Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 2,840,444 T→C intergenic (+44/‑45) PA14_RS13335 → / → PA14_RS13340 LysR substrate‑binding domain‑containing protein/sigma‑70 family RNA polymerase sigma factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084632,840,4440TC100.0% 2120.5 / NA 547intergenic (+44/‑45)PA14_RS13335/PA14_RS13340LysR substrate‑binding domain‑containing protein/sigma‑70 family RNA polymerase sigma factor
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (271/276);  total (271/276)

Only 100 of 547 total aligned reads displayed.

CCGGCGAGGGCGCGGTGGGTATGTGCTGGCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTTTTGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTGCAGAACTGGTTGCGCGGCAAGCTGG  >  NC_008463/2840297‑2840588
                                                                                                                                                   |                                                                                                                                                
ccGGCGAGGGCGCGGTGGGTATGTGCTGGCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGAc                                                                                                                                               <  2:9703540/151‑1 (MQ=255)
 cGGCGAGGGCGCGGTGGGTATGTGCTGGCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACa                                                                                                                                              <  2:5445185/151‑1 (MQ=255)
   gCGAGGGCGCGGTGGGTATGTGCTGGCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAAt                                                                                                                                            >  2:11095671/1‑151 (MQ=255)
   gCGAGGGCGCGGTGGGTATGTGCTGGCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAAt                                                                                                                                            <  1:11269162/151‑1 (MQ=255)
     gcgggCGCGGTGGGTATGTGCTGGCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACCATGc                                                                                                                                          <  2:154185/149‑1 (MQ=255)
     gAGGGCGCGGTGGGTATGTGCTGGCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGc                                                                                                                                          >  2:2297573/1‑151 (MQ=255)
       gggCGCGGTGGGTATGTGCTGGCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGa                                                                                                                                        <  2:3335571/151‑1 (MQ=255)
        ggCGCGGTGGGTATGTGCTGGCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGAt                                                                                                                                       >  2:10809290/1‑151 (MQ=255)
            cgGTGGGTATGTGCTGGCGCGACGACCCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTAGTGCCAAAAGGCCGCGGCTTCTCGACAATGCGATTCAt                                                                                                                                   >  2:5128591/1‑151 (MQ=255)
              gTGGGTATGTGCTGGCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTa                                                                                                                                 >  1:803714/1‑151 (MQ=255)
               tGGGTATGTGCTGGCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTAt                                                                                                                                <  2:10719763/151‑1 (MQ=255)
               tGGGTATGTGCTGGCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTAt                                                                                                                                >  2:4932457/1‑151 (MQ=255)
               tGGGTATGTGCTGGCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCAGTAt                                                                                                                                >  2:455361/1‑151 (MQ=255)
                   tATGTGCTGGCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTAtcatt                                                                                                                            >  2:10966426/1‑151 (MQ=255)
                   tATGTGCTGGCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTAtcatt                                                                                                                            >  2:58521/1‑151 (MQ=255)
                   tATGTGCTGGCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTAtcatt                                                                                                                            >  1:6857798/1‑151 (MQ=255)
                    aTGTGCTGGCGCGACGAACCGCGTCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTAtcattc                                                                                                                           <  2:6898154/151‑1 (MQ=255)
                    aTGTGCTGGCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTAtcattc                                                                                                                           >  2:7335127/1‑151 (MQ=255)
                    aTGTGCTGGCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTAtcattc                                                                                                                           <  2:1044282/151‑1 (MQ=255)
                     tgtgCTGGCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTAtcattca                                                                                                                          >  2:8468708/1‑151 (MQ=255)
                     tgtgCTGGCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGAATCATTAtcagtca                                                                                                                          >  2:5497847/1‑151 (MQ=255)
                         cTGGCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCAtgtg                                                                                                                      <  1:5720317/151‑1 (MQ=255)
                         cTGGCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCAtgtg                                                                                                                      >  1:1735968/1‑151 (MQ=255)
                           ggCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTc                                                                                                                    >  2:101168/1‑151 (MQ=255)
                           ggCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTc                                                                                                                    <  1:5580599/151‑1 (MQ=255)
                            gcgcGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCg                                                                                                                   <  1:8516860/151‑1 (MQ=255)
                               cgacgaACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGccc                                                                                                                >  1:2823001/1‑151 (MQ=255)
                                gacgaACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACATTGCGATTCATTATCATTCATGTGTCGcccc                                                                                                               >  2:7616567/1‑151 (MQ=255)
                                gacgaACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACACTGCGATTCATTATCATTCATGTGTCGcccc                                                                                                               <  2:7220333/151‑1 (MQ=255)
                                gacgaACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGcccc                                                                                                               >  1:3920556/1‑151 (MQ=255)
                                  cgaACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGa                                                                                                             >  2:11282562/1‑151 (MQ=255)
