Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 1,558,205 A→G Q390Q (CAA→CAG)  PA14_RS07290 → propionyl‑CoA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084631,558,2050AG100.0% 2169.4 / NA 551Q390Q (CAA→CAG) PA14_RS07290propionyl‑CoA synthetase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (270/281);  total (270/281)

Only 100 of 552 total aligned reads displayed.

GCAAGGAAGACCCGCATGGCGAACGGGTGAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAAACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGGACGACGAAGGCCGGCCGCTGGGAGCGAACCGCCAGGGC  >  NC_008463/1558057‑1558352
                                                                                                                                                    |                                                                                                                                                   
 cAAGGAAGACCCGCATGGCGAACGGGTGAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGAcc                                                                                                                                                  <  2:2730376/151‑1 (MQ=255)
  aaGGAAGACCCGCATGGCGAACGGGTGAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCg                                                                                                                                                 <  2:2697495/151‑1 (MQ=255)
     gAAGACCCGCATGGCGAACGGGTGAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCgaga                                                                                                                                              <  2:3254230/151‑1 (MQ=255)
     gAAGACCCGCATGGCGAACGGGTGAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCgaga                                                                                                                                              <  2:4525598/151‑1 (MQ=255)
       aGACCCGCATGGCGAACGGGTGAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGAcc                                                                                                                                            <  1:6428214/151‑1 (MQ=255)
       aGACCCGCATGGCGAACGGGTGAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGAcc                                                                                                                                            <  2:1177301/151‑1 (MQ=255)
       aGACCCGCATGGCGAACGGGTGAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGAcc                                                                                                                                            >  2:8182846/1‑151 (MQ=255)
         aCCCGCATGGCGAACGGGTGAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCgg                                                                                                                                          <  2:9309953/151‑1 (MQ=255)
         aCCCGCATGGCGAACGGGTGAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCgg                                                                                                                                          >  1:11238601/1‑151 (MQ=255)
          cccGCATGGCGAACGGGTGAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGc                                                                                                                                         >  1:4218502/1‑151 (MQ=255)
              cATGGCGAACGGGTGAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGc                                                                                                                                     >  1:1906932/1‑151 (MQ=255)
              cATGGCGAACGGGTGAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGc                                                                                                                                     >  1:10133573/1‑151 (MQ=255)
                  gCGAACGGGTGAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGt                                                                                                                                 <  1:10770356/151‑1 (MQ=255)
                   cGAACGGGTGAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTg                                                                                                                                <  1:8788419/151‑1 (MQ=255)
                    gAACGGGTGAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGa                                                                                                                               >  2:66571/1‑151 (MQ=255)
                            gAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGccc                                                                                                                       >  2:1788912/1‑151 (MQ=255)
                            gAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGccc                                                                                                                       <  2:10622298/151‑1 (MQ=255)
                              aGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTg                                                                                                                     <  2:2962749/151‑1 (MQ=255)
                                 gcTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCAt                                                                                                                  >  1:3470221/1‑151 (MQ=255)
                                 gcTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCAt                                                                                                                  >  1:2502385/1‑151 (MQ=255)
                                 gcTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGCGCGGCAGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGctt                                                                                                                  >  1:9183736/1‑149 (MQ=255)
                                  cTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATc                                                                                                                 >  2:10101398/1‑151 (MQ=255)
                                   tACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCg                                                                                                                >  1:2717047/1‑151 (MQ=255)
                                     cGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCggg                                                                                                              >  1:8887315/1‑151 (MQ=255)
                                       aCCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGAt                                                                                                            <  1:3178832/151‑1 (MQ=255)
                                       aCCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGAt                                                                                                            <  1:4320485/151‑1 (MQ=255)
                                          tAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGc                                                                                                         >  1:7636664/1‑151 (MQ=255)
                                           aaGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGcc                                                                                                        <  1:4031270/151‑1 (MQ=255)
                                             gTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCgg                                                                                                      <  2:9930701/151‑1 (MQ=255)
                                              ttCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGc                                                                                                     >  2:1971855/1‑151 (MQ=255)
                                               tCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGcc                                                                                                    >  2:7160375/1‑151 (MQ=255)
                                               tCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGcc                                                                                                    <  2:6445425/151‑1 (MQ=255)
