Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 2,955,433 A→G A288A (GCT→GCC)  PA14_RS13700 ← PepSY domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084632,955,4330AG99.8% 1796.4 / NA 457A288A (GCT→GCC) PA14_RS13700PepSY domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (256/200);  minor base C (0/1);  total (256/201)
Fisher's exact test for biased strand distribution p-value = 4.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 457 total aligned reads displayed.

CGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAAGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCAGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAAGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGCACGTCGGCG  >  NC_008463/2955285‑2955580
                                                                                                                                                    |                                                                                                                                                   
  ccgAAATCGCCGGTGTGCAGGGGCCGCATGGATTCGGGGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTGCGATCTTGCCGGTCGAAGGGTTCAGCGCGGCCGACTGGAACAGGAGCGGATACCAGCCGCGTCCGGCCa                                                                                                                                                 >  2:176793/1‑151 (MQ=255)
  ccgAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCa                                                                                                                                                 >  2:2858828/1‑151 (MQ=255)
  ccgAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCa                                                                                                                                                 <  1:4458584/151‑1 (MQ=255)
  ccgAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCa                                                                                                                                                 >  1:6759634/1‑151 (MQ=255)
    gAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACg                                                                                                                                               >  2:7208056/1‑151 (MQ=255)
         cGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACAt                                                                                                                                          <  1:8588460/151‑1 (MQ=255)
         cGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACAt                                                                                                                                          >  1:3057415/1‑151 (MQ=255)
          gCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATc                                                                                                                                         >  2:3752791/1‑151 (MQ=255)
          gCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATc                                                                                                                                         <  2:9433430/151‑1 (MQ=255)
           ccGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCg                                                                                                                                        >  1:250629/1‑151 (MQ=255)
           ccGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCg                                                                                                                                        <  2:6247765/151‑1 (MQ=255)
                 tgCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGt                                                                                                                                  >  2:2507740/1‑151 (MQ=255)
                    aGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAgg                                                                                                                               <  2:9214899/151‑1 (MQ=255)
                      gggACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCg                                                                                                                             <  1:4424932/151‑1 (MQ=255)
                             aTGGATTCGGTGACGAACTGCAGGGGGTTACGGTCGCTGAGCAGGCGGGTGGTGTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCgg                                                                                                                      >  1:1892282/1‑151 (MQ=255)
                             aTGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCgg                                                                                                                      <  2:4842848/151‑1 (MQ=255)
                              tGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCggg                                                                                                                     <  1:10299768/151‑1 (MQ=255)
                                  ttCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCagg                                                                                                                 >  2:5681801/1‑149 (MQ=255)
                                   tCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGa                                                                                                                >  1:4202787/1‑151 (MQ=255)
                                    cGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGaa                                                                                                               >  1:9958409/1‑151 (MQ=255)
                                     ggTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAac                                                                                                              <  2:10671712/151‑1 (MQ=255)
                                        gACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACAcc                                                                                                           <  1:2127824/151‑1 (MQ=255)
                                        gACGAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACAcc                                                                                                           >  2:7554685/1‑151 (MQ=255)
                                           gAACTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACcgcg                                                                                                        >  1:8471853/1‑151 (MQ=255)
                                            aaCTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCgcgc                                                                                                       <  1:5730056/151‑1 (MQ=255)
                                             aCTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCt                                                                                                      >  2:235475/1‑151 (MQ=255)
                                             aCTGCAGGGGGGTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCt                                                                                                      >  2:803661/1‑151 (MQ=255)
                                                   ggggggTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTc                                                                                                <  1:9027477/151‑1 (MQ=255)
                                                   ggggggTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGGAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTc                                                                                                >  2:4635612/1‑151 (MQ=255)
                                                    gggggTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTcc                                                                                               <  2:9699769/151‑1 (MQ=255)
                                                      gggTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAg                                                                                             <  2:9823936/151‑1 (MQ=255)
