BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_008463	5,103,224	2 bp→CA	coding (818‑819/1185 nt)	ftsZ ←	cell division protein FtsZ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_008463	5,103,224	0	A	C	100.0%	1848.6 / NA	476	P273M (CCT→CTG)	ftsZ	cell division protein FtsZ
Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (269/207); total (269/207)
*	NC_008463	5,103,225	0	G	A	99.2%	1939.9 / NA	476	P273M (CCT→CTG)	ftsZ	cell division protein FtsZ
Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (266/206); minor base C (3/1); total (269/207)
Fisher's exact test for biased strand distribution p-value = 6.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 476 total aligned reads displayed.

CTCCAGGCGCGCGCCCAGGCCGGTGGCGACTACGGTGACGTGCAGCTCATCGCGCATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCAGGGGACAGGTCCGGACCCGCGGTGATGTTCACCATGATGCCGCGCGCGCCCTGCAGGTTGACGTCTCCCAGCAGCGGGGTGCGGATTGCCGCCTCGGGGGCCTCGCGGGCACGGTTCGGACCGCTGGCGCAGCCGGTACCCATC  >  NC_008463/5103080‑5103368

||

  ccAGGCGCGCGCCCAGGCCGGTGGCGACTACGGTGACGTGCAGCTCATCGCGCATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACa                                                                                                                                           <  2:6788517/150‑1 (MQ=255)

    aGGCGCGCGCCCAGGCCGGTGGCGACTACGGTGACGTGCAGCTCATCGCGCATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAgg                                                                                                                                         <  2:10266125/150‑1 (MQ=255)

          gcgcCCAGGCCGGTGGCGACTACGGTGACGTGCAGCTCATCGCGCATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGa                                                                                                                                   <  1:2148168/150‑1 (MQ=255)

            gcCCAGGCCGGTGGCGACTACGGTGACGTGCAGCTCATCGCGCATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGAcc                                                                                                                                 <  1:486045/150‑1 (MQ=255)

            gcCCAGGCCGGTGGCGACTACGGTGACGTGCAGCTCATCGCGCATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGAcc                                                                                                                                 <  1:3664194/150‑1 (MQ=255)

             cccAGGCCGGTGGCGACTACGGTGACGTGCAGCTCATCGCGCATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGAccc                                                                                                                                >  2:7655268/1‑150 (MQ=255)

              ccAGGCCGGTGGCGACTACGGTGACGTGCAGCTCATCGCGCATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCcg                                                                                                                               <  2:262867/150‑1 (MQ=255)

               cAGGCCGGTGGCGACTACGGTGACGTGCAGCTCATCGCGCATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCcgc                                                                                                                              <  1:5896270/150‑1 (MQ=255)

               cAGGCCGGTGGCGACTACGGTGACGTGCAGCTCATCGCGCATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCcgc                                                                                                                              <  2:7532997/150‑1 (MQ=255)

               cAGGCCGGTGGCGACTACGGTGACGTGCAGCTCATCGCGCATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCcgc                                                                                                                              >  2:10844078/1‑150 (MQ=255)

               cAGGCCGGTGGCGACTACGGTGACGTGCAGCTCATCGCGCATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCcgc                                                                                                                              <  1:3943119/150‑1 (MQ=255)

                   ccGGTGGCGACTACGGTGACGTGCAGCTCATCGCGCATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTg                                                                                                                          <  2:971130/150‑1 (MQ=255)

                    cGGTGGCGACTACGGTGACGTGCAGCTCATCGCGCATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGa                                                                                                                         >  1:9111306/1‑150 (MQ=255)

                    cGGTGGCGACTACGGTGACGTGCAGCTCATCGCGCATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGa                                                                                                                         <  1:9512479/150‑1 (MQ=255)

                    cGGTGGCGACTACGGTGACGTGCAGCTCATCGCGCATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGa                                                                                                                         >  1:4574826/1‑150 (MQ=255)

                       tGGCGACTACGGTGACGTGCAGCTCATCGCGCATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGt                                                                                                                      >  1:6388193/1‑150 (MQ=255)

                       tGGCGACTACGGTGACGTGCAGCTCATCGCGCATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGt                                                                                                                      >  1:2807600/1‑150 (MQ=255)

                       tGGCGACTACGGTGACGTGCAGCTCATCGCGCATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGt                                                                                                                      >  2:6068985/1‑150 (MQ=255)

