BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_008463	3,673,534	3 bp→CTC	intergenic (+83/+13)	fabI → / ← PA14_RS16690	enoyl‑ACP reductase FabI/SurA N‑terminal domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_008463	3,673,534	0	T	C	99.7%	1439.2 / NA	373	intergenic (+83/+15)	fabI/PA14_RS16690	enoyl‑ACP reductase FabI/SurA N‑terminal domain‑containing protein
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (229/143); minor base G (1/0); total (230/143)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*	NC_008463	3,673,535	0	C	T	96.6%	1525.1 / 23.2	386	intergenic (+84/+14)	fabI/PA14_RS16690	enoyl‑ACP reductase FabI/SurA N‑terminal domain‑containing protein
Reads supporting (aligned to +/- strand): ref base C (5/8); new base T (229/143); total (235/151)
Fisher's exact test for biased strand distribution p-value = 1.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.81e-01
*	NC_008463	3,673,536	0	T	C	96.6%	1436.9 / 32.7	386	intergenic (+85/+13)	fabI/PA14_RS16690	enoyl‑ACP reductase FabI/SurA N‑terminal domain‑containing protein
Reads supporting (aligned to +/- strand): ref base T (5/8); new base C (230/143); total (235/151)
Fisher's exact test for biased strand distribution p-value = 1.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.41e-01

Only 100 of 386 total aligned reads displayed.

TCCTCTACGTCGATGGCGGCTTCAACACTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGNGTAAACGGCCTTTTTCT‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCTCGTCGTCGGAGATAGCCTCCTCCGGCT  >  NC_008463/3673393‑3673679

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tCCTCTACGTCGAGGGCGGCTTCAACCCTCCCGCCATGGGCCCGCTGGACGACGACTTATCACCCCGCTCATGAAAAATGGCCGCTGCGTGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCAtc                                                                                                                                           <  1:8908938/150‑1 (MQ=255)

       cGTCGATGGCGGCTTCAACACTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGtct                                                                                                                                    >  1:6665868/1‑150 (MQ=255)

          cGATGGCGGCTTCAACACTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAg                                                                                                                                 <  1:7795150/150‑1 (MQ=255)

           gATGGCGGCTTCAACACTACCACCATGGGCCCGCTGGACGACGACAGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGAGGCCAATTTCATGGGCGGAATTCAGAAAAGGACGCGTAAGCGGACTTGTCTC‑TGCATCTCGGTCTCAGt                                                                                                                                >  2:7191925/1‑150 (MQ=255)

            aTGGCGGCTTCAACACTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTa                                                                                                                               >  2:9104909/1‑150 (MQ=255)

            aTGGCGGCTTCAACACTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTa                                                                                                                               >  1:598891/1‑150 (MQ=255)

             tGGCGGCTTCAACACTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTAt                                                                                                                              <  1:6792940/150‑1 (MQ=255)

                 ggcTTCAACACTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTtct                                                                                                                          >  1:8662559/1‑150 (MQ=255)

                     tCAACACTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGAc                                                                                                                      <  2:5826860/150‑1 (MQ=255)

                      cAACACTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACt                                                                                                                     >  1:10858233/1‑150 (MQ=255)

                       aaCACTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAATGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACtt                                                                                                                    >  2:7990158/1‑150 (MQ=255)

                       aaCACTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑GGCATCTCGGTCTCAGTATTTCTCGACtt                                                                                                                    >  2:8470737/1‑150 (MQ=255)

                        acacTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTc                                                                                                                   >  1:1286547/1‑150 (MQ=255)

                        acacTACCGCCATGGGCCCGCTGGACGACGACGGATCACCCCGCTCAGGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATACTGAAAAGGCCGCGTCAGCGTCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTc                                                                                                                   <  2:3371155/150‑1 (MQ=255)

                           cTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAATAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGc                                                                                                                >  1:10651356/1‑150 (MQ=255)

                            ccccGCCCGGGGCCCGCTGCAAGACGACTGATCACCCCGCTCATGAAACAGGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGcc                                                                                                               <  2:6577572/148‑1 (MQ=255)

                             aCCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCt                                                                                                              <  2:6047332/150‑1 (MQ=255)

                                gCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGAAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGt                                                                                                           >  2:8652701/1‑150 (MQ=255)

                                 ccATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTc                                                                                                          >  1:969887/1‑150 (MQ=255)

                                   aTGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCt                                                                                                        >  1:9983289/1‑150 (MQ=255)

                                    tGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTg                                                                                                       >  1:1097748/1‑150 (MQ=255)

                                     gggCCCGCTGGACGACGACTTACCACCCCCATCATGAAAAATGGCCGCTACGAGCGGCATTTTTCATGGGCGCAAAAACGAAAAGGCCGCGTAAGCGCCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGc                                                                                                      <  1:5508714/150‑1 (MQ=255)