                                                       ggTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAgg                                                                                            >  2:4119241/1‑151 (MQ=255)
                                                        gTACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGc                                                                                           >  1:10018613/1‑151 (MQ=255)
                                                         tACGGTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCt                                                                                          <  2:7478384/151‑1 (MQ=255)
                                                             gTCGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTgggg                                                                                      >  1:6488112/1‑151 (MQ=255)
                                                               cGCTGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGAt                                                                                    <  2:10417952/151‑1 (MQ=255)
                                                                  tGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACg                                                                                 >  2:302961/1‑151 (MQ=255)
                                                                  tGAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACg                                                                                 >  1:11185339/1‑151 (MQ=255)
                                                                   gAGCAGGCGGGTTGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCTGACGACTGGAAAAAGAGCTGATACCAGCCGCTTCCGGCAACTAACATCTTGCTTAAGGCGTTTCCGGGCAGGACCACCTCTCTGGCCTCAAGGCTGGTGATACGc                                                                                >  2:608248/1‑151 (MQ=255)
                                                                   gAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGc                                                                                >  1:3491563/1‑151 (MQ=255)
                                                                   gAGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGc                                                                                >  1:8080009/1‑151 (MQ=255)
                                                                    aGCAGGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGcc                                                                               >  1:4044404/1‑151 (MQ=255)
                                                                        ggCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGt                                                                           >  2:3960680/2‑151 (MQ=255)
                                                                          cGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGa                                                                         <  2:6556701/151‑1 (MQ=255)
                                                                          cGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGa                                                                         <  2:10082648/151‑1 (MQ=255)
                                                                           gggTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGAc                                                                        >  2:6657554/1‑151 (MQ=255)
                                                                            ggtggtTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACg                                                                       <  1:7005006/151‑1 (MQ=255)
                                                                             gtggtTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGa                                                                      >  1:9640923/1‑151 (MQ=255)
                                                                              tggtTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAg                                                                     <  1:7061918/151‑1 (MQ=255)
                                                                               ggtTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGc                                                                    <  2:1525874/151‑1 (MQ=255)
                                                                                gtTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGcc                                                                   >  2:7687693/1‑151 (MQ=255)
                                                                                 tttCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCg                                                                  >  1:7381894/1‑151 (MQ=255)
                                                                                 tttCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCg                                                                  <  2:9156008/151‑1 (MQ=255)
                                                                                   tCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCa                                                                >  1:3171655/1‑151 (MQ=255)
                                                                                     gATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCAcc                                                              <  1:541015/151‑1 (MQ=255)
                                                                                      aTCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCg                                                             >  1:1018234/1‑151 (MQ=255)
                                                                                      aTCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCg                                                             >  1:11363787/1‑151 (MQ=255)
                                                                                      aTCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCg                                                             <  1:6227661/151‑1 (MQ=255)
                                                                                         ttGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCt                                                          <  2:4468746/151‑1 (MQ=255)
                                                                                          tGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTc                                                         <  1:7440814/151‑1 (MQ=255)
                                                                                            ccGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGt                                                       >  2:8801844/1‑151 (MQ=255)
                                                                                                 ataAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGc                                                  >  2:194248/1‑151 (MQ=255)
                                                                                                  taAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCt                                                 <  1:2277566/151‑1 (MQ=255)
                                                                                                  taAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCt                                                 <  2:3252846/151‑1 (MQ=255)
                                                                                                  taAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCt                                                 >  1:10849198/1‑151 (MQ=255)
                                                                                                    aGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCg                                               >  1:4559754/1‑151 (MQ=255)
                                                                                                        ttCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGt                                           <  2:5166660/151‑1 (MQ=255)
