Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 2,707,727 N→G ?75P (CNT→CCT)  PA14_RS12740 ← DUF2285 domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084632,707,7270NG99.4% 1369.3 / NA 358?75P (CNT→CCT) PA14_RS12740DUF2285 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base N (0/0);  major base G (204/152);  minor base T (0/2);  total (204/154)
Fisher's exact test for biased strand distribution p-value = 1.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 362 total aligned reads displayed.

GCAGCAGCCGGGCCAATGCGACACCAGCACCAGGCGCTTGCCGTCGTGGATCAGTTGCTTGCGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGCATCGTGGTCANGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCTGGGTCTTCCAGCAGGCGCAGGCTCCAAGCCTGCGCCGCGTCCGGCCAGCGGCGACGGCGCAGCCAGTCGCGGCGGTAATCGGGATTCCTGCGCAGGTATTCCCAAGCCA  >  NC_008463/2707579‑2707874
                                                                                                                                                    |                                                                                                                                                   
gcagcaGCCGGGCCAATGCGACACCAGCACCAGGCGCTTGCCGTCGTGGATCAGTTGCTTGCGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGCATCGTGGTCAgg                                                                                                                                                    >  1:8999476/1‑150 (MQ=255)
   gcagcCGGGCCAATGCGACACCAGCACCAGGCGCTTGCCGTCGTGGATCAGTTGCTTGCGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGCATCGTGGTCAGGGaa                                                                                                                                                 >  1:1716750/1‑150 (MQ=255)
     agcCGGGCCAATGCGACACCAGCACCAGGCGCTTGCCGTCGTGGATCAGTTGCTTGCGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGCATCGTGGTCAGGGAccc                                                                                                                                               >  1:7092022/1‑150 (MQ=255)
     agcCGGGCCAATGCGACACCAGCACCAGGCGCTTGCCGTCGTGGATCAGTTGCTTGCGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGCATCGTGGTCAGGGAAcc                                                                                                                                               <  2:1713292/150‑1 (MQ=255)
      gcCGGGCCAATGCGACACCAGCACCAGGCGCTTGCCGTCGTGGATCAGTTGCTTGCGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCTCGGCTTCGTGGTCAGGGAACCa                                                                                                                                              >  2:5477560/1‑150 (MQ=255)
       ccGGGCCAATGCGACACCAGCACCAGGCGCTTGCCGTCGTGGATCAGTTGCTTGCGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGCATCGTGGTCAGGGAACCAg                                                                                                                                             <  1:7559085/150‑1 (MQ=255)
       ccGGGCCAATGCGACACCAGCACCAGGCGCTTGCCGTCGTGGATCAGTTGCTTGCGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGCATCGTGGTCAGGGAACCAg                                                                                                                                             <  2:370064/150‑1 (MQ=255)
        cGGGCCAATGCGACACCAGCACCAGGCGCTTGCCGTCGTGGATCAGTTGCTTGCGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGCATCGTGGTCAGGGAACCAgg                                                                                                                                            <  1:10724942/150‑1 (MQ=255)
         gggCCAATGCGACACCAGCACCAGGCGCTTGCCGTCGTGGATCAGTTGCTTGCGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGCATCGTGGTCAGGGAACCAGGc                                                                                                                                           <  1:6125134/150‑1 (MQ=255)
         gggCCAATGCGACACCAGCACCAGGCGCTTGCCGTCGTGGATCAGTTGCTTGCGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGCATCGTGGTCAGGGAACCAGGc                                                                                                                                           >  1:1419386/1‑150 (MQ=255)
          ggCCAATGCGACACCAGCACCAGGCGCTTGCCGTCGTGGATCAGTTGCTTGCGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGCATCGTGGTCAGGGAACCAGGcc                                                                                                                                          >  1:791155/1‑150 (MQ=255)
           gCCAATGCGACACCAGCACCAGGCGCTTGCCGTCGTGGATCAGTTGCTTGCGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGCATCGTGGTCAGGGAACCAGGCCg                                                                                                                                         >  1:10177258/1‑150 (MQ=255)
            ccAATGCGACACCAGCACCAGGCGCTTGCCGTCGTGGATCAGTTGCTTGCGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGCATCGTGGTCAGGGAACCAGGCCgg                                                                                                                                        <  2:2435661/150‑1 (MQ=255)
            ccAATGCGACACCAGCACCAGGCGCTTGCCGTCGTGGATCAGTTGCTTGCGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGCATCGTGGTCAGGGAACCAGGCCgg                                                                                                                                        >  1:4586745/1‑150 (MQ=255)
             cAATGCGACACCAGCACCAGGCGCTTGCCGTCGTGGATCAGTTGCTTGCGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGCATCGTGGTCAGGGAACCAGGCCGGa                                                                                                                                       >  1:4183200/1‑150 (MQ=255)