                                    aaCCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACt                                                                                                           <  2:3139807/151‑1 (MQ=255)
                                    aaCCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACt                                                                                                           >  1:5265747/1‑151 (MQ=255)
                                      ccGCCTCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACCATGCGATTCATTATCATTCATGTGTCGCCCCGACttt                                                                                                         <  2:9067375/151‑1 (MQ=255)
                                       cGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTc                                                                                                        <  2:8399581/151‑1 (MQ=255)
                                        gCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTcc                                                                                                       >  1:6740637/1‑151 (MQ=255)
                                            cgcgACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATg                                                                                                   <  1:9107593/151‑1 (MQ=255)
                                            cgcgACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATg                                                                                                   >  2:4803824/1‑151 (MQ=255)
                                             gcgACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGt                                                                                                  <  2:3639201/151‑1 (MQ=255)
                                               gACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTcc                                                                                                <  1:9276406/151‑1 (MQ=255)
                                                    cgcCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTAGTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGcccc                                                                                           <  2:6122623/151‑1 (MQ=255)
                                                      ccAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACACTGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGa                                                                                         <  2:1876785/151‑1 (MQ=255)
                                                         gCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATcc                                                                                      <  1:10326300/151‑1 (MQ=255)
                                                          cGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCt                                                                                     <  1:11057581/151‑1 (MQ=255)
                                                              gACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTtctc                                                                                 <  1:3146055/151‑1 (MQ=255)
                                                              gACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTtctc                                                                                 <  2:9343972/151‑1 (MQ=255)
                                                                ccTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCAGGTCCGCCCCGATCCTTCTCAg                                                                               >  2:10642266/1‑151 (MQ=255)
                                                                  tgctgGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAgcg                                                                             >  2:4397706/1‑151 (MQ=255)
                                                                   gctgGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAgcgc                                                                            >  2:9466814/1‑151 (MQ=255)
                                                                      ggCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCcca                                                                         >  2:2021005/1‑151 (MQ=255)
                                                                       gcctgcctGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACCATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCccac                                                                        <  1:3749113/151‑1 (MQ=255)
                                                                       gcctgcctGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCccac                                                                        >  2:6434826/1‑151 (MQ=255)
                                                                       gcctgcctGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCccac                                                                        >  1:5066967/1‑151 (MQ=255)
                                                                            cctgcGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATcg                                                                   >  2:9167255/1‑151 (MQ=255)
                                                                             ctgcGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAATGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATcgc                                                                  >  1:5846141/1‑151 (MQ=255)
                                                                             ctgcGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATcgc                                                                  <  2:9814917/151‑1 (MQ=255)
                                                                                   gAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCcatg                                                            >  2:565247/1‑151 (MQ=255)
                                                                                   gAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCcatg                                                            >  1:5206482/1‑151 (MQ=255)
                                                                                      ggCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCcatgcat                                                         <  1:8360489/151‑1 (MQ=255)
                                                                                        cgcgGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCatgcatgc                                                       >  1:9560751/1‑151 (MQ=255)
                                                                                        cgcgGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCatgcatgc                                                       <  2:78375/151‑1 (MQ=255)
                                                                                          cggcggCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATgcgc                                                     >  1:7630393/1‑151 (MQ=255)
                                                                                           ggcggcCGCGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCt                                                    >  1:10037401/1‑151 (MQ=255)
                                                                                              ggcCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTa                                                 >  1:10762600/1‑151 (MQ=255)
                                                                                               gcCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTAc                                                >  2:6248505/1‑151 (MQ=255)
                                                                                                ccGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACa                                               <  1:1912661/151‑1 (MQ=255)
                                                                                                 cGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAg                                              >  2:6773772/1‑151 (MQ=255)
