Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 2,354,149 A→G intergenic (‑34/+17) PA14_RS10945 ← / ← lipA lipase secretion chaperone/triacylglycerol lipase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084632,354,1490AG100.0% 1585.6 / NA 407intergenic (‑34/+17)PA14_RS10945/lipAlipase secretion chaperone/triacylglycerol lipase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (217/190);  total (217/190)

Only 100 of 407 total aligned reads displayed.

CCGCCTGCGCGCTGGCCGGGGGCGCCGTCTCGGGTGCGGGGGAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAAGCCCCGGCCGGGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAGGTGGTTCATCCGGTAGTTGTCGCGGAT  >  NC_008463/2354001‑2354297
                                                                                                                                                    |                                                                                                                                                    
   ccTGCGCGCTGGCCGGGGGCGCCGTCTCGGGTGCGGGGGAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGcccc                                                                                                                                                 >  1:237927/1‑151 (MQ=255)
   ccTGCGCGCTGGCCGGGGGCGCCGTCTCGGGTGCGGGGGAAGGGTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGTGGCCGAGGcccc                                                                                                                                                 <  1:9110695/151‑1 (MQ=255)
    cTGCGCGCTGGCCGGGGGCGCCGTCTCGGGTGCGGGGGAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCccg                                                                                                                                                <  1:3401794/151‑1 (MQ=255)
            tGGCCGGGGGCGCCGTCTCGGGTGCGGGGGAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCCggggt                                                                                                                                        >  1:1877669/1‑150 (MQ=255)
            tGGCCGGGGGCGCCGTCTCGGGTGCGGGGGAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCGTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCCggggt                                                                                                                                        >  2:10082529/1‑150 (MQ=255)
                cGGGGGCGCCGTCTCGGGTGCGGGGGAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTa                                                                                                                                   >  1:1664384/1‑151 (MQ=255)
                      cgcCGTCTCGGGTGCGGGGGAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGTATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCt                                                                                                                             >  1:6409064/1‑151 (MQ=255)
                       gcCGTCTCGGGTGCGGGGGAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTg                                                                                                                            >  2:9241903/1‑151 (MQ=255)
                        ccGTCTCGGGTGCGGGGGAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTgg                                                                                                                           >  2:1035055/1‑151 (MQ=255)
                         cGTCTCGGGTGCGGGGGAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGc                                                                                                                          >  2:7449686/1‑151 (MQ=255)
                           tctcGGGTGCGGGGGAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGt                                                                                                                        <  1:10355395/151‑1 (MQ=255)
                            ctcGGGTGCGGGGGAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGtt                                                                                                                       >  1:1127640/1‑151 (MQ=255)
                            ctcGGGTGCGGGGGAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGtt                                                                                                                       >  2:1555454/1‑151 (MQ=255)
                             tcGGGTGCGGGGGAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGttc                                                                                                                      <  2:1098487/151‑1 (MQ=255)
                              cGGGTGCGGGGGAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGttct                                                                                                                     >  2:8412852/1‑151 (MQ=255)
                               gggTGCGGGGGAAGGTTCCCGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGttctt                                                                                                                    <  1:9776296/151‑1 (MQ=255)
                                 gTGCGGGGGAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCa                                                                                                                  >  1:5472185/1‑151 (MQ=255)
                                  tGCGGGGGAAGGTTCCAGCCAGACGACCCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAg                                                                                                                 <  2:8648796/151‑1 (MQ=255)
                                   gCGGGGGAAGGTTCCAGCCCGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCGTCCCGCGCGGGGGCTGCCGGGACAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAgg                                                                                                                <  2:10532423/151‑1 (MQ=255)
                                      ggggAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCgg                                                                                                             >  1:6545960/1‑151 (MQ=255)
                                          aaGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTgg                                                                                                         >  1:6452881/1‑151 (MQ=255)
                                           aGGTTCCAGCCCGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGc                                                                                                        <  2:8755097/151‑1 (MQ=255)
                                               tCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGTATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGc                                                                                                    <  1:6257967/151‑1 (MQ=255)
                                                 cagccagACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGCGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTg                                                                                                  >  2:6634196/1‑151 (MQ=255)
                                                  agccagACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTgg                                                                                                 >  1:8041655/1‑151 (MQ=255)
                                                    ccagACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCg                                                                                               >  2:1697418/1‑151 (MQ=255)
                                                     cagACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCgg                                                                                              <  1:849836/151‑1 (MQ=255)
                                                      agACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGt                                                                                             >  2:8113647/1‑151 (MQ=255)
                                                       gACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTa                                                                                            >  2:2038589/1‑151 (MQ=255)
                                                       gACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAAACAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTa                                                                                            <  1:4688972/151‑1 (MQ=255)
                                                          gAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGAc                                                                                         <  1:10602743/151‑1 (MQ=255)