                                      ggCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCGTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCGGGTCCTGCa                                                                                                     >  1:2683612/1‑150 (MQ=255)

                                        cccGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGTAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAAc                                                                                                   <  1:446131/150‑1 (MQ=255)

                                           gCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGa                                                                                                >  1:2216673/1‑150 (MQ=255)

                                           gCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGa                                                                                                <  2:10680488/150‑1 (MQ=255)

                                           gCTGGACGACGACTGATCACCCCGCACATTAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGa                                                                                                <  1:3194228/150‑1 (MQ=255)

                                             tGGACGACTACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGCAATAACGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGAcg                                                                                              <  1:317214/150‑1 (MQ=255)

                                              ggACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGAcgg                                                                                             >  1:3745619/1‑150 (MQ=255)

                                               gacgacgacTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGAcggc                                                                                            >  1:9335086/1‑150 (MQ=255)

                                               gacgacgacTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGAcggc                                                                                            >  2:4193039/1‑150 (MQ=255)

                                               gacgacgacTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGAcggc                                                                                            >  2:2864878/1‑150 (MQ=255)

                                                  gacgacTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGa                                                                                         >  2:2686706/1‑150 (MQ=255)

                                                  gacgacTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGa                                                                                         <  2:6139706/150‑1 (MQ=255)

                                                   acgacTTATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCTTGGGGGTAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGaa                                                                                        <  1:8402746/150‑1 (MQ=255)

                                                     gacTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCCTCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAgg                                                                                      <  2:3481482/150‑1 (MQ=255)

                                                     gacTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATGTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAgg                                                                                      >  2:5000294/1‑150 (MQ=255)

                                                     gacTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCATTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAgg                                                                                      <  1:5646696/150‑1 (MQ=255)

                                                      acTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAggc                                                                                     <  1:8701624/150‑1 (MQ=255)

                                                       cTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAggcg                                                                                    >  2:232327/1‑150 (MQ=255)

                                                        tGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAACGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAggcgg                                                                                   <  2:1032379/150‑1 (MQ=255)

                                                               cccGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTc                                                                            >  2:6977710/1‑150 (MQ=255)

                                                                ccGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCt                                                                           >  2:8307710/1‑150 (MQ=255)

                                                                 cGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGACGGCGGCGAAGTCTg                                                                          >  2:10790838/1‑150 (MQ=255)

                                                                 cGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTg                                                                          <  2:742043/150‑1 (MQ=255)

                                                                        gAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTcc                                                                   >  2:8743943/1‑150 (MQ=255)

                                                                          aaaaTGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGc                                                                 >  1:495646/1‑150 (MQ=255)

                                                                           aaaTGGCCGCTTCGACCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCt                                                                >  2:9399796/1‑150 (MQ=255)

                                                                             aTGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTgc                                                              >  2:4627354/1‑150 (MQ=255)

                                                                              tGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTgcg                                                             >  1:2823893/1‑150 (MQ=255)

                                                                               ggCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTgcgc                                                            >  2:9605542/1‑150 (MQ=255)

                                                                                gCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGcgcg                                                           >  2:6436919/1‑150 (MQ=255)

                                                                                     ttCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGcc                                                      >  1:6329590/1‑150 (MQ=255)

                                                                                         aGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGa                                                  >  1:1482106/1‑150 (MQ=255)

                                                                                         aGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGa                                                  >  1:4737188/1‑150 (MQ=255)

                                                                                          gCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGaa                                                 <  1:10448492/150‑1 (MQ=255)

                                                                                            ggCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAgc                                               >  1:9132280/1‑150 (MQ=255)

                                                                                            ggCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAgc                                               >  1:2530073/1‑150 (MQ=255)

                                                                                            ggCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAgc                                               >  1:3827205/1‑150 (MQ=255)

                                                                                             gCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAgcg                                              >  2:3650301/1‑150 (MQ=255)

                                                                                             gCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAgcg                                              >  2:6687955/1‑150 (MQ=255)

                                                                                                    tCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTAc                                       <  2:2467650/150‑1 (MQ=255)

                                                                                                    tCATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTAc                                       <  2:5613223/150‑1 (MQ=255)

                                                                                                     cATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACa                                      >  1:5615642/1‑150 (MQ=255)

                                                                                                     cATGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACa                                      >  1:8030234/1‑150 (MQ=255)

                                                                                                      aTGGGCGGAATAAAGAAACGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACAt                                     <  2:521052/150‑1 (MQ=255)

                                                                                                      aTGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACAt                                     >  2:790728/1‑150 (MQ=255)

                                                                                                       tGGGCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATg                                    >  1:5275482/1‑150 (MQ=255)

                                                                                                         ggCGGAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGc                                  >  2:9792950/1‑150 (MQ=255)

