Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 2,149,425 T→C Q549Q (CAA→CAG)  PA14_RS09965 ← M14 family metallopeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084632,149,4250TC100.0% 1687.7 / NA 428Q549Q (CAA→CAG) PA14_RS09965M14 family metallopeptidase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (222/206);  total (222/206)

Only 100 of 428 total aligned reads displayed.

CGCGCAGGCGTTTGTCGAAGGCGGACTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTTTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGACCAGCAGGCGACCTTCGTAGCCCGGAATCCGGTTG  >  NC_008463/2149277‑2149572
                                                                                                                                                    |                                                                                                                                                   
 gcgcAGGCGTTTGTCGAAGGCGGACTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGc                                                                                                                                                  >  2:8717607/1‑151 (MQ=255)
 gcgcAGGCGTTTGTCGAAGGCGGACTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGc                                                                                                                                                  <  2:2294648/151‑1 (MQ=255)
 gcgcAGGCGTTTGTCGAAGGCGGACTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGc                                                                                                                                                  <  1:1976011/151‑1 (MQ=255)
 gcgcAGGCGTTTGTCGAAGGCGGACTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGc                                                                                                                                                  <  2:3737441/151‑1 (MQ=255)
  cgcAGGCGTTTGTCGAAGGCGGACTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCg                                                                                                                                                 <  1:6924933/151‑1 (MQ=255)
      ggCGTTTGTCGAAGGCGGACTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTg                                                                                                                                             >  2:823717/1‑151 (MQ=255)
      ggCGTTTGTCGAAGGCGGACTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTg                                                                                                                                             <  1:8732991/151‑1 (MQ=255)
      ggCGTTTGTCGAAGGCGGACTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTg                                                                                                                                             <  1:9425962/151‑1 (MQ=255)
        cGTTTGTCGAAGGCGGACTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTgca                                                                                                                                           <  1:4882951/151‑1 (MQ=255)
         gTTTGTCGAAGGCGGACTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTgcag                                                                                                                                          <  1:2669856/151‑1 (MQ=255)
          tttGTCGAAGGCGGACTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTgcagc                                                                                                                                         >  1:3484165/1‑151 (MQ=255)
           ttGTCGAAGGCGGACTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTgcagca                                                                                                                                        <  2:6990544/151‑1 (MQ=255)
           ttGTCGAAGGCGGACTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTgcagca                                                                                                                                        >  1:4942320/1‑151 (MQ=255)
            tGTCGAAGGCGGACTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGcagcag                                                                                                                                       <  1:6039067/151‑1 (MQ=255)
            tGTCGAAGGCGGACTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGcagcag                                                                                                                                       >  2:7136636/1‑151 (MQ=255)
            tGTCGAAGGCGGACTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGcagcag                                                                                                                                       >  1:11211680/1‑151 (MQ=255)
             gTCGAAGGCGGACTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAgg                                                                                                                                      <  1:9918141/151‑1 (MQ=255)
              tCGAAGGCGGACTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGt                                                                                                                                     <  1:2766112/151‑1 (MQ=255)
                  aGGCGGACTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCTGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGAt                                                                                                                                 <  2:4276409/151‑1 (MQ=255)
                      ggACTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGcc                                                                                                                             <  1:5158485/151‑1 (MQ=255)
                       gACTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCa                                                                                                                            <  2:4585081/151‑1 (MQ=255)
                        ccTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTCTTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAg                                                                                                                           <  2:6416243/150‑1 (MQ=255)
                        aCTTCAGCGCCGCGTCCTCCGCCAGCCTCTGGCGCGCCATCGGCTCCATCACGTCTCCCTCCGGCACCTCCGCGGCCACCCGCGAGGCATTGAAGAAGCCCCCGGACCAGACCGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAg                                                                                                                           <  2:302332/151‑1 (MQ=255)
                            cAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACg                                                                                                                       >  2:1834940/1‑151 (MQ=255)
                               cgcCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGccc                                                                                                                    >  1:4403623/1‑151 (MQ=255)
                               cgcCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGccc                                                                                                                    >  1:1641922/1‑151 (MQ=255)
                                gcCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCa                                                                                                                   <  2:7475545/151‑1 (MQ=255)
                                 ccGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAg                                                                                                                  <  2:10976893/151‑1 (MQ=255)
                                  cGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGc                                                                                                                 >  1:1242151/1‑151 (MQ=255)
                                  cGGGTCGTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTAGTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGc                                                                                                                 <  2:10837705/151‑1 (MQ=255)
                                   gggTCTTCCGCCCGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCg                                                                                                                >  2:5714457/1‑151 (MQ=255)