                                                                                                         tCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTa                                          >  2:831812/1‑151 (MQ=255)
                                                                                                          cAGCGCGGCCGACTGGACCATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTAt                                         <  2:2280118/151‑1 (MQ=255)
                                                                                                           aGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATg                                        <  1:275569/151‑1 (MQ=255)
                                                                                                            gcgcGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATgg                                       >  1:5083844/1‑151 (MQ=255)
                                                                                                            gcgcGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATgg                                       <  1:10788017/151‑1 (MQ=255)
                                                                                                            gcgcGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATgg                                       <  1:4768004/151‑1 (MQ=255)
                                                                                                              gcgGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGaa                                     <  2:11024983/151‑1 (MQ=255)
                                                                                                               cgGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAAc                                    >  1:5201320/1‑151 (MQ=255)
                                                                                                                    gACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCt                               <  2:7902326/151‑1 (MQ=255)
                                                                                                                    gACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCt                               >  1:4247366/1‑151 (MQ=255)
                                                                                                                    gACTGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCt                               >  1:7626358/1‑151 (MQ=255)
                                                                                                                       tGGAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTGGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGAc                            >  2:1435189/1‑151 (MQ=255)
                                                                                                                        ggAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGAcc                           <  1:1542554/151‑1 (MQ=255)
                                                                                                                         gAACATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCg                          >  1:7962049/1‑151 (MQ=255)
                                                                                                                          aaCATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGTCCgg                         >  2:4442192/1‑151 (MQ=255)
                                                                                                                           aCATGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCggg                        >  1:11053361/1‑151 (MQ=255)
                                                                                                                             aTGAGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTg                      <  1:2974306/151‑1 (MQ=255)
                                                                                                                                aGCGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTgcgg                   >  1:3356632/1‑151 (MQ=255)
                                                                                                                                  cGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTgcggcg                 <  1:10476814/151‑1 (MQ=255)
                                                                                                                                  cGGATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTgcggcg                 >  1:776000/1‑151 (MQ=255)
                                                                                                                                    gATACCAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGAGGTATGGAACCTTCTGACCGGGTGCGGcgcg               >  2:4183043/1‑151 (MQ=255)
                                                                                                                                    gATACCAGCCGCGTCCGGCCACCAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGCGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGcgcg               <  1:3756099/151‑1 (MQ=255)
                                                                                                                                        ccAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGc           >  2:1163884/1‑151 (MQ=255)
                                                                                                                                         cAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGTTGCGGCGCGGGGCa          >  2:386174/1‑151 (MQ=255)
                                                                                                                                         cAGCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGCa          <  2:8965494/151‑1 (MQ=255)
                                                                                                                                           gCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGCACg        >  2:345371/1‑151 (MQ=255)
                                                                                                                                           gCCGCGTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGCACg        >  2:10791308/1‑151 (MQ=255)
                                                                                                                                             cgcgTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGCACGTc      <  1:8954569/151‑1 (MQ=255)
                                                                                                                                              gcgTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGCACGTCg     <  1:3877171/151‑1 (MQ=255)
                                                                                                                                              gcgTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGCACGTCg     <  1:7645516/151‑1 (MQ=255)
                                                                                                                                               cgTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGCACGTCgg    >  1:5891035/1‑151 (MQ=255)
                                                                                                                                                gTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGCACGTCGgc   >  2:7099338/1‑151 (MQ=255)
                                                                                                                                                gTCCGGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAGGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGCACGTCGgc   <  2:5418719/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CGCCGAAATCGCCGGTGTGCAGGGGACGCATGGATTCGGTGACGAACTGCAGGGGGGTACGGTCGCTGAGCAAGCGGGTGGTTTCGATCTTGCCGGTATAAGGGTTCAGCGCGGCCGACTGGAACATGAGCGGATACCAGCCGCGTCCAGCCACGAACATCGGGCTGTAGGCGTTGCCGGGCAAGAACACCGCGCTGGCCTCCAGGCTGGGGATACGCCCGGTGACGAGCCGCACCGCCTCGTCCAGCTCGATGTATGGAACCTTCTGACCGGGTGCGGCGCGGGGCACGTCGGCG  >  NC_008463/2955285‑2955580

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