Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 3,670,543 +A coding (543/1020 nt) PA14_RS16675 → ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084633,670,5421.A98.0% 1672.9 / 18.5 406coding (542/1020 nt)PA14_RS16675ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base . (7/1);  new base A (181/217);  total (188/218)
Fisher's exact test for biased strand distribution p-value = 2.72e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 435 total aligned reads displayed.

GACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCGTTTTCCTGG‑A‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGCCGAGTTCCTCCGCGGG  >  NC_008463/3670391‑3670687
                                                                                                                                                        |                                                                                                                                                  
gACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTgg                                                                                                                                                     <  2:757960/151‑1 (MQ=255)
 aCGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGG‑a                                                                                                                                                   <  1:7964451/151‑1 (MQ=255)
  cGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGG‑aa                                                                                                                                                  <  2:31443/151‑2 (MQ=255)
  cGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGG‑aa                                                                                                                                                  >  1:6176551/1‑150 (MQ=255)
  cGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGG‑aa                                                                                                                                                  >  2:5411412/1‑150 (MQ=255)
       tCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTg                                                                                                                                             <  1:7567211/151‑1 (MQ=255)
         gCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGt                                                                                                                                           >  2:8837376/1‑151 (MQ=255)
          cccGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTc                                                                                                                                          <  1:6027214/151‑1 (MQ=255)
             gggTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCggg                                                                                                                                       >  1:60731/1‑151 (MQ=255)
             gggTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCggg                                                                                                                                       <  1:833718/151‑1 (MQ=255)
               gTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTACACTCATCGGCGTCACCGTCGGCGCCCTGCAAGCCTTCTACGGCGGCTGGGTCGACCTGCTCGGCCTGCGTTACCAGGT‑A‑GTCTGGTCGGGGc                                                                                                                                     >  2:9122862/1‑151 (MQ=255)
                tGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCt                                                                                                                                    <  2:29975/151‑1 (MQ=255)
                  aTTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGc                                                                                                                                  >  2:3569557/1‑151 (MQ=255)
                   tttACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGcc                                                                                                                                 <  1:234/151‑1 (MQ=255)
                        ggCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGc                                                                                                                            >  1:5560919/1‑151 (MQ=255)
                              cgcgTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTAcc                                                                                                                      <  1:9469993/151‑1 (MQ=255)
                                cgTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACctg                                                                                                                    >  2:4565447/1‑151 (MQ=255)
                                cgTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACctg                                                                                                                    <  2:1058617/151‑1 (MQ=255)
                                cgTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACctg                                                                                                                    <  2:11338377/151‑1 (MQ=255)
                                  tgtCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACctgct                                                                                                                  >  2:5734682/1‑151 (MQ=255)
                                  tgtCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACctgct                                                                                                                  <  1:11020099/151‑1 (MQ=255)
                                     cGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGat                                                                                                               >  2:3393818/1‑151 (MQ=255)
                                         ccTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGatcatc                                                                                                           <  1:3939142/151‑1 (MQ=255)
                                          ctctTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATcc                                                                                                          >  1:10065902/1‑151 (MQ=255)
                                           tctTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCt                                                                                                         <  2:6894552/151‑1 (MQ=255)
                                            ctTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTg                                                                                                        <  2:6006677/151‑1 (MQ=255)
                                            ctTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGG‑ACGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTg                                                                                                        >  2:10162665/1‑151 (MQ=255)
                                               cGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCGACCTGCTGATCATCCTGGcc                                                                                                     <  2:1548269/151‑1 (MQ=255)
                                                gCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCa                                                                                                    >  2:11159366/1‑151 (MQ=255)
                                                 ccctgaccctgaCCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAg                                                                                                   >  1:9880968/1‑151 (MQ=255)
                                                  cctgaccctgacCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGc                                                                                                  <  1:3642332/151‑1 (MQ=255)
                                                   ctgaccctgaccGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCt                                                                                                 >  1:4082861/1‑151 (MQ=255)