               aTGCGACACCAGCACCAGGCGCTTGCCGTCGTGGATCAGTTGCTTGCGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGCATCGTGGTCAGGGACCCAGGCCGGATg                                                                                                                                     >  2:4185727/1‑150 (MQ=255)
               aTGCGACACCAGCACCAGGCGCTTGCCGTCGTGGATCAGTTGCTTGCGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGCATCGTGGTCAGGGAACCAGGCCGGacg                                                                                                                                     <  1:3801738/150‑3 (MQ=255)
                 gCGACACCAGCACCAGGCGCTTGCCGTCGTGGATCAGTTGCTTGCGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGCATCGTGGTCAGGGAACCAGGCCGGATgcg                                                                                                                                   <  2:3396409/150‑1 (MQ=255)
                 gCGACACCAGCACCAGGCGCTTGCCGTCGTGGATCAGTTGCTTGCGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGCATCGTGGTCAGGGAACCAGGCCGGATgcg                                                                                                                                   >  2:3268110/1‑150 (MQ=255)
                  cGACACCAGCACCAGGCGCTTGCCGTCGTGGATCAGTTGCTTGCGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGCATCGTGGTCAGGGAACCAGGCCGGATgcgc                                                                                                                                  <  2:2860339/150‑1 (MQ=255)
                   gACACCAGCACCAGGCGCTTGCCGTCGTGGATCAGTTGCTTGCGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGCATCGTGGTCAGGGAACCAGGCCGGATGcgcg                                                                                                                                 <  2:4747399/150‑1 (MQ=255)
                    acacCAGCACCAGGCGCTTGCCGTCGTGGATCAGTTGCTTGCGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGCATCGTGGTCAGGGAACCAGGCCGGATGCGCGt                                                                                                                                <  1:2407993/150‑1 (MQ=255)
                      accagcaccagGCGCTTGCCGTCGTGGATCAGTTGCTTGCGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGCATCGTGGTCAGGGAACCAGGCCGGATGCGCGTcg                                                                                                                              <  1:8315817/150‑1 (MQ=255)
                       ccagcaccagGCGCTTGCCGTCGTGGATCAGTTGCTTGCGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGCATCGTGGTCAGGGAACCAGGCCGGATGCGCGTcgc                                                                                                                             <  1:6727247/150‑1 (MQ=255)
                           caccagGCGCTTGCCGTCGTGGATCAGTTGCTTGCGGCCGGGAACGCGCCAGAACTCGATGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGCATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCgcgcgc                                                                                                                         <  1:5978786/150‑1 (MQ=255)
                             ccagGCGCTTGCCGTCGTGGATCAGTTGCTTGCGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTNCACCACGGCATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCAt                                                                                                                       <  1:5783046/150‑1 (MQ=255)
                             ccagGCGCTTGCCGTCGTGGATCAGTTGCTTGCGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGCATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCAt                                                                                                                       >  1:9872460/1‑150 (MQ=255)
                             ccagGCGCTTGCCGTCGTGGATCAGTTGCTTGCGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGCATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCAt                                                                                                                       <  1:7439282/150‑1 (MQ=255)
                               agGCGCTTGCCGTCGTGGATCAGTTGCTTGCGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGCATCGTGGTCAGGGAACCTGGCCGGATGCGCGTCGCGCGCATcc                                                                                                                     >  1:3582393/1‑150 (MQ=255)
                               agGCGCTTGCCGTCGTGGATCAGTTGCTTGCGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGCATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATcc                                                                                                                     <  2:7034264/150‑1 (MQ=255)
                                 gcgcTTGCCGTCGTGGATCAGTTGCTTGCGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGCATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCaa                                                                                                                   <  2:9785515/150‑1 (MQ=255)
                                    cTTGCCGTCGTGGATCAGTTGCTTGCGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGCATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGc                                                                                                                >  1:6431523/1‑150 (MQ=255)
