Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 1,356,548 G→C R69G (CGT→GGT)  PA14_RS06375 ← MFS transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084631,356,5480GC99.8% 2182.5 / NA 561R69G (CGT→GGT) PA14_RS06375MFS transporter
Reads supporting (aligned to +/- strand):  ref base G (0/1);  major base C (263/296);  minor base A (0/1);  total (263/298)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 561 total aligned reads displayed.

ATGCTGTCGTAGCCGGGGAGGACGCCGATCAGGGTGGTGGATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACGGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCGATGGCGGTGCCGATGAAGCTGGCGACTGCGACCCG  >  NC_008463/1356400‑1356695
                                                                                                                                                    |                                                                                                                                                   
    tGTCGTAGCCGGGGAGGACGCCGATCAGGGTGGTGGATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGt                                                                                                                                               <  1:9101469/151‑1 (MQ=255)
     gTCGTAGCCGGGGAGGACGCCGATCAGGGTGGTGGATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTg                                                                                                                                              <  2:908107/151‑1 (MQ=255)
       cGTAGCCGGGGAGGACGCCGATCAGGGTGGTGGATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAg                                                                                                                                            <  2:9181744/151‑1 (MQ=255)
       cGTAGCCGGGGAGGACGCCGATCAGGGTGGTGGATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAg                                                                                                                                            <  2:10662046/151‑1 (MQ=255)
          aGCCGGGGAGGACGCCGATCAGGGTGGTGGATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGaa                                                                                                                                         <  2:2604590/151‑1 (MQ=255)
           gCCGGGGAGGACGCCGATCAGGGTGGTGGATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAg                                                                                                                                        >  2:6161047/1‑151 (MQ=255)
              ggggAGGACGCCGATCAGGGTGGTGGATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGgcg                                                                                                                                     <  2:8168340/151‑1 (MQ=255)
              ggggAGGACGCCGATCAGGGTGGTGGATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGgcg                                                                                                                                     <  1:3307367/151‑1 (MQ=255)
                    gaCGCCGATCAGGGTGGTGGATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGc                                                                                                                               <  1:10341180/151‑1 (MQ=255)
                     aCGCCGATCAGGGTGGTGGATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCa                                                                                                                              <  2:6901625/151‑1 (MQ=255)
                         cGATCAGGGTGGTGGATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTg                                                                                                                          >  1:10849310/1‑151 (MQ=255)
                          gATCAGGGTGGTGGATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTgc                                                                                                                         >  1:9012244/1‑151 (MQ=255)
                           aacAGGGTGGGGGATACCCCCATCCGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTgcg                                                                                                                        <  2:9715502/149‑1 (MQ=255)
                            tCAGGGTGGTGGATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTgcgc                                                                                                                       <  2:6313061/151‑1 (MQ=255)
                                 gtggtgGATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGc                                                                                                                  <  2:8589823/151‑1 (MQ=255)
                                      ggATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGAt                                                                                                             <  2:10002860/151‑1 (MQ=255)
                                       gATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATg                                                                                                            <  2:10127337/151‑1 (MQ=255)
                                       gATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTCGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATg                                                                                                            <  2:1612888/151‑1 (MQ=255)
                                        aTACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGt                                                                                                           <  1:2344523/151‑1 (MQ=255)
                                         tACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTc                                                                                                          <  2:11064963/151‑1 (MQ=255)
                                         tACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTc                                                                                                          >  1:678197/1‑151 (MQ=255)
                                             cccATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCg                                                                                                      >  2:7619447/1‑151 (MQ=255)
                                              ccATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCgg                                                                                                     >  2:7152063/1‑151 (MQ=255)
                                              ccATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCgg                                                                                                     >  2:3024796/1‑151 (MQ=255)
                                               cATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCggg                                                                                                    <  2:2328661/151‑1 (MQ=255)
                                                   agcagcagcGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGgaaga                                                                                                >  2:1046708/1‑151 (MQ=255)
                                                   agcagcagcGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGgaaga                                                                                                >  1:8761821/1‑151 (MQ=255)
                                                    gcagcagcGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGgaagaa                                                                                               <  1:7001752/151‑1 (MQ=255)
                                                     cagcagcGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAAt                                                                                              >  1:9737430/1‑151 (MQ=255)
                                                      agcagcGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGACGTGGCCGAACCGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATa                                                                                             <  2:5958433/151‑1 (MQ=255)
                                                      agcagcGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATa                                                                                             >  1:2918699/1‑151 (MQ=255)