                                                                                                  gAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGc                                             >  2:1288639/1‑151 (MQ=255)
                                                                                                  gAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGc                                             >  1:10629513/1‑151 (MQ=255)
                                                                                                    gTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGa                                           <  1:1816839/151‑1 (MQ=255)
                                                                                                      aGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGc                                         >  1:4008991/1‑151 (MQ=255)
                                                                                                        ggCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCAc                                       <  2:1721451/151‑1 (MQ=255)
                                                                                                            ccggccggCGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATg                                   <  1:10189233/151‑1 (MQ=255)
                                                                                                             cggccggcGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATgg                                  >  1:8636642/1‑151 (MQ=255)
                                                                                                              ggccggcGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGc                                 >  1:9037920/1‑151 (MQ=255)
                                                                                                              ggccggcGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGc                                 >  1:781796/1‑151 (MQ=255)
                                                                                                               gccggcGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCt                                <  2:8072826/151‑1 (MQ=255)
                                                                                                                ccggcGGGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTg                               <  1:4855835/151‑1 (MQ=255)
                                                                                                                 cggcggGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTgg                              >  1:10526854/1‑151 (MQ=255)
                                                                                                                 cggcggGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTgg                              >  1:1512720/1‑151 (MQ=255)
                                                                                                                    cggGTGCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGAGTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGCTCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCAGGGCTGGGTg                           <  2:2238587/151‑1 (MQ=255)
                                                                                                                      ggTGCTATAGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCGGTACAGCGAGCACCATGGCTGGTTGCa                         <  1:11458435/151‑1 (MQ=255)
                                                                                                                         gCTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTGCAGaa                      <  1:2973641/151‑1 (MQ=255)
                                                                                                                          cTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTGCAGAAc                     >  1:8171265/1‑151 (MQ=255)
                                                                                                                          cTATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTGCAGAAc                     >  2:297979/1‑151 (MQ=255)
                                                                                                                           tATTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTGCAGAACt                    >  2:5313737/1‑151 (MQ=255)
                                                                                                                            aTTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTGCAGAACTg                   >  1:1305086/1‑151 (MQ=255)
                                                                                                                            aTTGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTGCAGAACTg                   >  2:2696495/1‑151 (MQ=255)
                                                                                                                             ttGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTGCAGAACTgg                  <  1:2228976/151‑1 (MQ=255)
                                                                                                                              tGCAAAAAGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTGCAGAACTGGt                 <  1:4339480/151‑1 (MQ=255)
                                                                                                                                  aaaaGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTGCAGAACTGGTTgcg             >  1:3459290/1‑151 (MQ=255)
                                                                                                                                   aaaGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGGACAGCGAGCACCATGGCTGGTTGCAGAACTGGTTgcgc            <  2:8184331/151‑1 (MQ=255)
                                                                                                                                    aaGGCCGCGGCTTCTCGACAATGCGATTCATTGTCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTGCAGAACTGGTTGcgcg           >  2:4211081/1‑151 (MQ=255)
                                                                                                                                    aaGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTGCAGAACTGGTTGcgcg           <  1:9865124/151‑1 (MQ=255)
                                                                                                                                     aGGCCGCGGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTGCAGAACTGGTTGCGCgg          >  1:6160910/1‑151 (MQ=255)
                                                                                                                                         cgcgGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTGCAGAACTGGTTGCGCGGCAAg      >  1:4531765/1‑151 (MQ=255)
                                                                                                                                         cgcgGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTGCAGAACTGGTTGCGCGGCAAg      <  1:7201946/151‑1 (MQ=255)
                                                                                                                                           cgGCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTGCAGAACTGGTTGCGCGGCAAGCt    >  2:3998605/1‑151 (MQ=255)
                                                                                                                                            ggCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTGCAGAACTGGTTGCGCGGCAAGCTg   >  2:1023047/1‑151 (MQ=255)
                                                                                                                                            ggCTTCTCGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTGCAGAACTGGTTGCGCGGCAAGCTg   <  1:8816137/151‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
CCGGCGAGGGCGCGGTGGGTATGTGCTGGCGCGACGAACCGCATCGCGACCGCGCCAGCGAAGACCTGCTGGCCTGCCTGCGCGAAGGCGCGGCGGCCGAGTAGGGCGCCGGCCGGCGGGTGCTATTGCAAAAAGGCCGCGGCTTTTTGACAATGCGATTCATTATCATTCATGTGTCGCCCCGACTTTCCCATGTCCGCCCCGATCCTTCTCAGCGCCCACCATCGCGCCATGCATGCGCTGTACAGCGAGCACCATGGCTGGTTGCAGAACTGGTTGCGCGGCAAGCTGG  >  NC_008463/2840297‑2840588

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