                                     gTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGc                                                                                                              >  1:3374026/1‑151 (MQ=255)
                                          ccGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGACCGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGc                                                                                                         <  2:9118337/151‑1 (MQ=255)
                                                gCATCTTGCGCGCCATCGGCTCCATCACGTAGTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGtc                                                                                                   <  1:2121926/151‑1 (MQ=255)
                                                 cATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGtcg                                                                                                  <  1:6567261/151‑1 (MQ=255)
                                                    cTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGtcgatc                                                                                               <  1:7384546/151‑1 (MQ=255)
                                                    cTTGCGCGCCATCGGCTCCATCACGGATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGtcgatc                                                                                               <  1:7620977/151‑1 (MQ=255)
                                                     ttGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGGGGACTTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGAGGGCCAGGACGCCCAGCGGCTGGGCGGTGtcgatcg                                                                                              >  2:4840408/1‑151 (MQ=255)
                                                         gcgcCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAg                                                                                          >  1:4075445/1‑151 (MQ=255)
                                                          cgcCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGc                                                                                         >  2:10585171/1‑151 (MQ=255)
                                                           gcCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCg                                                                                        <  2:9670072/151‑1 (MQ=255)
                                                             cATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAg                                                                                      >  1:2321958/1‑151 (MQ=255)
                                                              aTCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAgc                                                                                     <  2:4704128/151‑1 (MQ=255)
                                                              aTCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAgc                                                                                     >  1:2974777/1‑151 (MQ=255)
                                                                cGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAgccg                                                                                   <  2:8846831/151‑1 (MQ=255)
                                                                cGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAgccg                                                                                   >  2:9423954/1‑151 (MQ=255)
                                                                cGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAgccg                                                                                   >  2:9588311/1‑151 (MQ=255)
                                                                cGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAgccg                                                                                   <  2:4398851/151‑1 (MQ=255)
                                                                 ggCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGACTTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAgccgg                                                                                  >  1:968802/1‑151 (MQ=255)
                                                                 ggCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAgccgg                                                                                  >  1:6374513/1‑151 (MQ=255)
                                                                  gCTCCATCACGTAGTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAgccggc                                                                                 <  2:5836502/151‑1 (MQ=255)
                                                                   cTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAgccggcc                                                                                <  2:1543570/151‑1 (MQ=255)
                                                                    tCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAgccggccg                                                                               <  1:9126046/151‑1 (MQ=255)
                                                                          aCGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAgg                                                                         >  1:7297468/1‑151 (MQ=255)
                                                                            gTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTc                                                                       <  2:532475/151‑1 (MQ=255)
                                                                            gTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTc                                                                       <  2:519742/151‑1 (MQ=255)
                                                                             tATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGACGGTCt                                                                      >  2:1179603/1‑151 (MQ=255)
                                                                             tATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCt                                                                      >  1:9037713/1‑151 (MQ=255)
                                                                              aTTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTg                                                                     >  2:8425449/1‑151 (MQ=255)
                                                                                tcctccGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCa                                                                   >  1:8042461/1‑151 (MQ=255)
                                                                                tcctccGGCTCCTCCGCGGCCACCAGGGAGGAATTGAAGTAGCCCCAGGCCCAGAACGCAGCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCa                                                                   <  1:2226988/151‑1 (MQ=255)
                                                                                  ctccGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTAGCAGCAGGTAGATGTCACGGACGCACAGAGGCTGGGCTGTGACGATCGACAGCGAGCCGGCCGGGAAGGTCTGCacg                                                                 >  1:11132214/1‑149 (MQ=255)
                                                                                      ggCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCt                                                             >  1:8365998/1‑151 (MQ=255)
                                                                                       gCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCCGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGACGGGCTGCAAgcgcgg                                                            >  2:6718261/1‑149 (MQ=255)
                                                                                          cctcGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGaa                                                         >  2:10398401/1‑151 (MQ=255)
                                                                                              ggcggcCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGc                                                     >  1:3876217/1‑151 (MQ=255)
                                                                                              ggcggcCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGc                                                     >  2:8613548/1‑151 (MQ=255)
