Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 1,880,872 C→G A502G (GCC→GGC)  PA14_RS08740 → DEAD/DEAH box helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084631,880,8720CG99.8% 2097.9 / NA 531A502G (GCC→GGC) PA14_RS08740DEAD/DEAH box helicase
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base G (277/253);  total (278/253)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 538 total aligned reads displayed.

TCCGCGCCGAGCCCTACGCCGGGCTGGAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGCCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGCCTGGCCGGCGACGTGTTCCAACTGG  >  NC_008463/1880725‑1881018
                                                                                                                                                   |                                                                                                                                                  
tCCGCGCCGAGCCCTACGCCGGGCTGGAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGcg                                                                                                                                                  >  1:2386023/1‑150 (MQ=255)
tCCGCGCCGAGCCCTACGCCGGGCTGGAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGcg                                                                                                                                                  >  1:4561693/1‑150 (MQ=255)
   gcgcCGAGCCCTACGCCGGGCTGGAGCGCGCGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTCTCTCCCTCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGgcgcgc                                                                                                                                               >  2:10251515/1‑150 (MQ=255)
   gcgcCGAGCCCTACGCCGGGCTGGAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGgcgcgc                                                                                                                                               <  2:3586385/150‑1 (MQ=255)
   gcgcCGAGCCCTACGCCGGGCTGGAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGgcgcgc                                                                                                                                               <  1:9002855/150‑1 (MQ=255)
    cgcCGAGCCCTACGCCGGGCTGGAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGcgcgcg                                                                                                                                              >  2:8036926/1‑150 (MQ=255)
     gcCGAGCCCTACGCCGGGCTGGAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCgg                                                                                                                                             >  2:2985565/1‑150 (MQ=255)
      ccGAGCCCTACGCCGGGCTGGAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGc                                                                                                                                            <  1:5161092/150‑1 (MQ=255)
       cGAGCCCTACGCCGGGCTGGAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCt                                                                                                                                           <  1:6154153/150‑1 (MQ=255)
          gCCCTACGCCGGGCTGGAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCGACCACTCACGACTCGGCGTGCGCGGCGCCTATCTCCATCGGGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGAc                                                                                                                                        <  2:7020053/150‑1 (MQ=255)
           cccTACGCCGGGCTGGAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGAcc                                                                                                                                       >  1:741263/1‑150 (MQ=255)
            ccTACGCCGGGCTGGAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACcg                                                                                                                                      <  2:6148591/150‑1 (MQ=255)
            ccTACGCCGGGCTGGAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACcg                                                                                                                                      <  2:5278671/150‑1 (MQ=255)
             cTACGCCGGGCTGTAGCGCGAGCGCTTCGACGAAGTCCAGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGAGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACcgc                                                                                                                                     >  1:6874740/1‑150 (MQ=255)
             cTACGCCGGGCTGGAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACcgc                                                                                                                                     >  2:1464974/1‑150 (MQ=255)
               aCGCCGGGCTGGAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCgcgc                                                                                                                                   >  1:9636551/1‑150 (MQ=255)
               aCGCCGGGCTGGAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCgcgc                                                                                                                                   >  1:5346150/1‑150 (MQ=255)
                cGCCGGGCTGGAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCt                                                                                                                                  <  2:8833726/150‑1 (MQ=255)
                   cGGGCTGGAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGAc                                                                                                                               >  2:5385275/1‑150 (MQ=255)
                    gggCTGGAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGAcc                                                                                                                              <  1:2795936/150‑1 (MQ=255)
                    gggCTGGAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGAcc                                                                                                                              >  1:6646530/1‑150 (MQ=255)
                      gCTGGAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTc                                                                                                                            <  1:7826558/150‑1 (MQ=255)
                         ggAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGGGGCCGCCGGGGCGCGCGGCTGACCGCGCTGACCTcggg                                                                                                                         >  2:7619208/1‑150 (MQ=255)
                          gagcgcgagcgcTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTcgggc                                                                                                                        >  1:4972314/1‑150 (MQ=255)
                          gagcgcgagcgcTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTcgggc                                                                                                                        >  2:967796/1‑150 (MQ=255)
                             cgcgagcgcTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTcgggcggg                                                                                                                     <  1:8950630/150‑1 (MQ=255)
                                    gcTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTc                                                                                                              <  1:8494286/150‑1 (MQ=255)
                                    gcTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTc                                                                                                              <  2:7187729/150‑1 (MQ=255)
                                    gcTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTc                                                                                                              <  1:3433139/150‑1 (MQ=255)
                                        cgacgaAGTCCTGCGGATGCGCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGCTGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGa                                                                                                          <  2:5166106/150‑1 (MQ=255)
                                           cgaAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATAc                                                                                                       <  1:1945604/150‑1 (MQ=255)