                                                     gaccctgaccGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTc                                                                                               <  2:1393521/151‑1 (MQ=255)
                                                     gaccctgaccGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCAAGCTTc                                                                                               >  2:9011459/1‑151 (MQ=255)
                                                      accctgcccGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCg                                                                                              >  2:2524599/1‑151 (MQ=255)
                                                         ctgaccGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTcc                                                                                           <  1:494025/151‑1 (MQ=255)
                                                          tgaccGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCa                                                                                          <  1:5288325/151‑1 (MQ=255)
                                                           gaccGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAg                                                                                         <  1:3346448/151‑1 (MQ=255)
                                                           gaccGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGG‑ACGTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAg                                                                                         >  1:9161051/1‑151 (MQ=255)
                                                              cGTGCACAGTTATCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTCCGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCg                                                                                      <  2:677834/151‑1 (MQ=255)
                                                               gTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGa                                                                                     >  1:11233009/1‑151 (MQ=255)
                                                                tGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGaa                                                                                    <  1:6430090/151‑1 (MQ=255)
                                                                  cTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACt                                                                                  >  1:10814821/1‑151 (MQ=255)
                                                                     aGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCt                                                                               >  1:10543045/1‑151 (MQ=255)
                                                                          tccgctCGGCGTCACCGTCGGCGCCCAGCACGGCTCCGCCGGCGGCAGGGGCGACCAGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTCCCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCtggtgg                                                                          <  2:6528088/146‑1 (MQ=255)
                                                                           ctGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGgc                                                                         >  2:10692469/1‑151 (MQ=255)
                                                                            tGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGgct                                                                        <  1:1797768/151‑1 (MQ=255)
                                                                            tGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGgct                                                                        <  1:206493/151‑1 (MQ=255)
                                                                              aTCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGgctgc                                                                      >  1:6858615/1‑151 (MQ=255)
                                                                                cGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGctgctg                                                                    >  1:2554713/1‑151 (MQ=255)
                                                                                 ggCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTgg                                                                   <  2:3858168/151‑1 (MQ=255)
                                                                                  gCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTggg                                                                  <  2:396775/151‑1 (MQ=255)
                                                                                    gTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGca                                                                <  1:2214388/151‑1 (MQ=255)
                                                                                        ccGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGcatcat                                                            <  2:2093517/151‑1 (MQ=255)
                                                                                        ccGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGcatcat                                                            <  2:10947292/151‑1 (MQ=255)
                                                                                         cGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCAtg                                                           <  2:9611089/151‑1 (MQ=255)
                                                                                         cGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCAtg                                                           >  1:8657310/1‑151 (MQ=255)
                                                                                          gTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCAtgc                                                          >  2:7884257/1‑151 (MQ=255)
                                                                                           tCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCAtgct                                                         <  2:7362313/151‑1 (MQ=255)
                                                                                             ggCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCAtgctgc                                                       >  1:10342652/1‑151 (MQ=255)
                                                                                             ggCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCAtgctgc                                                       >  2:2803694/1‑151 (MQ=255)
                                                                                                gcCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGt                                                    >  2:7659308/1‑151 (MQ=255)
                                                                                                 cccTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGtt                                                   >  2:3079009/1‑151 (MQ=255)
                                                                                                  ccTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGttt                                                  <  1:3114210/151‑1 (MQ=255)
                                                                                                  ccTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGttt                                                  <  2:523739/151‑1 (MQ=255)
                                                                                                   cTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGtttt                                                 >  1:9289874/1‑151 (MQ=255)
                                                                                                    tGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTc                                                >  1:10679068/1‑151 (MQ=255)
