Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 3,423,281 A→G K130K (AAA→AAG)  mexY → multidrug efflux RND transporter permease subunit MexY

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084633,423,2810AG98.3% 1507.1 / NA 469K130K (AAA→AAG) mexYmultidrug efflux RND transporter permease subunit MexY
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (254/207);  minor base T (8/0);  total (262/207)
Fisher's exact test for biased strand distribution p-value = 1.04e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 474 total aligned reads displayed.

CCAGCAGCACCGGCCAGGCTTCGCTGACCCTGACCTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAAGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTGGGCAAGGTCCAGACCTGGGGCGCC  >  NC_008463/3423133‑3423428
                                                                                                                                                    |                                                                                                                                                   
 cagcagCACCGGCCAGGCTTCGCTGACCCTGACCTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGCGGCGGTGCGGCGCGACGGTATCTACGGGGAGAAGgcg                                                                                                                                                  >  2:6077087/1‑151 (MQ=255)
   gcagcaCCGGCCAGGCTTCGCTGACCCTGACCTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAggcggc                                                                                                                                                >  2:4519107/1‑151 (MQ=255)
         acGGCCAGGCTTCGCTGACCCTGACCTACCGCAAGCGCGAGAACGCCGACCTCGCAGAGGTGGAAGTGCAGAACCGCATGAACATCGTCGAGTCGCGCCTGCCCGAGTCTGTGCGGCGCTCCGGTATCTAAGAGGAGAAGGCGGCGGCCAg                                                                                                                                          >  2:8392443/2‑151 (MQ=255)
          cGGCCAGGCTTCGCTGACCCTGACCTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTAAGTGGAGAAGGCGGCGGACAGc                                                                                                                                         >  2:2590801/1‑151 (MQ=255)
           ggCCAGGCTTCGCTGACCCTGACCTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCa                                                                                                                                        >  2:2356624/1‑151 (MQ=255)
             ccAGGCTTCGCTGACCCTGACCTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACCGCATc                                                                                                                                      >  2:7008226/1‑151 (MQ=255)
              cAGGCTTCGCTGACCCTGACCTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGGGGAGAAGGCGGCGGACAGCAAcc                                                                                                                                     >  1:6876686/1‑151 (MQ=255)
                ggCTTCGCTGACCCTGACCTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATATACGTGGAGAAGGCGGCGGACAGCCTCCAg                                                                                                                                   >  1:324316/1‑151 (MQ=255)
                   ttCGCTGACCCTGACCTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGACGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCAGCCCGAGTCGGGGCGGCGCGACGGTAGCTACGTGGAGAAGGCGGCGGACCGCATCCCGcgg                                                                                                                                >  2:3933113/1‑149 (MQ=255)
                   ttCGCTGACCCTGACCTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTg                                                                                                                                >  1:8675973/1‑151 (MQ=255)
                   ttCGCTGACCCTGACCTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTg                                                                                                                                >  2:7355122/1‑151 (MQ=255)
                     cGCTGACCCTGACCTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGGCGGTATCTACGGGGTGTAGGCGGCGGACAGCATCCCGCTggt                                                                                                                              >  1:4902430/1‑149 (MQ=255)
                       ctgaccctgaccTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCg                                                                                                                            <  2:4134211/151‑1 (MQ=255)
                        tgaccctgaccTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCgg                                                                                                                           >  2:973965/1‑150 (MQ=255)
                        tgaccctgaccTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGt                                                                                                                           >  2:1725000/1‑151 (MQ=255)
                          accctgaccTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCa                                                                                                                         >  2:6663364/1‑151 (MQ=255)
                           ccctgaccTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCAc                                                                                                                        <  2:420503/151‑1 (MQ=255)
                             ctgaccTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCAGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCAACCAGCAGATAGTCAccc                                                                                                                      >  1:2061859/1‑151 (MQ=255)
                              tgaccTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCt                                                                                                                     <  1:8123541/151‑1 (MQ=255)
                                accTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCa                                                                                                                   <  2:4747154/151‑1 (MQ=255)
                                 ccTTCCGCCAGGGCGGGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCAc                                                                                                                  <  2:3067443/151‑1 (MQ=255)
                                  cTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCAcc                                                                                                                 <  1:440373/151‑1 (MQ=255)
