Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 4,707,787 G→C A208A (GCG→GCC)  PA14_RS21625 → zinc‑binding alcohol dehydrogenase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084634,707,7870GC100.0% 2450.1 / NA 624A208A (GCG→GCC) PA14_RS21625zinc‑binding alcohol dehydrogenase family protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (278/346);  total (278/346)

Only 100 of 657 total aligned reads displayed.

TGGCCAAGGCCCTGGGGGCGGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCGGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGAACCTGTACCGTCGCGGTGGCTCGGTG  >  NC_008463/4707639‑4707934
                                                                                                                                                    |                                                                                                                                                   
 ggcCAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAg                                                                                                                                                  >  2:867752/1‑151 (MQ=255)
    cAAGGCCCTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTg                                                                                                                                               <  2:4302006/151‑1 (MQ=255)
         cccTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCtt                                                                                                                                          <  1:3026036/151‑1 (MQ=255)
          ccTGGGGGCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTc                                                                                                                                         <  1:6342054/151‑1 (MQ=255)
               gggCCGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATAc                                                                                                                                    <  2:8568022/151‑1 (MQ=255)
                  ccgAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGAc                                                                                                                                 >  2:2372556/3‑151 (MQ=255)
                    gAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCg                                                                                                                               <  1:3106390/151‑1 (MQ=255)
                     aaGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCgg                                                                                                                              >  2:9796917/1‑151 (MQ=255)
                     aaGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCgg                                                                                                                              >  2:11458383/1‑151 (MQ=255)
                     aaGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCgg                                                                                                                              >  1:7466700/1‑151 (MQ=255)
                     aaGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCgc                                                                                                                              <  1:7263815/151‑2 (MQ=255)
                      aGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCCCAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGt                                                                                                                             >  2:4652964/1‑151 (MQ=255)
                      aGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGt                                                                                                                             >  2:2610671/1‑151 (MQ=255)
                        cGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGttt                                                                                                                           <  2:7661069/151‑1 (MQ=255)
                        cGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGttt                                                                                                                           >  1:7149218/1‑151 (MQ=255)
                           tCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTg                                                                                                                        >  2:7879942/1‑151 (MQ=255)
                            cGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTgg                                                                                                                       <  2:108195/151‑1 (MQ=255)
                               cgcgGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTc                                                                                                                    >  2:614138/1‑151 (MQ=255)
                               cgcgGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTc                                                                                                                    >  2:7130263/1‑151 (MQ=255)
                                  ggTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAg                                                                                                                 <  2:3058709/151‑1 (MQ=255)
                                  ggTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAg                                                                                                                 <  1:7050360/151‑1 (MQ=255)
                                   gTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAgg                                                                                                                >  1:2622/1‑151 (MQ=255)
                                    tGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAggc                                                                                                               <  1:2136297/151‑1 (MQ=255)
                                     gcggcgCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAggcg                                                                                                              >  1:9509715/1‑151 (MQ=255)
                                      cggcgCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAggcgg                                                                                                             >  1:1509307/1‑151 (MQ=255)
                                          gcgAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGt                                                                                                         >  2:8062555/1‑151 (MQ=255)
                                            gaggagCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTcc                                                                                                       <  1:11031341/151‑1 (MQ=255)
                                            gaggagCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTcc                                                                                                       >  2:8762691/1‑151 (MQ=255)
                                            gaggagCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTcc                                                                                                       >  2:7242634/1‑151 (MQ=255)
                                            gaggagCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGGTTCTGGCTCGAGGCGGCGGTcc                                                                                                       >  2:4029280/1‑151 (MQ=255)
                                             aggagCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTccc                                                                                                      <  1:5571725/151‑1 (MQ=255)
                                              ggagCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCg                                                                                                     >  2:2282848/1‑151 (MQ=255)
