Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 1,375,947 A→G intergenic (+18/‑38) PA14_RS06470 → / → PA14_RS06475 PspA/IM30 family protein/DUF1449 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084631,375,9470AG100.0% 1725.0 / NA 440intergenic (+18/‑38)PA14_RS06470/PA14_RS06475PspA/IM30 family protein/DUF1449 family protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (199/241);  total (199/241)

Only 100 of 444 total aligned reads displayed.

GGCCGAACTGGCCAGCGCGGCGGCGCCGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCAAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCTACTGGGGCATCGTCGCCCTGGGCATGGTCGAGA  >  NC_008463/1375800‑1376093
                                                                                                                                                   |                                                                                                                                                  
ggCCGAACTGGCCAGCGCGGCGGCGCCGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCgaa                                                                                                                                                  >  2:4474709/1‑150 (MQ=255)
    gAACTGGCCAGCGCGGCGGCGCCGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCg                                                                                                                                              <  2:7777661/150‑1 (MQ=255)
     aaCTGGCCAGCGCGGCGGCGCCGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGt                                                                                                                                             >  1:3827752/1‑150 (MQ=255)
     aaCTGGCCAGCGCGGCGGCGCCGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGt                                                                                                                                             >  2:7212147/1‑150 (MQ=255)
        tGGCCAGCGCGGCGGCGCCGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAATGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCg                                                                                                                                          <  2:694124/150‑1 (MQ=255)
        tGGCCAGCGCGGCGGCGCCGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCg                                                                                                                                          <  2:1909226/150‑1 (MQ=255)
         ggCCAGCGCGGCGGCGCCGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCgg                                                                                                                                         >  2:6015440/1‑150 (MQ=255)
         ggCCAGCGCGGCGGCGCCGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCgg                                                                                                                                         >  1:4372248/1‑150 (MQ=255)
           ccAGCGCGGCGGCGCCGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGc                                                                                                                                       <  1:4547340/150‑1 (MQ=255)
            cAGCGCGGCGGCGCCGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGcc                                                                                                                                      <  1:4761936/150‑1 (MQ=255)
                 cggcggcgCCGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGttt                                                                                                                                 >  1:4726279/1‑150 (MQ=255)
                 cggcggcgCCGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGttt                                                                                                                                 <  1:4664168/150‑1 (MQ=255)
                 cggcggcgCCGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGttt                                                                                                                                 >  1:3015233/1‑150 (MQ=255)
                  ggcggcgCCGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCGGAAGGAATAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGTCGGCGTCCGGGCCCGtttt                                                                                                                                >  1:7685460/1‑150 (MQ=255)
                    cggcgCCGGACGCTGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTc                                                                                                                              <  2:5433316/150‑1 (MQ=255)
                    cggcgCCGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTc                                                                                                                              <  1:9272449/150‑1 (MQ=255)
                       cgcCGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAgg                                                                                                                           <  1:1245341/150‑1 (MQ=255)
                       cgcCGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAgg                                                                                                                           <  1:7202847/150‑1 (MQ=255)
                         ccGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGa                                                                                                                         <  1:10611036/150‑1 (MQ=255)
                            gACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAGAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATag                                                                                                                      <  1:10768048/150‑1 (MQ=255)
                            gACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATag                                                                                                                      <  1:10198437/150‑1 (MQ=255)
                             aCGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATaga                                                                                                                     >  2:8057498/1‑150 (MQ=255)
                                  gcgcTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAATGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGAc                                                                                                                >  1:9617737/1‑150 (MQ=255)
                                     cTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAAt                                                                                                             >  2:4590199/1‑150 (MQ=255)
                                      tGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATg                                                                                                            <  2:2178051/150‑1 (MQ=255)
                                      tGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATg                                                                                                            <  1:2358189/150‑1 (MQ=255)
                                      tGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATg                                                                                                            <  1:1716096/150‑1 (MQ=255)
                                       ggACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCGGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGACGGCGTCCGGGCCCGTGTGTCAGGGATAGAGCGACACTgg                                                                                                           >  2:2685793/1‑150 (MQ=255)
                                        gACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATggt                                                                                                          >  1:4910132/1‑149 (MQ=255)
                                            ccAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGGCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGt                                                                                                      >  1:6063442/1‑150 (MQ=255)
                                              aaGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCTGGGATAGAGCGACAATGGAAGGttt                                                                                                    >  2:10598893/1‑150 (MQ=255)