                        ggCGACTACGGTGACGTGCAGCTCATCGCGCATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGtt                                                                                                                     >  1:1904837/1‑150 (MQ=255)

                        ggCGACTACGGTGACGTGCAGCTCATCGCGCATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGtt                                                                                                                     >  1:8103986/1‑150 (MQ=255)

                         gCGACTACGGTGACGTGCAGCTCATCGCGCATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTc                                                                                                                    >  2:7113001/1‑150 (MQ=255)

                           gACTACGGTGACGTGCAGCTCATCGCGCATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCAc                                                                                                                  >  2:8188822/1‑150 (MQ=255)

                             cTACGGTGACGTGCAGCTCATCGCGCATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCa                                                                                                                <  2:4828009/150‑1 (MQ=255)

                             cTACGGTGACGTGCAGCTCATCGCGCATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCGCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCa                                                                                                                >  2:3331331/1‑150 (MQ=255)

                                cGGTGACGTGCAGCTCATCGCGCATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCagga                                                                                                             <  2:5653368/150‑1 (MQ=255)

                                  gTGACGTGCAGCTCATCGCGCATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCaggatg                                                                                                           >  2:8732876/1‑150 (MQ=255)

                                      cGTGCAGCTCATCGCGCATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCcgc                                                                                                       <  2:1863234/150‑1 (MQ=255)

                                      cGTGCAGCTCATCGCGCATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCcgc                                                                                                       >  1:10168122/1‑150 (MQ=255)

                                      cGTGCAGCTCATCGCGCATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCcgc                                                                                                       >  1:10382903/1‑150 (MQ=255)

                                        tGCAGCTCATCGCGCATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCcgcgc                                                                                                     <  1:7441746/150‑1 (MQ=255)

                                         gCAGCTCATCGCGCATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCcgcgcg                                                                                                    <  2:1648916/150‑1 (MQ=255)

                                         gCAGCTCATCGCGCATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCcgcgcg                                                                                                    >  2:10637807/1‑150 (MQ=255)

                                           aGCTCATCGCGCATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCcgcgcgcg                                                                                                  >  2:2956588/1‑150 (MQ=255)

                                           aGCTCATCGCGCATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCcgcgcgcg                                                                                                  >  2:1484189/1‑150 (MQ=255)

                                               cATCGCGCATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGGACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCt                                                                                              >  1:5557989/1‑150 (MQ=255)

                                                aTCGCGCATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTg                                                                                             >  1:7719967/1‑150 (MQ=255)

                                                 tCGCGCATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGc                                                                                            >  1:4298775/1‑150 (MQ=255)

                                                 tCGCGCATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGc                                                                                            >  2:4270451/1‑150 (MQ=255)

                                                   gcgcATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAg                                                                                          >  2:3174635/1‑150 (MQ=255)

                                                    cgcATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAgg                                                                                         >  2:3855844/1‑150 (MQ=255)

                                                     gcATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGt                                                                                        <  2:2413161/150‑1 (MQ=255)

                                                     gcATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGt                                                                                        <  1:1673526/150‑1 (MQ=255)

                                                       aTGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTg                                                                                      >  2:4848709/1‑150 (MQ=255)

                                                        tGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGa                                                                                     >  1:6080591/1‑150 (MQ=255)

                                                         gTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTGCGTTGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGAc                                                                                    >  2:8386405/1‑150 (MQ=255)

                                                           ccGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGt                                                                                  >  1:9883977/1‑150 (MQ=255)

                                                            cgcgTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGtc                                                                                 <  2:1315721/150‑1 (MQ=255)

                                                                 cGATCTCGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCca                                                                            <  2:10095408/150‑1 (MQ=255)

                                                                 cGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCca                                                                            <  2:8954096/150‑1 (MQ=255)

