Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 480,915 T→C K193E (AAA→GAA)  PA14_RS02220 ← 16S rRNA (uracil(1498)‑N(3))‑methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_008463480,9150TC99.8% 2157.2 / NA 551K193E (AAA→GAA) PA14_RS0222016S rRNA (uracil(1498)‑N(3))‑methyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (294/256);  minor base G (1/0);  total (295/256)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 551 total aligned reads displayed.

TATCAGGCGAAGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTTGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTTGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGTCCTCCAGGGCCAGCGGCGGATGGATCAGCGGCAGGCTCGA  >  NC_008463/480767‑481062
                                                                                                                                                    |                                                                                                                                                   
tatCAGGCGAAGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCgg                                                                                                                                                   >  1:8512830/1‑151 (MQ=255)
tatCAGGCGAAGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCgg                                                                                                                                                   >  2:8669479/1‑151 (MQ=255)
tatCAGGCGAAGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCgg                                                                                                                                                   >  2:3779718/1‑151 (MQ=255)
 atCAGGCGAAGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCggg                                                                                                                                                  >  1:11126552/1‑151 (MQ=255)
  tCAGGCGAAGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGa                                                                                                                                                 >  1:10869129/1‑151 (MQ=255)
   cAGGCGAAGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGAc                                                                                                                                                >  1:9669244/1‑151 (MQ=255)
    aGGCGAAGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGAcc                                                                                                                                               <  1:3279257/151‑1 (MQ=255)
     ggCGAAGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGAccc                                                                                                                                              >  1:8115261/1‑151 (MQ=255)
      gCGAAGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACcca                                                                                                                                             <  2:1743349/151‑1 (MQ=255)
       cGAAGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACccac                                                                                                                                            >  2:8607468/1‑151 (MQ=255)
        gAAGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACccacc                                                                                                                                           <  2:898044/151‑1 (MQ=255)
         aaGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACccaccc                                                                                                                                          >  2:4171187/1‑150 (MQ=255)
              gCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAg                                                                                                                                     >  2:10897690/1‑151 (MQ=255)
              gCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAg                                                                                                                                     <  1:7285923/151‑1 (MQ=255)
               ccccAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAgg                                                                                                                                    <  2:4328049/151‑1 (MQ=255)
                cccAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGc                                                                                                                                   <  1:3175484/151‑1 (MQ=255)
                 ccAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGcc                                                                                                                                  >  1:753977/1‑151 (MQ=255)
                  cAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCa                                                                                                                                 >  2:6574500/1‑151 (MQ=255)
                   agagTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAg                                                                                                                                <  2:508758/151‑1 (MQ=255)
                   agagTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAg                                                                                                                                >  1:10261816/1‑151 (MQ=255)
                    gagTTGCTGGGCCCCCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAgg                                                                                                                               <  2:6093658/151‑1 (MQ=255)
                      gTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGgcg                                                                                                                             <  1:10697154/151‑1 (MQ=255)
                      gTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGgcg                                                                                                                             >  2:2533504/1‑151 (MQ=255)
                        tGCTGGGCCACCGCCAACGCTACCACCGGCGAGGTCTCGGTGCGCAGCTCTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGGCGCCTTCGGGACCCACCAGGAAGGCCAGGcgcg                                                                                                                           <  2:2757504/151‑1 (MQ=255)
                          cTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGAc                                                                                                                         >  2:6255725/1‑151 (MQ=255)
                              gCCACCGCCAACGCTACCACCGGTGAGGTCTCGGTGCGCAGGAGTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGAcggcg                                                                                                                     <  1:9947872/151‑1 (MQ=255)
                              gCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGAcggcg                                                                                                                     <  2:8185749/151‑1 (MQ=255)
                              gCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGAcggcg                                                                                                                     >  1:5584377/1‑151 (MQ=255)
                                  ccGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGcac                                                                                                                 <  2:8865745/151‑1 (MQ=255)
                                  ccGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGcac                                                                                                                 <  2:8392940/151‑1 (MQ=255)
                                    gCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACAt                                                                                                               <  1:10105179/151‑1 (MQ=255)
                                    gCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCGCGGCATCGCTCAGGCCGCCTTCGGGACCCTCCAGGAAGGCCAGGCGCGACGGCGGCACAt                                                                                                               >  1:10443386/1‑151 (MQ=255)
