Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 2,318,606 A→G E190E (GAA→GAG)  sbrI → ECF family RNA polymerase sigma factor SbrI

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084632,318,6060AG99.8% 1980.5 / NA 525E190E (GAA→GAG) sbrIECF family RNA polymerase sigma factor SbrI
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (260/264);  minor base C (1/0);  total (261/264)
Fisher's exact test for biased strand distribution p-value = 4.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 536 total aligned reads displayed.

GCCCGAGGAACAGCGCGAGGTATTCCTGATGCGCGCCCACGGAGAACTGGAACTGGCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAAGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCGCAGCAGCCGGCGCCGGCGCTGGACCAGGCGATCCTCGCCGCCGCGCGACGCCAGGCGGCGCAT  >  NC_008463/2318459‑2318752
                                                                                                                                                   |                                                                                                                                                  
gCCCGAGGAACAGCGCGAGGTATTCCTGATGCGCGCCCACGGAGAACTGGAACTGGCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAgga                                                                                                                                                  <  2:3593636/150‑1 (MQ=255)
gCCCGAGGAACAGCGCGAGGTATTCCTGATGCGCGCCCACGGAGAACTGGAACTGGCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAgga                                                                                                                                                  >  1:5288502/1‑150 (MQ=255)
  ccGAGGAACAGCGCGAGGTATTCCTGATGCGCGCCCACGGAGAACTGGAACTGGCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGaggagg                                                                                                                                                >  2:37989/1‑150 (MQ=255)
   cGAGGAACAGCGCGAGGTATTCCTGATGCGCGCCCACGGAGAACTGGAACTGGCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGt                                                                                                                                               <  2:6312769/150‑1 (MQ=255)
      ggAACAGCGCGAGGTATTCCTGATGCGCGCCCACGGAGAACTGGAACTGGCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGc                                                                                                                                            <  2:3330307/150‑1 (MQ=255)
       gAACAGCGCGAGGTATTCCTGATGCGCGCCCACGGAGAACTGGAACTGGCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCg                                                                                                                                           <  1:2432604/150‑1 (MQ=255)
        aaCAGCGCGAGGTATTCCTGATGCGCGCCCACGGAGAACTGGAACTGGCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCgg                                                                                                                                          <  1:511276/150‑1 (MQ=255)
         aCAGCGCGAGGTATTCCTGATGCGCGCCCACGGAGAACTGGAACTGGCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGt                                                                                                                                         >  2:9885305/1‑150 (MQ=255)
            gcgcGAGGTATTCCTGATGCGCGCCCACGGAGAACTGGAACTGGCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATg                                                                                                                                      <  1:4391422/150‑1 (MQ=255)
            gcgcGAGGTATTCCTGATGCGCGCCCACGGAGAACTGGAACTGGCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATg                                                                                                                                      <  2:6825475/150‑1 (MQ=255)
            gcgcGAGGTATTCCTGATGCGCGCCCACGGAGAACTGGAACTGGCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATg                                                                                                                                      <  1:4990922/150‑1 (MQ=255)
                gAGGTATTCCTGATGCGCGCCCACGGAGAACTGGAACTGGCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAAcc                                                                                                                                  >  1:5599199/1‑150 (MQ=255)
                gAGGTATTCCTGATGCGCGCCCACGGAGAACTGGAACTGGCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAAcc                                                                                                                                  <  2:9316520/150‑1 (MQ=255)
                 aGGTATTCCTGATGCGCGCCCACGGAGAACTGGAACTGGCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAAccc                                                                                                                                 >  1:2208477/1‑150 (MQ=255)
                 aGGTATTCCTGATGCGCGCCCACGGAGAACTGGAACTGGCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAAccc                                                                                                                                 <  1:10516704/150‑1 (MQ=255)
                  ggTATTCCTGATGCGCGCCCACGGAGAACTGGAACTGGCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGCGGTGGCGGTATGAACCca                                                                                                                                >  2:4354853/1‑150 (MQ=255)
                    tATTCCTGATGCGCGCCCACGGAGAACTGGAACTGGCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCcaca                                                                                                                              <  2:6394674/150‑1 (MQ=255)
                      ttCCTGATGCGCGCCCACGGAGAACTGGAACTGGCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGa                                                                                                                            <  1:5391311/150‑1 (MQ=255)
                       tCCTGATGCGCGCCCACGGAGAACTGGAACTGGCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGaa                                                                                                                           <  2:2383137/150‑1 (MQ=255)
                       tCCTGATGCGCGCCCACGGAGAACTGGAACTGGCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGaa                                                                                                                           <  2:4532668/150‑1 (MQ=255)
                        ccTGATGCGCGCCCACGGAGAACTGGAACTGGCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAg                                                                                                                          <  1:8811269/150‑1 (MQ=255)
                             tGCGCGCCCACGGAGAACTGGAACTGGCCGAGATCGCCACGCTCACCCCGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTc                                                                                                                     <  1:7843100/150‑1 (MQ=255)