                                                                                                    tGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTc                                                >  2:1235488/1‑151 (MQ=255)
                                                                                                      cAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCt                                              <  1:5125286/151‑1 (MQ=255)
                                                                                                       aaGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTg                                             <  1:8522628/151‑1 (MQ=255)
                                                                                                       aaGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTg                                             >  2:4283649/1‑151 (MQ=255)
                                                                                                        aGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTgg                                            <  1:9823802/151‑1 (MQ=255)
                                                                                                        aGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTgg                                            <  1:7300138/151‑1 (MQ=255)
                                                                                                              ctacGGCGGCTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGc                                      >  2:4658549/1‑151 (MQ=255)
                                                                                                                   gcggcTGGGTCGACCTGCTCGGCCAGCGTTTCCGGGA‑A‑GTCTGGTCGGGGCTGCCGGTGCTCTCCCTGCTGATCATCCTGGCCAGCGGCGTCCAGCCGAACTGCTGGTGGCTGCTGGGCAGCAGGCTGCTGTTTTCCTGGAGGTGCCtggt                                 >  2:8587827/1‑151 (MQ=255)
                                                                                                                   gcggcTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCtggt                                 >  1:8160833/1‑151 (MQ=255)
                                                                                                                       cTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTTGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGAc                             <  2:9784454/151‑1 (MQ=255)
                                                                                                                       cTGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGAc                             >  2:5175604/1‑151 (MQ=255)
                                                                                                                        tGGGTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACg                            >  2:10220460/1‑151 (MQ=255)
                                                                                                                         gggTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGt                           <  2:4874570/151‑1 (MQ=255)
                                                                                                                           gTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTgg                         <  2:221522/151‑1 (MQ=255)
                                                                                                                           gTCGACCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTgg                         >  1:784445/1‑151 (MQ=255)
                                                                                                                               aCCTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCcg                     <  2:630000/151‑1 (MQ=255)
                                                                                                                                ccTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCcgc                    >  2:6983282/1‑151 (MQ=255)
                                                                                                                                 cTGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCcgcg                   <  1:8741482/151‑1 (MQ=255)
                                                                                                                                  tGCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCgcgc                  >  1:45921/1‑151 (MQ=255)
                                                                                                                                   gCTCGGCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGcc                 <  1:2875606/151‑1 (MQ=255)
                                                                                                                                       ggCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGCCGAGt             <  1:8857436/151‑1 (MQ=255)
                                                                                                                                        gCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGCCGAGtt            <  1:674085/151‑1 (MQ=255)
                                                                                                                                        gCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGCCGAGtt            >  1:3563699/1‑151 (MQ=255)
                                                                                                                                        gCCAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGCCGAGtt            <  1:1596608/151‑1 (MQ=255)
                                                                                                                                         ccAGCG‑TTTCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGCCGAGTtc           >  2:3725925/1‑151 (MQ=255)
                                                                                                                                            agcgtttCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGCCGAGTtcct         <  1:9587262/147‑1 (MQ=255)
                                                                                                                                            agcgtttCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGCCGAGTtcct         <  1:4311209/147‑1 (MQ=255)
                                                                                                                                            agcgtttCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGCCGAGTtcct         >  1:11383831/5‑151 (MQ=255)
                                                                                                                                            agcgtttCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTGCGTCCAGCCGAACGTCTGGTGGCTGCTGGGCAGCATGCTGCTGGTGTCCGGGATGAGCCTGGTGGACGTGGTCCGCGCCGAGTgccg         >  2:8111534/5‑150 (MQ=255)
                                                                                                                                               gtttCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGCCGAGTTCCTCcg      >  1:654581/2‑151 (MQ=255)
                                                                                                                                                 ttCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGCCGAGTTCCTCcgcg    <  1:3803274/151‑1 (MQ=255)
                                                                                                                                                  tCCTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGCCGAGTTCCTCCGCgg   >  1:1275308/1‑151 (MQ=255)
                                                                                                                                                   ccTGGAA‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGCCGAGTTCCTCCGCggg  <  1:7010722/151‑1 (MQ=255)
                                                                                                                                                        |                                                                                                                                                  
GACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAGCGTTTTCCTGG‑A‑GTCTGGTCGGGGCTGCCGGTGCTCTACCTGCTGATCATCCTGGCCAGCTTCGTCCAGCCGAACTTCTGGTGGCTGCTGGGCATCATGCTGCTGTTTTCCTGGATGAGCCTGGTGGACGTGGTCCGCGCCGAGTTCCTCCGCGGG  >  NC_008463/3670391‑3670687

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