTATTTGTCCCGTGAAGTGCTGGTCACCATGAGCGCCGTCAGCGCCGTGCTGCTGGTGATCATCATGAGTGGCCGTTTCATCAAGTACCTGGC  >  NC_008463/1239004‑1239301

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`