                                                           aaCCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACg                                                                                        >  1:4518379/1‑151 (MQ=255)
                                                             cccggccagccTGGCGGCGACCGCCAGTGGCCCCAGCAGGAGGACGGTCTACACGCGCGGGGCCGTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCAACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCt                                                                                      <  1:6853789/148‑1 (MQ=255)
                                                                  ccaggcTGGCGGCGAACGCCTGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCg                                                                                 <  1:4199716/151‑1 (MQ=255)
                                                                  ccaggcTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCg                                                                                 >  2:69237/1‑151 (MQ=255)
                                                                   caggcTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCgg                                                                                <  2:5187876/151‑1 (MQ=255)
                                                                    aggcTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCggg                                                                               >  1:3522348/1‑151 (MQ=255)
                                                                      gcTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCt                                                                             <  2:4902579/151‑1 (MQ=255)
                                                                        tGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTgg                                                                           <  1:3070533/151‑1 (MQ=255)
                                                                        tGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTgg                                                                           >  1:1960519/1‑151 (MQ=255)
                                                                        tGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTgg                                                                           >  1:9937525/1‑151 (MQ=255)
                                                                        tGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTgg                                                                           >  2:3530587/1‑151 (MQ=255)
                                                                         ggcggcGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGt                                                                          <  1:2623696/151‑1 (MQ=255)
                                                                          gcggcgACCGCCAGTGGAATCAGCAGGAGGATTTACTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGtc                                                                         <  1:393636/151‑1 (MQ=255)
                                                                          gcggcgAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGtc                                                                         >  2:10674771/1‑151 (MQ=255)
                                                                             gcgAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCg                                                                      >  2:5862966/1‑151 (MQ=255)
                                                                               gAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGaa                                                                    >  1:8885426/1‑151 (MQ=255)
                                                                               gAACGCCAGTGGAATCACCAGGAGGATTTACGTCACGCGTGGGGGCTTCCGGGACAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGaa                                                                    <  1:368636/151‑1 (MQ=255)
                                                                               gAACGCCAGTGGAAACAGCAGGAGGATTTACTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGaa                                                                    <  1:381167/151‑1 (MQ=255)
                                                                                aaCGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAAc                                                                   <  2:7097671/151‑1 (MQ=255)
                                                                                  cGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGGGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAg                                                                 <  1:7322255/151‑1 (MQ=255)
                                                                                  cGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAg                                                                 >  1:11404977/1‑151 (MQ=255)
                                                                                  cGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAg                                                                 >  1:4410431/1‑151 (MQ=255)
                                                                                  cGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAg                                                                 >  1:5949895/1‑151 (MQ=255)
                                                                                   gCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAgg                                                                >  1:8213498/1‑151 (MQ=255)
                                                                                     cAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCt                                                              <  1:8421306/151‑1 (MQ=255)
                                                                                      aGTGGAATCAGCTGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTg                                                             <  1:157004/151‑1 (MQ=255)
                                                                                      aGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTg                                                             >  1:3208890/1‑151 (MQ=255)
                                                                                           aaTCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAg                                                        >  2:7979498/1‑151 (MQ=255)
                                                                                            aTCTGCAGGTGGTTTTTCTTCTCGCGAGGGGGCTTCCGGTAAAGGGCGGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAgg                                                       <  1:10013453/151‑1 (MQ=255)
                                                                                            aTCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTCGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAgg                                                       >  1:1305193/1‑151 (MQ=255)
                                                                                             tCAGCAGGAGGAGTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGCCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCGGGTCTCGAACAGGCTGGTGAGGc                                                      >  1:8970608/1‑151 (MQ=255)
                                                                                             acagcagGAGGAGTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGGAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGc                                                      <  2:8816105/150‑1 (MQ=255)
                                                                                              cagcagGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGcc                                                     >  2:5804235/1‑151 (MQ=255)
                                                                                               agcagGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCg                                                    <  2:3375349/151‑1 (MQ=255)
                                                                                               agcagGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCg                                                    >  1:9604483/1‑151 (MQ=255)
                                                                                               agcagGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCg                                                    >  2:76378/1‑151 (MQ=255)