                                                cGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCa                                                                                                   <  2:6878480/151‑1 (MQ=255)
                                                 gCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCAc                                                                                                  <  1:11339268/151‑1 (MQ=255)
                                                     cgcCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGAcc                                                                                              >  2:7632677/1‑151 (MQ=255)
                                                     cgcCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGAcc                                                                                              <  2:8463995/151‑1 (MQ=255)
                                                       ccACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTg                                                                                            >  1:4706093/1‑151 (MQ=255)
                                                        cACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGc                                                                                           >  2:2206700/1‑151 (MQ=255)
                                                         aCCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCg                                                                                          <  1:3360413/151‑1 (MQ=255)
                                                          ccTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCgg                                                                                         >  1:7383160/1‑151 (MQ=255)
                                                            tGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGc                                                                                       >  1:6632751/1‑151 (MQ=255)
                                                             gTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGcc                                                                                      >  1:9642156/1‑151 (MQ=255)
                                                                ccTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGt                                                                                   >  2:43375/1‑151 (MQ=255)
                                                                  tGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTc                                                                                 >  1:7715155/1‑151 (MQ=255)
                                                                     ccGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGAc                                                                              >  2:740393/1‑151 (MQ=255)
                                                                      cGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGAcc                                                                             <  1:6096018/151‑1 (MQ=255)
                                                                       ggCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGTTGGCAGACCGAGACCGCATGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCa                                                                            >  1:5802888/1‑151 (MQ=255)
                                                                       ggCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCa                                                                            <  2:3938104/151‑1 (MQ=255)
                                                                       ggCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCa                                                                            >  1:5686136/1‑151 (MQ=255)
                                                                        gCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCaa                                                                           <  1:4113904/151‑1 (MQ=255)
                                                                         cGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAAc                                                                          >  1:5482422/1‑151 (MQ=255)
                                                                          gagaAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCTTCGGGATCGCCGGCCACGACCTGCGGGCCGGTGCGCCCAAcc                                                                         >  2:5976072/1‑151 (MQ=255)
                                                                           agaAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACcg                                                                        >  1:6842874/1‑151 (MQ=255)
                                                                                  cGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTg                                                                 <  2:3375857/151‑1 (MQ=255)
                                                                                   gACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGc                                                                >  1:8162387/1‑151 (MQ=255)
                                                                                   gACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGc                                                                >  2:3762465/1‑151 (MQ=255)
                                                                                     cagcagCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGccc                                                              <  1:5626919/151‑1 (MQ=255)
                                                                                      agcagcACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCg                                                             <  2:3020135/151‑1 (MQ=255)
                                                                                       gcagcaCCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCgg                                                            >  2:5274971/1‑151 (MQ=255)
                                                                                        cagcaCCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGc                                                           <  2:8702810/151‑1 (MQ=255)
                                                                                         agcaCCCAGCTCTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGAGCTTTGGTGGCTGTCCTAGTCGGGATGGCCGGTGTCATCGCCCTGCATCGGTATGGCCTGCCACGACCTGCGGGCCTGTTAGTCCTACCGCGCGTTGCCCTGCt                                                          <  2:11065800/151‑1 (MQ=255)
                                                                                          gcaCCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTa                                                         >  1:1357053/1‑151 (MQ=255)
                                                                                          gcaCCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTa                                                         <  1:5141165/151‑1 (MQ=255)
                                                                                            aCCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTAcc                                                       >  1:1754646/1‑151 (MQ=255)
                                                                                             cccAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCa                                                      <  2:1518201/151‑1 (MQ=255)
                                                                                               cAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACTACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCTCg                                                    <  1:8477372/151‑1 (MQ=255)
                                                                                                aGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCCCGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGt                                                   >  1:9351327/1‑151 (MQ=255)
                                                                                                aGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGt                                                   >  2:4912265/1‑151 (MQ=255)
