BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_008463	5,791,973	Δ1 bp	intergenic (+67/‑68)	nadE → / → choE	ammonia‑dependent NAD(+) synthetase/cholinesterase ChoE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_008463	5,791,973	0	A	.	100.0%	2895.1 / NA	581	intergenic (+67/‑68)	nadE/choE	ammonia‑dependent NAD(+) synthetase/cholinesterase ChoE
Reads supporting (aligned to +/- strand): ref base A (0/0); new base . (284/297); total (284/297)

Only 100 of 581 total aligned reads displayed.

TGCGCGAGGAAGCCGCGCGGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCAACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTCAGCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAAAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTCCTGCTGGCCGGCCAGGCCTGCGGACACACGTCCCCGCTGCTC  >  NC_008463/5791827‑5792118

   gcgAGGAAGCCGCGCGGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAg                                                                                                                                           <  2:5262654/151‑1 (MQ=255)

   gcgAGGAAGCCGCGCGGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAg                                                                                                                                           >  2:3815654/1‑151 (MQ=255)

    cgAGGAAGCCGCGCGGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAgg                                                                                                                                          >  1:7084612/1‑151 (MQ=255)

     gAGGAAGCCGCGCGGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGACCTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGt                                                                                                                                         <  2:3265700/151‑1 (MQ=255)

     gAGGAAGCCGCGCGGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGt                                                                                                                                         >  1:570049/1‑151 (MQ=255)

      aGGAAGCCGCGCGGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTc                                                                                                                                        >  1:6008068/1‑151 (MQ=255)

        gAAGCCGCGCGGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCtt                                                                                                                                      <  2:10236207/151‑1 (MQ=255)

        gAAGCCGCGCGGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCtt                                                                                                                                      >  1:2339596/1‑151 (MQ=255)

         aaGCCGCGCGGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTc                                                                                                                                     <  1:9965670/151‑1 (MQ=255)

          aGCCGCGCGGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCa                                                                                                                                    <  1:462126/151‑1 (MQ=255)

             cgcgcgGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGt                                                                                                                                 <  1:8835081/151‑1 (MQ=255)

              gcgcgGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTc                                                                                                                                <  1:8675066/151‑1 (MQ=255)

              gcgcgGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTc                                                                                                                                <  2:4740452/151‑1 (MQ=255)

               cgcgGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTcc                                                                                                                               <  1:1247202/151‑1 (MQ=255)

                gcgGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCcg                                                                                                                              >  2:6615495/1‑151 (MQ=255)

                gcgGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCcg                                                                                                                              >  1:3508367/1‑151 (MQ=255)

                 cgGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCcgc                                                                                                                             >  1:3241451/1‑151 (MQ=255)

                 cgGGAGATCGACGACACCTACCACCAGACCCAGCACAAGCGCGAACTGCCGACGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCcgc                                                                                                                             <  2:8265044/151‑1 (MQ=255)

                  gggTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCcgcg                                                                                                                            <  1:4831116/151‑1 (MQ=255)

                     tGATCGTCGACACCTACCACACGACCCAGCACACGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCATGCCCGTCGTCGACGGGCCTTTGTCGCGTGCGGGAATGTGCCTGTC‑GCAACCATGTCTTCAAGTCCGCgcgg                                                                                                                         <  2:8871835/151‑1 (MQ=255)

                     tGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGACTGTGCCTGTC‑GCACCCAGGTCTTCTAGTCCGCgcgg                                                                                                                         >  2:6483759/1‑151 (MQ=255)

                         cgtcgACCCCTACCCCCCGACCCAGCACACGCGCGAACTGCCGAAGGCGCCCAGCGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCATTGTCGCGGCCGGGAATGTGCCGGTC‑GCCACCACGACATCAAGTCCGCgcggcggc                                                                                                                     <  2:7972072/151‑1 (MQ=255)

                         cgtcgACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGACTGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCgcggcggc                                                                                                                     >  1:2792190/1‑151 (MQ=255)

                         cgtcgACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCgcggcggc                                                                                                                     <  1:9703769/151‑1 (MQ=255)

                          gtcgACACCTACCACAAGCCCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCgcggcggcg                                                                                                                    <  1:11284645/151‑1 (MQ=255)

                              acacCTACCACCAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCt                                                                                                                <  1:2207507/151‑1 (MQ=255)

                               cacCTACCACAAGACCCAGCACAAGCGCGACCTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTa                                                                                                               <  2:4101222/151‑1 (MQ=255)

                                acCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTag                                                                                                              >  2:902103/1‑150 (MQ=255)

