Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 2,807,266 G→C V137L (GTG→CTG)  PA14_RS13175 → leucine‑rich repeat‑containing protein kinase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084632,807,2660GC99.8% 2226.9 / NA 565V137L (GTG→CTG) PA14_RS13175leucine‑rich repeat‑containing protein kinase family protein
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base C (285/279);  total (286/279)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 580 total aligned reads displayed.

GGCCGACTCCCTGGAGGTGCTCAACCTCAGCGGCAACGCCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGGTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAGGAACTGGGTCGCCGGCCGTTGCAGAAGCTGATGC  >  NC_008463/2807118‑2807413
                                                                                                                                                    |                                                                                                                                                   
  ccGACTCCCTGGAGGTGCTCAACCTCAGCGGCAACGCCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAg                                                                                                                                                 >  1:8626952/1‑151 (MQ=255)
   cGACTCCCTGGAGGTGCTCAACCTCAGCGGCAACGCCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGc                                                                                                                                                >  2:4966933/1‑151 (MQ=255)
    gACTCCCTGGAGGTGCTCAACCTCAGCGGCAACGCCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCa                                                                                                                                               >  1:9765785/1‑151 (MQ=255)
     aCTCCCTGGAGGTGCTCAACCTCAGCGGCAACGCCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCAt                                                                                                                                              >  2:10076673/1‑151 (MQ=255)
      cTCCCTGGAGGTGCTCAACCTCAGCGGCAACGCCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATg                                                                                                                                             <  2:11318079/151‑1 (MQ=255)
      cTCCCTGGAGGTGCTCAACCTCAGCGGCAACGCCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATg                                                                                                                                             >  1:2533226/1‑151 (MQ=255)
        cccTGGAGGTGCTCAACCTCAGCGGCAACGCCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGt                                                                                                                                           <  1:10000286/151‑1 (MQ=255)
        cccTGGAGGTGCTCAACCTCAGCGGCAACGCCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGt                                                                                                                                           >  2:10238686/1‑151 (MQ=255)
         ccTGGAGGTGCTCAACCTCAGCGGCAACGCCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCCTGGTc                                                                                                                                          >  2:6665739/1‑151 (MQ=255)
            ggAGGTGCTCAACCTCAGCGGCAACGCCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGc                                                                                                                                       >  2:8392901/1‑151 (MQ=255)
             gAGGTGCTCAACCTCAGCGGCAACGCCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCt                                                                                                                                      <  2:6971635/151‑1 (MQ=255)
               ggTGCTCAACCTCAGCGGCAACGCCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTc                                                                                                                                    <  2:7648158/151‑1 (MQ=255)
                gTGCTCAACCTCAGCGGCAACGCCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCa                                                                                                                                   <  2:210829/151‑1 (MQ=255)
                 tGCTCAACCTCAGCGGCAACGCCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCaa                                                                                                                                  <  2:8102565/151‑1 (MQ=255)
                 tGCTCAACCTCAGCGGCAACGCCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCaa                                                                                                                                  >  1:7195092/1‑151 (MQ=255)
                   cTCAACCTCAGCGGCAACGCCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGt                                                                                                                                <  1:4077846/151‑1 (MQ=255)
                    tCAACCTCAGCGGCAACGCCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTc                                                                                                                               >  1:5026652/1‑151 (MQ=255)
                     cAACCTCAGCGGCAACGCCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCCTGGTCGGCTTCAAGTcc                                                                                                                              >  2:9437981/1‑151 (MQ=255)
                      aaCCTCAGCGGCAACGCCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCa                                                                                                                             <  1:5781576/151‑1 (MQ=255)
                       aCCTCAGCGGCAACGCCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCaa                                                                                                                            >  2:8642638/1‑151 (MQ=255)
                       aCCTCAGCGGCAACGCCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCaa                                                                                                                            >  1:1215587/1‑151 (MQ=255)
                          tCAGCGGCCACGCCCTGGACAGCCAGCCGGACGACCTCGGCCGGCTGCAACGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGTATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCg                                                                                                                         <  2:1823040/151‑1 (MQ=255)
                           cAGCGGCAACGCCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGc                                                                                                                        >  1:2908795/1‑151 (MQ=255)
                              cGGCAACGCCCTGGACCGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATc                                                                                                                     >  2:6437367/1‑151 (MQ=255)
                               ggCAACGCCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGTATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCCTGGTCGGCTTCAAGTCCAACCGCATCg                                                                                                                    >  2:10860199/1‑151 (MQ=255)
                                 cAACGCCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAg                                                                                                                  <  1:613237/151‑1 (MQ=255)
                                 cAACGCCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTACGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCTCTGAGCATGGTCGGCTTCAATTCCAACCGCATCGAg                                                                                                                  >  2:38067/1‑151 (MQ=255)
                                       ccTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTg                                                                                                            >  1:3625774/1‑151 (MQ=255)
                                          ggACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTgccg                                                                                                         >  1:9338183/1‑151 (MQ=255)
                                             cAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTgccggcc                                                                                                      <  1:4345359/151‑1 (MQ=255)
