Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 3,670,384 A→G Q128Q (CAA→CAG)  PA14_RS16675 → ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084633,670,3840AG99.5% 1614.8 / ‑2.0 417Q128Q (CAA→CAG) PA14_RS16675ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (219/196);  minor base C (2/0);  total (221/196)
Fisher's exact test for biased strand distribution p-value = 5.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

Only 100 of 417 total aligned reads displayed.

GCCCCTACATCAGCCAACTGATCGCCGAGAAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAAGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAG  >  NC_008463/3670236‑3670531
                                                                                                                                                    |                                                                                                                                                   
  cccTACATCAGCCAACTGATCGCCGAGAAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGccc                                                                                                                                                 <  1:6391651/151‑1 (MQ=255)
  cccTACATCAGCCAACTGATCGCCGAGAAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGccc                                                                                                                                                 <  2:1278488/151‑1 (MQ=255)
  cccTACATCAGCCAACTGATCGCCGAGAAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGccc                                                                                                                                                 >  1:9569870/1‑151 (MQ=255)
    cTACATCAGCCAACTGATCGCCGAGAAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCcgc                                                                                                                                               <  1:11332322/151‑1 (MQ=255)
    cTACATCAGCCAACTGATCGCCGAGAAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACAACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGACcgc                                                                                                                                               >  2:3732534/1‑151 (MQ=255)
       cctCAGCCAACTGATCGCCGAGAAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGAc                                                                                                                                            <  1:1870690/149‑1 (MQ=255)
       cATCAGCCAACTGATCGCCGAGAAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGAc                                                                                                                                            <  1:8830916/151‑1 (MQ=255)
        aTCAGCCAACTGATCGCCGAGAAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGACTACTGGCTGGGCACCGACGACCAGGCCCGCGACg                                                                                                                                           >  2:8515269/1‑151 (MQ=255)
        aTCAGCCAACTGATCGCCGAGAAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACg                                                                                                                                           >  1:2615319/1‑151 (MQ=255)
          cAGCCAACTGATCGCCGAGAAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTg                                                                                                                                         >  1:11102987/1‑151 (MQ=255)
            gCCAACTGATCGCCGAGAAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCt                                                                                                                                       >  2:10916776/1‑151 (MQ=255)
                  tGATCGCCGAGAAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGTCACCCTCATCTACGAGCTGTAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAAGTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCg                                                                                                                                 >  1:284589/1‑151 (MQ=38)
                  tGATCGCCGAGAAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCg                                                                                                                                 >  1:695001/1‑151 (MQ=255)
                    aTCGCCGAGAAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCggg                                                                                                                               >  1:7703942/1‑151 (MQ=255)
                      cGCCGAGAAGGACGGCTGGGTGCTTTTGCCGCCGATCCCCTACAGCTACGACACCATAAACTACGAGCAGAAGGTTCACGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGAACCGACGACCAGGCCCGCGACGTGCTCGCACGGGTg                                                                                                                             >  2:10794169/1‑151 (MQ=255)
                      cGCCGAGAAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTCCAGCTACGACTCCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTg                                                                                                                             <  2:7616702/151‑1 (MQ=255)
                      cGCCGAGAAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTg                                                                                                                             >  2:7820362/1‑151 (MQ=255)
                      cGCCGAGAAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTg                                                                                                                             <  2:9248450/151‑1 (MQ=255)
                      cGCCGAGAAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTg                                                                                                                             <  1:6716261/151‑1 (MQ=255)
                       gCCGAGAAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGa                                                                                                                            <  1:5408121/151‑1 (MQ=255)
                       gCCGAGAAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGa                                                                                                                            <  1:10937882/151‑1 (MQ=255)
                          gagaAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGAttt                                                                                                                         >  2:2212007/1‑151 (MQ=255)
                            gaAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTAc                                                                                                                       >  2:7300138/1‑151 (MQ=255)
                            gaAGGACGGCTGGGAGCTTTGGCCGACCATCCCATACAGCTAAGACACCATCAACTACGAGATGAAGGTTCACGCCCCAGCCCCGCCTTCGGCGGAAAACTGGCTGGGCACAGACGACCAGGCCCGCGACGAGCTCGCCCGGGTGATTTAc                                                                                                                       >  2:10616191/1‑151 (MQ=255)
                             aaGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACg                                                                                                                      <  2:5777337/151‑1 (MQ=255)
                              aGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACgg                                                                                                                     <  2:8143715/151‑1 (MQ=255)
                                 aCGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCtt                                                                                                                  <  2:6078487/151‑1 (MQ=255)
                                     cTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCcgc                                                                                                              <  2:4903318/151‑1 (MQ=255)
                                     cTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCcgc                                                                                                              >  1:2265007/1‑151 (MQ=255)
                                     cTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCcgc                                                                                                              <  2:9566022/151‑1 (MQ=255)