                                                                       cGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCg                                                                      >  1:8330620/1‑150 (MQ=255)

                                                                          tGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCgggt                                                                   <  2:1982547/150‑2 (MQ=255)

                                                                            cccACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTg                                                                 <  1:4635614/150‑1 (MQ=255)

                                                                            cccACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTg                                                                 <  2:5505792/150‑1 (MQ=255)

                                                                            cccACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTg                                                                 >  2:5790758/1‑150 (MQ=255)

                                                                             cccacTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGc                                                                <  2:6262627/146‑1 (MQ=255)

                                                                                 cTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGAt                                                            <  2:3694936/150‑1 (MQ=255)

                                                                                 cTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGAt                                                            <  1:1260218/150‑1 (MQ=255)

                                                                                     acaGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCGCCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTgcc                                                        >  2:4554960/1‑150 (MQ=255)

                                                                                         tGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCt                                                    >  1:4070036/1‑150 (MQ=255)

                                                                                               gCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCggtgg                                              >  2:6363564/1‑150 (MQ=255)

                                                                                                cTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCGGTGGc                                             <  1:9234678/150‑1 (MQ=255)

                                                                                                 tCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCGGTGGcc                                            <  1:6779657/150‑1 (MQ=255)

                                                                                                   ggAAGCTAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCGGTGGCCTc                                          <  2:4386728/150‑1 (MQ=255)

                                                                                                   ggAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCGGTGGCCTc                                          >  2:7975774/1‑150 (MQ=255)

                                                                                                   ggAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCGGTGGCCTc                                          >  1:2984709/1‑150 (MQ=255)

                                                                                                   ggAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCGGTGGCCTc                                          >  1:8719972/1‑150 (MQ=255)

                                                                                                     aaGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCGGTGGCCTcgc                                        >  1:10854324/1‑150 (MQ=255)

                                                                                                       gCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCGGTGGCCTCGCgg                                      >  1:9724591/1‑150 (MQ=255)

                                                                                                          aaCTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCGGTGGCCTCGCGGGCa                                   >  1:10516509/1‑150 (MQ=255)

                                                                                                          aaCTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCGGTGGCCTCGCGGGCa                                   <  1:7122466/150‑1 (MQ=255)

                                                                                                          aaCTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACCACTTCCAGCAGCGGGTTGCGGATTGCCGCCTCGGTGGCCTCGCGGGCa                                   >  1:6146421/1‑150 (MQ=255)

                                                                                                             tGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCGGTGGCCTCGCGGGCACgg                                >  1:1383336/1‑150 (MQ=255)

                                                                                                             tGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCGGTGGCCTCGCGGGCACgg                                >  1:2533647/1‑150 (MQ=255)

                                                                                                             tGTTCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCGGTGGCCTCGCGGGCACgg                                >  1:2215269/1‑150 (MQ=255)

                                                                                                               ttCGATGATGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCGGTGGCCTCGCGGGCACGGtt                              <  1:8519959/150‑1 (MQ=255)

                                                                                                                  gatgatGTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCGGTGGCCTCGCGGGCACGGTTCgg                           >  2:4021932/1‑150 (MQ=255)

                                                                                                                   atgatgTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCGGTGGCCTCGCGGGCACGGTTCGGa                          >  2:513056/1‑150 (MQ=255)

                                                                                                                   atgatgTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCGGTGGCCTCGCGGGCACGGTTCGGa                          >  2:6601329/1‑150 (MQ=255)

                                                                                                                    tgatgTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCGGTGGCCTCGCGGGCACGGTTCGGAc                         >  1:8205870/1‑150 (MQ=255)

                                                                                                                    tgatgTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCGGTGGCCTCGCGGGCACGGTTCGGAc                         >  1:10038406/1‑150 (MQ=255)

                                                                                                                    tgatgTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCGGTGGCCTCGCGGGCACGGTTCGGAc                         >  2:2210482/1‑150 (MQ=255)

                                                                                                                     gatgTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCGGTGGCCTCGCGGGCACGGTTCGGAcc                        >  1:7644516/1‑150 (MQ=255)

