Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084631,763,5840TG28.4% 226.9 / 19.2 183W30G (TGG→GGG) PA14_RS08240RHS repeat protein
Reads supporting (aligned to +/- strand):  ref base T (25/91);  new base G (46/0);  total (92/91)
Fisher's exact test for biased strand distribution p-value = 1.51e-22
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 186 total aligned reads displayed.

GTACGACGCCGAGGGACGCAAGACCGGCGAGACCGTCACCACCCGCTGACAAGGACGACCCCCATGCGCATCCCCCCATACTTCCCCCGTCTCGGCGCCCTCGCCCTGGCCTGCGCCCTGGCCCTGCCCGCGCAGGCGGCCGAGCGCGCCTGGAGCTACAGCTACAACGCCCTCGGCCTGATCGAGCGCGCCGACGGTCCGCGCACCGATGTGCAGGACGTCACCCTCTATGCCTACG  >  NC_008463/1763434‑1763671
                                                                                                                                                      |                                                                                       
gTACGACGCCGAGGGACGCAAGACCGGCGAGACCGTCACCACCCGCTGACAAGGACGACCCCCATGCGCATCCCCCCATACTTCCCCCGTCTCGGCGCCCTCGCCCTGGCCTGCGCCCTGGCCCTGCCCGCGCAGGCGGCCGAGCGCGCCt                                                                                         <  1:11405352/151‑1 (MQ=31)
   cgacgCCGAGGGACGCTAGACCGGCGAGACCGTCCCCCCCCGCTGACAAGGACGACCCCCATGCGCATCCCCCCATCCTTCCCCCGTCTCGGCGCCCTCGCCCTGGCCTGCGCCCTGGCCCTGCCCGCGCAGGCGGCCGAGCGCGCCTGGa                                                                                      <  2:2756070/151‑1 (MQ=9)
   cgacgCCGAGGGACGCAAGACCGGCGAGACCGTCACCACCCGCTGACAAGGACGACCCCCATGCGCGTCCCCCCATACTTCCCCCGTCTCGGCGCGCGCGCCCTGGCCTGCGGCCGGGCCCGGCCCGCGCCGGCGGCCGCGCGCGCCgggc                                                                                      >  1:5466814/1‑150 (MQ=11)
   cgacgCCGAGGGACGCAAGACCGGCGAGACCGTCACCACCCGCTGACAAGGACGACCCCCATGCGCATCCCCCCATACTTCCCCCGTCTCGGCGCCCTCGCCCTGGCCTGCGCCCTGGCCCTGCCCGCGCAGGCGGCCGAGCGCGCCTGGa                                                                                      <  2:10397194/151‑1 (MQ=31)
   cgacgCCGAGGGACGCAAGACCGGCGAGACCGTCACCACCCGCTGACAAGGACGACCCCCATGCGCATCCCCCCATACTTCCCCCGGCTCGGCGCCCTCGCCCTGGCCTGCGCCCTGGCCCTGCCCGCGCCGGCGGCCGGGCGCGCCgggg                                                                                      >  2:4308790/1‑150 (MQ=255)
       gCCGAGGGACGCAAGACCGGCGAGACCGTCACCACCCGCTGACAAGGACGACCCCCATGCGCATCCCCCCATGCTTCCCCCGTCTCGGCGCCCGCCCCCGGGCCGGCGCCCGGGCCCGGCCCGCGCCGGCGGGCGAGCGCGCCAGGggcga                                                                                  >  1:658677/1‑149 (MQ=11)
       gCCGAGGGACGCAAGACCGGCGAGACCGTCACCACCCGCTGACAAGGACGACCCCCATGCGCATCCCCCCATCCTTCCCCCGTCTCGGCGCCCTCGCCCTGGCCTGCGCCCTGGCCCTGCCCGCGCAGGCGGCCGAGCGCGCCTGGagcta                                                                                  <  2:4389186/151‑1 (MQ=14)
       gCCGAGGGACGCAAGACCGGCGAGACCGTCACCACCCGCTGACAAGGACGACCCCCATGCGCATCCCCCCATACTTCCCCCGTCTCGGCGCCCTCGCCCTGGCCTGCGCCCTGGCCCTGCCCGCGCAGGCGGCCGAGCGCGCCTGGagcta                                                                                  <  2:9741299/151‑1 (MQ=31)
       gCCGAGGGACGCAAGACCGGCGAGACCGTCACCACCCGCTGACAAGGACGACCCCCATGCGCATCCCCCCATACTTCCCCCGTCTCGGCGCCCTCGCCCTGGCCTGCGCCCTGGCCCTGCCCGCGCAGGCGGCCGAGCGCGCCTGGagcta                                                                                  <  2:7290398/151‑1 (MQ=31)
         cGAGGGACGCAAGACCGGCGAGACCGTCACCACCCGCTGACAAGGACGACCCCCATGCGCATCCCCCCATACTTCCCCCGTCTCGGCGCCCTCGCCCTGGCCTGCGCCCTGGCCCTGCCCGCGCAGGCGGCCGAGCGCGCCTGGagctaca                                                                                <  1:1428641/151‑1 (MQ=31)