                                                                                                 cagGCGGATTTACTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGaa                                                  <  1:4917991/151‑1 (MQ=255)
                                                                                                  aggaggATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAg                                                 >  1:10750068/1‑151 (MQ=255)
                                                                                                    gaggaTTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGAc                                               <  2:5646262/151‑1 (MQ=255)
                                                                                                     aggaTTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGAcc                                              >  2:2494383/1‑151 (MQ=255)
                                                                                                      ggaTTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGGTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGAccg                                             >  2:9536886/1‑150 (MQ=255)
                                                                                                         ttttCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGt                                          <  2:1396059/151‑1 (MQ=255)
                                                                                                              gtcACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCAcc                                     <  2:4317565/150‑1 (MQ=255)
                                                                                                                cACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTc                                   <  1:9289151/151‑1 (MQ=255)
                                                                                                                  cgcgAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGt                                 >  1:5667188/1‑151 (MQ=255)
                                                                                                                  cgcgAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCGGGTGAGGCCGAAGACCTGGTTCACCTCGt                                 >  1:7766515/1‑151 (MQ=255)
                                                                                                                  cgcgAGGGGGCTACCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGt                                 >  1:8058150/1‑151 (MQ=255)
                                                                                                                    cgAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTTCAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTcc                               <  1:9415647/151‑1 (MQ=255)
                                                                                                                    cgAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTcc                               >  2:5276674/1‑151 (MQ=255)
                                                                                                                     gAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCa                              >  2:11270154/1‑151 (MQ=255)
                                                                                                                      aGGGGGCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAg                             >  2:5757363/1‑151 (MQ=255)
                                                                                                                        ggggCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAggt                           >  2:6710668/1‑151 (MQ=255)
                                                                                                                         gggCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAggtg                          >  1:7560212/1‑151 (MQ=255)
                                                                                                                           gCTTCCGGGCAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAggtggt                        <  1:7200347/151‑1 (MQ=255)
                                                                                                                           gCTTCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAggtggt                        <  2:10853294/151‑1 (MQ=255)
                                                                                                                             ttCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAGGTGGTTc                      >  1:875321/1‑151 (MQ=255)
                                                                                                                              tCCGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAGGTGGTTCa                     <  2:2939557/151‑1 (MQ=255)
                                                                                                                               ccGGGAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAGGTGGTTCAt                    >  2:3955063/1‑151 (MQ=255)
                                                                                                                                cGGGAATGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTTGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAGGTGGTTCATc                   <  1:11042549/151‑1 (MQ=255)
                                                                                                                                 gggAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAGGTGGTTCATcc                  >  1:5173825/1‑151 (MQ=255)
                                                                                                                                 gggAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAGGTGGTTCATcc                  <  2:3157936/151‑1 (MQ=255)
                                                                                                                                 gggAAAGGGCCGGGGCCGAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCGGGTGAGGCCGAAGACCTGGGTCACCTCGTCCAGGGGGTTCATcc                  >  2:4128434/1‑151 (MQ=255)
                                                                                                                                       gggccggggccgCGGCCCCGGCC‑GGGGTCCGACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAGGTGGTTCATCCGGTAGt            <  2:11165306/151‑1 (MQ=255)
                                                                                                                                         gccggggccgAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAGGTGGTTCATCCGGTAGTTg          <  2:2296005/151‑1 (MQ=255)
                                                                                                                                          ccggggccgAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAGGTGGTTCATCCGGTAGTTGt         >  2:2741478/1‑151 (MQ=255)
                                                                                                                                            ggggccgAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAGGTGGTTCATCCGGTAGTTGTcg       <  2:6303423/151‑1 (MQ=255)
                                                                                                                                               gccgAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCTGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCGGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAGGTGGTTCATCCGGTAGTTGTCGCgg    >  1:144597/1‑151 (MQ=255)
                                                                                                                                                ccgAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAGGTGGTTCATCCGGTAGTTGTCGCGGa   >  2:3636507/1‑151 (MQ=255)
                                                                                                                                                 cgAGGCCCCGGCC‑GGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAGGTGGTTCATCCGGTAGTTGTCGCGGAt  >  2:11398389/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CCGCCTGCGCGCTGGCCGGGGGCGCCGTCTCGGGTGCGGGGGAAGGTTCCAGCCAGACGAACCAGGCCAGGCTGGCGGCGAACGCCAGTGGAATCAGCAGGAGGATTTTCTTCACGCGAGGGGGCTTCCGGGAAAGGGCCGGGGCCGAAGCCCCGGCCGGGGGTCCTACAGGCTGGCGTTCTTCAGGCGGTTGGCGTGCTGGCGGTAGACGCTGACCGGGCTGGTCTCGAACAGGCTGGTGAGGCCGAAGACCTGGTTCACCTCGTCCAGGTGGTTCATCCGGTAGTTGTCGCGGAT  >  NC_008463/2354001‑2354297

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