                              gcgcgcCCACGGAGAACTGGAACTGGCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTcc                                                                                                                    <  2:1885323/150‑1 (MQ=255)
                               cgcgcCCACGGAGAACTGGAACTGGCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCcg                                                                                                                   <  1:3424484/150‑1 (MQ=255)
                                 cgcCCACGGAGAACTGGAACTGGCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCcgcg                                                                                                                 >  2:843075/1‑150 (MQ=255)
                                  gcgcACGGTGAACTGGAACTGGCCGAGATCGCCTCGCTCACCCAGTCTCCGCTGGTGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGATGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACACACAGAAGCATTCCGCGa                                                                                                                <  2:706888/147‑1 (MQ=255)
                                   cccACGGAGAACTGGAACTGGCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAg                                                                                                               <  2:1450567/150‑1 (MQ=255)
                                      aCGGAGAACTGGAACTGGCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGt                                                                                                            <  2:765148/150‑1 (MQ=255)
                                       cGGAGAACTGGAACTGGCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTc                                                                                                           <  1:695412/150‑1 (MQ=255)
                                         gagaACTGGAACTGGCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGATGAGCCGTGTCCGCGACGCGGTGCAGAAGCGGCGCCGGCTGCTGGCGGAGGAGGGGGCGGTATGAACCCAGAGTAGCGGTCCGCGAGCGTCGc                                                                                                         >  2:5675066/1‑150 (MQ=255)
                                         gagaACTGGAACTGGCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGc                                                                                                         >  1:4004059/1‑150 (MQ=255)
                                            aactggaactggCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGa                                                                                                      >  1:4805662/1‑150 (MQ=255)
                                            aactggaactggCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGGATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGa                                                                                                      >  1:5958937/1‑150 (MQ=255)
                                              ctggaactggCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAag                                                                                                    <  2:1654232/150‑1 (MQ=255)
                                                ggaactggCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAagag                                                                                                  >  1:10023456/1‑150 (MQ=255)
                                                 gaactggCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCCGAAGCTGCGCCGGCTGCTGGGCGAGGAGGTGGCGGGATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGACGAGc                                                                                                 >  1:2598178/1‑150 (MQ=255)
                                                  aactggCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGTGGGGGCGGTCTGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCa                                                                                                >  2:8069320/1‑150 (MQ=255)
                                                   actggCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAg                                                                                               <  2:11050210/150‑1 (MQ=255)
                                                     tggCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGAt                                                                                             <  1:10426285/150‑1 (MQ=255)
                                                     tggCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGAt                                                                                             <  1:474924/150‑1 (MQ=255)
                                                        ccGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCt                                                                                          >  1:3225964/1‑150 (MQ=255)
                                                        ccGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCAGCTGGCCGAGGAGGGGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCt                                                                                          >  2:4127474/1‑150 (MQ=255)
                                                          gagaTCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTgg                                                                                        >  2:10407346/1‑150 (MQ=255)
                                                           tgaTCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGTAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTGCGCGAGCGTCGCCGAAGAGCAGATGCTGgc                                                                                       >  1:6118864/2‑150 (MQ=255)
                                                           agaTCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGTGCAGATGCTGgc                                                                                       >  1:10978016/1‑150 (MQ=255)
                                                           agaTCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGgc                                                                                       <  1:3773556/150‑1 (MQ=255)
                                                            gaTCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGgcg                                                                                      >  1:284726/1‑150 (MQ=255)
                                                              tCGCCACGCTCACCCAGTCGCCGCGGGAGACGGTGAAGAGCCGTGTCCGCTACGCGGTGCACTAGAGGCGCCGGCGGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGCGCGTCGGCGCAGAGCAGATGCGGGCGCa                                                                                    >  1:2041477/1‑150 (MQ=255)