                                                                                                            ggAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCtt                               >  1:5955724/1‑150 (MQ=255)

                                                                                                            ggAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCtt                               >  2:8155341/1‑150 (MQ=255)

                                                                                                            ggAATAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCtt                               >  2:5992174/1‑150 (MQ=255)

                                                                                                                tAAAGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCtcg                           <  1:10037165/150‑1 (MQ=255)

                                                                                                                 aaaGAAACGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCAGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGACGCGGCGGTACATGGCCTTCtcgt                          <  1:1526586/150‑1 (MQ=255)

                                                                                                                 aaaGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCtcgt                          >  2:3927771/1‑150 (MQ=255)

                                                                                                                  aaGCAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCtcgtc                         <  1:2270276/150‑1 (MQ=255)

                                                                                                                  aaGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCtcgtc                         >  2:1243333/1‑150 (MQ=255)

                                                                                                                  aaGAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCtcgtc                         >  1:7177482/1‑150 (MQ=255)

                                                                                                                    gAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCtcgtcgt                       >  1:1710216/1‑150 (MQ=255)

                                                                                                                    gAAAAGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCtcgtcgt                       >  1:6521387/1‑150 (MQ=255)

                                                                                                                    caaaaGGCCGCTTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCtcgtcgt                       <  1:7434162/149‑1 (MQ=255)

                                                                                                                     aaaaGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCtcgtcgtc                      >  1:3624693/1‑150 (MQ=255)

                                                                                                                     aaaaGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCtcgtcgtc                      <  2:2197905/150‑1 (MQ=255)

                                                                                                                       aaGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCTCGTCGTCgg                    >  1:4207180/1‑150 (MQ=255)

                                                                                                                       aaGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCTCGTCGTCgg                    >  2:7731380/1‑150 (MQ=255)

                                                                                                                       aaGGCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCTCGTCGTCgg                    >  2:290693/1‑150 (MQ=255)

                                                                                                                          gCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCTCGTCGTCGgaga                 >  1:10466858/1‑150 (MQ=255)

                                                                                                                          gCCGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCTCGTCGTCGgaga                 >  2:4417960/1‑150 (MQ=255)

                                                                                                                           ccGCTTAAGCGCCCTTTTCTC‑TGCATCTCGGTATCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCCGGAAGCGGCGGTACATGGCCTTCTCGTCGTCGGAGAt                <  1:10167727/150‑1 (MQ=255)

                                                                                                                           ccGCGTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCTCGTCGTCGGAGAt                >  2:1655760/1‑150 (MQ=255)

                                                                                                                            cgcgTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCTCGTCGTCGGCGATa               <  2:6328106/150‑1 (MQ=255)

                                                                                                                            cgcgTAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCTCGTCGTCGGAGATa               >  1:4220462/1‑150 (MQ=255)

                                                                                                                                tAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCCGGAAGCGGCGGTACATGGCCTTCTCGTCGTCGGAGATAGcct           <  1:6144094/150‑1 (MQ=255)

                                                                                                                                tAAGCGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCTCGTCGTCGGAGATAGcct           >  2:4923738/1‑150 (MQ=255)

                                                                                                                                  agcGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCTCGTCGTCGGAGATAGcctcc         >  2:1429633/3‑150 (MQ=255)

                                                                                                                                    cGGCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCTCGTCGTCGGAGATAGCctcctc       >  1:9756329/1‑150 (MQ=255)

                                                                                                                                     ggCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCTCGTCGTCGGAGATAGCCtcctcc      >  1:5182309/1‑150 (MQ=255)

                                                                                                                                     ggCCTTTTCTC‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCTCGTCGTCGGAGATAGCCtcctcc      >  1:2346411/1‑150 (MQ=255)

                                                                                                                                        ccttttCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCTCGTCGTCGGAGATAGCCTCCTCCgg    >  2:2122915/3‑150 (MQ=255)

                                                                                                                                         cttttCTCTGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCTCGTCGTCGGAGATAGCCTCCTCCGGc   >  1:3999949/2‑150 (MQ=255)

|||

TCCTCTACGTCGATGGCGGCTTCAACACTACCGCCATGGGCCCGCTGGACGACGACTGATCACCCCGCTCATGAAAAATGGCCGCTTCGAGCGGCCTTTTTCATGGGCGGAATAAAGAAAAGGCCGNGTAAACGGCCTTTTTCT‑TGCATCTCGGTCTCAGTATTTCTCGACTTCCGCCTTGTCCTGCAACTGACGGCGGAAGGCGGCGAAGTCTGCCTGTCCGCTGCGCGAAGCCAGGAAGCGGCGGTACATGGCCTTCTCGTCGTCGGAGATAGCCTCCTCCGGCT  >  NC_008463/3673393‑3673679

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`