                                     ttGCCGTCGTGGATCAGTTGCTTGCGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGCATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCt                                                                                                               >  2:5145809/1‑150 (MQ=255)
                                     ttGCCGTCGTGGATCAGTTGCTTGCGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGCATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCt                                                                                                               >  2:4607545/1‑150 (MQ=255)
                                     ttGCCGTCGTGGATCAGTTGCTTGCGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGCATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCt                                                                                                               >  1:3227805/1‑150 (MQ=255)
                                      tGCCGTCGTGGATCAGTTGCTTGCGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCGGCACCACGGCATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCTg                                                                                                              <  2:1882151/150‑1 (MQ=255)
                                       gCCGTCGTGGATCAGTTGCTTGCGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGCATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCTgg                                                                                                             >  2:10333014/1‑150 (MQ=255)
                                       gCCGTCGTGGATCAGTTGCTTGCGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGCATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCTgg                                                                                                             <  1:9998910/150‑1 (MQ=255)
                                       gCCGTCGTGGATCAGTTGCTTGCGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGCATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCTgg                                                                                                             >  1:4303199/1‑150 (MQ=255)
                                         cgtcgtGGATCAGTTGCTTGCGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGCATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCTgggg                                                                                                           >  2:6590513/1‑149 (MQ=255)
                                          gtcgtGGATCAGTTGCTTGCGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGCATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCTGGGTc                                                                                                          >  1:10027462/1‑150 (MQ=255)
                                          gtcgtGGATCAGTTGCTTGCGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGCATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCTGGGTc                                                                                                          >  2:5439/1‑150 (MQ=255)
                                          gtcgtGGATCAGTTGCATGCGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCTCCACGGCATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCTggggc                                                                                                          >  2:2500057/1‑148 (MQ=255)
                                           tcgtGGATCAGTTGCTTGCGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGCATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCTGGGTCt                                                                                                         <  1:6352916/150‑1 (MQ=255)
                                             gtGGATCAGTTGCTTGCGGCCGGGACCGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGCATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCTGGGTCTTc                                                                                                       >  1:5530058/1‑150 (MQ=255)
                                                  tCAGTTGCTTGCGGCCGGGAACGCGCCAGAACTCGAAGGCTTGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGCATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCTGGGTCTTCcagca                                                                                                  <  1:9804141/150‑1 (MQ=255)
                                                  tCAGTTGCTTGCGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGCATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCTGGGTCTTCcagca                                                                                                  <  2:5622836/150‑1 (MQ=255)
                                                  tCAGTTGCTTGCGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGCATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCTGGGTCTTCcagca                                                                                                  >  2:6014531/1‑150 (MQ=255)
                                                  tCAGTTGCTTGCGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGCATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCTGGGTCTTCcagca                                                                                                  <  2:1615080/150‑1 (MQ=255)
                                                   cAGTTGCTTGCGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATCGAGCTGCACCACGGCATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCTGGGTCTTCcagcag                                                                                                 <  1:10941468/150‑1 (MQ=255)