          gAGGGACGCAAGACCGGCGAGACCGTCACCACCCGCTGACAAGGACGACCCCCATGCGCATCCCCCCATACTTCCCCCGTCTCGGCGCCGTCGCCCTGGCCTGCGCCCGGGGCCTGCCCGCGCGGGGGGCCGGGCGCGCCGGGggctgcag                                                                               >  1:6205218/1‑151 (MQ=11)
            gggACGCAAGACCGGCGAGACCGTCACCACCCGCTGACAAGGACGACCCCCATGCGCATCCCCCCATACTTCCCCCGTCTCGGCGCCCTCGCCCTGGCCTGCGCCCTGGCCCTGCCCGCGCAGGCGGCCGAGCGCGCCTGGagctacagct                                                                             <  1:4212357/151‑1 (MQ=31)
                cGCAAGACCGGCGAGACCGTCACCACCCGCTGACAAGGACGACCCCCATGCGCATCCCCCCATACTTCCCCCGTCTCGGCGCCCTCGCCCTGGCCTGCGCCCTGGCCCTGCCCGCGCAGGCGGCCGAGCGCGCCTGGAGCTACAGCTACaa                                                                         <  2:8529168/151‑1 (MQ=31)
                cGCAAGACCGGCGAGACCGTCACCACCCGCTGACAAGGACGACCCCCATGCGCATCCCCCCATACTTCCCCCGTCTCGGCGCCCTCGCCCTGGCCTGCGCCCGGGCCCTGCCCGCGCAGGCGGGCGCGGGCGCCGGGAGCTGCCGCTACaa                                                                         >  2:3165198/1‑151 (MQ=11)
                 gCAAGACCGGCGAGACCGTCACCACCCGCTGACAAGGACGACCCCCATGCGCATCCCCCCATACTTCCCCCGTCTCGGCGCCCGGGCCCTGGCCTGCGCCCTGGCCCGGCCCGCGCAGGCGGCCGAGCGCGCCGGGGGCGACAGCTACAAc                                                                        >  1:10048739/1‑151 (MQ=11)
                 gCAAGACCGGCGAGACCGTCACCACCCGCTGACAAGGACGACCCCCATGCGCATCCCCCCAGACTTCCCCCGTCTCGGCGCCCGCGGCCTGGCCTGCGCCCGGGCCCTGCCCGCGGAGGCGGCCGAGCGCGCCGGGAGCGACAGCTACCAc                                                                        >  2:5433256/1‑151 (MQ=11)
                  cAAGACCGGCGAGACCGTCACCACCCGCTGACAAGGACGACCCCCATGCGCATCCCCCCATACTTCCCCCGTCTCGGCGCCCTCGCCCTGGCCTGCGCCCTGGCCCTGCCCGCGCAGGCGGCCGAGCGCGCCTGGAGCTACAGCTACAACg                                                                       <  1:9891212/151‑1 (MQ=31)
                   aaGACCGGCGAGACCGTCACCACCCGCTGACAAGGACGACCCCCCTGCGCAACCCCCCCGCCGTCCCCCGGCTCGGGGCCCGCGCGGGGGCCTGCCCCCGGGCCCTGCCCGCGCGGGCGGGCGAGCGCGCCTGGAGCGACAGCGACAACGc                                                                      >  2:4674301/1‑151 (MQ=11)
                   aaGACCGGCGAGACCGTCACCACCCGCTGACAAGGACGACCCCCATGCGCATCCCCCCCTACTTCCCCCGTCTCGGCGCCCTCGCCCTGGCCTGCGCCCTGGCGCGGCCCGCGCAGGCGGCCGAGCGCGCCGGGAGGGGCAGCTACAACGc                                                                      >  2:7536693/1‑151 (MQ=11)
                   aaGACCGGCGAGACCGTCACCACCCGCTGACAAGGACGACCCCCATGCGCATCCCCCCATACTTCCCCCGTCTCGGCGCCCTCGCCCAGGCCTGCGCCCTGGCCCTGCCCGCGCAGGCGGCCGAGCGCGCCGGGGGCGCCAGCTACAACGc                                                                      >  2:8991174/1‑151 (MQ=255)
                   aaGACCGGCGAGACCGTCACCACCCGCTGACAAGGACGACCCCCATGCGCATCCCCCCAGACTTCCCCCGTCTCGGCGCCCGCGCCCAGGCCGGCGCCCGGGCCCGGCCCGCGCAGGCGGCCGAGCGCGCCTGGAGCGACAGCAACAACGc                                                                      >  2:11212113/1‑151 (MQ=11)
                   aaGACCGGCGAGACCGTCACCACCCGCGGACAAGGACGACCCCCATGCGCATCCCCCCATACTTCCCCCGTCTCGGCGCCCTCGCCCTGGCCTGCGCCCTGGCCCTGCCCGCGCAGGCGGCCGAGCGCGCCTGGAGCTACAGCTACAACGc                                                                      <  2:4658629/151‑1 (MQ=14)
                    aGACCGGCGAGACCGTCACCACCCGCTGACAAGGACGACCCCCATGCGCATCCCCCCATCCTTCCCCCGTCTCGGCGCCCGCGCCCAGGCCTGCGCCCGGGCCCTGCCCGCGCACGCGGCCGAGCGCGCCGGGAGCGCCAGCTACCACGcc                                                                     >  2:8772594/1‑151 (MQ=11)
