Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 2,407,463 A→G K125E (AAG→GAG)  PA14_RS11255 → glutathione S‑transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084632,407,4630AG99.8% 1981.1 / NA 511K125E (AAG→GAG) PA14_RS11255glutathione S‑transferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (236/274);  minor base C (0/1);  total (236/275)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 511 total aligned reads displayed.

CGCTATGCGCCGGGCGACCTCTATCCGCAGGACCCGGTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACCCTGCGTACGCCGCCGGAGCAACGCGACAAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTGACATTCCCCTGGGCTGTTTCATCTACGCCTGGTTCGAG  >  NC_008463/2407316‑2407609
                                                                                                                                                   |                                                                                                                                                  
cgcTATGCGCCGGGCGACCTCTATCCGCAGGACCCGGTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAg                                                                                                                                                  <  2:5108846/150‑1 (MQ=255)
 gcTATGCGCCGGGCGACCTCTATCCGCAGGACCCGGTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAgg                                                                                                                                                 <  2:2691752/150‑1 (MQ=255)
  cTATGCGCCGGGCGACCTCTATCCGCAGGACCCGGTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGc                                                                                                                                                >  1:7366504/1‑150 (MQ=255)
   tctGCGCCGGGCGACCTCTATCCGCAGGACCCGGTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGcc                                                                                                                                               <  2:8479829/148‑1 (MQ=255)
      gcgcCGGGCGACCTCTATCCGCAGGACCCGGTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTg                                                                                                                                            <  1:7825750/150‑1 (MQ=255)
        gcCGGGCGACCTCTATCCGCAGGACCCGGTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGAt                                                                                                                                          >  2:868156/1‑150 (MQ=255)
            ggCGACCTCTATCCGCAGGACCCGGTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCa                                                                                                                                      <  2:10489524/150‑1 (MQ=255)
             gCGACCTCTATCCGCAGGACCCGGTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCaa                                                                                                                                     <  2:7405603/150‑1 (MQ=255)
                  ctctATCCGCAGGACCCGGTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGgcg                                                                                                                                >  2:6198636/1‑150 (MQ=255)
                   tctATCCGCAGGACCCGGTGCGCCGCGCCGACGCCGACACGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGgcgc                                                                                                                               >  1:9636801/1‑150 (MQ=255)
                    ctATCCGCAGGACCCGGTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCt                                                                                                                              <  2:9410543/150‑1 (MQ=255)
                    ctATCCGCAGGACCCGGTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCt                                                                                                                              <  2:9291561/150‑1 (MQ=255)
                     tATCCGCAGGACCCGGTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTg                                                                                                                             >  2:3414545/1‑150 (MQ=255)
                      aTCCGCAGGACCCGGTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGc                                                                                                                            <  1:600440/150‑1 (MQ=255)
                      aTCCGCAGGACCCGGTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGc                                                                                                                            >  1:2373991/1‑150 (MQ=255)
                        ccGCAGGACCCGGTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGGTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAg                                                                                                                          <  1:4093828/150‑1 (MQ=255)
                           cAGGACCCGGTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAACGGCGCTGCAGAcc                                                                                                                       <  2:5664209/150‑1 (MQ=255)
                            aGGACCCGGTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCt                                                                                                                      <  1:6240664/150‑1 (MQ=255)
                               aCCCGGTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTgcg                                                                                                                   <  2:9113069/150‑1 (MQ=255)
                               aCCCGGTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTgcg                                                                                                                   >  1:748457/1‑150 (MQ=255)
                               aCCCGGTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTgcg                                                                                                                   <  2:9768030/150‑1 (MQ=255)
                                cccGGTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGGTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTgcgg                                                                                                                  <  2:7962771/150‑1 (MQ=255)
                                  cGGTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTgcggcg                                                                                                                >  1:1647202/1‑150 (MQ=255)
                                   ggTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGa                                                                                                               <  2:1209215/150‑1 (MQ=255)
                                    gTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAg                                                                                                              >  2:3706195/1‑150 (MQ=255)
                                      gcgccgcgccGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGtt                                                                                                            >  2:2851484/1‑150 (MQ=255)
                                         ccgcgccgACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTTCGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTtgct                                                                                                         <  2:6505711/150‑1 (MQ=255)
                                          cgcgccgACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTtgctg                                                                                                        <  1:102842/150‑1 (MQ=255)
                                              ccgacgccgacAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCggg                                                                                                    <  1:8251147/150‑1 (MQ=255)
                                                 acgccgacAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGc                                                                                                 <  1:8878668/150‑1 (MQ=255)
                                                  cgccgacAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGcc                                                                                                >  2:2095880/1‑150 (MQ=255)