                                        aCGCGACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACAtggct                                                                                                           <  2:5047594/151‑1 (MQ=255)
                                          gCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCCCCAGGACGGCCAGGCGCGACGGCGGCTCAtggctgg                                                                                                         >  2:7725278/1‑151 (MQ=255)
                                            tACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGcc                                                                                                       <  2:120758/151‑1 (MQ=255)
                                            tACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGcc                                                                                                       <  2:5423871/151‑1 (MQ=255)
                                            tACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGcc                                                                                                       <  2:3086048/151‑1 (MQ=255)
                                             accaccGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCa                                                                                                      <  1:405658/151‑1 (MQ=255)
                                                accGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAggg                                                                                                   >  2:3360520/1‑151 (MQ=255)
                                                  cGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAggggg                                                                                                 <  2:11101159/151‑1 (MQ=255)
                                                   ggCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAgggggg                                                                                                >  2:9265863/1‑151 (MQ=255)
                                                      gcgGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGggcgg                                                                                             <  1:3551462/151‑1 (MQ=255)
                                                      gcgGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGggcgg                                                                                             <  2:2972009/151‑1 (MQ=255)
                                                                tGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTg                                                                                   <  1:8901574/151‑1 (MQ=255)
                                                                    cagcaCTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAgg                                                                               >  1:7895848/1‑151 (MQ=255)
                                                                      gcaCTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGAc                                                                             <  1:3780365/151‑1 (MQ=255)
                                                                        aCTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCa                                                                           <  1:2147033/151‑1 (MQ=255)
                                                                         cTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCAACGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAg                                                                          >  1:1416765/1‑151 (MQ=255)
                                                                              gggCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGa                                                                     >  2:2576224/1‑151 (MQ=255)
                                                                              gggCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGa                                                                     <  2:10283049/151‑1 (MQ=255)
                                                                              gggCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGa                                                                     <  1:6644154/151‑1 (MQ=255)
                                                                                  cGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTc                                                                 <  1:6168693/151‑1 (MQ=255)
                                                                                   gAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCa                                                                >  1:9313806/1‑151 (MQ=255)
                                                                                   gAGGCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCa                                                                >  1:11321529/1‑151 (MQ=255)
                                                                                     ggCGCGCGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATc                                                              >  2:9747961/1‑151 (MQ=255)
                                                                                      gcgcgcGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCg                                                             <  1:4235193/151‑1 (MQ=255)
                                                                                      gcgcgcGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCg                                                             >  2:4500312/1‑151 (MQ=255)
                                                                                      gcgcgcGGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCg                                                             >  1:301692/1‑151 (MQ=255)
                                                                                       cgcgcgGCGTGGAAGCCGGCCGCCTTCGGCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGTGCTCATCGt                                                            >  1:9128324/1‑151 (MQ=255)
                                                                                         cgcgGCGTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTcc                                                          <  2:11301006/151‑1 (MQ=255)
                                                                                              cgTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTcccgccc                                                     >  2:2218964/1‑151 (MQ=255)
                                                                                              cgTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTcccgccc                                                     <  1:6535552/151‑1 (MQ=255)
                                                                                              cgTGGAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTcccgccc                                                     <  2:4856177/151‑1 (MQ=255)
                                                                                                 ggAAGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCcgcg                                                  <  2:2299388/151‑1 (MQ=255)
                                                                                                   aaGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCa                                                >  1:4745266/1‑151 (MQ=255)
                                                                                                    aGCCGGCCGCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAg                                               >  2:7385915/1‑151 (MQ=255)
                                                                                                       cggccgCCTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGAGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCa                                            <  1:4868835/151‑1 (MQ=255)
                                                                                                          ccgccTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGtc                                         >  1:2866022/1‑151 (MQ=255)