                                                        cagcGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATAcc                                                                                           >  2:8863742/1‑151 (MQ=255)
                                                         agcGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCg                                                                                          <  1:8055181/151‑1 (MQ=255)
                                                           cGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGa                                                                                        >  2:8050469/1‑151 (MQ=255)
                                                              ggCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCg                                                                                     <  2:3256066/151‑1 (MQ=255)
                                                              ggCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCg                                                                                     <  1:7240227/151‑1 (MQ=255)
                                                               gCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGa                                                                                    >  1:7651810/1‑151 (MQ=255)
                                                                 gACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATc                                                                                  <  2:8405181/151‑1 (MQ=255)
                                                                 gACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATc                                                                                  >  1:5344355/1‑151 (MQ=255)
                                                                   ccAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCAc                                                                                >  1:8596425/1‑151 (MQ=255)
                                                                   ccAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCAc                                                                                >  1:1421955/1‑151 (MQ=255)
                                                                    cAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCAcc                                                                               >  1:3795073/1‑151 (MQ=255)
                                                                    cAGGGATGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATTCCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCAcc                                                                               <  2:10895053/151‑1 (MQ=255)
                                                                      gggTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAg                                                                             <  2:11428559/151‑1 (MQ=255)
                                                                       ggTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGATGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGGTAACCAgg                                                                            >  2:5468637/1‑151 (MQ=255)
                                                                        gTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAggg                                                                           >  1:7578994/1‑151 (MQ=255)
                                                                        gTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAggg                                                                           <  1:5728772/151‑1 (MQ=255)
                                                                             cTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGggcggcg                                                                      >  1:5543305/1‑151 (MQ=255)
                                                                             cTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGggcggcg                                                                      >  1:4690305/1‑151 (MQ=255)
                                                                             cTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGggcggcg                                                                      >  1:10602589/1‑151 (MQ=255)
                                                                               tGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGggcggcggc                                                                    <  1:7462228/151‑1 (MQ=255)
                                                                               tGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGggcggcggc                                                                    <  2:1803068/151‑1 (MQ=255)
                                                                                gcgcCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCa                                                                   <  2:3971184/151‑1 (MQ=255)
                                                                                 cgcCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAg                                                                  <  2:6604909/151‑1 (MQ=255)
                                                                                  gcCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGt                                                                 >  1:1590630/1‑151 (MQ=255)
                                                                                   cccGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTg                                                                <  2:7331256/151‑1 (MQ=255)
                                                                                   cccGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTg                                                                <  1:7335752/151‑1 (MQ=255)
                                                                                    ccGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTgg                                                               <  2:4946241/151‑1 (MQ=255)
                                                                                      gATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCa                                                             <  1:5071186/151‑1 (MQ=255)
                                                                                      gATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCa                                                             >  1:10709013/1‑151 (MQ=255)
                                                                                      gATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCa                                                             >  1:3484491/1‑151 (MQ=255)
                                                                                       aTGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCAt                                                            >  1:1013215/1‑151 (MQ=255)
                                                                                       aTGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCAt                                                            <  1:6057950/151‑1 (MQ=255)
                                                                                        tGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATa                                                           <  2:9413073/151‑1 (MQ=255)
                                                                                          cGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGTATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGa                                                         <  2:3661484/151‑1 (MQ=255)
                                                                                          cGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGa                                                         >  1:2233498/1‑151 (MQ=255)
                                                                                           ggTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGAc                                                        >  2:10241674/1‑151 (MQ=255)