                                              aGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTgccggccg                                                                                                     >  1:8971045/1‑151 (MQ=255)
                                              aGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTgccggccg                                                                                                     <  2:8797782/151‑1 (MQ=255)
                                                 cTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCgg                                                                                                  <  2:2507367/151‑1 (MQ=255)
                                                        acgacCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTgcc                                                                                           >  1:10497728/1‑151 (MQ=255)
                                                         cgacCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTgccg                                                                                          >  2:6795984/1‑151 (MQ=255)
                                                              tCGGCCGGCTGCATCGTCTCAAGGTGCTGGTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGc                                                                                     >  2:910035/1‑151 (MQ=255)
                                                               cggccggcTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCg                                                                                    <  1:8115136/151‑1 (MQ=255)
                                                                ggccggcTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGt                                                                                   >  2:7081488/1‑151 (MQ=255)
                                                                 gccggcTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGtt                                                                                  >  1:7410987/1‑151 (MQ=255)
                                                                     gcTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTgcgc                                                                              <  1:9033771/151‑1 (MQ=255)
                                                                       tGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCgctg                                                                            >  1:7595729/1‑151 (MQ=255)
                                                                       tGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCgctg                                                                            >  1:201177/1‑151 (MQ=255)
                                                                       tGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCgctg                                                                            >  1:6923912/1‑151 (MQ=255)
                                                                       tGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCgctg                                                                            >  1:10221000/1‑151 (MQ=255)
                                                                         cATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCgctggc                                                                          >  2:6124249/1‑151 (MQ=255)
                                                                                  aaGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCa                                                                 >  1:9496770/1‑151 (MQ=255)
                                                                                  aaGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCa                                                                 >  1:6724198/1‑151 (MQ=255)
                                                                                  aaGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCa                                                                 >  1:1098120/1‑151 (MQ=255)
                                                                                    ggTGCTGTTCTGTTCCGCCACCGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCAcc                                                               <  2:225088/151‑1 (MQ=255)
                                                                                     gTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCg                                                              <  2:4593142/151‑1 (MQ=255)
                                                                                      tGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGa                                                             <  2:2603892/151‑1 (MQ=255)
                                                                                       gCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGAc                                                            <  2:5844635/151‑1 (MQ=255)
                                                                                       gCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGAc                                                            <  1:209752/151‑1 (MQ=255)
                                                                                          gttctgttcCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAAc                                                         >  1:8169805/1‑151 (MQ=255)
                                                                                            tctgttcCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCg                                                       <  1:10413651/151‑1 (MQ=255)
                                                                                             ctgttcCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCgg                                                      >  1:2255744/1‑151 (MQ=255)
                                                                                               gttcCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGAt                                                    >  1:8406773/1‑151 (MQ=255)
                                                                                                ttcCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGAtt                                                   <  2:9831149/151‑1 (MQ=255)
                                                                                                 tcCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAAGCGGATTg                                                  >  2:11133811/1‑151 (MQ=255)
                                                                                                  ccGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGc                                                 <  1:3296576/151‑1 (MQ=255)
                                                                                                   cGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGcc                                                >  1:7952109/1‑151 (MQ=255)
                                                                                                    gCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACACCCGGATTGCCa                                               >  1:3575340/1‑151 (MQ=255)
                                                                                                    gCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCa                                               >  2:1572403/1‑151 (MQ=255)
                                                                                                     ccAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCAc                                              <  1:11089966/151‑1 (MQ=255)
                                                                                                       aaCGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGc                                            >  1:2399223/1‑151 (MQ=255)
                                                                                                        aCGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCt                                           <  1:1431342/151‑1 (MQ=255)
                                                                                                        aCGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCt                                           >  2:3435520/1‑151 (MQ=255)