                    aGACCGGCGAGACCGTCACCACCCGCTGACAAGGACGACCCCCATGCGCATCCCCCCATACTTCCCCCGTCTCGGCGCCCGCGCCCTGGCCTGCGCCCTGGCCCTGCCCGCGCCGGCGGCCGAGCGCGCCTGGAGCTACAGCTCCAACGcc                                                                     >  2:6415983/1‑151 (MQ=9)
                    aGACCGGCGAGACCGTCACCACCCGCTGACAAGGACGACCCCCATGCGCATCCCCCCATACTTCCCCCGTCTCGGCGCCCGCGCCCTGGCCTGCGCCCGGGCCCTGCCCGCGCAGGCGGCCGGGCGCGCCGGGAGCTACAGCGACAACGcc                                                                     >  2:808701/1‑151 (MQ=255)
                      aCCGGCGAGACCGTCACCACCCGCTGACAAGGACGACCCCCATGCGCATCCCCCCATACTTCCCCCGTCTCGGCGCCCTCGCCCTGGCCTGCGCCCTGGCCCTGCCCGCGCAGGCGGCCGAGCGCGCCTGGAGCTACAGCTACAACGCCCt                                                                   <  1:2570178/151‑1 (MQ=31)
                       ccGGCGAGACCGTCACCACCCGCTGACAAGGACGACCCCCATGCGCATCCCCCCATACTTCCCCCGTCTCGGCGCCCTCGCCCTGGCCTGCGCCCTGGCCCTGGCCGCGCAGGCGGCCGCGCGCGCCGGGAGCGACAGCTACAACGCCCTc                                                                  >  1:10091274/1‑151 (MQ=9)
                        cGGCGAGACCGTCACCACCCGCTGACAAGGACGACCCCCGTGCGCATCCCCCCATACTTCCCCCGTCTCGGCGCCCTCGCCCTGGCCGGCGCCCTGGCCCTTCCCGCGCAGGCGGCCGTGCGCGCCTGGAGCTCCGGCAGCCACGCCCTCg                                                                 >  2:2713848/1‑151 (MQ=11)
                        cGGCGAGACCGTCACCACCCGCTGACAAGGACGACCCCCATGCGCGTCCCCCCGGACGTCCCCCGGCTCGGCGCCCGCGCCCGGGCCTGCGCCCGGGCCCGGCCCGCGCGGGCGGCCGAGCGCGCCGGGCGGTCCAGCTACAACGCCCTCg                                                                 >  2:400649/1‑151 (MQ=11)
                        cGGCGAGACCGTCACCACCCGCTGACAAGGACGACCCCCATGCGCATCCCCCCATACTTCCCCCGTCTCGGCGCCCGCGCCCGGGCCTGCGCCCGGGCCCTGCCCGCGCCGGCGGCCCAGCGCGCCGGGGGCTACAGCTACACCGCCcgcg                                                                 >  1:6497424/1‑151 (MQ=11)
                         ggCGAGACCGTCACCACCCGCTGACAAGGACGACCCCCATGCGCATCCCCCCCTACTTCCCCCGTCTCGGCGCCCTCGGCCTGGCCTGCGCCCGGGCCCTGCCCGCGCCGGCGGCCGAGCCCGCCTGGAGCTACAGCTACAACGCCCTCgg                                                                >  2:10577528/1‑151 (MQ=255)
                         ggCGAGACCGTCACCACCCGCTGACAAGGACGACCCCCATGCGCATCCCCCCATACTTCCCCCGTCTCGGCGCCCTCGCCCTGGCCTGCGCCCTGGCCCTGCCCGCGCAGGCGGCCGAGCGCGCCTGGAGCTACAGCTACAACGCCCTCgg                                                                <  2:9074722/151‑1 (MQ=31)
                         ggCGAGACCGTCACCACCCGCTGACAAGGACGACCCCCATGCGCATCCCCCCATACTTCCCCCGTCTCGGCGCCCTCGCCCTGGCCTGCGCCCTGGCCCTGCCCGCGCAGGCGGCCGAGCGCGCCTGGAGCTACAGCTACAACGCCCTCgg                                                                <  2:9020579/151‑1 (MQ=31)
                         ggCGAGACCGTCACCACCCGCTGACAAGGACGACCCCCATGCGCATCCCCCCATACTTCCCCCGTCTCGGCGCCCTCGCCCTGGCCTGCGCCCTGGCCCTGCCCGCGCAGGCGGCCGAGCGCGCCTGGAGCTACAGCTACAACGCCCTCgg                                                                <  1:1921585/151‑1 (MQ=31)
                         ggCGAGACCGTCACCACCCGCTGACAAGGACGACCCCCATGCGCATCCCCCCATACTTCCCCCGTCTCGGCGCCCTCGCCCTGGCCTGCGCCCTGGCCCTGCCCGCGCAGGCGGCCGAGCGCGCCGGGAGCTACCGCTACAACGCCCTCgg                                                                >  2:7548183/1‑151 (MQ=14)
                         ggCGAGACCGTCACCACCCGCTGACAAGGACGACCCCCATGCGCATCCCCCCATACTTCCCCCGTCTCGGCGCCCGCGCCCGGGCCGGCGCCCAGGCCCTGCCCCCGCCGGCGGGCGAGCGGGCCAGGGGCGACAGCGGCAACGGCCGCgg                                                                >  1:2989126/1‑151 (MQ=11)