                                               gagCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCgg                                                                                                    >  1:5148128/1‑151 (MQ=255)
                                               gagCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCgg                                                                                                    >  1:2224746/1‑151 (MQ=255)
                                                agCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGgc                                                                                                   <  1:8059063/151‑1 (MQ=255)
                                                  cAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGgcgc                                                                                                 >  2:3248948/1‑151 (MQ=255)
                                                  cAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGgcgc                                                                                                 >  2:5502527/1‑151 (MQ=255)
                                                  cAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGgcgc                                                                                                 >  2:6655001/1‑151 (MQ=255)
                                                   aaCGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCt                                                                                                >  2:9278151/1‑151 (MQ=255)
                                                   aaCGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCt                                                                                                <  1:10319103/151‑1 (MQ=255)
                                                   aaCGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCt                                                                                                >  1:10853195/1‑151 (MQ=255)
                                                    aCGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTg                                                                                               >  1:9210241/1‑151 (MQ=255)
                                                      gCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGgc                                                                                             >  1:11230424/1‑151 (MQ=255)
                                                       ccAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGgcc                                                                                            <  1:3361056/151‑1 (MQ=255)
                                                          ggCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTTGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGgccgcc                                                                                         >  1:4548844/1‑151 (MQ=255)
                                                           gcgcTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCt                                                                                        <  2:9607586/151‑1 (MQ=255)
                                                           gcgcTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCt                                                                                        <  2:8419315/151‑1 (MQ=255)
                                                            cgcTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCtt                                                                                       >  1:9047679/1‑151 (MQ=255)
                                                             gcTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTc                                                                                      <  2:10426585/151‑1 (MQ=255)
                                                              cTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCg                                                                                     <  2:2905564/151‑1 (MQ=255)
                                                               tGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCgg                                                                                    >  1:7599665/1‑151 (MQ=255)
                                                                ggAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGt                                                                                   <  1:60509/151‑1 (MQ=255)
                                                                ggAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGt                                                                                   >  1:10610554/1‑151 (MQ=255)
                                                                     cgcAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCt                                                                              <  2:5145217/151‑1 (MQ=255)
                                                                     cgcAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCt                                                                              <  1:8714782/151‑1 (MQ=255)
                                                                      gcAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTg                                                                             <  1:6666672/151‑1 (MQ=255)
                                                                             aTCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGatca                                                                      >  1:3192210/1‑151 (MQ=255)
                                                                              tCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGatcat                                                                     <  2:1535419/151‑1 (MQ=255)
                                                                               cgccgcGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGatcatc                                                                    <  2:8278715/151‑1 (MQ=255)
                                                                                  cgcgCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATcgcc                                                                 <  2:1737346/151‑1 (MQ=255)
                                                                                   gcgcTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACCGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATcgccg                                                                <  1:6160843/151‑1 (MQ=255)
                                                                                    cgcTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATcgccgc                                                               <  2:5259919/151‑1 (MQ=255)
                                                                                        gctgctgGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCGTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCa                                                           <  1:232419/151‑1 (MQ=255)
                                                                                              gggCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGAc                                                     <  2:9068388/151‑1 (MQ=255)
                                                                                               ggCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACg                                                    <  1:10027069/151‑1 (MQ=255)
                                                                                                      cggccgAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCAtgt                                             >  2:6840721/1‑151 (MQ=255)
                                                                                                      cggccgAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCAtgt                                             >  2:4952777/1‑151 (MQ=255)
                                                                                                       ggccgAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCAtgtg                                            <  1:1352794/151‑1 (MQ=255)