                                                                                           ggTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGAc                                                        >  1:4610210/1‑151 (MQ=255)
                                                                                           ggTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGAc                                                        >  1:3642067/1‑151 (MQ=255)
                                                                                            gTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACg                                                       >  2:4537924/1‑151 (MQ=255)
                                                                                                 cGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGTCGGAGaa                                                  >  2:2151726/1‑151 (MQ=255)
                                                                                                       ggCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAACTCGTa                                            >  2:4655621/1‑151 (MQ=255)
                                                                                                         ccGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGa                                          <  1:791049/151‑1 (MQ=255)
                                                                                                            aaCAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAAtt                                       <  2:3112709/151‑1 (MQ=255)
                                                                                                            aaCAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAAtt                                       <  2:3496539/151‑1 (MQ=255)
                                                                                                             aCAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTc                                      <  1:980921/151‑1 (MQ=255)
                                                                                                              cAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCg                                     >  2:6770478/1‑151 (MQ=255)
                                                                                                               aGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCGa                                    >  2:10371046/1‑151 (MQ=255)
                                                                                                                 cgcCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCGATg                                  <  1:8264340/151‑1 (MQ=255)
                                                                                                                  gcCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCGATgg                                 >  1:179196/1‑151 (MQ=255)
                                                                                                                   ccGAGCCCAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCGATGGc                                <  1:2743398/151‑1 (MQ=255)
                                                                                                                        cccAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCGATGGCGGTGc                           >  1:4014824/1‑151 (MQ=255)
                                                                                                                        cccAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCGATGGCGGTGc                           >  1:4433405/1‑151 (MQ=255)
                                                                                                                        cccAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCGATGGCGGTGc                           >  1:5137989/1‑151 (MQ=255)
                                                                                                                          cAGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCGATGGCGGTGCCg                         <  1:2567114/151‑1 (MQ=255)
                                                                                                                           aGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCGATGGCGGTGCCGa                        >  1:3037686/1‑151 (MQ=255)
                                                                                                                           aGCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCGATGGCGGTGCCGa                        <  2:5695154/151‑1 (MQ=255)
                                                                                                                            gCGGGCGAGCGAGGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCGATGGCGGTGCCGAt                       <  1:9491839/151‑1 (MQ=255)
                                                                                                                               ggCGAGCGAGGAAGGCGATACAGACGGTGAGGAAGGCGCTGAGCTTCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGATATCGTAGAATTCGATGGCGGTGCCGTTGaa                    <  2:2935305/151‑1 (MQ=255)
                                                                                                                                 cgagcgagGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCGATGGCGGTGCCGATGAAGc                  >  1:11349508/1‑151 (MQ=255)
                                                                                                                                 cgagcgagGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCGATGGCGGTGCCGATGAAGc                  <  1:9224848/151‑1 (MQ=255)
                                                                                                                                   agcgagGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCGATGGCGGTGCCGATGAAGCTg                <  1:2120363/151‑1 (MQ=255)
                                                                                                                                   agcgagGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCGATGGCGGTGCCGATGAAGCTg                >  1:666946/1‑151 (MQ=255)
                                                                                                                                     cgagGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCGATGGCGGTGCCGATGAAGCTGGc              <  2:5129206/151‑1 (MQ=255)
                                                                                                                                     cgagGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACAAGGGCGGCGGACGTGGCATAGACGTAGAAATCGGAGAATGCGATGGCGGTGCCGATGAAGCTGGc              >  2:4587375/1‑151 (MQ=255)
                                                                                                                                      gagGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCGATGGCGGTGCCGATGAAGCTGGCg             >  1:8425368/1‑151 (MQ=255)
                                                                                                                                       agGAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGAGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAAGGCGGCGGCAGTGGCATAGACGTAGAAAACGTAGAAAACGATGGCGGTGCCGATGAACCTGCCGa            >  1:6454216/1‑151 (MQ=255)
                                                                                                                                         gAAGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCGATGGCGGTGCCGATGAAGCTGGCGACt          >  1:9357420/1‑151 (MQ=255)
                                                                                                                                          aaGGCGATACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCGATGGCGGTGCCGATGAAGCTGGCGACTg         >  2:4186091/1‑151 (MQ=255)
                                                                                                                                                 tACCGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCCGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCGATGGCGGAGCCGATGAAGCTGGCGACTGCGACCCg  >  1:11214344/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
ATGCTGTCGTAGCCGGGGAGGACGCCGATCAGGGTGGTGGATACCCCCATCAGCAGCAGCGAGGCGACCAGGGTTGACTTGCGCCCGATGCGGTCGCCGAAGTGGCCGAACAGCGCCGAGCCCAGCGGGCGAGCGAGGAAGGCGATACGGAAGGTGAGGAAGGCGCTGAGCATCTGCGCGGTGCCGGATGTCTGCGGGAAGAATACCGGACCGATCACCAGGGCGGCGGCAGTGGCATAGACGTAGAAATCGTAGAATTCGATGGCGGTGCCGATGAAGCTGGCGACTGCGACCCG  >  NC_008463/1356400‑1356695

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