                                  cTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCAcc                                                                                                                 <  2:11412929/151‑1 (MQ=255)
                                           cggCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGc                                                                                                        <  2:87031/150‑1 (MQ=255)
                                            ggCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCg                                                                                                       <  1:10391984/151‑1 (MQ=255)
                                            ggCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCg                                                                                                       <  1:7224145/151‑1 (MQ=255)
                                            ggCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGGCGGTGCGGGGCGACGGGATCTACGGGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCCCCCTCACCGCCACCcgcg                                                                                                       >  1:1505794/1‑151 (MQ=255)
                                               gTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGcc                                                                                                    <  1:7740959/151‑1 (MQ=255)
                                               gTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGcc                                                                                                    <  2:5686067/151‑1 (MQ=255)
                                                 gAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGc                                                                                                  >  2:5499771/1‑151 (MQ=255)
                                                  aaCGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTAAGTGGAGAAGGCGGCGGACAGCATCCACCAGATCGTCACCCGCACCACCTCCAGCGGCCGCt                                                                                                 >  2:1980736/1‑151 (MQ=255)
                                                   aCGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTa                                                                                                >  1:10105869/1‑151 (MQ=255)
                                                    cGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTac                                                                                               <  2:8087552/151‑1 (MQ=255)
                                                     gCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTacg                                                                                              <  2:223590/151‑1 (MQ=255)
                                                     gCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTacg                                                                                              <  2:6561140/151‑1 (MQ=255)
                                                      ccGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTacga                                                                                             <  2:3765307/151‑1 (MQ=255)
                                                      ccGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTAAGTGGAGAAGGCGGCGGACAGCAAACAGCAGATCGTCACCCACACCACCTCCCGCGGCCGCTacga                                                                                             >  1:3685881/1‑151 (MQ=255)
                                                       cGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTacgac                                                                                            <  1:1845642/151‑1 (MQ=255)
                                                        gtccTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGGCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATATAAGGGGAGTATGCGGCGGACCGCATCCAGCTGATCGTCACCCTCACCGCCACCAGCGGCCGCTacgacg                                                                                           >  1:9390840/3‑151 (MQ=255)
                                                         aCCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCCCGCCTGCCCGACTCGGTGCGGCGCGACGGTATCTACGTGGTGAAGGCGGCGGACCGCAACCAGCTGATCGGCCCCCACACCGCCTCCACCGGCCGCTACCACGc                                                                                          >  1:461008/1‑151 (MQ=255)
                                                          ccTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCACCAGCGGCCGCTACGACGcc                                                                                         >  2:1492030/1‑151 (MQ=255)
                                                            tCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCAt                                                                                       <  2:3463620/151‑1 (MQ=255)
                                                            tCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGGGCGGCGCGACGGTATCTACGGGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCCGCGGCCGCTACGACGCCAt                                                                                       >  2:9045998/1‑151 (MQ=255)
                                                              gccgcGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATgg                                                                                     <  1:7521744/151‑1 (MQ=255)
                                                                cgcgGAGGAAGAGCAGACCCGCCTGAAGATCGTCGAGTCGCGCCGGCCCGAGTCGGTGCGGCTCGACGGTATCTACGTGGAGTAGGCGGCGGACAGCATCCAGCTGATCGTCTCCCTCTCCTCCTCCAGCGGCCGCTACGACGCCATGGaa                                                                                   <  2:5001304/151‑1 (MQ=255)
                                                                   ggTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTg                                                                                <  2:7150815/151‑1 (MQ=255)
                                                                   ggTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCAACGGGGAGGTGGCGGCGGGCAGCATACAGCTGGTCGTCAGCCTCACCACCTCCCGCGGCCGCTACGCCGCCATGGaccgg                                                                                >  2:10643119/1‑147 (MQ=255)
                                                                   ggTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCAGCCCCAGTCGGTGCGGCGCGGCGGGATCTACGTGGAGAAGGCGGCGGTCAGCATCCAGCTGATCGTCCCCCTCACCGCCTCCAGCGGCCGCGACGACGCCATTGAACTg                                                                                >  1:10012241/1‑151 (MQ=255)
                                                                    gTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATAGTCACCCACACCGCCACCAGCGGCCGCTACCACGCCATGGAACAgg                                                                               >  1:4263025/1‑151 (MQ=255)
