Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 791,862 3 bp→GCT coding (1842‑1844/5430 nt) pchF → pyochelin non‑ribosomal peptide synthetase PchF

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_008463791,8620CG100.0% 1963.9 / NA 509A614A (GCC→GCG) pchFpyochelin non‑ribosomal peptide synthetase PchF
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (310/199);  total (310/199)
*NC_008463791,8630TC99.8% 1942.9 / NA 509W615M (TGG→CTG) pchFpyochelin non‑ribosomal peptide synthetase PchF
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (309/198);  minor base A (0/1);  total (310/199)
Fisher's exact test for biased strand distribution p-value = 3.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*NC_008463791,8640GT99.4% 2117.6 / NA 509W615M (TGG→CTG) pchFpyochelin non‑ribosomal peptide synthetase PchF
Reads supporting (aligned to +/- strand):  ref base G (1/2);  new base T (309/197);  total (310/199)
Fisher's exact test for biased strand distribution p-value = 5.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 509 total aligned reads displayed.

CGCGGTGATGCTGCCGAAAAGCGCCGCGCAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCCCCGAAGTGGTCGCCTGGGTCTGCCTGGAAAGCGATGTCCCGAACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGGACGACCTCGCCTACGTGATCTACACCTCCG  >  NC_008463/791721‑792004
                                                                                                                                             |||                                                                                                                                            
cgcgGTGATGCTGCCGAAAAGCGCCGCGCAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTg                                                                                                                                        <  1:8903772/150‑1 (MQ=255)
   ggTGATGCTGCCGAAAAGCGCCGCGCAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCt                                                                                                                                     >  2:5453113/1‑150 (MQ=255)
     tGATGCTGCCGAAAAGCGCCGCGCTATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGAGCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTgg                                                                                                                                   >  2:1218775/1‑150 (MQ=255)
     tGATGCTGCCGAAAAGCGCCGCGCAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACCTCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGACGTGGTCGCGCTGGTCTGCCTgg                                                                                                                                   >  2:64226/1‑150 (MQ=255)
     tGATGCTGCCGAAAAGCGCCGCGCAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTgg                                                                                                                                   >  1:1167634/1‑150 (MQ=255)
         gctgcCGAAAAGCGCCGCGCAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGACGTGGTCGCGCTGGTCTGCCTGGAAAg                                                                                                                               >  2:7037419/1‑150 (MQ=255)
         gctgcCGAAAAGCGCCGCGCAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAg                                                                                                                               >  2:7170977/1‑150 (MQ=255)
          ctgcCGAAAAGCGCCGCGCAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGc                                                                                                                              >  1:1847015/1‑150 (MQ=255)
           tgcCGAAAAGCGCCGCGCAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCg                                                                                                                             <  2:10928699/150‑1 (MQ=255)
              cGAAAAGCGCCGCGCAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATg                                                                                                                          <  2:1782855/150‑1 (MQ=255)
                aaaaGCGCCGCGCAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCCGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAACGCGATGTc                                                                                                                        >  2:2353805/1‑150 (MQ=255)
                aaaaGCGCCGCGCAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTc                                                                                                                        >  1:7620130/1‑150 (MQ=255)
                aaaaGCGCCGCGCAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTc                                                                                                                        >  1:560800/1‑150 (MQ=255)
                 aaaGCGCCGCGCAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTcc                                                                                                                       >  2:3683276/1‑150 (MQ=255)
                   aGCGCCGCGCAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCg                                                                                                                     >  2:3521182/1‑150 (MQ=255)
                    gcgcCGCGCAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCgg                                                                                                                    >  2:2218590/1‑149 (MQ=255)
                      gccgcGCAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGAc                                                                                                                  <  1:2490670/150‑1 (MQ=255)
                       ccgcGCAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACg                                                                                                                 >  1:519614/1‑150 (MQ=255)
                        cgcgCAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGc                                                                                                                >  1:2141218/1‑150 (MQ=255)
                        cgcgCAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGc                                                                                                                >  1:6248952/1‑150 (MQ=255)
                         gcgcAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGcc                                                                                                               >  1:2660538/1‑150 (MQ=255)
                         gcgcAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGcc                                                                                                               >  1:9662568/1‑150 (MQ=255)
                           gcAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCgg                                                                                                             >  1:8761293/1‑150 (MQ=255)
                            cAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGc                                                                                                            <  1:3853378/150‑1 (MQ=255)
                            cAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGc                                                                                                            >  1:6909295/1‑150 (MQ=255)
                             aaTTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCt                                                                                                           >  1:4280925/1‑150 (MQ=255)
                             aaTTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCt                                                                                                           >  2:5115719/1‑150 (MQ=255)
                              aTTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTg                                                                                                          >  1:7198182/1‑150 (MQ=255)
                               ttGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTgc                                                                                                         >  1:2864105/1‑150 (MQ=255)
                                tGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTTTGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGGTGGTCTGCGTGGCAAGCGATGTCCCGGAGGCCGGCTgcg                                                                                                        >  2:6053004/1‑150 (MQ=255)