                           cGAGACCGTCACCACCCGCTGACAAGGACGACCCCCATGCGCATCCCCCCATACTTCCCCCGTCTCGGCGCCCTCGCCCTGGCCTGCGCCCTGGCCCTGCCCGCGCAGGCGGCCGAGCGCGCCTGGAGCTACAGCTACAACGCCCTCGGcc                                                              <  1:273515/151‑1 (MQ=31)
                            gagaCCGTCACCACCCGCTGACAAGGACGACCCCCATGCGCATCCCCCCATACTTCCCCCGTCTCGGCGCCCTCGCCCTGGCCTGCGCCCTGGCCCTGCCCGCGCAGGCGGCCGAGCGCGCCTGGAGCTACAGCTACAACGCCCTCGGCCt                                                             <  1:10835985/151‑1 (MQ=31)
                            gagaCCGTCACCACCCGCTGACAAGGACGACCCCCATGCGCATCCCCCCATACTTCCCCCGTCTCGGCGCCCTCGCCCTGGCCAGCGCCCTGGCCCTGCCCGCGCAGGCGGCCGAGCGCGCCTGGAGCTACAGCTACAACGCCCTCGGCCt                                                             <  1:1817571/151‑1 (MQ=14)
                            gagaCCGTCACCACCCGCTGACAAGGACGACCCCCATGCGCATCCCCCCATACTTCCCCCGTCTCGGCGCCCTCGCCCAGGCCTGCGCCCTGGCCCTGCCCGCGCAGGCGGCCGAGCGCGCCGGGAGCGAAAGCGACAACGCCCGCGGCCt                                                             >  1:2284869/1‑151 (MQ=11)
                            gagaCCGTCACCACCCGCTGACAAGGACGACCCCCATGCGCATCCCCCCATACTTCCCCCGTCTCGGCGCCCGCGCCCGGGCCGGCGCCCAGGCCCTGCCCGCCCAGGCGGCCGAGCGCGCCGGGAGCTACAACTCCAACGCCCGCGGCCt                                                             >  2:1947496/1‑151 (MQ=11)
                            gagaCCGTCACCACCCGCTGACAAGGACGACCCCCATGCGCATCCCCCCATACTTCCCCCGTCTCGGCGCCCGCGCCCAGGCCTGCGCCCGGGCCCTGCCCGCGCAGGCGGCCGCGCGCGCCGGGAGCGCCCGCGACCACGCCCGCGGccg                                                             >  2:8796620/1‑150 (MQ=11)
                             agaCCGTCACCACCCGCTGACAAGGACGACCCCCCTGCGCATCCCCCCATACTTCCCCCGTCTCGGCGCCCTCGCCCTGGCCTGCGCCCTGGCCCTGCCCGCGCAGGCGGCCGTGCGCGCCTGGAGCTACAGCTACAACGCCCTCGGCCTg                                                            <  1:6821793/151‑1 (MQ=14)
                             agaCCGTCACCACCCGCTGACAAGGACGACCCCCATGCGCATCCCCCCATACTTCCCCCGTCTCGGCGCCCTCGCCCTGGCCTGCGCCCTGGCCCTGCCCGCGCAGGCGGCCGAGCGCGCCTGGAGCTACAGCTACAACGCCCTCGGCCTg                                                            <  1:4207678/151‑1 (MQ=31)
                              gaCCGTCACCACCCGCTGACAAGGACGACCCCCATGCGCATCCCCCCATACTTCCCCCGTCTCGGCGCCCTCGCCCTGGCCTGCGCCCTGGCCCTGCCCGCGCAGGCGGCCGAGCGCGCCTGGAGCTACAGCTACAACGCCCTCGGCCTGa                                                           <  1:533523/151‑1 (MQ=31)
                               aCCGTCACCACCCGCTGACAAGGACGACCCCCATGCGCATCCCCCCATACTTCCCCCGTCTCGGCGCCCTCGCCCTGGCCTGCGCCCTGGCCCTGCCCGCGCAGGGGGCCGCGCGCGCCGGGGGGGGCAGCTACAACGCCCGCGGccggct                                                          >  2:9555257/1‑147 (MQ=11)
                               aCCGTCACCACCCGCTGACAAGGACGACCCCCATGCGCATCCCCCCATACTTCCCCCGTCTCGGCGCCCGCGCGCTGGCCGGGGCCCGGGCCCGGGCCGCGCAGGGCGCGGGGCGCGCCGGGAGCTCCCGCTGCACCGCCCGCGGCCAGag                                                          >  1:4016526/1‑150 (MQ=11)
                               aCCGTCACCACCCGCTGACAAGGACGACCCCCATGCGCATCCCCCCATACTTCCCCCGTCTCGGCGCCCGCGCCCGGGCCGGCGCCCTGGCCCAGCCCGCGCAGGCGGCCGAGCGCGCCGGGAGCGACCGCGACCACGCCCTCGGCCGGaa                                                          >  1:6520576/1‑150 (MQ=11)
                               aCCGTCACCACCCGCAGACAAGGACGACCCCCATGCGCATCCCCCCAGACTTCCCCCGGCTCGGCGCCCTCGCCCTGGCCTGCGCCCTGGCCCAGCCCGCGCAGGCGGCCGAGCGCGCCTGGAGCTACAGCTACAACGCCCTCGGCCTGAt                                                          <  2:6276547/151‑1 (MQ=9)