                                                                 ccACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGTGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCAttt                                                                                 >  1:5847289/1‑150 (MQ=255)
                                                                 ccACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGTAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCCttt                                                                                 >  1:1793057/1‑150 (MQ=255)
                                                                 ccACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCAttt                                                                                 >  2:4948584/1‑150 (MQ=255)
                                                                   aCGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTcc                                                                               >  2:2081379/1‑150 (MQ=255)
                                                                   aCGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTcc                                                                               <  1:1328037/150‑1 (MQ=255)
                                                                    cGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCcg                                                                              >  2:4594850/1‑150 (MQ=255)
                                                                    cGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGGTTGAACCCACAGAAGCATTCCGCGAGCGTCGCCGTAGAGCAGATGCTGGCGCATTTCcg                                                                              >  1:1473849/1‑150 (MQ=255)
                                                                        cACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGcc                                                                          <  1:9386335/150‑1 (MQ=255)
                                                                         aCCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGTAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGccc                                                                         <  2:325063/150‑1 (MQ=255)
                                                                         aCCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGccc                                                                         >  1:6863972/1‑150 (MQ=255)
                                                                         aCCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTGTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACCGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGccc                                                                         >  2:54659/1‑150 (MQ=255)
                                                                           ccAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCAc                                                                       <  1:1982225/150‑1 (MQ=255)
                                                                           ccAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCAc                                                                       <  1:8239260/150‑1 (MQ=255)
                                                                             aGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCAcgc                                                                     >  1:6399425/1‑150 (MQ=255)
                                                                                cgccgcTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGcgcc                                                                  <  1:9089255/150‑1 (MQ=255)
                                                                                cgccgcTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGcgcc                                                                  <  2:8770745/150‑1 (MQ=255)
                                                                                  ccgcTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGcgccgc                                                                <  2:893053/150‑1 (MQ=255)
                                                                                   cgcTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCgca                                                               <  2:6020122/150‑1 (MQ=255)
                                                                                    gcTGGAGCCGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCgcag                                                              <  1:10711308/150‑1 (MQ=255)
                                                                                    gcTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCgcag                                                              <  1:8361099/150‑1 (MQ=255)
                                                                                      tGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACCGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCgccgca                                                            >  1:6752833/1‑150 (MQ=255)
                                                                                      tGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCgcagca                                                            >  2:2862885/1‑150 (MQ=255)
                                                                                       ggAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCGcagcag                                                           >  2:9573621/1‑150 (MQ=255)
                                                                                       ggAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCGcagcag                                                           <  2:1360818/150‑1 (MQ=255)
                                                                                        gtgaCGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCGCagcagc                                                          <  2:670683/148‑1 (MQ=255)
                                                                                        gagaCGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCGCagcagc                                                          <  2:10843663/150‑1 (MQ=255)
                                                                                              gTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGGGGTGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGGCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCCCGCGCCGCGGCAgccggcgc                                                    >  2:5045923/1‑150 (MQ=255)
                                                                                               tGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGCGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCGCAGCAgccggcgcc                                                   >  2:9405744/1‑150 (MQ=255)