                                                                                                          cgAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTgcgc                                         >  2:10003038/1‑151 (MQ=255)
                                                                                                           gAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGcc                                        <  1:2841296/151‑1 (MQ=255)
                                                                                                            caCTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCt                                       <  2:7736178/150‑1 (MQ=255)
                                                                                                            accTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCt                                       <  2:6969219/149‑1 (MQ=255)
                                                                                                            aaCTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCt                                       >  2:11057766/1‑151 (MQ=255)
                                                                                                               tGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCGTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGcc                                    >  2:5896601/1‑151 (MQ=255)
                                                                                                                     cacaGGTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGtt                              >  2:11265286/1‑151 (MQ=255)
                                                                                                                         ggTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGAAc                          >  2:1905757/1‑151 (MQ=255)
                                                                                                                         ggTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGAAc                          >  1:3591886/1‑151 (MQ=255)
                                                                                                                         ggTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGAAc                          >  1:4270169/1‑151 (MQ=255)
                                                                                                                          gTGGAAGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGAAcc                         >  1:5331193/1‑151 (MQ=255)
                                                                                                                               aGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGAACCTGTAc                    <  2:47991/151‑1 (MQ=255)
                                                                                                                               aGGGATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGAACCTGTAc                    <  1:4857881/151‑1 (MQ=255)
                                                                                                                                gggATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGAACCTGTAcc                   >  1:6785484/1‑151 (MQ=255)
                                                                                                                                gggATCGTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGAACCTGTAcc                   <  2:3577789/151‑1 (MQ=255)
                                                                                                                                 ggATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGAACCTGTACCg                  >  1:8475912/1‑151 (MQ=255)
                                                                                                                                  gATCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGAACCTGTACCGt                 <  2:5193333/151‑1 (MQ=255)
                                                                                                                                  gATCTGCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGAACCTGTACCGt                 <  1:3798617/151‑1 (MQ=255)
                                                                                                                                   aTCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGAACCTGTACCGTc                <  1:11371224/151‑1 (MQ=255)
                                                                                                                                    tCTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGACCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGAACCTGTACCGTcg               >  1:1896658/1‑151 (MQ=255)
                                                                                                                                     cTTCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGAACCTGTACCGTcgc              <  2:6329001/151‑1 (MQ=255)
                                                                                                                                      ttCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGAACCTGTACCGTcgcg             <  1:9784533/151‑1 (MQ=255)
                                                                                                                                      ttCCCCGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGAACCTGTACCGTcgcg             >  1:8332879/1‑151 (MQ=255)
                                                                                                                                        ccccGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGTGGCGGCGGTCCCGGCGCTGGCCGCCTGCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCCTGTGCGCCTGCCGGCGTTGAACCTGTACCGTCGCGGt           >  2:1368299/1‑151 (MQ=255)
                                                                                                                                        ccccGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGAACCTGTACCGTCGCGGt           >  2:3829933/1‑151 (MQ=255)
                                                                                                                                         cccGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGAACCTGTACCGTCGCGGTg          <  2:5715761/151‑1 (MQ=255)
                                                                                                                                         cccGGCGGGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGAACCTGTACCGTCGCGGTg          <  2:2155844/151‑1 (MQ=255)
                                                                                                                                           cggcggGGCCGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGAACCTGTACCGTCGCGGTGGc        <  1:2541615/151‑1 (MQ=255)
                                                                                                                                            ggcggggccgAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGAACCTGTACCGTCGCGGTGGCt       <  2:9470378/151‑1 (MQ=255)
                                                                                                                                               ggggccgAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGAACCTGTACCGTCGCGGTGGCTCgg    >  1:5067946/1‑151 (MQ=255)
                                                                                                                                                gggccgAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGAACCTGTACCGTCGCGGTGGCTCggt   <  1:7913184/151‑1 (MQ=255)
                                                                                                                                                gggccgAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGAACCTGTACCGTCGCGGTGGCTCggt   <  1:9380943/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TGGCCAAGGCCCTGGGGGCGGAAGCGATCGGCGCGGTGCGGCGCGAGGAGCAACGCCAGGCGCTGGAAGCGCAGGGCATCGCCGCGCTGCTGCTGGGCGAGCCGGCCGAACTGGCGGCACAGGTGGAAGGGATCTTCCCCGGCGGGGCGGAGGTGATCTTCGATACGACCGGTTTCTGGCTCGAGGCGGCGGTCCCGGCGCTGGCCGCCTTCGGTCGCCTGGCGATCATCGCCGCACCAGCCGACGGCCATGTGCGCCTGCCGGCGTTGAACCTGTACCGTCGCGGTGGCTCGGTG  >  NC_008463/4707639‑4707934

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