                                ccGTCACCACCCGCTGACAAGGACGACCCCCATGCGCATCCCCCCATACTTCCCCCGTCTCGGCGCCCTCGCCCTGGCCTGCGCCCTGGCCCTGCCCGCGCAGGCGGCCGAGCGCGCCTGGAGCTACAGCTACAACGCCCTCGGCCTGATc                                                         <  2:2591327/151‑1 (MQ=31)
                                ccGTCACCACCCGCTGACAAGGACGACCCCCATGCGCATCCCCCCATACTTCCCCCGTCTCGGCGCCCTCGCCCTGGCCAGCGCCCGGGGCCGGCCCGCGCCGGCGGCCGCGCCCGCCGGGAGCGCCAGCGAAAACGCCCGCGGCCGGATc                                                         >  1:5882350/1‑151 (MQ=11)
                                 cGTCACCACCCGCTGACACGGACGACCCCCATGCGCATCCCCCCATACTTCCCCCGTCTCGGCGCCCACGCCCAGGCCGGCGCCCTGGCCCGGCCCGCGGAGGCCGCCGAGCGCGCCGGGAGCGACCGCGACAACGCCCTCGGCCGGCTCg                                                        >  1:10730574/1‑151 (MQ=11)
                                    caccacCCGCTGACAAGGACGACCCCCATGCGCATCCCCCCATACTTCCCCCGTCTCGGCGGCCGCGCCCTGGCCGGCGCCCAGGCCCAGCCCGCGCCGGCGGCCGCGCGCGCCAGGTGCTACAGCGACCACGCCCGCGGGCGGATCGAgc                                                     >  1:4863970/1‑151 (MQ=11)
                                     accaccCGCTGACAAGGACGACCCCCATGCGCATCCCCCCATACTTCCCCCGTCTCGGCGCCCTCGCCCTGGCCTGCGCCCGGGCCCTGGCCGCGCAGGCGGCCGCGCGCGCCTGGAGCGACAGCGACAACGCCCTCGGCCGGATCGAgcg                                                    >  1:6650105/1‑151 (MQ=11)
                                     accaccCGCTGACAAGGACGACCCCCATGCGCATCCCCCCATACTTCCCCCGTCTCGGCGCCCGCGCCCAGGCCTGCGCCCTGGCCCTGCCCGCGCAGGCGGCCGAGCGCGCCGGGAGCTACAGCTACAACGCCCTCGGCCTGATCGAgcg                                                    >  2:5648183/1‑151 (MQ=9)
                                      ccaccCGCTGACAAGGACGACCCCCATGCGCATCCCCCCATACTTCCCCCGTCTCGGCGCCCTCGCCCTGGCCTGCGCCCTGGCCCTGCCCGCGCAGGCGGCCGAGCGCGCCTGGAGCTACAGCTACAACGCCCTCGGCCTGATCGAgcgc                                                   <  1:9786506/151‑1 (MQ=31)
                                      ccaccCGCTGACAAGGACGACCCCCATGCGCATCCCCCCATACTTCCCCCGTCTCGGCGCCCGCGCCCGGGCCTGCGCCCAGGCCCGGCCCGCGCCGGCGGCCGAGCGCCCCCGGAGCTACAGCTACAACGCCCGCGGCCGGATCGGgcgc                                                   >  1:2468050/1‑151 (MQ=11)
                                       caccCGCTGACAAGGACGACCCCCATGCGCATCCCCCCATACTTCCCCCGTCTCGGCGCCCGCGCCCAGGCCTGCGCCCGGGCCCTGCCCGCGCCGGCGGCCGAGCGCGCCCGGAGGAACAGCGCCAACCCCCACGGCCGGATcgcgcgcg                                                  >  1:5733087/1‑151 (MQ=11)
                                        accCGCTGACAAGGACGACCCCCATGCGCATCCCCCCATACTTCCCCCGTCTCGGCGCCCTCGCCCTGGCCTGCGCCCTGGCCCTGCCCGCGCAGGCGGCCGAGCGCGCCTGGAGCTACAGCTACAACGCCCTCGGCCTGATCGAgcgcgc                                                 <  1:6092465/151‑1 (MQ=31)
                                        accCGCTGACAAGGACGACCCCCATGCGCATCCCCCCATACTTCCCCCGTCTCGGCGCCCTCGCCCGGGCCTGCGCCCAGGCCCTGCCCGCGCAGGCGGCCGAGCGCGCCGGGAGCTACAGCGACAAAGCCCGCGGCCTGATAGAgcgcgc                                                 >  1:8296256/1‑151 (MQ=11)
                                        accCGCTGACAAGGACGACCCCCATGCGCATCCCCCCAGACTTCCCCCGTCTCGGCGCCCTCGCCCTGGCCAGCGCCCTGGCCCGGCCCGCGCAGGCGGCCGAGCGCGCCAGGAGCTACAGCTACAACGCCCACGGCCAGATCGAgcgcgc                                                 >  2:5301697/1‑151 (MQ=11)
                                         cccGCTGACAAGGACGACCCCCATGCGCATCCCCCCATACTTCCCCCGTCTCGGCGCCCTCGCCCTGGCCTGCGCCCTGGCCCTGCCCGCGCAGGCGGCCGAGCGCGCCGGGCGCTCCAGCTACAACGCCCTCGCCCTTCTCGAGCCCGcc                                                >  2:10687116/1‑151 (MQ=11)
                                          ccGCTGACAAGGACGACCCCCATGCGCATCCCCCCATACTTCCCCCGTCTCGGCGCCCTCGCCCTGGCCTGCGCCCTGGCCCTGCCCGCGCAGGCGGCCGAGCGCGCCAGGAGCTACAGCTACAACGCCCTCGGCCTGATCGAGCGCGCCg                                               >  2:7977907/1‑151 (MQ=14)