                                 ggTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGGCCCGGACGCCGGCTgcgc                                                                                                       >  2:10584191/1‑150 (MQ=255)
                                  gTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGCCATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGcc                                                                                                      >  1:7266548/1‑150 (MQ=255)
                                   tCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCt                                                                                                     <  1:4022274/150‑1 (MQ=255)
                                    cgcgGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTg                                                                                                    <  1:1448072/150‑1 (MQ=255)
                                         tGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTgg                                                                                               >  1:5371503/1‑150 (MQ=255)
                                          gATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGTTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGc                                                                                              >  1:6372579/1‑150 (MQ=255)
                                            tCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCa                                                                                            >  1:10440548/1‑150 (MQ=255)
                                            tCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCAGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCa                                                                                            >  2:2054127/1‑150 (MQ=255)
                                             cGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCAt                                                                                           <  1:9529046/150‑1 (MQ=255)
                                                 aTCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGAc                                                                                       >  1:5991923/1‑150 (MQ=255)
                                                 aTCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGAc                                                                                       <  1:4157944/150‑1 (MQ=255)
                                                 aTCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGAc                                                                                       >  2:7459440/1‑150 (MQ=255)
                                                  tcctccAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGAcc                                                                                      >  2:570749/1‑150 (MQ=255)
                                                  tcctccAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGAcc                                                                                      >  2:6212298/1‑150 (MQ=255)
                                                     tccAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGc                                                                                   <  1:10605045/150‑1 (MQ=255)
                                                         ggCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGgc                                                                               <  2:481243/150‑1 (MQ=255)
                                                          gCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGgcc                                                                              >  1:8610694/1‑150 (MQ=255)
                                                          gCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGgcc                                                                              >  2:6920440/1‑150 (MQ=255)
                                                          gCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGgcc                                                                              >  1:9277036/1‑150 (MQ=255)
                                                           ccGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGgccg                                                                             <  1:5007432/150‑1 (MQ=255)
                                                            cGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGACGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGgccgc                                                                            >  2:9676668/1‑150 (MQ=255)
                                                                     cTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAgcgc                                                                   <  2:10335035/150‑1 (MQ=255)
                                                                       aTGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGACAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCt                                                                 >  2:2980295/1‑150 (MQ=255)
                                                                          tgCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGgc                                                              >  2:10687917/1‑150 (MQ=255)
                                                                          tgCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCACCGACAGCGCCTGgc                                                              <  1:1645142/150‑1 (MQ=255)
                                                                             cGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGgccgc                                                           >  2:7626699/1‑150 (MQ=255)
                                                                              ggTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGgccgcc                                                          <  1:227023/150‑1 (MQ=255)
                                                                               gTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCa                                                         >  2:9500266/1‑150 (MQ=255)
                                                                                tGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCAc                                                        <  2:7900778/150‑1 (MQ=255)
                                                                                tGGACATCCGCCAGCCGCCGCGGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCAc                                                        >  2:9227904/1‑150 (MQ=255)
                                                                                 ggACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCAcc                                                       <  1:4408679/150‑1 (MQ=255)
                                                                                    cATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCAccgcc                                                    >  1:10009767/1‑150 (MQ=255)
                                                                                    cATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCAccgcc                                                    >  2:8076807/1‑150 (MQ=255)
                                                                                      tCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCAccgcccg                                                  >  1:8458873/1‑150 (MQ=255)
                                                                                       ccGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCAccgcccgc                                                 <  2:6692808/150‑1 (MQ=255)
                                                                                          ccAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCgcggc                                              >  2:10131367/1‑150 (MQ=255)
                                                                                           cAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCgcggcg                                             >  2:3085478/1‑150 (MQ=255)