                                                   gccgacAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCg                                                                                               <  2:3523407/150‑1 (MQ=255)
                                                    ccgacAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCgg                                                                                              <  1:2557927/150‑1 (MQ=255)
                                                     cgacAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGa                                                                                             <  2:4096776/150‑1 (MQ=255)
                                                     cgacAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGa                                                                                             >  1:897574/1‑150 (MQ=255)
                                                        cAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCa                                                                                          >  1:10721135/1‑150 (MQ=255)
                                                         aaGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAg                                                                                         <  1:330428/150‑1 (MQ=255)
                                                           gTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGAc                                                                                       >  1:7031823/1‑150 (MQ=255)
                                                           gTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGAc                                                                                       >  1:8530576/1‑150 (MQ=255)
                                                                  gactggacCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTggcc                                                                                >  1:6473015/1‑150 (MQ=255)
                                                                    ctggacCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTggccgg                                                                              >  2:5243430/1‑150 (MQ=255)
                                                                    ctggacCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTggccgg                                                                              >  1:4525938/1‑150 (MQ=255)
                                                                      ggacCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTggccggcc                                                                            >  1:800562/1‑150 (MQ=255)
                                                                      ggacCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTggccggcc                                                                            <  1:439984/150‑1 (MQ=255)
                                                                        accacaTCGACTCTCGCCGGGAAGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACATGCGGCGAGTTGCTGAGGGTGCCGGACCAGACCCTGGCCGGCCaa                                                                          >  2:1351961/1‑150 (MQ=255)
                                                                         ccaccTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCCACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAAc                                                                         <  2:5868874/150‑1 (MQ=255)
                                                                           accTCGACTCTCGCCGGGCCGTTCCGCGACCTGGTCTGGGGATCGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGTCCTGCGGCGAGTGGCTGCGGGAGCCGGACCAGACCCTGGCCGGCCAAccc                                                                       <  1:10472293/150‑1 (MQ=255)
                                                                            ccTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCt                                                                      >  2:6251560/1‑150 (MQ=255)
                                                                            ccTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCt                                                                      <  1:9978251/150‑1 (MQ=255)
                                                                              tCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTgg                                                                    >  2:2942369/1‑150 (MQ=255)
                                                                                  ctctCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGt                                                                <  2:4758888/150‑1 (MQ=255)
                                                                                   tctcGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTc                                                               <  1:9226722/150‑1 (MQ=255)
                                                                                    ctcGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTcc                                                              >  2:4306986/1‑150 (MQ=255)
                                                                                      cGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCgg                                                            <  2:10192412/150‑1 (MQ=255)
                                                                                       gccgggccggTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGc                                                           <  2:4905164/150‑1 (MQ=255)
                                                                                       gCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGc                                                           >  1:2709157/1‑150 (MQ=255)
                                                                                       gCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGc                                                           <  2:8040725/150‑1 (MQ=255)
                                                                                        ccGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCg                                                          <  2:2266651/150‑1 (MQ=255)
                                                                                        ccGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCg                                                          >  2:6944572/1‑150 (MQ=255)
                                                                                        ccGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCg                                                          >  1:367850/1‑150 (MQ=255)
                                                                                         cGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGa                                                         <  1:5411186/150‑1 (MQ=255)
                                                                                          gggCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGaa                                                        >  1:5097010/1‑150 (MQ=255)
                                                                                          gggCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGaa                                                        >  2:32955/1‑150 (MQ=255)
                                                                                            gCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACg                                                      >  2:1511768/1‑150 (MQ=255)
                                                                                             ccGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGc                                                     >  2:1931052/1‑150 (MQ=255)
                                                                                             ccGTTCCGCGACCTGGTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGc                                                     <  2:4485488/150‑1 (MQ=255)
                                                                                               gTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGTGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCtt                                                   >  2:10998869/1‑150 (MQ=255)