                                           cGCTGACAAGGACGACCCCCATGCGCATCCCCCCATACTTCCCCCGTCTCGGCGCCCTCGCCCGGGCCTGCGCCCTGGCCCTGCCCGCGCAGGCGGCCGAGCGCGCCTGGCGCTACAGCTCCAACGCCCTCGGCCGGATCGAGCGCGCCGa                                              >  2:9210026/1‑151 (MQ=9)
                                            gCTGACAAGGACGACCCCCATGCGCATCCCCCCCTACTTCCCCCGTCTCGGCGCCCTCGCCCTGGCCTGCGCCCTGGCCCTGCCCGCGCAGGCGGCCGAGCGCGCCTGGAGCTACAGCTACAACGCCCTCGGCCTGATCGAGCGCGCCGAc                                             <  1:2095899/151‑1 (MQ=14)
                                            gCTGACAAGGACGACCCCCATGCGCATCCCCCCATACTTCCCCCGTCTCGGCGCCCTCGCCCTGGCCTGCGCCCTGGCCCTGCCCGCGCAGGCGGCCGAGCGCGCCTGGAGCTACAGCTACAACGCCCTCGGCCTGATCGAGCGCGCCGAc                                             <  1:2086479/151‑1 (MQ=31)
                                            gCTGACAAGGACGACCCCCATGCGCATCCCCCCATACTTCCCCCGTCTCGGCGCCCTCGCCCTGGCCTGCGCCCTGGCCCTGCCCGCGCAGGCGGCCGAGCGCGCCTGGAGCTACAGCTACAACGCCCTCGGCCTGATCGAGCGCGCCGAc                                             <  1:5556798/151‑1 (MQ=31)
                                            gCTGACAAGGACGACCCCCATGCGCATCCCCCCATACTTCCCCCGTCTCGGCGCCCACGCCCAGGCCTGCGCCCAGGCCCTGCCCGCGCAGGCGGCCGAGCGCGCCAGGAGCTACAGCTACAACGCCCGCGGCCTGATAGAGCGCCCCGAc                                             >  1:2334942/1‑151 (MQ=11)
                                            ccTGACAACGACGACCCCCATGCGCAACCCCCCCGACGACCCCCGTCTCGGCGCCCTCGCCCGGCCCCGCGCCCAGGCCCTGCCCGCGCAGGCGGCCGAGCGCGCCTGGAGCTACAGCCACAACGCCCTCGGCCTGATCGAGCGCGCCgcc                                             <  2:1419108/150‑3 (MQ=11)
                                             cTGACAAGGACGACCCCCATGCGCATCCCCCCATACTTCCCCCGTCTCGGCGCCCTCGCCCTGGCCTGCGCCCTGGCCCTGCCCGCGCAGGCGGCCGAGCGCGCCTGGAGCTACAGCTACAACGCCCTCGGCCTGATCGAGCGCGCCGACg                                            <  2:2248840/151‑1 (MQ=31)
                                              tGACAAGGACGACCCCCATGCGCATCCCCCCCTACTTCCCCCGTCTCGGCGCCCTCGCCCTGGCCTGCGCCCTGGCCCTGCGCGCGCAGGCGGCCGAGCGCGCCTGGAGCTACAGCTACAACGCCCTCGGCCTGATCGAGCGCGCCGACgg                                           <  1:9269017/151‑1 (MQ=14)
                                              tGACAAGGACGACCCCCATGCGCATCCCCCCATACTTCCCCCGTCTCGGCGCCCTCGCCCTGGCCTGCGCCCTGGCCCTGCCCGCGCAGGCGGCCGAGCGCGCCTGGAGCTACAGCTACAACGCCCTCGGCCTGATCGAGCGCGCCGACgg                                           <  1:7057890/151‑1 (MQ=31)
                                              tGACAAGGACGACCCCCATGCGCATCCCCCCATACTTCCCCCGTCTCGGCGCCCTCGCCCTGGCCGGCGCCCGGGCCCTGCCCGCGCCGGCGGCCGAGCGCGCCGGGAGCTACAGCGACCACGCCCGCGGCCTGTTCGAGCGCGCCGCCgg                                           >  2:5834593/1‑151 (MQ=11)
                                               gACAAGGACGACCCCCATGCGCATCCCCCCATACTTCCCCCGTCTCGGCGCCCTCGCCCTGGCCTGCGCCCTGGCCCTGCCCGCGCAGGCGGCCGAGCGCGCCTGGAGCTACAGCTACAACGCCCTCGGCCTGATCGAGCGCGCCGACGGt                                          <  1:6130337/151‑1 (MQ=31)
                                               gACAAGGACGACCCCCATGCGCATCCCCCCATACTTCCCCCGTCTCGGCGCCCGCGCCCAGGCCAGCGCCCAGGCCCAGCCCGCGCAGGCGGCCCAGCGCGCCAGGAGCTACAGCTACAACGCCCTCGGCCTGCTCGAGCGCGCCGACGGt                                          >  1:8065345/1‑151 (MQ=11)
                                                aCAAGGACGACCCCCATGCGCATCCCCCCATACTTCCCCCGTCTCGGCGCCCTCGCCCTGGCCTGCGCCCTGGCCCTGCCCGCGCAGGCGGCCGAGCGCGCCTGGAGCTACAGCTACAACGCCCTCGGCCTGATCGAGCGCGCCGACGGTc                                         <  1:2465983/151‑1 (MQ=31)