                                                    aGTTGCTTGCGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGCATCGTGGTCAGGGACCCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCTGGGTCTTCCAgcagg                                                                                                >  2:7467747/1‑150 (MQ=255)
                                                    aGTTGCTTGCGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGCATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCTGGGTCTTCCAgcagg                                                                                                >  2:5849832/1‑150 (MQ=255)
                                                     gTTGCTTGCGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGCATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCTGGGTCTTCCAgcaggc                                                                                               >  2:2176219/1‑150 (MQ=255)
                                                      ttgcttgcGGCCGGGAACGCGCCTGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGCATCGTGGTCAGGGAACCAGGCCGGAGGCGCGTCGCGCGCATCCAAGGCTGGGTCTTCCAgcaggcg                                                                                              <  2:4021017/150‑1 (MQ=255)
                                                      ttgcttgcGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGCATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCTGGGTCTTCCAgcaggcg                                                                                              >  2:6784345/1‑150 (MQ=255)
                                                       tgcttgcGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGCATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCTGGGTCTTCCAgcaggcgc                                                                                             <  2:6384170/150‑1 (MQ=255)
                                                       ggctggcGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGCATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCTGGGTCTTCCAgcaggcgc                                                                                             <  2:3099419/149‑1 (MQ=255)
                                                        gcttgcGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGCATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCTGGGTCTTCCAgcaggcgca                                                                                            <  2:7411301/150‑1 (MQ=255)
                                                          ttgcGGCCGGGACCGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGCATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCTGGGTCTTCCAgcaggcgcagg                                                                                          >  2:7728814/1‑150 (MQ=255)
                                                             cGGCCGGGACCGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGCACCGTGGTCAGGGAACCTGGCCGGATGCGCGTCGCGCGCATCCAAGGCTGGGTCTTCCAGCAGGCGCAGGCTc                                                                                       <  1:4645639/150‑1 (MQ=255)
                                                              ggCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGCATCGTGGTCAGGGACCCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCTGGGTCTTCCAGCAGGCGCAGGCTcc                                                                                      <  1:3478098/150‑1 (MQ=255)
                                                              ggCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGCATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCTGGGTCTTCCAGCAGGCGCAGGCTcc                                                                                      >  1:2180857/1‑150 (MQ=255)
                                                                ccGGGACCGCGCCAGAACTCGCAGGCATGGGCGTCGGGTGGCGGGTCGGCGTCGGGATAGAGCGGCACCACGGCATCGTGGTCAGGGAACCAGGCCGGCTGCGCGTCGCGCGCATCCAAGGCTGGGTCTTCCAGCAGGCGCAGGCTCCaa                                                                                    <  2:7817933/150‑1 (MQ=255)
                                                                ccGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGTTGGTGCTGCTCCACGGCATGGTGGGCTGGGACCCGGGCCGGATCCGCGTCGCGCGCATCCATGGGGGGGTCTGCCTGCAGGCGCTGGCTCCag                                                                                    >  1:9660023/1‑149 (MQ=255)
                                                                ccGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGCATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCTGGGTCTTCCAGCAGGCGCAGGCTCCaa                                                                                    >  1:4512818/1‑150 (MQ=255)
                                                                ccGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGCATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCTGGGTCTTCCAGCAGGCGCAGGCTCCaa                                                                                    >  1:10467774/1‑150 (MQ=255)
                                                                  gggAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGCATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCTGGGTCTTCCAGCAGGCGCAGGCTCCAAGc                                                                                  <  2:1292953/150‑1 (MQ=255)
                                                                    gaccgcgCCAGAACTCCACGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGCATCGTGCTCAGGGACCGCGGCCGTTTGCGCGTCGCGCGCATCCAAGGCTGGGTCTTCCAGCAGGCGCAGGCTCCAAGCCt                                                                                <  1:5046330/147‑1 (MQ=255)