                                                                                                cggcCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCtt                                                   >  1:1799761/1‑151 (MQ=255)
                                                                                                cggcCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCtt                                                   <  2:3376688/151‑1 (MQ=255)
                                                                                                 ggcCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTc                                                  <  2:10126372/151‑1 (MQ=255)
                                                                                                 ggcCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTc                                                  >  1:7560445/1‑151 (MQ=255)
                                                                                                  gcCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCg                                                 <  2:2501092/151‑1 (MQ=255)
                                                                                                  gcCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCg                                                 >  2:11102952/1‑151 (MQ=255)
                                                                                                   ccaccaGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCgg                                                >  1:11264482/1‑151 (MQ=255)
                                                                                                    caccaGGGTTGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGc                                               <  2:6181040/151‑1 (MQ=255)
                                                                                                      ccaGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGt                                             <  2:9727525/151‑1 (MQ=255)
                                                                                                       caGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTg                                            >  1:6144122/1‑151 (MQ=255)
                                                                                                        aGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGc                                           >  1:10743593/1‑151 (MQ=255)
                                                                                                         gggTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGcc                                          <  1:10930649/151‑1 (MQ=255)
                                                                                                             ggAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTg                                      <  1:6993312/151‑1 (MQ=255)
                                                                                                              gAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACACTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGa                                     <  2:5537724/151‑1 (MQ=255)
                                                                                                               aaTTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGAc                                    >  1:10171531/1‑151 (MQ=255)
                                                                                                                  tgtagaagCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGACcag                                 >  1:7856327/4‑151 (MQ=255)
                                                                                                                      gaagCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGACCAGCAgg                             >  1:8143151/1‑151 (MQ=255)
                                                                                                                      gaagCCCCAGGACCAGAACGAATCCGGACTCTGCGGGTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGACCAGCAgg                             >  2:2752213/1‑151 (MQ=255)
                                                                                                                         gCCCCAGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGACCAGCAGGCGa                          >  1:100118/1‑151 (MQ=255)
                                                                                                                              aGGACCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGACCAGCAGGCGACCTTc                     >  1:9704643/1‑151 (MQ=255)
                                                                                                                                 aCCAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGACCAGCAGGCGACCTTCGTa                  >  1:4577254/1‑151 (MQ=255)
                                                                                                                                  ccAGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGACCAGCAGGCGACCTTCGTAg                 <  1:10397017/151‑1 (MQ=255)
                                                                                                                                    aGAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGACCAGCAGGCGACCTTCGTAGcc               <  1:6765260/151‑1 (MQ=255)
                                                                                                                                     gAACGAATCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGACCAGCAGGCGACCTTCGTAGccc              >  2:7952265/1‑151 (MQ=255)
                                                                                                                                           aTCCGGTCTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGTCAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGACCAGCAGGCGACCTTCGTTGCCCGGAATc        <  2:3200687/151‑1 (MQ=255)
                                                                                                                                            tCCGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGACCAGCAGGCGACCTTCGTAGCCCGGAATcc       >  2:9851930/1‑151 (MQ=255)
                                                                                                                                              cGGACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGACCAGCAGGCGACCTTCGTAGCCCGGAATCCgg     <  2:5036646/151‑1 (MQ=255)
                                                                                                                                               ggACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGACCAGCAGGCGACCTTCGTAGCCCGGAATCCGGt    >  2:11210990/1‑151 (MQ=255)
                                                                                                                                               ggACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGACCAGCAGGCGACCTTCGTAGCCCGGAATCCGGt    >  2:4039139/1‑151 (MQ=255)
                                                                                                                                               ggACTCGGCGGTCGCAGCAGGTCGAGGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGCCAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGACCAGCAGGCGACCTTCGTAGCCCGGAATCCGGt    <  1:5428919/151‑1 (MQ=255)
                                                                                                                                                gACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGACCAGCAGGCGACCTTCGTAGCCCGGAATCCGGtt   <  2:7754042/151‑1 (MQ=255)
                                                                                                                                                gACTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGACCAGCAGGCGACCTTCGTAGCCCGGAATCCGGtt   <  2:6028739/151‑1 (MQ=255)
                                                                                                                                                 aCTCTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGACCAGCAGGCGACCTTCGTAGCCCGGAATCCGGTTg  >  1:2607314/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CGCGCAGGCGTTTGTCGAAGGCGGACTTCAGCGCCGGGTCTTCCGCCAGCATCTTGCGCGCCATCGGCTCCATCACGTATTCCTCCGGCTCCTCGGCGGCCACCAGGGTGGAATTGAAGAAGCCCCAGGACCAGAACGAATCCGGACTTTGCGGTTGCAGCAGGTCGATGGCCAGGACGCCCAGCGGCTGGGCGGTGTCGATCGACAGCGAGCCGGCCGGGAAGGTCTGCAAGCGCTGGAACGGCTTCGGCGTGCCGCTGACCAGCAGGCGACCTTCGTAGCCCGGAATCCGGTTG  >  NC_008463/2149277‑2149572

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