                                                aCAAGGACGACCCCCATGCGCATCCCCCCATACTTCCCCCGTCTCGGCGCCCTCGCCCTGGCCTGCGCCCTGGCCCTGCCCGCGCAGGCGGCCGAGCGCGCCTGGAGCTACAGCTACAACGCCCTCGGCCTGATCGAGCGCGCCGACGGTc                                         <  1:3127891/151‑1 (MQ=31)
                                                 cAAGGACGACCCCCATGCGCATCCCCCCATACTTCCCCCGTCTCGGCGCCCTCGCCCTGGCCTGGGCCCTGGCCCTGGCCGCGCAGGCGGCCGAGCGCGCCTGGAGGTACAGCTACCACGCCCGCGGGCGGAACGGGCGCGCCGGCGGTcc                                        >  1:3217313/1‑151 (MQ=11)
                                                  aaGGACGACCCCCATGCGCATCCCCCCATACTTCCCCCGTCTCGGCGCCCTCGCCCTGGCCTGCGCCCTGGCCCTGCCCGCGCAGGCGGCCGAGCGCGCCTGGAGCGACAGCTACAACGCCCACGGCCTGATCGCGCGCGCCGACGGTCcg                                       >  2:6652934/1‑151 (MQ=9)
                                                  aaGGACGACCCCCATGCGCATCCCCCCATACTTCCCCCGTCTCGGCGCCCTCGCCCTGGCCTGCGCCCGGGCCCGGCCCGCGCCGGCGGCCGAGCGCGCCTGGAGCTACAGCGACCACGCCCGCGGCCTGATCGAGCGCGCCGACGGTCcg                                       >  1:7590674/1‑151 (MQ=11)
                                                   aGGACGACCCCCATGCGCATCCCCCCATACTTCCCCCGTCTCGGCGCCCTCGCCCTGGCCTGCGCCCTGGCCCTGCCCGCGCAGGCGGCCGAGCGCGCCGGGAGCTACAGCTACAACGCCCTCGGCCAGATCGAGCGCGCCGACGGTCcgc                                      >  2:8145711/1‑151 (MQ=14)
                                                    ggACGACCCCCATGCGCATCCCCCCATACTTCCCCCGTCTCGGCGCCCCCGCCCGGGCCGGCGCCCGGGCCCAGCCCGCGCAGGCGGCCGCGCGCGCCCGGCGCTACAGCGCCAAACCCCACGGCCAGTTCGAGCGCGCCGACCGGCcgcg                                     >  1:3120828/1‑151 (MQ=11)
                                                     gacgacCCCCATGCGCATCCCCCCATACTTCCCCCGTCTCGGCGCCCTCGCCCTGGCCAGCGCCCTGGCCCAGCCCGCGCAGGCGGCCGAGCGCGCCGGGAGCTACACCTACAACGCCCACGGCCTGATAGAGCGCGCCCACGGTCCgcgc                                    >  2:4151002/1‑151 (MQ=11)
                                                     gacgacCCCCATGCGCATCCCCCCATACTTCCCCCGTCTCGGCGCCCACCCCCGGGCCTGCGCCCAGGCCCTGCCCGCGCAGGCGGCCGAGCGCGCCGGGAGCTACAGCTACAACGCCCACGCCCGGATCGAGAGAGCCGACGCTCCgcgc                                    >  1:958857/1‑151 (MQ=11)
                                                      acgacCCCCCTGCGCATCCCCCCCTCCTTCCCCCGTCTCGGCGCCCTCGCCCTGGCCTGCGCCCTGGCCCTGCCCGCGCAGGCGGCCCAGCGCGCCTGGAGCTACAGCTACAACGCCCTCGGCCTGATCGAGCGCGCCGACGGTCCGCGCa                                   <  2:11350207/151‑1 (MQ=9)
                                                        gacCCCCATGCGCATCCCCCCATACTTCCCCCGTCTCGGCGCCCCCCCCCAGGCCAGCGCCCTGGCCCAGCCCCCGCAGGCGGCCGAGCGCGCCAGGAGCGACAGCTACAACCCCCACGGCCTGATCGAGCGCGCCGACGGTCCGCGcccc                                 >  1:3301520/1‑151 (MQ=11)
                                                        gacCCCCATGCGCATCCCCCCAGACTTCCCCCGTCTCGGCGCCCTCGCCCTGGCCTGCGCCCTGGCCCTGCCCGCGCAGGCGGCCGAGCGCGCCTGGAGCTACAGCTACAACGCCCTCGGCCTGATCGAGCGCGCCGACGGTCCGCGCAcc                                 <  2:3594006/151‑1 (MQ=14)
                                                         ccccccAGGCGCATCCCCCCATACATCCCCCGTCTCGGCGCCCTCGCCCTGGCCTGCGCCCCGGCCCTGCCCGCGCAGGCGGCCGAGCGCGCCTGGAGCTACAGCTACACCGCCCTCGGCCTGATCGAGCGCGCCGACGGTCCGCGCACCg                                <  2:8365480/150‑1 (MQ=9)
                                                         acCCCCATGCGCATCCCCCCATACTTCCCCCGTCTCGGCGCCCGCGCCCAGGCCGGCGCCCAGGCCCTGCCCGCGCAGGCGGCCGAGCGCGCCAGGAGCTACAGCTACAAAGCCCACGGCCGGCTAGAGCGCGCCCCCCGTCCCCGCAccc                                >  1:4553943/1‑150 (MQ=11)