                                       gggTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCACCTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCgt                                                                                                            <  1:4282601/151‑1 (MQ=255)
                                         gTGCTTTGGCCGCCGATCCCCTCCAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCgtgt                                                                                                          <  2:2474243/151‑1 (MQ=255)
                                         gTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCgtgt                                                                                                          <  1:3429534/151‑1 (MQ=255)
                                         gTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCgtgt                                                                                                          <  1:2032973/151‑1 (MQ=255)
                                          tGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTc                                                                                                         <  1:3286926/151‑1 (MQ=255)
                                          tGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTc                                                                                                         >  1:9779455/1‑151 (MQ=255)
                                           gCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCg                                                                                                        <  2:4657257/151‑1 (MQ=255)
                                           gCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCg                                                                                                        >  2:9823802/1‑151 (MQ=255)
                                                  ccgccgATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCctct                                                                                                 <  2:11070681/151‑1 (MQ=255)
                                                   cgccgATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCtt                                                                                                >  2:2059814/1‑151 (MQ=255)
                                                     ccgATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCg                                                                                              <  1:9357849/151‑1 (MQ=255)
                                                      cgATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGc                                                                                             >  2:5527343/1‑151 (MQ=255)
                                                       gATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGcc                                                                                            >  1:10161610/1‑151 (MQ=255)
                                                         tCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGccct                                                                                          >  2:11297854/1‑151 (MQ=255)
                                                            ccTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGccctgac                                                                                       >  1:2778289/1‑151 (MQ=255)
                                                              tACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGccctgaccc                                                                                     <  1:7868280/151‑1 (MQ=255)
                                                              tACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGccctgaccc                                                                                     >  2:4176653/1‑151 (MQ=255)
                                                               aCAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGccctgaccct                                                                                    <  2:275229/151‑1 (MQ=255)
                                                               aCAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGccctgaccct                                                                                    >  2:7050111/1‑151 (MQ=255)
                                                                cAGCTACGACACCATCAACTACGAGCTGAAGGGTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGccctgaccctg                                                                                   <  1:3858636/151‑1 (MQ=255)
                                                                 aGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGccctgaccctga                                                                                  >  2:2547524/1‑151 (MQ=255)
                                                                  gCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCcctgaccctgac                                                                                 >  1:7471017/1‑151 (MQ=255)
                                                                    tACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGACAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCg                                                                               >  2:674085/1‑151 (MQ=255)
                                                                    tACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCg                                                                               <  2:4369863/151‑1 (MQ=255)
                                                                     aCGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGt                                                                              <  1:4060250/151‑1 (MQ=255)
                                                                     aCGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGt                                                                              <  1:8716571/151‑1 (MQ=255)
                                                                      cGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTg                                                                             <  1:5943269/151‑1 (MQ=255)
                                                                        acacCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCt                                                                           >  1:5178920/1‑151 (MQ=255)
                                                                        acacCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCt                                                                           <  2:6268611/151‑1 (MQ=255)
                                                                        acacCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCt                                                                           >  2:6192174/1‑151 (MQ=255)
                                                                         cacCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTc                                                                          >  2:10427567/1‑151 (MQ=255)
                                                                          acCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCa                                                                         >  2:3530890/1‑151 (MQ=255)
                                                                           ccATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAg                                                                        <  2:8171660/151‑1 (MQ=255)
                                                                           ccATCAACTACGAGCTGAAGGGTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAg                                                                        <  2:6941126/151‑1 (MQ=255)
                                                                             aTCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACAGGCTGGGCACCGACGACCAGGCCCGCGACGAGCACGCCCGGGTGATTTACGGCTTCCGCGTGAAGAACCTCATCGACCTGACCCTGAACGTGATCAggt                                                                      >  2:4578599/1‑149 (MQ=25)
                                                                               cAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTtct                                                                    >  2:2875606/1‑151 (MQ=255)