                                            gaAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATAcc                                                                                                      <  1:4839655/150‑1 (MQ=255)
                                             aaGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCg                                                                                                     <  2:3355754/150‑1 (MQ=255)
                                              aGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCgg                                                                                                    >  2:2609746/1‑150 (MQ=255)
                                                  cTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGAc                                                                                                <  2:4870880/150‑1 (MQ=255)
                                                     cGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTAc                                                                                             <  2:1784695/150‑1 (MQ=255)
                                                         tGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCg                                                                                         <  1:6032265/150‑1 (MQ=255)
                                                          gCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATGCCCGATACCGGCGACTACAGCGt                                                                                        >  2:10948029/1‑150 (MQ=255)
                                                            tCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGtgc                                                                                      <  2:9424158/150‑1 (MQ=255)
                                                               ccGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGtgctgc                                                                                   <  2:3983262/150‑1 (MQ=255)
                                                               ccGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCACCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGtgctgc                                                                                   <  1:1584876/150‑1 (MQ=255)
                                                                 gAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGctgctg                                                                                 >  1:4395910/1‑150 (MQ=255)
                                                                   gggCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGa                                                                               >  1:10564416/1‑150 (MQ=255)
                                                                      cTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGcc                                                                            <  1:10181337/150‑1 (MQ=255)
                                                                       tACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGCTGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCg                                                                           <  2:6036068/150‑1 (MQ=255)
                                                                          cACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAg                                                                        >  1:6426069/1‑150 (MQ=255)
                                                                           aCTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAgg                                                                       <  1:11037678/150‑1 (MQ=255)
                                                                              cACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGCTGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCAGTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGcc                                                                    <  1:3851267/150‑1 (MQ=255)
                                                                               aCGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCct                                                                   <  1:5048304/150‑1 (MQ=255)
                                                                               aCGCCTCGGCGTGCGCGGCGCCTATCACCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCTGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCct                                                                   >  1:9328853/1‑150 (MQ=255)
                                                                                 gCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCctgc                                                                 <  2:6581849/150‑1 (MQ=255)
                                                                                 gCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCctgc                                                                 <  2:1255125/150‑1 (MQ=255)
                                                                                  ccTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCctgcg                                                                >  1:10195731/1‑149 (MQ=255)
                                                                                   cTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCctgctg                                                               >  2:5604782/1‑150 (MQ=255)
                                                                                    tCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCtgg                                                              <  2:1996559/150‑1 (MQ=255)
                                                                                    tCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGAGACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCtgg                                                              >  1:5712436/1‑150 (MQ=255)
                                                                                          tGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGaa                                                        >  2:7372502/1‑150 (MQ=255)
                                                                                          tGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCGGGTGGGac                                                        >  1:310581/1‑149 (MQ=255)
                                                                                            cgcgGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCGTGCTGGTGGGAACg                                                      >  1:560669/1‑150 (MQ=255)
                                                                                                gcgcCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCa                                                  >  1:6521700/1‑150 (MQ=255)
                                                                                                 cgcCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCaa                                                 <  2:7418358/150‑1 (MQ=255)
                                                                                                 cgcCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCaa                                                 <  2:4327205/150‑1 (MQ=255)
                                                                                                  gcCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAAc                                                <  1:10034448/150‑1 (MQ=255)
                                                                                                   ccTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACg                                               >  1:139398/1‑150 (MQ=255)
                                                                                                     tATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAg                                             <  1:10867566/150‑1 (MQ=255)
                                                                                                     tATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAg                                             >  1:4493912/1‑150 (MQ=255)
                                                                                                     tATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGACAACGAg                                             >  2:4623600/1‑150 (MQ=255)