                                                                                                  agagCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCGCAGCAgccggcgccggc                                                <  1:713328/150‑1 (MQ=255)
                                                                                                     gCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCGCAGCAGCCGGCGCCGGCGCt                                             <  2:7835047/150‑1 (MQ=255)
                                                                                                      ccGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCGCAGCAGCCGGCGCCGGCGCTg                                            >  1:1192300/1‑150 (MQ=255)
                                                                                                      ccGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCGCAGCAGCCGGCGCCGGCGCTg                                            >  1:10795339/1‑150 (MQ=255)
                                                                                                       cGTGTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCGCAGCAGCCGGCGCCGGCGCTgg                                           <  2:10600517/150‑1 (MQ=255)
                                                                                                         tttCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCGCAGCAGCCGGCGCCGGCGCTGGAc                                         >  2:6093926/1‑150 (MQ=255)
                                                                                                         tttCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCGCAGCAGCCGGCGCCGGCGCTGGAc                                         >  2:3051126/1‑150 (MQ=255)
                                                                                                                tACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCGCAGCAGCCGGCGCCGGCGCTGGACCAGGCGa                                  >  1:7734110/1‑150 (MQ=255)
                                                                                                                tACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCGCAGCAGCCGGCGCCGGCGCTGGACCAGGCGa                                  <  2:5245828/150‑1 (MQ=255)
                                                                                                                 aCGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGACGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCGCAGCAGCCGGCGCCGGCGCTGGACCAGGCGAt                                 <  1:4666882/150‑1 (MQ=255)
                                                                                                                 aCGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCGCAGCAGCCGGCGCCGGCGCTGGACCAGGCGAt                                 >  1:9721047/1‑150 (MQ=255)
                                                                                                                    cgGTGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCGCAGCAGCCGGCGCCGGCGCTGGACCAGGCGATCCt                              >  1:9515549/1‑150 (MQ=255)
                                                                                                                    cgGGGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCGCAGCAGCCGGCGCCGGCGCTGGACCAGGCGATCCt                              <  2:126230/150‑1 (MQ=255)
                                                                                                                       tGCAGAAGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCGCAGCAGCCGGCGCCGGCGCTGGACCAGGCGATCCTcgc                           >  2:6187645/1‑150 (MQ=255)
                                                                                                                            aaGCTGCGCCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCGCAGCAGCCGGCGCCGGCGCTGGACCAGGCGATCCTcgccgccg                      >  1:3029149/1‑150 (MQ=255)
                                                                                                                                  cgcCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCGCAGCAGCCGGCGCCGGCGCTGGACCAGGCGATCCTCGCCGCCGCGCGAc                <  2:5851433/150‑1 (MQ=255)
                                                                                                                                   gcCGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCGCAGCAGCCGGCGCCGGCGCTGGACCAGGCGATCCTCGCCGCCGCGCGACg               >  1:7543436/1‑150 (MQ=255)
                                                                                                                                     cGGCTGCTGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCGCAGCAGCCGGCGCCGGCGCTGGACCAGGCGATCCTCGCCGCCGCGCGACGcc             >  2:7460501/1‑150 (MQ=255)
                                                                                                                                       gctgctGGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCGCAGCAGCCGGCGCCGGCGCTGGACCAGGCGATCCTCGCCGCCGCGCGACGCCAg           >  2:8570091/1‑150 (MQ=255)
                                                                                                                                        ctgctgGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCGCAGCAGCCGGCGCCGGCGCTGGACCAGGCGATCCTCGCCGCCGCGCGACGCCAgg          >  2:2158889/1‑150 (MQ=255)
                                                                                                                                          gctgGCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCGCAGCAGCCGGCGCCGGCGCTGGACCAGGCGATCCTCGCCGCCGCGCGACGCCAggcg        >  2:7277082/1‑150 (MQ=255)
                                                                                                                                             ggCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCGCAGCAGCCGGCGCCGGCGCTGGACCAGGCGATCCTCGCCGCCGCGCGACGCCAGgcggcg     <  2:1009320/150‑1 (MQ=255)
                                                                                                                                              gCCGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCGCAGCAGCCGGCGCCGGCGCTGGACCAGGCGATCCTCGCCGCCGCGCGACGCCAGGCGgcgc    >  1:419981/1‑150 (MQ=255)
                                                                                                                                                cGAGGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCGCAGCAGCCGGCGCCGGCGCTGGACCAGGCGATCCTCGCCGCCGCGCGACGCCAGGCGGCGCAt  >  1:8502100/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GCCCGAGGAACAGCGCGAGGTATTCCTGATGCGCGCCCACGGAGAACTGGAACTGGCCGAGATCGCCACGCTCACCCAGTCGCCGCTGGAGACGGTGAAGAGCCGTTTCCGCTACGCGGTGCAGAAGCTGCGCCGGCTGCTGGCCGAAGAGGTGGCGGTATGAACCCACAGAAGCATTCCGCGAGCGTCGCCGAAGAGCAGATGCTGGCGCATTTCCGCGCCCACGCGCCGCAGCAGCCGGCGCCGGCGCTGGACCAGGCGATCCTCGCCGCCGCGCGACGCCAGGCGGCGCAT  >  NC_008463/2318459‑2318752

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