                                 ccTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTaga                                                                                                             <  2:688070/151‑3 (MQ=255)

                                  cTACCACAAGTCCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTagaa                                                                                                            <  2:7553975/151‑1 (MQ=255)

                                  cTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTagaa                                                                                                            <  1:2879455/151‑1 (MQ=255)

                                    aCCACAAGACCCAGCACACGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAgg                                                                                                          <  1:10730715/151‑1 (MQ=255)

                                    aCCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGGGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAgg                                                                                                          <  1:4344727/151‑1 (MQ=255)

                                      cacaAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGaa                                                                                                        >  1:8176089/1‑151 (MQ=255)

                                        caAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGc                                                                                                      >  1:7110627/1‑151 (MQ=255)

                                         aaGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCt                                                                                                     <  2:784082/151‑1 (MQ=255)

                                         aaGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCt                                                                                                     <  2:3536822/151‑1 (MQ=255)

                                            aCCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGaa                                                                                                  >  1:1990989/1‑151 (MQ=255)

                                            aCCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGaa                                                                                                  <  2:11053198/151‑1 (MQ=255)

                                                aGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATccc                                                                                              >  1:9373159/1‑151 (MQ=255)

                                                   acaAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGc                                                                                           >  1:6368258/1‑151 (MQ=255)

                                                       gcgcGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAg                                                                                       <  1:8435454/151‑1 (MQ=255)

                                                        cgcgAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCACGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAgg                                                                                      <  2:5918305/151‑1 (MQ=255)

                                                        cgcgAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAgg                                                                                      >  1:1838839/1‑151 (MQ=255)

                                                          cgAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGgcc                                                                                    >  2:1604091/1‑151 (MQ=255)

                                                          cgAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGgcc                                                                                    >  1:502120/1‑151 (MQ=255)

                                                           gAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGgcca                                                                                   <  1:3815138/151‑1 (MQ=255)

                                                            aaCTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGgccag                                                                                  <  1:7908249/151‑1 (MQ=255)

                                                             aCTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGgccagc                                                                                 <  2:9202405/151‑1 (MQ=255)

                                                               tGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGgccagcca                                                                               <  1:11152643/151‑1 (MQ=255)

                                                                gCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCATTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCAt                                                                              <  2:7089925/151‑1 (MQ=255)

                                                                  cGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATgc                                                                            <  2:1270229/151‑1 (MQ=255)

                                                                    caGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATgcgc                                                                          <  1:3901064/150‑1 (MQ=255)

                                                                     aGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCa                                                                         <  1:10819045/151‑1 (MQ=255)

                                                                      ggCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCAc                                                                        <  1:5474356/151‑1 (MQ=255)

                                                                         gcCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTTTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTATAAGGAACCTGAATCCCTGCGAAGGCCAGCCATGCGCAccca                                                                     >  2:2865944/1‑150 (MQ=255)

                                                                         gcCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCg                                                                     >  1:6795359/1‑151 (MQ=255)

                                                                            cTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGtct                                                                  <  1:6663435/151‑1 (MQ=255)

                                                                            cTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGtct                                                                  <  1:6256443/151‑1 (MQ=255)

                                                                            cTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGtct                                                                  <  1:6043375/151‑1 (MQ=255)

                                                                               aGCCTCCCGGCCCGCGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGCAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCGccc                                                               <  1:11418879/151‑1 (MQ=255)

                                                                                gCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTcccc                                                              >  2:7621355/1‑151 (MQ=255)

                                                                                gCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTcccc                                                              >  2:953769/1‑151 (MQ=255)

                                                                                 ccTCCCGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCg                                                             <  1:7781469/151‑1 (MQ=255)

                                                                                     ccGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCCGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCt                                                         <  2:208843/151‑1 (MQ=255)

                                                                                     ccGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCt                                                         <  1:3752766/151‑1 (MQ=255)

                                                                                      cGGCCCGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTg                                                        >  1:1375721/1‑151 (MQ=255)

                                                                                          ccGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGctcc                                                    <  2:3006855/151‑1 (MQ=255)

                                                                                          ccGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGctcc                                                    <  2:1890713/151‑1 (MQ=255)

                                                                                           cGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGctcct                                                   >  2:5708989/1‑151 (MQ=255)

                                                                                           cGTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGctcct                                                   >  2:10510942/1‑151 (MQ=255)

                                                                                            gTGCGGCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGctcctc                                                  <  1:9194324/151‑1 (MQ=255)

                                                                                                ggCAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGgtg                                              >  1:4137719/1‑151 (MQ=255)

