Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 2,762,030 A→G K127E (AAG→GAG)  PA14_RS12995 → lysophospholipid acyltransferase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084632,762,0300AG100.0% 1436.9 / NA 369K127E (AAG→GAG) PA14_RS12995lysophospholipid acyltransferase family protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (182/187);  total (182/187)

Only 100 of 369 total aligned reads displayed.

TACGTGATCAACAAGGTGTTCAACGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCAAGGGCACCCGCAACCTGAACAACAAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCAAGGGCCGCATGCTGCCGTTGCCGCTGCTCTGC  >  NC_008463/2761883‑2762170
                                                                                                                                                   |                                                                                                                                            
 aCGTGATCAACAAGGTGTTCAACGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAgg                                                                                                                                           >  2:8869020/1‑150 (MQ=255)
 aCGTGATCAACAAGGTGTTCAACGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAgg                                                                                                                                           >  2:206581/1‑150 (MQ=255)
  cGTGATCAACAAGGTGTTCAACGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGt                                                                                                                                          <  1:3674536/150‑1 (MQ=255)
    tGATCAACAAGGTGTTCAACGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCa                                                                                                                                        >  1:7578590/1‑150 (MQ=255)
    tGATCAACAAGGTGTTCAACGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCa                                                                                                                                        <  1:6023500/150‑1 (MQ=255)
      aTCAACAAGGTGTTCAACGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAg                                                                                                                                      <  1:3640275/150‑1 (MQ=255)
         aacaaGGTGTTCAACGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTg                                                                                                                                   >  1:9296403/1‑150 (MQ=255)
         aacaaGGTGTTCAACGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTg                                                                                                                                   >  2:4331373/1‑150 (MQ=255)
          acaaGGTGTTCAACGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGt                                                                                                                                  >  2:3146200/1‑150 (MQ=255)
              ggTGTTCAACGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGcc                                                                                                                              >  1:5335683/1‑150 (MQ=255)
               gtgtTCAACGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCa                                                                                                                             <  1:5021978/150‑1 (MQ=255)
                tgtTCAACGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCAt                                                                                                                            >  1:6691413/1‑150 (MQ=255)
                 gtTCAACGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTGGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCAtt                                                                                                                           <  1:9798843/150‑1 (MQ=255)
                  tacAACGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTc                                                                                                                          <  1:2577791/148‑1 (MQ=255)
                    cAACGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCaa                                                                                                                        <  1:1328648/150‑1 (MQ=255)
                    cAACGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAAACTGGACGACGAGGTCAAGCTGTTGCCATTCaa                                                                                                                        <  2:2811154/150‑1 (MQ=255)
                        ggCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGc                                                                                                                    >  2:3431946/1‑150 (MQ=255)
                        ggCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGc                                                                                                                    <  2:243935/150‑1 (MQ=255)
                          cGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCgg                                                                                                                  >  1:7120908/1‑150 (MQ=255)
                          cGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCgg                                                                                                                  <  1:3077646/150‑1 (MQ=255)
                           gTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGt                                                                                                                 <  2:9800845/150‑1 (MQ=255)
                           gTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGt                                                                                                                 <  2:4751319/150‑1 (MQ=255)
                            tGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCTGCGATTCGCTGATCAGCTTCCCCGAGGGCACCCGCATCCTGGACGCAGAGGTCAAGCTGTTGCCATTCAAGAGCGGTa                                                                                                                <  2:10370350/150‑1 (MQ=255)
                             gCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTag                                                                                                               >  2:5096574/1‑149 (MQ=255)
                               tGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCt                                                                                                             <  2:3982905/150‑1 (MQ=255)
                               tGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCt                                                                                                             <  2:7575311/150‑1 (MQ=255)
                                ggTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTa                                                                                                            <  2:5301002/150‑1 (MQ=255)
                                ggTCGACCGCCAGCGCAGCGATGCCAATGCCAAGCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTa                                                                                                            <  2:1376082/150‑1 (MQ=255)
                                     aCCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTaccacc                                                                                                       >  2:6745606/1‑150 (MQ=255)
                                      ccGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCACCCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCt                                                                                                      >  2:8019665/1‑150 (MQ=255)
                                      ccGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCt                                                                                                      >  2:89807/1‑150 (MQ=255)