                                                         acCCCCATGCGCATCCCCCCATACTTCCCCCGTCTCGGCGCCCACCCCCGGGCCAGCGCCCAGGCCCAGCCCCCGCAGGCGGCCCAGCGCCCCAGGAGCGAAACCTACAACGCCCACCGCCAGATAGAGCGCGCCGACCGTCCGCGCAACg                                >  1:10311461/1‑151 (MQ=11)
                                                         acCCCCATGCGCATCCCCCCATACTTCCCCCGTCTCGGCCCCCCCGCCCAGGCCAGCGCCCAGGCCCAGCCCCCGCCGGCCGCCGAGCGCGCCAGGAGCTACACCAACAAACCCCACGGCCGGATCGAGCGCGCCCACCGTCCCCGccccc                                >  1:10558569/1‑150 (MQ=2)
                                                          cccccATGCGCATCCCCCCATACTTCCCCCGTCTCGGCGCCCTCGCCCGGGCCTGCGCCCTGGCCCTGCCCGCGCAGGCGGCCGAGCGCGCCTGGAGCTACAGCTACAACGCCCTCGGCCGGATCGAGCGCGCCGACGGTCCGCGCACCGa                               >  2:4530682/1‑151 (MQ=14)
                                                           ccccCTGCGCCTCCCCCCCTACGTCCCCCGGCTCGGCGCCCCCGCCCTGGCCTGCGCCCGGGCCCTGCCCGCGCAGGCGGCCGAGCGCGCCTGGAGCAACAGCTACAACGCCCTCGGCCTGATCGAGCGCGCCGACGGTCCGCGCACCGAt                              <  1:7936728/151‑1 (MQ=11)
                                                           ccccATGCGCATCCCCCCATACTTCCCCCGTCTCGGCGCCCTCGCCCTGGCCTGCGCCCTGGCCCTGCCCGCGCAGGCGGCCGAGCGCGCCTGGAGCTACAGCTACAACGCCCTCGGCCTGATCGAGCGCGCCGACGGTCCGCGCACCGAt                              <  1:5904008/151‑1 (MQ=11)
                                                           ccccATGCGCATCCCCCCATACTTCCCCCGTCTCGGCGCCCGCGCCCTGGCCTGCGCCCGGGCCCTGCCCGCGCACGCGGCCGAGCGCGCCTGGAGCTACCGCTACCACGCCCACGGCCAGCACGAGCGCGCCGCCGGTGCGCGCACCgcg                              >  1:722779/1‑149 (MQ=11)
                                                             ccctGCGCATCCCCCCATACGACCCCCGGCTCGGCGCCCTCGCCCTGGCCTGCGCCCTGGCCCGGCCCGCGCAGGCGGCCGAGCGCGCCTGGAGCTACAGCTACAACGCCCTCGGCCTGCTCGAGCGCGCCGACGGTCCGCGCACCGAtgt                            <  2:3240615/148‑1 (MQ=1)
                                                                    cagccccccATCCTTCCCCCGTCTCGGCGCCCTCGCCCTGGCCTGCGCCCTGGCCCTGCCCGCGCAGGCGGCCGAGCGCGCCTGGAGCTACAGCTACAACGCCCTCGGCCTGATCGAGCGCGCCGACGGTCCGCGCACCGATGTGCAGGAc                     <  2:7112497/148‑1 (MQ=1)
                                                                    cagccccccATCCTTCCCCCGTCTCGGCGCCCTCGCCCCGGCCTGCGCCCTGGCCCTGCCCGCGCAGGCGGCCGAGCGCGCCTGGAGCTACAGCTACAACGCCCTCGGCCTGATCGAGCGCGCCGACGGTCCGCGCACCGATGTGCAGGAc                     <  2:10801214/148‑1 (MQ=0)
                                                                    cagccccccATACGGCCCCCGTCACGGCGCCCTCGCCCGGGCCTGCGCCCTGGCCCTGCCCGCGCCGGCGGCCGAGCGCGCCTGGAGCTACAGCTCCAACGCCCGCGGCCGGATCGAGCGCGCCGACCGTCCGCGCACCGATGTGCAGGAc                     <  2:10910423/148‑1 (MQ=1)
                                                                               acgtCCCCCGTCTCGGCGCCCTCGCCCTGGCCTGCGCCCTGGCCCTGCCCGCGCAGGCGGCCGAGCGCGCCTGGAGCTACAGCTACAACGCCCTCGGCCTGATCGAGCGCGCCGACGGTCCGCGCACCGATGTGCAGGACGTCACCCTCTa          <  1:8782113/148‑1 (MQ=1)
                                                                                                                                                      |                                                                                       
GTACGACGCCGAGGGACGCAAGACCGGCGAGACCGTCACCACCCGCTGACAAGGACGACCCCCATGCGCATCCCCCCATACTTCCCCCGTCTCGGCGCCCTCGCCCTGGCCTGCGCCCTGGCCCTGCCCGCGCAGGCGGCCGAGCGCGCCTGGAGCTACAGCTACAACGCCCTCGGCCTGATCGAGCGCGCCGACGGTCCGCGCACCGATGTGCAGGACGTCACCCTCTATGCCTACG  >  NC_008463/1763434‑1763671

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