                                                                                                  cAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTc                                            <  1:8163642/151‑1 (MQ=255)

                                                                                                  cAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTc                                            >  2:10827781/1‑151 (MQ=255)

                                                                                                  cAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTc                                            >  2:2255100/1‑151 (MQ=255)

                                                                                                  cAGGCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTc                                            >  2:2791779/1‑151 (MQ=255)

                                                                                                     gCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTCctg                                         >  2:4627814/1‑151 (MQ=255)

                                                                                                     gCCCGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTCctg                                         <  1:5335312/151‑1 (MQ=255)

                                                                                                       ccGTCGTCGACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTCctgct                                       <  1:7343547/151‑1 (MQ=255)

                                                                                                        cgtcgtCGACGGGCCTATGTCTCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGACGGAAGCTGTATCCCCGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTCctgctg                                      <  2:1707139/151‑1 (MQ=255)

                                                                                                             tcgaCGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTCCTGCTggccgg                                 <  1:9939982/148‑1 (MQ=255)

                                                                                                               gatgggCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTCCTGCTggccggcc                               >  2:871572/4‑151 (MQ=255)

                                                                                                                aCGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTCCTGCTGGCCGGCCa                              >  1:10434668/1‑151 (MQ=255)

                                                                                                                aCGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTCCTGCTGGCCGGCCa                              >  2:251745/1‑151 (MQ=255)

                                                                                                                 cGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTCCTGCTGGCCGGCCAg                             >  1:7508269/1‑151 (MQ=255)

                                                                                                                  gggCCTTTGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTCCTGCTGGCCGGCCAgg                            >  2:11379375/1‑151 (MQ=255)

                                                                                                                       tttGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTCCTGCTGGCCGGCCAGGCCTGc                       >  2:4418517/1‑151 (MQ=255)

                                                                                                                       tttGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTCCTGCTGGCCGGCCAGGCCTGc                       >  2:6206460/1‑151 (MQ=255)

                                                                                                                       attGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTCCTGCTGGCCGGCCAGGCCTGc                       <  2:7277742/150‑1 (MQ=255)

                                                                                                                         tGTCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTCCTGCTGGCCGGCCAGGCCTGCgg                     >  2:8604929/1‑151 (MQ=255)

                                                                                                                           tCGCGGGCGGGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTCCTGCTGGCCGGCCAGGCCTGCGGac                   >  2:3179673/1‑151 (MQ=255)

                                                                                                                             gcgggcggGAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTCCTGCTGGCCGGCCAGGCCTGCGGacac                 <  1:51699/151‑1 (MQ=255)

                                                                                                                              cgggcgggAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTCCTGCTGGCCGGCCAGGCCTGCGGacaca                <  1:10164127/151‑1 (MQ=255)

                                                                                                                              cgggcgggAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTCCTGCTGGCCGGCCAGGCCTGCGGacaca                >  1:10050351/1‑151 (MQ=255)

                                                                                                                                   gggAATGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTCCTGCTGGCCGGCCAGGCCTGCGGACACACGTcc           >  2:10650776/1‑151 (MQ=255)

                                                                                                                                       aTGTGCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTCCTGCTGGCCGGCCAGGCCTGCGGACACACGTCCCCgc       >  1:7849469/1‑151 (MQ=255)

                                                                                                                                        tgtgCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTCCTGCTGGCCGGCCAGGCCTGCGGACACACGTCCCCgct      <  2:5426015/151‑1 (MQ=255)

                                                                                                                                         gtgCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTCCTGCTGGCCGGCCAGGCCTGCGGACACACGTCCCCgctg     >  1:1603237/1‑151 (MQ=255)

                                                                                                                                           gCCTGTC‑GCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAGAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTCCTGCTGGCCGGCCAGGCCTGCGGACACACGTCCCCgctgct   >  2:8427294/1‑151 (MQ=255)

TGCGCGAGGAAGCCGCGCGGGTGATCGTCGACACCTACCACAAGACCCAGCACAAGCGCGAACTGCCGAAGGCGCCCTGAGCCTCCCGGCCCGTGCGGCAGGCCCGTCGTCAACGGGCCTTTGTCGCGGGCGGGAATGTGCCTGTCAGCAACCAGGTCTTCAAGTCCGCGCGGCGGCGCGCTAAAAGGAAGCTGAATCCCTGCGAAGGCCAGCCATGCGCACCCGTCTCCCCGCCCTGCTCCTCGGTGTCCTGCTGGCCGGCCAGGCCTGCGGACACACGTCCCCGCTGCTC  >  NC_008463/5791827‑5792118

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`