                                         ccAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTc                                                                                                   >  2:4489461/1‑150 (MQ=255)
                                          cagcgcagcgATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTcc                                                                                                  >  1:10261283/1‑150 (MQ=255)
                                          cagcgcagcgATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTcc                                                                                                  >  2:10797287/1‑150 (MQ=255)
                                            gcgcagcgATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCt                                                                                                <  1:4212220/150‑1 (MQ=255)
                                              gcagcgATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTgg                                                                                              >  2:1228054/1‑150 (MQ=255)
                                                agcgATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGcc                                                                                            <  1:3183844/150‑1 (MQ=255)
                                                    atgccaatgccaATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCAcc                                                                                        <  2:6674308/150‑1 (MQ=255)
                                                    atgccaatgccaATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCAcc                                                                                        <  2:11056468/150‑1 (MQ=255)
                                                        caatgccaatCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGa                                                                                    >  2:6855724/1‑150 (MQ=255)
                                                        caatgccaatCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGa                                                                                    <  1:4968250/150‑1 (MQ=255)
                                                        caatgccaatCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGa                                                                                    <  1:2516577/150‑1 (MQ=255)
                                                          atgccaatCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAgg                                                                                  >  1:4785621/1‑150 (MQ=255)
                                                          atgccaatCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAgg                                                                                  <  1:2646988/150‑1 (MQ=255)
                                                          atgccaatCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAgg                                                                                  <  1:9534816/150‑1 (MQ=255)
                                                          atgccaatCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACACGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTGTCTACCACCTGTCCCTGGCCCACCCGGAgg                                                                                  >  2:1677119/1‑150 (MQ=255)
                                                           tgccaatCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGt                                                                                 <  1:10510729/150‑1 (MQ=255)
                                                           tgccaatCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGt                                                                                 <  2:7033570/150‑1 (MQ=255)
                                                             ccaatCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCg                                                                               >  2:10665533/1‑150 (MQ=255)
                                                               aatCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAg                                                                             <  2:4649712/150‑1 (MQ=255)
                                                               aatCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTAGCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAg                                                                             >  2:4266563/1‑150 (MQ=255)
                                                                atCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGc                                                                            <  2:857266/150‑1 (MQ=255)
                                                                atCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGc                                                                            >  2:8154092/1‑150 (MQ=255)
                                                                     cTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTg                                                                       >  1:6171712/1‑150 (MQ=255)
                                                                      tCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGc                                                                      <  1:6512164/150‑1 (MQ=255)
                                                                       cGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGcc                                                                     >  1:5937258/1‑150 (MQ=255)
                                                                         aaCCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCgg                                                                   >  1:8891812/1‑150 (MQ=255)
                                                                           ccccTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTc                                                                 <  2:6913543/150‑1 (MQ=255)
                                                                             ccTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTctg                                                               >  1:1433317/1‑150 (MQ=255)
                                                                             ccTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTctg                                                               >  2:4171579/1‑150 (MQ=255)
                                                                             ccTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTctg                                                               <  2:8336832/150‑1 (MQ=255)
                                                                               tCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTctggc                                                             <  2:5958517/150‑1 (MQ=255)
                                                                                cgcccGCGCCCTGGACGGCGGCGATTCGCTGATCACCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTctggct                                                            <  1:800986/147‑1 (MQ=255)
                                                                                  aCCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTctggctgg                                                          <  2:7861776/150‑1 (MQ=255)
                                                                                     gcgcCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCa                                                       <  2:3949925/150‑1 (MQ=255)
                                                                                         ccTGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCt                                                   <  1:2796987/150‑1 (MQ=255)
                                                                                           tGGACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCa                                                 >  2:8902465/1‑150 (MQ=255)