                                                                                                          ccgccTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCGGAGCTCAGCGTCCCGCCCGCGCAGCCAGtc                                         >  1:2238068/1‑151 (MQ=255)
                                                                                                            gccTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGtcct                                       <  2:5256075/151‑1 (MQ=255)
                                                                                                             ccTTCGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGtcctc                                      >  2:589522/1‑151 (MQ=255)
                                                                                                                  gCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGTCCTCCAggg                                 <  1:1895900/151‑1 (MQ=255)
                                                                                                                  gCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGTCCTCCAggg                                 <  1:1189759/151‑1 (MQ=255)
                                                                                                                    cTGCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGTCCTCCAGGGcc                               >  2:7901013/1‑151 (MQ=255)
                                                                                                                      gCTCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGTCCTCCAGGGCCAg                             <  1:2000266/151‑1 (MQ=255)
                                                                                                                        tCCACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGTCCTCCAGGGCCAgcg                           <  1:8700465/151‑1 (MQ=255)
                                                                                                                         ccACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGTCCTCCAGGGCCAgcgg                          <  1:6826875/151‑1 (MQ=255)
                                                                                                                          cACCTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGTCCTCCAGGGCCAgcggc                         >  1:373177/1‑151 (MQ=255)
                                                                                                                            ccTCGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGTCCTCCAGGGCCAGcggcgg                       >  2:10172532/1‑151 (MQ=255)
                                                                                                                               cGGCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGTCCTCCAGGGCCAGCGGCggatg                    >  2:4727414/1‑151 (MQ=255)
                                                                                                                                ggCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGTCCTCCAGGGCCAGCGGCggatgg                   >  2:1659935/1‑151 (MQ=255)
                                                                                                                                 gCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGTCCTCCAGGGCCAGCGGCggatgga                  >  1:7728321/1‑151 (MQ=255)
                                                                                                                                 gCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGTCCTCCAGGGCCAGCGGCggatgga                  >  2:6783570/1‑151 (MQ=255)
                                                                                                                                 gCATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCAAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGTCCTCCAGGGCCAGCGGCggatgga                  >  2:9604861/1‑151 (MQ=255)
                                                                                                                                  cATCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGTCCTCCAGGGCCAGCGGCggatggat                 >  2:1968513/1‑151 (MQ=255)
                                                                                                                                    tCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGTCCTCCAGGGCCAGCGGCGGATGGATCa               >  2:1368578/1‑151 (MQ=255)
                                                                                                                                    tCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGTCCTCCAGGGCCAGCGGCGGATGGATCa               <  1:5189630/151‑1 (MQ=255)
                                                                                                                                    tCGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGTCCTCCAGGGCCAGCGGCGGATGGATCa               >  2:7523162/1‑151 (MQ=255)
                                                                                                                                     cGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGTCCTCCAGGGCCAGCGGCGGATGGATCAg              <  2:6911258/151‑1 (MQ=255)
                                                                                                                                     cGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGTCCTCCAGGGCCAGCGGCGGATGGATCAg              >  2:1301276/1‑151 (MQ=255)
                                                                                                                                     cGCTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGTCCTCCAGGGCCAGCGGCGGATGGATCAg              >  1:596297/1‑151 (MQ=255)
                                                                                                                                       cTCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGTCCTCCAGGGCCAGCGGCGGATGGATCAGCg            <  1:2655220/151‑1 (MQ=255)
                                                                                                                                        tCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGTCCTCCAGGGCCAGCGGCGGATGGATCAGCgg           >  2:10452187/1‑151 (MQ=255)
                                                                                                                                        tCAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGTCCTCCAGGGCCAGCGGCGGATGGATCAGCgg           >  2:599043/1‑151 (MQ=255)
                                                                                                                                         cAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGTCCTCCAGGGCCAGCGGCGGATGGATCAGCGGc          >  2:10539963/1‑151 (MQ=255)
                                                                                                                                         cAGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGTCCTCCAGGGCCAGCGGCGGATGGATCAGCGGc          <  1:1694506/151‑1 (MQ=255)
                                                                                                                                          aGGCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGTCCTCCAGGGCCAGCGGCGGATGGATCAGCGGCa         >  1:531645/1‑151 (MQ=255)
                                                                                                                                           ggCCGCCTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGTCCTCCAGGGCCAGCGGCGGATGGATCAGCGGCAg        <  1:5713065/151‑1 (MQ=255)
                                                                                                                                               gccTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGTGCTCATCGTCCCGCCCGCGCAGCCAGTCCTCCAGGGCCAGCGGCGGATGGATCAGCGGCTGGCTc    >  1:4585986/1‑151 (MQ=255)
                                                                                                                                                ccTTCGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGTCCTCCAGGGCCAGCGGCGGATGGATCAGCGGCAGGCTCg   >  2:7042679/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TATCAGGCGAAGTCGCCCCAGAGTTGCTGGGCCACCGCCAACGCTACCACCGGCGCGGTCTCGGTGCGCAGCACTCGCGGGCCGAGGCGCGCGGCGTGGAAGCCGGCCGCCTTTGCCTGCTCCACCTCGGCATCGCTCAGGCCGCCTTTGGGACCCACCAGGAAGGCCAGGCGCGACGGCGGCACATGGCTGGCCAGGGGGGCGGCCACAGGGTGCAGGACCAGCCTGAGCTCATCGTCCCGCCCGCGCAGCCAGTCCTCCAGGGCCAGCGGCGGATGGATCAGCGGCAGGCTCGA  >  NC_008463/480767‑481062

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