                                                                     tGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTggg                                                                              <  1:3926608/151‑1 (MQ=255)
                                                                     tGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCGGCCCGAGTCGGTGCGGCGCGACGGTATCTACGGGGAGAAGGCGGCGGACAGCAACCAGCGGATCGTCACCCGCACCACCTCCCCCGGCCGCTACCACGCCCAGGGACTggg                                                                              >  1:3439304/1‑151 (MQ=255)
                                                                      ggAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTTTCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGGCACCCTCAACTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGc                                                                             >  2:10085781/1‑151 (MQ=255)
                                                                      ggAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGGATCTACGTGGTGAAGGCGGCGGACCGCATCCAGCTGATAGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGc                                                                             >  1:5330171/1‑151 (MQ=255)
                                                                      ggAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCGGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCCTCCACCTGATCGTCACCCTCACCTCCTCCCCCGGCCGCTACGACGCCAAGGAACTGGGc                                                                             >  1:10707346/1‑151 (MQ=255)
                                                                      ggAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCAGCCCGAGTCGGTGCGGCGCGACGGTATCTAAGTGGAGAAGGCGGCGGACAGCAACCAGCTGATCGTCACCCACACCACCACCCGCGGCCGCGACGACGCCAAGGAACTGGGc                                                                             >  1:2938662/1‑151 (MQ=255)
                                                                           tGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGTCGGTATCGACGTGGAGAAGGCGGCGGACAGCATCCAGCGGTTCGTCACCCTCACCTCCGCCAGCGGCCGCGACGACGCCAAGGAACTGGGCGAGAt                                                                        >  1:3778528/1‑151 (MQ=255)
                                                                           tGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGGTCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGAt                                                                        >  2:10323056/1‑151 (MQ=255)
                                                                             cAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTAACTACGTGGAGAAGGCGGCGGACAGCCTCCAGCTGATCGTCACCCTCACCACCTCCCGCGGCCGCTACGACGCCAAGGAACTGGGCGAGAACg                                                                      >  2:8777615/1‑151 (MQ=255)
                                                                              aGAACCGCCTGAAGATCGTCGAGTCGCGCCAGCCCGAGTCGGTGCGGCGCGACGGGATCTACGGGGAGAAGGCGGCGGACAGCATCCAGCGGATCGGCACCCACACCTCCTCCAGCGGCCGCTACGACGCCATGGAACAGGGCGAGATCGc                                                                     >  1:1589608/1‑151 (MQ=255)
                                                                                    gCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGAGAGTCACCCACACCTCCTCCAGCGGCCGCTACGACGCCAAGGAACAGGGCGCGATCGCCTCGTc                                                               >  2:7877877/1‑151 (MQ=255)
                                                                                    gCCTGAAGATCGTCGAGTCGCGCCTGCCCCAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCAGATCGTCACCCTCACCTCCACCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATAGCCTCGTc                                                               >  1:8239922/1‑151 (MQ=255)
                                                                                             tcgtcgAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGtt                                                      >  2:6376949/1‑151 (MQ=255)
                                                                                             tcgtcgAGTCGCGCCGGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGGCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGAGCGCCTCGTCCAACgtgtg                                                      >  2:4366171/1‑150 (MQ=255)
                                                                                              cgtcgAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTg                                                     <  2:8645055/151‑1 (MQ=255)
                                                                                              cgtcgAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTAAGTGGAGAAGGCCGCGGACAGCAACCAGCTGATCGTCACCCTCACCTCCACCAGCGGCCGCTACGACGCCAAGGAACAGGGCGAGATCGCCTCGTCCAACGTGATg                                                     >  1:9055544/1‑151 (MQ=255)
                                                                                               gtcgAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGc                                                    >  2:5974100/1‑151 (MQ=255)
                                                                                                tcgAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCa                                                   <  2:1276132/151‑1 (MQ=255)
                                                                                                 cgAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTTCGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAg                                                  <  1:9430640/151‑1 (MQ=255)
                                                                                                  gAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCCGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAgg                                                 >  1:2500296/1‑151 (MQ=255)
                                                                                                   aGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGgc                                                <  1:6373960/151‑1 (MQ=255)
                                                                                                       gcgcCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTg                                            <  1:7437432/151‑1 (MQ=255)
                                                                                                        cgcCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTgc                                           <  2:630420/151‑1 (MQ=255)
                                                                                                        cgcCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTgc                                           <  1:7925483/151‑1 (MQ=255)