                                                                                                  cACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGc                                                 >  2:466962/1‑151 (MQ=255)
                                                                                                   aCTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCa                                                >  1:2379569/1‑151 (MQ=255)
                                                                                                   aCTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCa                                                <  1:7906286/151‑1 (MQ=255)
                                                                                                    ctggctggAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAg                                               <  2:245183/151‑1 (MQ=255)
                                                                                                     tggctggAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAgg                                              >  2:10301696/1‑151 (MQ=255)
                                                                                                     tggctggAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGACGGTTACCGCGCCCTGCATCGGGATCGCCGGCAACGACCTGCGGGCCGGTTCGACCTACCGCGCGGTGCCAGTCTACCTCGTGCAgg                                              >  2:5155283/1‑151 (MQ=255)
                                                                                                       gctggAAGAGCACACCGGGCGGCCGTTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTg                                            <  2:9081069/151‑1 (MQ=255)
                                                                                                       gctggAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTg                                            <  1:3286806/151‑1 (MQ=255)
                                                                                                       gctggAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTg                                            <  1:8295128/151‑1 (MQ=255)
                                                                                                         tggAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCt                                          <  1:382359/151‑1 (MQ=255)
                                                                                                             agagCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGgac                                      <  2:561110/151‑1 (MQ=255)
                                                                                                               agCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGGTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGgacga                                    >  2:9510428/1‑151 (MQ=255)
                                                                                                                gCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGgacgac                                   <  2:7946521/151‑1 (MQ=255)
                                                                                                                   cacCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGGACGACGaa                                >  2:3564590/1‑151 (MQ=255)
                                                                                                                    acCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGGACGACGAAg                               >  1:4975787/1‑151 (MQ=255)
                                                                                                                    acCGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGGACGACGAAg                               >  1:7238899/1‑151 (MQ=255)
                                                                                                                      cGGGCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGGACGACGAAggc                             >  2:6362417/1‑151 (MQ=255)
                                                                                                                       gggCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGGACGACGAAggcc                            <  1:10402704/151‑1 (MQ=255)
                                                                                                                       gggCGGCCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGGACGACGAAggcc                            >  1:2123904/1‑151 (MQ=255)
                                                                                                                        ggcggcCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGGACGACGAAggccg                           >  1:3027598/1‑151 (MQ=255)
                                                                                                                        ggcggcCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGGACGACGAAggccg                           <  2:1135956/151‑1 (MQ=255)
                                                                                                                        ggcggcCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGGACGACGAAggccg                           <  2:9767788/151‑1 (MQ=255)
                                                                                                                           ggcCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGGACGACGAAggccggcc                        <  2:7816525/151‑1 (MQ=255)
                                                                                                                           ggcCGGTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGGACGACGAAggccggcc                        <  2:10727391/151‑1 (MQ=255)
                                                                                                                           ggcCGGTCCACGACCATTGGTGGCAGACCGAGACCGGATGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGACACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCTGCTACCACGTGCAGGTGCTGGACGACGAAggccggcc                        <  1:4565592/151‑1 (MQ=255)
                                                                                                                               ggTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGGACGACGAAGGCCGGCCGCTg                    <  2:8788595/151‑1 (MQ=255)
                                                                                                                                gTCCACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGGACGACGAAGGCCGGCCGCTgg                   <  2:5691987/151‑1 (MQ=255)
                                                                                                                                  ccACGACCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGGACGACGAAGGCCGGCCGCTGGGa                 >  2:5803883/1‑151 (MQ=255)
                                                                                                                                       aCCATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGGACGACGAAGGCCGGCCGCTGGGAGCGaa            >  2:6113439/1‑151 (MQ=255)
                                                                                                                                         cATTGGTGGCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGGACGACGAAGGCCGGCCGCTGGGAGCGAAcc          <  1:5880425/151‑1 (MQ=255)
                                                                                                                                            tggtggCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGGACGACGAAGGCCGGCCGCTGGGAGCGAACCGcc       >  2:72592/1‑151 (MQ=255)
                                                                                                                                            tggtggCAGACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGGACGACGAAGGCCGGCCGCTGGGAGCGAACCGcc       <  2:5906284/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GCAAGGAAGACCCGCATGGCGAACGGGTGAAGCGCTACGACCTAAGTTCGCTGCGCCACCTGTTCCTGGCCGGCGAGAAGCTCGACAGCAGCACCCAGCACTGGCTGGAAGAGCACACCGGGCGGCCGGTCCACGACCATTGGTGGCAAACCGAGACCGGCTGGCCGGTGACCGCGCCCTGCATCGGGATCGCCGGCCACGACCTGCGGGCCGGTTCGACCAACCGCGCGGTGCCCGGCTACCACGTGCAGGTGCTGGACGACGAAGGCCGGCCGCTGGGAGCGAACCGCCAGGGC  >  NC_008463/1558057‑1558352

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