                                                                      aCGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGCATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCTGGGTCTTCCAGCAGGCGCAGGCTCCAAGCCTgc                                                                              >  1:1276672/1‑150 (MQ=255)
                                                                       cgcgCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGCATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCTGGGTCTTCCAGCAGGCGCAGGCTCCAAGCCTgcg                                                                             <  2:5689043/150‑1 (MQ=255)
                                                                       cgcgCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCGGCACCACGGCATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCTGGGTCTTCCAGCAGGCGCAGGCTCCAAGCCTgcg                                                                             <  2:1061436/150‑1 (MQ=255)
                                                                            cAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGCATCGTGGTCAGGGAACCTGGCCGGATGCGCGTCGCGCGCATCCAAGGCTGGGTCTTCCAGCAGGCGCAGGCTCCAAGCCTGCGCcgcg                                                                        >  1:5251339/1‑150 (MQ=255)
                                                                             aGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGCATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCTGGGTCTTCCAGCAGGCGCAGGCTCCAAGCCTGCGCCGCGt                                                                       >  1:4745837/1‑150 (MQ=255)
                                                                              gAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGCATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCTGGGTCTTCCAGCAGGCGCAGGCTCCAAGCCTGCGCCGCGTc                                                                      >  1:1880654/1‑150 (MQ=255)
                                                                               aaCTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGCATCGTGGTCAGGGACCCGGGCCGGATGCGCGTCGCGCGCATCCAAGGCTGGGTCTTCCAGCAGGCGCAGGCTCCGAGCCTGCGCCGCGGcc                                                                     >  2:7932504/1‑150 (MQ=255)
                                                                                aCTCGAAGGCATGGGCGTCGGGTGGCGGGTCGGCGGCGGGATAGAGCTGCACCACGGCATCGGGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCACGGCTGGGTCTTCCAGCAGGCGCAGGCTCCTAGCCTGCGCCGCGTCCg                                                                    >  2:8504020/1‑150 (MQ=255)
                                                                                 cTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGCATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCTGGGTCTTCCAGCAGGCGCAGGCTCCAAGCCTGCGCCGCGTCCgg                                                                   >  2:8896272/1‑150 (MQ=255)
                                                                                 cTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGCATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCTGGGTCTTCCAGCAGGCGCAGGCTCCAAGCCTGCGCCGCGTCCgg                                                                   >  2:8800102/1‑150 (MQ=255)
                                                                                  tCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGAGAGAGCTGCACCACGGCATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCTAGGCTGGGTCTTCCAGCAGGCGCAGGCTCCAAGCCTGCGCCGCGTCCGGc                                                                  >  2:4374493/1‑150 (MQ=255)
                                                                                     aaGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGCATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCTGGGTCTTCCAGCAGGCGCAGGCTCCAAGCCTGCGCCGCGTCCGGCCAg                                                               >  2:7821821/1‑150 (MQ=255)
                                                                                          aTGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGCATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCTGGGTCTTCCAGCAGGCGCAGGCTCCAAGCCTGCGCCGCGTCCGGCCAgcggcg                                                          >  2:4895342/1‑150 (MQ=255)
                                                                                                      tGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGCATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCTGGGTCTTCCAGCAGGCGCAGGCTCCAAGCCTGCGCCGCGTCCGGCCAGCGGCGACGGCGCAGCCa                                              >  1:2918749/1‑150 (MQ=255)
                                                                                                       ggCGGGTCGGCGTCGGGATAGAGCTGCACCACGGCATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCTGGGTCTTCCAGCAGGCGCAGGCTCCAAGCCTGCGCCGCGTCCGGCCAGCGGCGACGGCGCAGCCAg                                             >  2:10990274/1‑150 (MQ=255)
                                                                                                         cGGGTCGGCGTCGGGATAGAGCTGCACCACGGCATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCTGGGTCTTCCAGCAGGCGCAGGCTCCAAGCCTGCGCCGCGTCCGGCCAGCGGCGACGGCGCAGCCAGTc                                           >  2:8304959/1‑150 (MQ=255)