                                                                                                        cgcCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTgc                                           >  1:1759397/1‑151 (MQ=255)
                                                                                                         gcCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTgcg                                          >  1:5462050/1‑151 (MQ=255)
                                                                                                           cTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGcc                                        <  2:9459253/151‑1 (MQ=255)
                                                                                                           cTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGcc                                        <  1:8460369/151‑1 (MQ=255)
                                                                                                             gCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCgg                                      >  1:9608365/1‑151 (MQ=255)
                                                                                                                 gAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTgg                                  >  2:7694525/1‑151 (MQ=255)
                                                                                                                 gAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTgg                                  <  2:9937174/151‑1 (MQ=255)
                                                                                                                  aGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGa                                 >  1:11363431/1‑151 (MQ=255)
                                                                                                                   gTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAg                                <  1:10947928/151‑1 (MQ=255)
                                                                                                                   gTCGGTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAg                                >  1:6079032/1‑151 (MQ=255)
                                                                                                                      ggTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGc                             <  2:10996429/151‑1 (MQ=255)
                                                                                                                      ggTGCGGCGCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGc                             <  1:5923675/151‑1 (MQ=255)
                                                                                                                         gcggcgCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTg                          <  2:3631568/151‑1 (MQ=255)
                                                                                                                          cggcgCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTgg                         <  1:7867332/151‑1 (MQ=255)
                                                                                                                           ggcgCGACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTggg                        <  2:9185357/151‑1 (MQ=255)
                                                                                                                            gcgcGACGGTCTCTACGTGGAGAAGGCGGCGGACAGCATCCAGATGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTGGGc                       <  2:691520/151‑1 (MQ=255)
                                                                                                                             cgcgACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTGGGCa                      >  2:2801346/1‑151 (MQ=255)
                                                                                                                              gcgACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCATCGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTGGGCaa                     >  2:7514265/1‑151 (MQ=255)
                                                                                                                               cgACGGTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTGGGCAAg                    <  1:8742111/151‑1 (MQ=255)
                                                                                                                                    gTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTGGGCAAGGTcga               >  1:665173/1‑149 (MQ=255)
                                                                                                                                    gTATCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTGGGCAAGGTcga               >  1:2311562/1‑149 (MQ=255)
                                                                                                                                       tCTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTGGGCAAGGTCGAGAc            >  1:4148814/1‑151 (MQ=255)
                                                                                                                                        cTACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTGGGCAAGGTCGAGAcc           >  1:7036731/1‑151 (MQ=255)
                                                                                                                                         tACGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTGGGCAAGGTCGAGACCt          >  1:3999741/1‑151 (MQ=255)
                                                                                                                                           cGTGGAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTGGGCAAGGTCGAGACCTgg        >  1:1297893/1‑151 (MQ=255)
                                                                                                                                              ggAGAAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTGGGCAAGGTCGAGACCTGGGgc     <  2:8997565/151‑1 (MQ=255)
                                                                                                                                                agaAGGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTGGGCAAGGTCGAGACCTGGGgcgc   >  2:542685/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CCAGCAGCACCGGCCAGGCTTCGCTGACCCTGACCTTCCGCCAGGGCGTGAACGCCGACCTCGCCGCGGTGGAAGTGCAGAACCGCCTGAAGATCGTCGAGTCGCGCCTGCCCGAGTCGGTGCGGCGCGACGGTATCTACGTGGAGAAAGCGGCGGACAGCATCCAGCTGATCGTCACCCTCACCTCCTCCAGCGGCCGCTACGACGCCATGGAACTGGGCGAGATCGCCTCGTCCAACGTGTTGCAGGCGCTGCGCCGGGTGGAGGGCGTGGGCAAGGTCCAGACCTGGGGCGCC  >  NC_008463/3423133‑3423428

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