                                                                                                        ctcCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGAc                                          <  1:7187161/150‑1 (MQ=255)
                                                                                                        ctcCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGAc                                          >  1:7337519/1‑150 (MQ=255)
                                                                                                         tcCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACt                                         >  1:4093894/1‑150 (MQ=255)
                                                                                                         tcCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACt                                         >  1:5880850/1‑150 (MQ=255)
                                                                                                          ccATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACtt                                        <  1:1902505/150‑1 (MQ=255)
                                                                                                             tCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGCCCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTcgc                                     <  2:6943389/150‑1 (MQ=255)
                                                                                                              cgcgATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTcgcg                                    >  2:1286922/1‑150 (MQ=255)
                                                                                                              cgcgATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGAGACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTcgcg                                    <  1:2472242/150‑1 (MQ=255)
                                                                                                               gcgATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCgg                                   <  2:2332573/150‑1 (MQ=255)
                                                                                                                 gATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTg                                 >  1:10715460/1‑150 (MQ=255)
                                                                                                                     cgcTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGgaga                             <  2:10037267/150‑1 (MQ=255)
                                                                                                                      gcTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGagag                            <  2:8540172/150‑1 (MQ=255)
                                                                                                                       cTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGc                           >  2:8380743/1‑150 (MQ=255)
                                                                                                                       cTCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGc                           >  1:10419318/1‑150 (MQ=255)
                                                                                                                        tCAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGcc                          <  1:3729491/150‑1 (MQ=255)
                                                                                                                         cAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATCCCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGCCt                         <  1:3235666/150‑1 (MQ=255)
                                                                                                                         cAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATCCCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGCCt                         <  1:473201/150‑1 (MQ=255)
                                                                                                                         cAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGCCt                         >  1:9124691/1‑150 (MQ=255)
                                                                                                                         cAACGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCAGTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGCCt                         <  2:10439463/150‑1 (MQ=255)
                                                                                                                          aaCGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGTGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGCCTg                        >  1:290465/1‑150 (MQ=255)
                                                                                                                          aaCGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGCCTg                        <  2:3731350/150‑1 (MQ=255)
                                                                                                                            cGGCCTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGCCTGGc                      >  1:5534723/1‑150 (MQ=255)
                                                                                                                                cTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGCCTGGCCGGc                  >  2:6328615/1‑150 (MQ=255)
                                                                                                                                cTGCTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGCCTGGCCGGc                  <  1:8164415/150‑1 (MQ=255)
                                                                                                                                   cTCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGCCTGGCCGGCGAc               >  1:1292173/1‑150 (MQ=255)
                                                                                                                                    tCCGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGCCTGGCCGGCGACg              <  1:8350922/150‑1 (MQ=255)
                                                                                                                                     ccGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCCGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGCCTGGCCGGCGACgt             >  1:8620818/1‑150 (MQ=255)
                                                                                                                                      cGTGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGCCTGGCCGGCGACgtg            >  2:10329145/1‑150 (MQ=255)
                                                                                                                                        tGGCCGCCGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGCCTGGCCGGCGACGTGtt          <  2:2162140/150‑1 (MQ=255)
                                                                                                                                          gccgccGTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGCCTGGCCGGCGACGTGTTcc        >  2:49407/1‑150 (MQ=255)
                                                                                                                                           ccgccgTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGCGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGCCTGGCCGGCGACGTGTTCCa       >  1:2173874/1‑150 (MQ=255)
                                                                                                                                              ccgTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGCCTGGCCGGCGACGTGTTCCAACt    >  1:3627603/1‑150 (MQ=255)
                                                                                                                                              ccgTGGCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGCCTGGCCGGCGACGTGTTCCAACt    >  2:905349/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TCCGCGCCGAGCCCTACGCCGGGCTGGAGCGCGAGCGCTTCGACGAAGTCCTGCGGATGCTCGCCGAGGGCTACCACTCACGCCTCGGCGTGCGCGGCGCCTATCTCCATCGCGATGCGCTCAACGGCCTGCTCCGTGGCCGCCGTGCCGCGCGGCTGACCGCGCTGACCTCGGGCGGGACCATTCCCGATACCGGCGACTACAGCGTGCTGCTGGAGCCGCAGGGCCTGCTGGTGGGAACGGTCAACGAGGACTTCGCGGTGGAGAGCCTGGCCGGCGACGTGTTCCAACTGG  >  NC_008463/1880725‑1881018

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