                                                                                             gACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAAc                                               >  2:1417877/1‑150 (MQ=255)
                                                                                             gACGGCGGCGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAAc                                               <  2:5509599/150‑1 (MQ=255)
                                                                                                ggcggcGATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCgg                                            >  2:3012309/1‑150 (MQ=255)
                                                                                                 gcggcgATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCggg                                           >  2:6475515/1‑150 (MQ=255)
                                                                                                 gcggcgATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCggg                                           >  2:9483442/1‑150 (MQ=255)
                                                                                                  cggcgATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGt                                          >  1:8336592/1‑150 (MQ=255)
                                                                                                      gATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATg                                      >  1:225773/1‑150 (MQ=255)
                                                                                                      gATTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATg                                      >  1:3849725/1‑150 (MQ=255)
                                                                                                       aTTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGc                                     <  1:10117935/150‑1 (MQ=255)
                                                                                                       aTTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGc                                     <  1:5735494/150‑1 (MQ=255)
                                                                                                       aTTCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGc                                     >  2:1892392/1‑150 (MQ=255)
                                                                                                         tCGCTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGccc                                   >  2:10902489/1‑150 (MQ=255)
                                                                                                            cTGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCAAg                                >  2:2224471/1‑150 (MQ=255)
                                                                                                             tGATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCAAgg                               >  1:335763/1‑150 (MQ=255)
                                                                                                              gATCATCTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCAAggg                              <  1:4689517/150‑1 (MQ=255)
                                                                                                               atcatcTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCGTGCCCCTGGGc                             >  2:8715479/1‑150 (MQ=255)
                                                                                                                   tcTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCACCCTCAACCGGGTCATGCCCAAGGGCCGCa                         >  1:7293784/1‑150 (MQ=255)
                                                                                                                   tcTTCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCAAGGGCCGCa                         >  1:1191936/1‑150 (MQ=255)
                                                                                                                     ttCCCCGAGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCAAGGGCCGCAtg                       >  1:1586032/1‑150 (MQ=255)
                                                                                                                          cgaGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCAAGGGCCGCATGCTGcc                  <  1:4488081/148‑1 (MQ=255)
                                                                                                                            aGGGCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCAAGGGCCGCATGCTGCCGt                <  2:5653713/150‑1 (MQ=255)
                                                                                                                              ggCACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCAAGGGCCGCATGCTGCCGTTg              >  1:5220361/1‑150 (MQ=255)
                                                                                                                                cACCCGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCAAGGGCCGCATGCTGCCGTTGcc            <  2:4449163/150‑1 (MQ=255)
                                                                                                                                  cccGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCAAGGGCCGCATGCTGCCGTTGCCgc          >  2:5818943/1‑150 (MQ=255)
                                                                                                                                  cccGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCAAGGGCCGCATGCTGCCGTTGCCgc          <  1:10014779/150‑1 (MQ=255)
                                                                                                                                  cacGCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCAGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCTGGTCATGCCCAAGGGCCGCATGCTGCCGTTGCCgc          <  2:615244/148‑1 (MQ=255)
                                                                                                                                     gCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCAAGGGCCGCATGCTGCCGTTGCCgctgc       >  2:7307395/1‑150 (MQ=255)
                                                                                                                                     gCAACCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCAAGGGCCGCATGCTGCCGTTGCCgctgc       >  2:3264221/1‑150 (MQ=255)
                                                                                                                                       aaCCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCAAGGGCCGCATGCTGCCGTTGCCGCTGctc     >  2:7657667/1‑150 (MQ=255)
                                                                                                                                       aaCCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCAAGGGCCGCATGCTGCCGTTGCCGCTGctc     <  1:5818763/150‑1 (MQ=255)
                                                                                                                                        aCCTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCAAGGGCCGCATGCTGCCGGTGCCGCTGctct    >  1:1952600/1‑150 (MQ=255)
                                                                                                                                         ccTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCAAGGGCCGCATGCTGCCGTTGCCGCTGCTCTg   <  1:1081332/150‑1 (MQ=255)
                                                                                                                                          cTGGACGACGAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCAAGGGCCGCATGCTGCCGTTGCCGCTGCTCTGc  >  2:6649619/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                            
TACGTGATCAACAAGGTGTTCAACGGCGTGCTGGTCGACCGCCAGCGCAGCGATGCCAATGCCAATCCGCTCGAACCCCTCGACCGCGCCCTGGACGGCGGCGATTCGCTGATCATCTTCCCCAAGGGCACCCGCAACCTGAACAACAAGGTCAAGCTGTTGCCATTCAAGAGCGGTATCTACCACCTGTCCCTGGCCCACCCGGAGGTCGAGCTGGTGCCGGTCTGGCTGGCCAACCTCAACCGGGTCATGCCCAAGGGCCGCATGCTGCCGTTGCCGCTGCTCTGC  >  NC_008463/2761883‑2762170

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