                                                                                                ttCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTc                                                  >  1:313128/1‑150 (MQ=255)
                                                                                                   cgcgACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGc                                               <  1:3930011/150‑1 (MQ=255)
                                                                                                   cgcgACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGc                                               <  1:10856329/150‑1 (MQ=255)
                                                                                                   cgcgACCTGTTCTGGGGAACGCTGCGGACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAACGGCGCTGCAGACCAGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGc                                               <  2:3214857/150‑1 (MQ=255)
                                                                                                       aCCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATgg                                           >  2:3935168/1‑150 (MQ=255)
                                                                                                       aCCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATgg                                           >  2:5560701/1‑150 (MQ=255)
                                                                                                         cTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGt                                         <  2:4635807/150‑1 (MQ=255)
                                                                                                             tCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTGACa                                     >  2:7612594/1‑150 (MQ=255)
                                                                                                                ggggAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTGACATTc                                  <  2:2537991/150‑1 (MQ=255)
                                                                                                                ggggAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTGACATTc                                  <  1:10621348/150‑1 (MQ=255)
                                                                                                                   gAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTGACATTcccc                               <  1:2067378/150‑1 (MQ=255)
                                                                                                                    aaCGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTGACATTCCCCt                              <  1:188642/150‑1 (MQ=255)
                                                                                                                     acgcTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTGACATTCCCCTg                             <  2:6785501/147‑1 (MQ=255)
                                                                                                                      cgcTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTGACATTCCCCTgg                            <  2:715626/148‑1 (MQ=255)
                                                                                                                       gcTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTGACATTCCCCTggg                           <  1:6122547/149‑1 (MQ=255)
                                                                                                                        cTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTGACATTCCCCTGGGc                          >  1:3031846/1‑150 (MQ=255)
                                                                                                                         tGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTGACATTCCCCTGGGcg                         >  2:5781571/1‑149 (MQ=255)
                                                                                                                         tGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTGACATTCCCCTGGGCt                         >  1:6918209/1‑150 (MQ=255)
                                                                                                                          gCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTGACATTCCCCTGGGCTg                        >  1:1451766/1‑150 (MQ=255)
                                                                                                                            gTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTGACATTCCCCTGGGCTGtt                      >  2:9630549/1‑150 (MQ=255)
                                                                                                                              aCGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTGACATTCCCCTGGGCTGTTTc                    <  2:9446507/150‑1 (MQ=255)
                                                                                                                                gccgccGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTGACATTCCCCTGGGCTGTTTCAt                  >  1:6720921/1‑150 (MQ=255)
                                                                                                                                 ccgccgGAGCAACGCGACGAGGCCCTGATAGCAAAGGCGCTGCAGACCTGCGGCGAGTTTCTGCGGGTGCCGGACCAGACCCTGGCCGGCCATCCATGGCTGTCCGGCGAACGCTTCGGCATGGGTGACATTCCCATGGGCTGTTTCATc                 <  1:10059142/150‑1 (MQ=255)
                                                                                                                                  cgccgGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTGACATTCCCCTGGGCTGTTTCATCt                >  1:4817921/1‑150 (MQ=255)
                                                                                                                                   gccgGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTGACATTCCCCTGGGCTGTTTCATCTa               >  1:7233739/1‑150 (MQ=255)
                                                                                                                                   gccgGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGGGACATTCCCCTGGGCTGTTTCATCTa               >  2:10854639/1‑150 (MQ=255)
                                                                                                                                       gAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTGACATTCCCCTGGGCTGTTTCATCTAAGcc           >  2:5324103/1‑150 (MQ=255)
                                                                                                                                        aGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTGACATTCCCCTGGGCTGTTTCATCTACGCCt          >  1:4251046/1‑150 (MQ=255)
                                                                                                                                          cAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTGACATTCCCCTGGGCTGTTTCATCTACGCCTgg        >  1:11055636/1‑150 (MQ=255)
                                                                                                                                           aaCGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTGACATTCCCCTGGGCTGTTTCATCTACGCCTGGt       <  1:120164/150‑1 (MQ=255)
                                                                                                                                              gcgACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTGACATTCCCCTGGGCTGTTTCATCTACGCCTGGTTCg    >  2:8801036/1‑150 (MQ=255)
                                                                                                                                              gcgACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTGACATTCCCCTGGGCTGTTTCATCTACGCCTGGTTCg    >  1:5815187/1‑150 (MQ=255)
                                                                                                                                                gACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTGACATTCCCCTGGGCTGTTTCATCTACGCCTGGTTCgag  >  2:8309487/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CGCTATGCGCCGGGCGACCTCTATCCGCAGGACCCGGTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACCCTGCGTACGCCGCCGGAGCAACGCGACAAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTGACATTCCCCTGGGCTGTTTCATCTACGCCTGGTTCGAG  >  NC_008463/2407316‑2407609

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