                                                                                                                      atgTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCGGTGGCCTCGCGGGCACGGTTCGGACCg                       >  1:6297964/1‑150 (MQ=255)

                                                                                                                        gTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCGGTGGCCTCGCGGGCACGGTTCGGACCGCt                     <  2:9293092/150‑1 (MQ=255)

                                                                                                                        gTTGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCGGTGGCCTCGCGGGCACGGTTCGGACCGCt                     <  1:8927529/150‑1 (MQ=255)

                                                                                                                         ttGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCGGTGGCCTCGCGGGCACGGTTCGGACCGCTg                    >  1:5215720/1‑150 (MQ=255)

                                                                                                                          tGCCGACATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCGGTGGCCTCGCGGGCACGGTTCGGACCGCTgg                   <  1:8174656/150‑1 (MQ=255)

                                                                                                                              gACAGCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCGGTGGCCTCGCGGGCACGGTTCGGACCGCTGGCGCa               >  2:4170160/1‑150 (MQ=255)

                                                                                                                               aCATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCGGTGGCCTCGCGGGCACGGTTCGGACCGCTGGCGCAg              <  2:11031160/150‑1 (MQ=255)

                                                                                                                                cATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCGGTGGCCTCGCGGGCACGGTTCGGACCGCTGGCGCAGc             <  2:10401720/150‑1 (MQ=255)

                                                                                                                                cATCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCGGTGGCCTCGCGGGCACGGTTCGGACCGCTGGCGCAGc             <  1:5686603/150‑1 (MQ=255)

                                                                                                                                  tCGGAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCGGTGGCCTCGCGGGCACGGTTCGGACCGCTGGCGCAGCCg           <  1:3168816/150‑1 (MQ=255)

                                                                                                                                     gAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCGGTGGCCTCGCGGGCACGGTTCGGACCGCTGGCGCAGCCGGTa        >  2:9132949/1‑150 (MQ=255)

                                                                                                                                     gAGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCGGTGGCCTCGCGGGCACGGTTCGGACCGCTGGCGCAGCCGGTa        >  2:9839642/1‑150 (MQ=255)

                                                                                                                                      aGTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCGGTGGCCTCGCGGGCACGGTTCGGACCGCTGGCGCAGCCGGTAc       <  2:7460960/150‑1 (MQ=255)

                                                                                                                                       gTACTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCGGTGGCCGCGCGGGCACGGTTCGGACCGCTGGCGCAGCCGGTAcc      >  2:8421423/1‑150 (MQ=255)

                                                                                                                                         aCTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCGGTGGCCTCGCGGGCACGGTTCGGACCGCTGGCGCAGCCGGTACCca    <  1:4954133/150‑1 (MQ=255)

                                                                                                                                          cTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCGGTGGCCTCGCGGGCACGGTTCGGACCGCTGGCGCAGCCGGTACCcat   >  2:868413/1‑150 (MQ=255)

                                                                                                                                          cTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCGGTGGCCTCGCGGGCACGGTTCGGACCGCTGGCGCAGCCGGTACCcat   >  1:8779532/1‑150 (MQ=255)

                                                                                                                                          cTCGCCCAGGGACAGGTCCGGACCCGCGGTGATGTTCACCAGGATGCCGCGCGCGCCCTGCAGGTTGACGTCTTCCAGCAGCGGGTTGCGGATTGCCGCCTCGGTGGCCTCGCGGGCACGGTTCGGACCGCTGGCGCAGCCGGTACCcat   >  2:5274943/1‑150 (MQ=255)

||

CTCCAGGCGCGCGCCCAGGCCGGTGGCGACTACGGTGACGTGCAGCTCATCGCGCATGTCCGCGTCGATCACGGTGCCCACCTTCACAGTGGCGTGCTCGGAAGCGAACTGTTCGATGATGTTGCCGACATCGGAGTACTCGCCAGGGGACAGGTCCGGACCCGCGGTGATGTTCACCATGATGCCGCGCGCGCCCTGCAGGTTGACGTCTCCCAGCAGCGGGGTGCGGATTGCCGCCTCGGGGGCCTCGCGGGCACGGTTCGGACCGCTGGCGCAGCCGGTACCCATC  >  NC_008463/5103080‑5103368

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`