                                                                                                          gACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGc                                         >  2:3637339/1‑151 (MQ=255)
                                                                                                            cTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGccc                                       >  2:4063904/1‑151 (MQ=255)
                                                                                                             ttCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACACCCGGATTGCCACGCTGCCCg                                      >  1:3809956/1‑151 (MQ=255)
                                                                                                              tCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCACGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGa                                     <  2:4382506/151‑1 (MQ=255)
                                                                                                              tCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGa                                     <  1:11193449/151‑1 (MQ=255)
                                                                                                               cGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAg                                    <  1:10097488/151‑1 (MQ=255)
                                                                                                                 ccGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAGGa                                  <  2:5443311/151‑1 (MQ=255)
                                                                                                                   gAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAGGAAc                                >  2:1316893/1‑151 (MQ=255)
                                                                                                                     aTTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAGGAACTg                              <  2:8484197/151‑1 (MQ=255)
                                                                                                                     aTTGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAGGAACTg                              >  1:1641989/1‑151 (MQ=255)
                                                                                                                      ttGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAGGAACTgg                             <  2:3351842/151‑1 (MQ=255)
                                                                                                                      ttGCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAGGAACTgg                             <  2:7851230/151‑1 (MQ=255)
                                                                                                                        gCCGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAGGAACTGGGt                           <  2:5075356/151‑1 (MQ=255)
                                                                                                                         ccGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAGGAACTGGGTc                          <  1:879451/151‑1 (MQ=255)
                                                                                                                         ccGGCGGCGGTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAGGAACTGGGTc                          <  2:2771709/151‑1 (MQ=255)
                                                                                                                           ggcggcggTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAGGAACTGGGTCgc                        >  1:6346747/1‑151 (MQ=255)
                                                                                                                              ggcggTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAGGAACTGGGTCgccgg                     <  2:5757807/151‑1 (MQ=255)
                                                                                                                               gcggTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAGGAACTGGGTCgccggc                    >  2:2678879/1‑151 (MQ=255)
                                                                                                                                cggTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAGGAACTGGGTCgccggcc                   >  1:5394674/1‑151 (MQ=255)
                                                                                                                                 ggTGGGCGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCTACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAGGAACTGGGTCgccggccg                  <  1:7739537/151‑1 (MQ=255)
                                                                                                                                       cGACTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAGGAACTGGGTCGCCGGCCGTTGCAg            <  1:2959297/151‑1 (MQ=255)
                                                                                                                                          cTGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAGGAACTGGGTCGCCGGCCGTTGCAGAAg         >  1:4592711/1‑151 (MQ=255)
                                                                                                                                           tGTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAGGAACTGGGTCGCCGGCCGTTGCAGAAGc        >  1:1491054/1‑151 (MQ=255)
                                                                                                                                            gTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAGGAACTGGGTCGCCGGCCGTTGCAGAAGCt       >  1:3720861/1‑151 (MQ=255)
                                                                                                                                            gTCCGGCGCTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAGGAACTGGGTCGCCGGCCGTTGCAGAAGCt       >  1:3180224/1‑151 (MQ=255)
                                                                                                                                               cggcgcTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAGGAACTGGGTCGCCGGCCGTTGCAGAAGCTGAt    <  2:2876041/151‑1 (MQ=255)
                                                                                                                                               cggcgcTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAGGAACTGGGTCGCCGGCCGTTGCAGAAGCTGAt    >  2:10345348/1‑151 (MQ=255)
                                                                                                                                                ggcgcTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAGGAACTGGGTCGCCGGCCGTTGCAGAAGCTGATg   <  1:5284510/151‑1 (MQ=255)
                                                                                                                                                ggcgcTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAGGAACTGGGTCGCCGGCCGTTGCAGAAGCTGATg   <  2:744116/151‑1 (MQ=255)
                                                                                                                                                ggcgcTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAGGAACTGGGTCGCCGGCCGTTGCAGAAGCTGATg   <  2:1691778/151‑1 (MQ=255)
                                                                                                                                                ggcgcTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAGGAACTGGGTCGCCGGCCGTTGCAGAAGCTGATg   <  2:4011951/151‑1 (MQ=255)
                                                                                                                                                 gcgcTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAGGAACTGGGTCGCCGGCCGTTGCAGAAGCTGATGc  >  1:10607086/1‑151 (MQ=255)
                                                                                                                                                 gcgcTGAGCATGGTCGGCTTAAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGATGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAGGAACTGGGTCGCCGGCCGTTGCAGAAGCTGATGc  <  2:7309307/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GGCCGACTCCCTGGAGGTGCTCAACCTCAGCGGCAACGCCCTGGACAGCCTGCCGGACGACCTCGGCCGGCTGCATCGTCTCAAGGTGCTGTTCTGTTCCGCCAACGACTTCGCCGAATTGCCGGCGGCGGTGGGCGACTGTCCGGCGGTGAGCATGGTCGGCTTCAAGTCCAACCGCATCGAGCGGGTGCCGGCCGCGGCCCTGCCGCCGGCGTTGCGCTGGCTGATCCTCACCGACAACCGGATTGCCACGCTGCCCGAGGAACTGGGTCGCCGGCCGTTGCAGAAGCTGATGC  >  NC_008463/2807118‑2807413

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