                                                                                                          gggTCGGCGTCGGGATAGAGCTGCACCACGGCATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCTGGGTCTTCCAGCAGGCGCAGGCTCCAAGCCTGCGCCGCGTCCGGCCAGCGGCGACGGCGCAGCCAGTcg                                          >  1:302210/1‑150 (MQ=255)
                                                                                                           ggTCGGCGTCGGGATAGAGCTGCACCACGGCATCGTGGTCAGGGAACCAGGCCGGCTGCGCGTCGCGCGCATCCAAGGCGGGGTCTTCCAGCAGGCGCAGGCTCCAATCCTGCGCCGCGTCCGGCCCGCGGCGACGGCGCCGCCAGTcgc                                         >  2:9100324/1‑150 (MQ=255)
                                                                                                            gTCGGCGTCGGGATAGAGCTGCACCACGGCATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCTGGGTCTTCCAGCAGGCGCAGGCTCCAAGCCTGCGCCGCGTCCGGCCAGCGGCGACGGCGCAGCCAGTcgcg                                        >  2:9186128/1‑150 (MQ=255)
                                                                                                                gCGTCGGGATAGAGCTGCACCACGGCATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCTGGGTCTTCCAGCAGGCGCAGGCTCCAAGCCTGCGCCGCGTCCGGCCAGCGGCGACGGCGCAGCCAGTCGcggcgg                                    >  1:10534330/1‑150 (MQ=255)
                                                                                                                 cGTCGGCATACAGCTCCCCCCCGCCATCGCGGCCTGCCAACCACGCCGCATGCGCCTCGCGCCCAACCAAGGCTGGGACTTCCCGACGGCGCAGGCGCCACGCCGGCGCCGCGTCCGGCCAGCGGCGACGGCGCAGCCAGTCGCGGCGGt                                   <  2:5564971/150‑1 (MQ=255)
                                                                                                                    cGGGATAGAGCTGCACCACGGCAACGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCTGGGTCTTCCAGCAGGCGCAGGCTCCAAGCCTGCGCCGCGTCCGGCCAGCGGCGACGGCGCAGCCAGTCGCGGCGGTAAt                                >  2:3785900/1‑150 (MQ=255)
                                                                                                                     gggATAGAGCTGCACCACGGCATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCTGGGTCTTCCAGCAGGCGCAGGCTCCAAGCCTGCGCCGCGTCCGGCCAGCGGCGACGGCGCAGCCAGTCGCGGCGGTAATc                               >  1:8862369/1‑150 (MQ=255)
                                                                                                                             gCTGCACCACGGCATCGTGGTCTGGTAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCTGGGTCTTCCAGCCGGCGCAGGCTCCAAGCCTGCGCCGCGTCCGGCCAGCGGCGACGGCGCAGCCAGTCGCGGCGGTAATCGGGATTcc                       <  2:6230334/150‑1 (MQ=255)
                                                                                                                                  accacGGCATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCAAAGGCTGGGTCTTCCAGAAGGCGCAGGCTCCAAGCATGCGCCGCGTCCGGCCAGCGGCGACGGCGCAGCCAGTCGCGGCTGTAATCGGGATTCCTgcgc                  >  2:5124993/1‑150 (MQ=255)
                                                                                                                                   ccacGGCATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCTGGGTCTTCCAGCAGGCGCAGGCTCCCAGCCTGCGCCGCGTCCGGCCAGCGGCGACGGCGCAGCCAGTCGCGGCGGTAATCGGGATTCCTGCGCa                 >  1:7321618/1‑150 (MQ=255)
                                                                                                                                      cGGCATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCTGGGTCTTCCAGCAGGCGCAGGCTCCAAGCCTGCGCCGCGTCCGGCCAGCGGCGACGGCGCAGCCAGTCGCGGCGGTAATCGGGATTCCTGCGCAGGt              >  2:9357153/1‑150 (MQ=255)
                                                                                                                                        gCATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCTGGGTCTTCCAGCAGGCGCAGGCTCCAAGCCTGCGCCGCGTCCGGCCAGCGGCGACGGCGCAGCCAGTCGCGGCGGTAATCGGGATTCCTGCGCAGGTAt            >  1:8500185/1‑150 (MQ=255)
                                                                                                                                         cATCGTGGTCAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCTGGGTCTTCCAGCAGGCGCAGGCTCCAAGCCTGCGCCGCGTCCGGCCAGCGGCGACGGCGCAGCCAGTCGCGGCGGTAATCGGGATTCCTGCGCAGGTAtt           >  1:930147/1‑150 (MQ=255)
                                                                                                                                           tCGTGGTCAGGGACCCTGGCCGGATGCGCGTCGGGCGCATCCACGGCTGGGTCTTCCAGCTGGCGCAGGCTCCAAGCCTGCGCCGCGTCCGGCCAGCGGCGACGGCGCAGCCAGTCGCGGCGGTAATCGGGATTCCTGCGCAGGTATTcc         <  2:5238811/150‑1 (MQ=255)
                                                                                                                                                gTCAGGGAACCAGGCCGGAGGCGCGTCGCGCGCAGCCAAGGCTGGGTCTTCCCGCCGGCGCCGGCTCCAAGCCTGCGCCGCGTCCGGCCAGCGGCGACGGCGCAGCCAGTCGCGGCGGTAATCGGGATTCCTGCGCAGGTAGTCCCAAGc    <  2:10859088/150‑1 (MQ=255)
                                                                                                                                                  cAGGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCTGGGTCTTCCAGCAGGCGCAGGCTCCAAGCCTGCGCCGCGTCCGGCCAGCGGCGACGGCGCAGCCAGTCGCGGCGGTAATCGGGATTCCTGCGCAGGTATTCCCAAGccc  >  1:1536829/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GCAGCAGCCGGGCCAATGCGACACCAGCACCAGGCGCTTGCCGTCGTGGATCAGTTGCTTGCGGCCGGGAACGCGCCAGAACTCGAAGGCATGGGCTTCGGGTGGCGGGTCGGCGTCGGGATAGAGCTGCACCACGGCATCGTGGTCANGGAACCAGGCCGGATGCGCGTCGCGCGCATCCAAGGCTGGGTCTTCCAGCAGGCGCAGGCTCCAAGCCTGCGCCGCGTCCGGCCAGCGGCGACGGCGCAGCCAGTCGCGGCGGTAATCGGGATTCCTGCGCAGGTATTCCCAAGCCA  >  NC_008463/2707579‑2707874

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