                                                                                           cAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCGGGCCGCCGACAGCGCCAGGCCGCCACCGCCCgcggcg                                             >  2:8571914/1‑150 (MQ=255)
                                                                                            cgccgccGCTGCGGCGCCCGGCGATCCTCGCCCGCGCCGTAGTGGTCGCGCGGGTCTGCCTGGATAGCTCTGTCCCGGCCGCCGGCGGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGCCAGCGCCTGGCCGCCACCGCCCGcggcgg                                            <  2:863321/149‑1 (MQ=255)
                                                                                             gccgccGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGcggcggt                                           >  2:1604742/1‑149 (MQ=255)
                                                                                             gccgccGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGa                                           <  1:1989698/150‑1 (MQ=255)
                                                                                               cgccgcTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCGGGCCGCGGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAgg                                         >  2:3379704/1‑150 (MQ=255)
                                                                                                 ccgcTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTg                                       >  2:8378356/1‑150 (MQ=255)
                                                                                                 ccgcTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTg                                       >  1:4537403/1‑150 (MQ=255)
                                                                                                  cgcTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTgg                                      >  1:9903778/1‑150 (MQ=255)
                                                                                                  cgcTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTgg                                      <  2:897342/150‑1 (MQ=255)
                                                                                                     tGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTggcgg                                   <  1:6681183/150‑1 (MQ=255)
                                                                                                      gcggcgCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTggcggc                                  >  1:3206749/1‑150 (MQ=255)
                                                                                                      gcggcgCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTggcggc                                  >  1:8143348/1‑150 (MQ=255)
                                                                                                         gcgcCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGga                               >  2:2784932/1‑150 (MQ=255)
                                                                                                             cAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGgacgac                           >  1:5877257/1‑150 (MQ=255)
                                                                                                              aGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGGACGAcc                          >  1:3601379/1‑150 (MQ=255)
                                                                                                              aGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGACGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGCCGGACGAcc                          >  1:10903493/1‑150 (MQ=255)
                                                                                                               ggCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGGACGACCt                         >  1:9230728/1‑150 (MQ=255)
                                                                                                               ggCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGGACGACCt                         >  2:4351899/1‑150 (MQ=255)
                                                                                                                gCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGGACGACCTc                        >  2:1128825/1‑150 (MQ=255)
                                                                                                                   aTCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGGACGACCTCGcc                     <  2:4927350/150‑1 (MQ=255)
                                                                                                                     ccTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGGACGACCTCGCCTa                   <  2:8779922/150‑1 (MQ=255)
                                                                                                                       tCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGAGGTCCCGGACGCCGGCTGCGCCTGCGTGGCCAGCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGGACGACCTCGCCTACg                 <  2:3912397/150‑1 (MQ=255)
                                                                                                                        cGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGGACGACCTCGCCTACGt                <  2:445829/150‑1 (MQ=255)
                                                                                                                          ccAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGGACGACCTCGCCTACGTGa              >  1:4723461/1‑150 (MQ=255)
                                                                                                                           cAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGGACGACCTCGCCTACGTGag             >  1:6559783/1‑149 (MQ=255)
                                                                                                                            aGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGGACGACCTCGCCTACGTGATc            <  2:1504519/150‑1 (MQ=255)
                                                                                                                            aGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGGACGACCTCGCCTACGTGATc            >  1:7911389/1‑150 (MQ=255)
                                                                                                                            aGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGGACGACCTCGCCTACGTGATc            <  2:9130068/150‑1 (MQ=255)
                                                                                                                            aGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGGACGACCTCGCCTACGTGATc            <  2:5915666/150‑1 (MQ=255)
                                                                                                                                   aaGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGGACGACCTCGCCTACGTGATCTACACCt     >  1:7435/1‑150 (MQ=255)
                                                                                                                                     gTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCCTCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGGACGACCTCGCCTCCGTGATCTACACCTcc   <  2:2716468/150‑1 (MQ=255)
                                                                                                                                     gTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGGACGACCTCGCCTACGTGATCTACACCTcc   >  1:774876/1‑150 (MQ=255)
                                                                                                                                     gTGGTCGCGCGGGACTGCCCGGAAAGCGCTGCCCCGGACGCCGGCTGCGCCCGCGTGGCCATCGACCGGCTGGCCGCCGACCGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGCCGGACGACCAGGCCTACGTGATCTACACCTcc   <  2:4288006/150‑1 (MQ=255)
                                                                                                                                             |||                                                                                                                                            
CGCGGTGATGCTGCCGAAAAGCGCCGCGCAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCCCCGAAGTGGTCGCCTGGGTCTGCCTGGAAAGCGATGTCCCGAACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGGACGACCTCGCCTACGTGATCTACACCTCCG  >  NC_008463/791721‑792004

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