                                                                               cAACGACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTtct                                                                    <  2:6496696/151‑1 (MQ=255)
                                                                                aaCTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTtctc                                                                   <  2:1653759/151‑1 (MQ=255)
                                                                                 aCTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGTCCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTctct                                                                  <  2:1538060/151‑1 (MQ=255)
                                                                                  cTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTg                                                                 >  1:8498991/1‑151 (MQ=255)
                                                                                  cTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTg                                                                 >  1:4807723/1‑151 (MQ=255)
                                                                                   tACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGa                                                                <  1:7121202/151‑1 (MQ=255)
                                                                                    aCGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGAt                                                               <  1:7995841/151‑1 (MQ=255)
                                                                                      gAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCg                                                             >  2:3661618/1‑151 (MQ=255)
                                                                                         cTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCg                                                          >  2:10254787/1‑151 (MQ=255)
                                                                                          tGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGt                                                         >  1:6300250/1‑151 (MQ=255)
                                                                                          tGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGt                                                         >  1:901956/1‑151 (MQ=255)
                                                                                             aGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCAc                                                      <  1:10409249/151‑1 (MQ=255)
                                                                                              ggTTCCCGCCCCCGCCCCGCCGTCGGCGGCAAACTGGCTGGGCACCGACGAACAGCCCCGCGACGTGCTCGCCCGGGTGATTAACGGCTTCAGCGTGTCGATCCTCTTCTCCCTGACCCTGACCGTGCTCAGTTCTCAGATCGGCGTCAcc                                                     >  2:3329152/1‑151 (MQ=255)
                                                                                                ttCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGt                                                   >  1:7261763/1‑151 (MQ=255)
                                                                                                 tCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTc                                                  >  2:9451048/1‑151 (MQ=255)
                                                                                                  cccgcccccgccCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCg                                                 <  1:10037427/151‑1 (MQ=255)
                                                                                                     gcccccgccccGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGgcg                                              >  1:5305489/1‑151 (MQ=255)
                                                                                                      cccccgccccGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGgcgc                                             >  1:6526485/1‑151 (MQ=255)
                                                                                                      cccccgccccGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGACCCTCATCGCGCTGACCCTGTCAGTGCTCAGTTATCTGATCGGAGTCACAGTCGgcgc                                             >  2:4066517/1‑151 (MQ=25)
                                                                                                           gccccgccGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGc                                        >  1:9558062/1‑151 (MQ=255)
                                                                                                           gccccgccGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGc                                        >  1:1416645/1‑151 (MQ=255)
                                                                                                           gccccgccGTCGGCGGAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGc                                        >  2:2367559/1‑151 (MQ=255)
                                                                                                                     cggcggAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGctacta                              >  1:1606439/1‑151 (MQ=255)
                                                                                                                      ggcggAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCtactac                             >  1:6112877/1‑151 (MQ=255)
                                                                                                                        cggAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTAcgg                           >  2:3151299/1‑151 (MQ=255)
                                                                                                                         ggAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTAcggc                          <  2:8914850/151‑1 (MQ=255)
                                                                                                                          gAAAACTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTAcggcg                         >  1:10602406/1‑151 (MQ=255)
                                                                                                                             aaCTGGCTGGGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCt                      <  2:6321415/151‑1 (MQ=255)
                                                                                                                               ctggctggGCACCGACGACCACGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTgg                    >  1:8161054/1‑151 (MQ=255)
                                                                                                                                  gctggGCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTc                 <  2:7559501/151‑1 (MQ=255)
                                                                                                                                       gCACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCt            >  1:10989891/1‑151 (MQ=255)
                                                                                                                                        cACCGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTg           <  1:4651147/151‑1 (MQ=255)
                                                                                                                                          ccGACGACCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCt         <  2:500756/151‑1 (MQ=255)
                                                                                                                                            gacgacCAGGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCg       >  2:3409600/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GCCCCTACATCAGCCAACTGATCGCCGAGAAGGACGGCTGGGTGCTTTGGCCGCCGATCCCCTACAGCTACGACACCATCAACTACGAGCTGAAGGTTCCCGCCCCCGCCCCGCCGTCGGCGGAAAACTGGCTGGGCACCGACGACCAAGCCCGCGACGTGCTCGCCCGGGTGATTTACGGCTTCCGCGTGTCGATCCTCTTCGCCCTGACCCTGACCGTGCTCAGTTCTCTGATCGGCGTCACCGTCGGCGCCCTGCAAGGCTACTACGGCGGCTGGGTCGACCTGCTCGGCCAG  >  NC_008463/3670236‑3670531

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