                                              aaGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGttt                                                                                                    <  2:8936745/150‑1 (MQ=255)
                                              aaGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGttt                                                                                                    >  1:9852211/1‑150 (MQ=255)
                                                   gcgcgcGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGc                                                                                               <  1:5444132/150‑1 (MQ=255)
                                                    cgcgcgGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCa                                                                                              <  1:1878944/150‑1 (MQ=255)
                                                       gcgGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGAc                                                                                           >  1:6596310/1‑150 (MQ=255)
                                                        cgGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACg                                                                                          <  1:8379134/150‑1 (MQ=255)
                                                        cgGCCGGGATCAAGGCCGCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGACAAGGGCCAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTGTCCTGCAGACg                                                                                          <  2:7840304/150‑1 (MQ=255)
                                                          gCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGgc                                                                                        >  2:5176068/1‑150 (MQ=255)
                                                            cGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGgcgc                                                                                      >  2:4220871/1‑150 (MQ=255)
                                                            cGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGATTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGgcgc                                                                                      <  2:6188384/150‑1 (MQ=255)
                                                            cGGGATCAAGGCCTCGGGCAACAGAGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATCGAGCGACAATGGAAGGTTTCCTGCAGACGgcgc                                                                                      <  2:5802630/150‑1 (MQ=255)
                                                             gggATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCACGGCCGGATAGGCCCGCGGCCCGGCGTCGGCGTCCGGGCCCGGTTTTCAGGGATAGAGCGACAATGGCAGGTTTCGTGCAGACTGCGCt                                                                                     >  2:10745589/1‑150 (MQ=255)
                                                             gggATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCt                                                                                     >  1:3124562/1‑150 (MQ=255)
                                                               gATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGt                                                                                   >  1:3663052/1‑150 (MQ=255)
                                                                aTCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTc                                                                                  <  2:1104137/150‑1 (MQ=255)
                                                                aTCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTc                                                                                  <  2:9441275/150‑1 (MQ=255)
                                                                   aaGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGTATAGAGCGACAAAGGAAGGTTTCCTGCAGACGGCGCTGTCGtt                                                                               >  2:907815/1‑150 (MQ=255)
                                                                     ggCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACACTGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTc                                                                             >  2:7124815/1‑150 (MQ=255)
                                                                              cAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGc                                                                    <  2:8460164/150‑1 (MQ=255)
                                                                                aCAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTGTCCGACCGTGCTg                                                                  >  1:4106928/1‑150 (MQ=255)
                                                                                aCAGCGTGGATGGCGAGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTg                                                                  <  2:9671528/150‑1 (MQ=255)
                                                                                 cAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGTGATAGAGCGAGAATGGAAGGTTTCCTGCAGTCGGCGCTGTCGTTTCCGACCGTGCTGt                                                                 >  2:10884939/1‑150 (MQ=255)
                                                                                  aGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGtt                                                                >  1:705288/1‑150 (MQ=255)
                                                                                   gCGTGGATGGCGTGCTGGCGCGCCTGGAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGCAGGTTTCCTGCAGACGGCGCTGTCGTTGCCGACCGTGGTGTTc                                                               >  2:1392732/1‑150 (MQ=255)
                                                                                   gCGTGGATGGCGTGCTGGCGCGCCAGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTc                                                               <  1:1008203/150‑1 (MQ=255)
                                                                                     gTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAg                                                             <  2:47929/150‑1 (MQ=255)
                                                                                     gTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAg                                                             >  1:8171273/1‑150 (MQ=255)
                                                                                       ggATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCt                                                           <  1:2600827/150‑1 (MQ=255)
                                                                                        gATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCtt                                                          >  2:3936505/1‑150 (MQ=255)
                                                                                        gATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCtt                                                          <  1:215737/150‑1 (MQ=255)
                                                                                        gATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTGCAGCtt                                                          >  2:869621/1‑150 (MQ=255)
                                                                                              gTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTtcttct                                                    <  1:7340617/150‑1 (MQ=255)
                                                                                               tGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTg                                                   >  2:1519397/1‑150 (MQ=255)
                                                                                               tGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTg                                                   >  2:566168/1‑150 (MQ=255)
                                                                                                  tGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATc                                                >  1:3514236/1‑150 (MQ=255)
                                                                                                   ggCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATcc                                               >  2:2379740/1‑150 (MQ=255)
                                                                                                   ggCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATcc                                               >  2:10008/1‑150 (MQ=255)
                                                                                                     cgcgcCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTc                                             <  2:2849665/150‑1 (MQ=255)
                                                                                                      gcgcCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTcg                                            <  1:11034319/150‑1 (MQ=255)
                                                                                                          cTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGat                                        <  2:3704088/150‑1 (MQ=255)
                                                                                                           tGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGatc                                       >  1:8876820/1‑150 (MQ=255)
                                                                                                            gAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGatca                                      >  1:2386476/1‑150 (MQ=255)
                                                                                                            gAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGatca                                      >  2:9930558/1‑150 (MQ=255)
                                                                                                            gAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGatca                                      >  1:5645012/1‑150 (MQ=255)
                                                                                                            gAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGatca                                      <  1:6863217/150‑1 (MQ=255)
                                                                                                              aGGAATAGGGCAAGGCCTGATAGGCCCTCGGCCCGGCGCAGGCGTCCGGTCCCGTTTTTCAGGGATAGAGCGACTATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGatcatc                                    <  1:949079/150‑1 (MQ=255)
                                                                                                              aGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGatcatc                                    >  1:8771505/1‑150 (MQ=255)
                                                                                                              aGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGatcatc                                    <  1:5106482/150‑1 (MQ=255)
                                                                                                               ggAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCt                                   <  1:2729390/150‑1 (MQ=255)
                                                                                                                  aaaGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCTACt                                >  2:10149636/1‑150 (MQ=255)
                                                                                                                  aaaGGGCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCTACt                                <  1:9371902/150‑1 (MQ=255)
                                                                                                                    aGGGCAAGGCCTGCTAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCTACTgg                              <  2:6391676/150‑1 (MQ=255)
                                                                                                                      ggCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCTACTgggg                            <  2:8800234/150‑1 (MQ=255)
                                                                                                                       gCAAGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAAGGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCTACTGGGGc                           <  2:3184923/150‑1 (MQ=255)
                                                                                                                         aaGGCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCTACTGGGGCAt                         <  2:3318018/150‑1 (MQ=255)
                                                                                                                            gCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCTACTGGGGCAtcgt                      <  1:156492/150‑1 (MQ=255)
                                                                                                                            gCCTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCTACTGGGGCAtcgt                      <  1:10274456/150‑1 (MQ=255)
                                                                                                                             ccTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCTACTGGGGCAtcgtc                     >  1:5340181/1‑150 (MQ=255)
                                                                                                                              cTGATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCTACTGGGGCAtcgtcg                    >  1:7811534/1‑150 (MQ=255)
                                                                                                                                gATAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCTACTGGGGCATCGTCGcc                  >  1:7938071/1‑150 (MQ=255)
                                                                                                                                  tAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCTACTGGGGCATCGTCGCCCt                >  2:7350969/1‑150 (MQ=255)
                                                                                                                                  tAGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCTACTGGGGCATCGTCGCCCt                >  2:9205268/1‑150 (MQ=255)
                                                                                                                                   aGGCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCTACTGGGGCATCGTCGCCCTg               >  1:4976106/1‑150 (MQ=255)
                                                                                                                                     gCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCTACTGGGGCATCGTCGCCCTggg             <  2:8208485/150‑1 (MQ=255)
                                                                                                                                     gCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCTACTGGGGCATCGTCGCCCTggg             <  2:3015233/150‑1 (MQ=255)
                                                                                                                                     gCCCGCGGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCTACTGGGGCATCGTCGCCCTggg             >  2:4295689/1‑150 (MQ=255)
                                                                                                                                        cgcgGCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCTACTGGGGCATCGTCGCCCTGGGCAt          >  2:1236566/1‑150 (MQ=255)
                                                                                                                                           ggCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTGTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCTACTGGGGCATCGTCGCCCTGGGCATGGt       <  2:163359/150‑1 (MQ=255)
                                                                                                                                            gCCCGGCGAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCTACTGGGGCATCGTCGCCCTGGGCATGGTc      >  1:2786061/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GGCCGAACTGGCCAGCGCGGCGGCGCCGGACGATGCGCTGGACGCCAAGCTGCGCGCGGCCGGGATCAAGGCCTCGGGCAACAGCGTGGATGGCGTGCTGGCGCGCCTGAAGGAAAAGGGCAAGGCCTGATAGGCCCGCGGCCCGGCAAAGGCGTCCGGGCCCGTTTTTCAGGGATAGAGCGACAATGGAAGGTTTCCTGCAGACGGCGCTGTCGTTTCCGACCGTGCTGTTCAGCTTTCTTCTGATCCTCGCGATCATCTACTGGGGCATCGTCGCCCTGGGCATGGTCGAGA  >  NC_008463/1375800‑1376093

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