Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 2,407,463 A→G K125E (AAG→GAG)  PA14_RS11255 → glutathione S‑transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084632,407,4630AG99.8% 1796.4 / NA 460K125E (AAG→GAG) PA14_RS11255glutathione S‑transferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (205/254);  minor base T (0/1);  total (205/255)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 462 total aligned reads displayed.

CCGCTATGCGCCGGGCGACCTCTATCCGCAGGACCCGGTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACCCTGCGTACGCCGCCGGAGCAACGCGACAAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTGACATTCCCCTGGGCTGTTTCATCTACGCCTGGTTCGAGA  >  NC_008463/2407315‑2407610
                                                                                                                                                    |                                                                                                                                                   
ccgcTATGCGCCGGGCGACCTCTATCCGCAGGACCCGGTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAg                                                                                                                                                   >  2:4239614/1‑151 (MQ=255)
 cgcTATGCGCCGGGCGACCTCTATCCGCAGGACCCGGTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAgg                                                                                                                                                  >  2:1123572/1‑151 (MQ=255)
 cgcTATGCGCCGGGCGACCTCTATCCGCAGGACCCGGTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAgg                                                                                                                                                  <  2:8999640/151‑1 (MQ=255)
  gcTATGCGCCGGGCGACCTCTATCCGCAGGACCCGGTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGc                                                                                                                                                 >  2:4716029/1‑151 (MQ=255)
    tATGCGCCGGGCGACCTCTATCCGCAGGACCCGGTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGccc                                                                                                                                               <  1:11414872/151‑1 (MQ=255)
        cgcCGGGCGACCTCTCTCCGCAGGACCCGGTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGAt                                                                                                                                           <  2:8151638/151‑1 (MQ=255)
        cgcCGGGCGACCTCTATCCGCAGGACCCGGTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGAt                                                                                                                                           >  1:7847755/1‑151 (MQ=255)
        cgcCGGGCGACCTCTATCCGCAGGACCCGGTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGAt                                                                                                                                           <  2:2424736/151‑1 (MQ=255)
        cgcCGGGCGACCTCTATCCGCAGGACCCGGTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGAt                                                                                                                                           <  1:4003019/151‑1 (MQ=255)
         gcCGGGCGACCTCTATCCGCAGGACCCGGTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATc                                                                                                                                          >  2:274650/1‑151 (MQ=255)
         gcCGGGCGACCTCTATCCGCAGGACCCGGTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATc                                                                                                                                          <  2:5571568/151‑1 (MQ=255)
         gcCGGGCGACCTCTATCCGCAGGACCCGGTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATc                                                                                                                                          <  2:7620824/151‑1 (MQ=255)
          ccGGGCGACCTCGATCCGCAGGACCCGGTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCg                                                                                                                                         <  2:5870073/151‑1 (MQ=255)
           cGGGCGACCTCTATCCGCAGGACCCGGTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGc                                                                                                                                        <  1:1657801/151‑1 (MQ=255)
           cGGGCGACCTCTATCCGCAGGACCCGGTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGc                                                                                                                                        <  1:672087/151‑1 (MQ=255)
            gggCGACCTCTATCCGCAGGACCCGGTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCa                                                                                                                                       >  1:645896/1‑151 (MQ=255)
            gggCGACCTCTATCCGCAGGACCCGGTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCa                                                                                                                                       >  1:9407278/1‑151 (MQ=255)
              gCGACCTCTATCCGCAGGACCCGGTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCaaa                                                                                                                                     <  2:3559827/151‑1 (MQ=255)
               cGACCTCTATCCGCAGGACCCGGTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAg                                                                                                                                    >  2:844572/1‑151 (MQ=255)
               cGACCTCTATCCGCAGGACCCGGTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAg                                                                                                                                    >  1:8069340/1‑151 (MQ=255)
                  ccTCTATCCGCAGGACCCGGTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGgcg                                                                                                                                 <  1:10945226/151‑1 (MQ=255)
                  ccTCTATCCGCAGGACCCGGTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGgcg                                                                                                                                 <  2:977540/151‑1 (MQ=255)
                  ccTCTATCCGCAGGACCCGGTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGgcg                                                                                                                                 >  1:8738258/1‑151 (MQ=255)
                  ccTCTATCCGCAGGACCCGGTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGgcg                                                                                                                                 <  1:6973496/151‑1 (MQ=255)
                  ccTCTATCCGCAGGACCCGGTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGgcg                                                                                                                                 <  2:884720/151‑1 (MQ=255)
                   ctctATCCGCAGGACCCGGTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGgcgc                                                                                                                                <  2:2076235/151‑1 (MQ=255)
                      tATCCGCAGGACCCGGTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGc                                                                                                                             >  1:5042793/1‑151 (MQ=255)
                         ccGCAGGACCCGGTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGa                                                                                                                          <  1:1450218/151‑1 (MQ=255)
                            cAGGACCCGGTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCt                                                                                                                       <  1:2308408/151‑1 (MQ=255)
                            cAGGACCCGGTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCt                                                                                                                       <  2:10556947/151‑1 (MQ=255)
                             aGGACCCGGTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTg                                                                                                                      >  1:3263112/1‑151 (MQ=255)
                             aGGACCCGGTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTg                                                                                                                      <  1:10595086/151‑1 (MQ=255)
                               gACCCGGTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTgcg                                                                                                                    <  1:10029059/151‑1 (MQ=255)
                                     gTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGt                                                                                                              <  2:9604432/151‑1 (MQ=255)
                                      tGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGtt                                                                                                             <  2:1597039/151‑1 (MQ=255)
                                      tGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGtt                                                                                                             <  1:11298152/151‑1 (MQ=255)
                                      tGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGtt                                                                                                             <  2:3260210/151‑1 (MQ=255)
                                      tGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGtt                                                                                                             <  1:7296987/151‑1 (MQ=255)
                                       gcgccgcgccGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTtg                                                                                                            <  2:5769680/151‑1 (MQ=255)
                                       gcgccgcgccGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTtg                                                                                                            <  1:7400261/151‑1 (MQ=255)
                                        cgccgcgccgACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTtgc                                                                                                           >  1:7726681/1‑151 (MQ=255)
                                         gccgcgccgACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTtgct                                                                                                          >  1:7765853/1‑151 (MQ=255)
                                            gcgccgACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCg                                                                                                       <  1:4597221/151‑1 (MQ=255)
                                             cgccgacgccgaCAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCgg                                                                                                      <  2:10811632/151‑1 (MQ=255)
                                               ccgacgccgacCAGTGGAGGGACTGGACCCCCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCAGCGGCGAGTTGCTGCGGGt                                                                                                    <  2:2382866/151‑1 (MQ=255)
                                                cgacgccgacAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTg                                                                                                   <  2:6254651/151‑1 (MQ=255)
                                                  acgccgacAAGTGGATGGACTGGACCACATCGACTCTCGCCGGGACGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGACTGAGCAACGCGACTAGGCCCTGATCGCAAAGGCGCTTCAGACCTGCGGCGAGTTGCTGCGGGTGcc                                                                                                 <  2:403997/151‑1 (MQ=255)
                                                      cgacAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGAc                                                                                             <  2:4161057/151‑1 (MQ=255)
                                                       gacAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGAcc                                                                                            >  2:4828824/1‑151 (MQ=255)
                                                       gacAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGAcc                                                                                            >  1:8450197/1‑151 (MQ=255)
                                                             tggatggaCTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGAccc                                                                                      >  2:1906918/1‑151 (MQ=255)
                                                              ggatggaCTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCt                                                                                     <  2:5033284/151‑1 (MQ=255)
                                                                atggaCTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTgg                                                                                   <  2:4414444/151‑1 (MQ=255)
                                                                  ggactggacCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTggcc                                                                                 <  1:1080518/151‑1 (MQ=255)
                                                                      tggacCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTggccggcc                                                                             >  1:10107258/1‑151 (MQ=255)
                                                                        gacCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCaa                                                                           <  2:9127291/151‑1 (MQ=255)
                                                                          ccaccTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAAcc                                                                         >  1:6331633/1‑151 (MQ=255)
                                                                           caccTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAAccc                                                                        <  2:3976444/151‑1 (MQ=255)
                                                                             ccTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTg                                                                      <  2:9274755/151‑1 (MQ=255)
                                                                              cTCGACTCTCGCCGGGCCGTTCCTCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATAGCAAATGCGCTGCAGACCTGCGGCGAGTTGCTGCGTGTGCCTGACCAGACCCTGCCCGGCCAACCCTgt                                                                     >  2:8380916/1‑150 (MQ=255)
                                                                              cTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTgg                                                                     >  1:2890564/1‑151 (MQ=255)
                                                                                cGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCt                                                                   <  1:9066913/151‑1 (MQ=255)
                                                                                 gACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTg                                                                  >  1:5137036/1‑151 (MQ=255)
                                                                                   ctctCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTc                                                                <  1:5703892/151‑1 (MQ=255)
                                                                                        gCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCg                                                           >  2:6169493/1‑151 (MQ=255)
                                                                                        gCCGGGCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCg                                                           >  1:3565139/1‑151 (MQ=255)
                                                                                           gggCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAAc                                                        <  1:68280/151‑1 (MQ=255)
                                                                                           gggCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAAc                                                        <  1:65639/151‑1 (MQ=255)
                                                                                           gggCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAAc                                                        <  2:10378227/151‑1 (MQ=255)
                                                                                             gCCGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGc                                                      >  1:6097926/1‑151 (MQ=255)
                                                                                              ccGTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCt                                                     <  2:5674556/151‑1 (MQ=255)
                                                                                                gTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTc                                                   >  2:1735081/1‑151 (MQ=255)
                                                                                                gTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTc                                                   >  1:3502121/1‑151 (MQ=255)
                                                                                                gTTCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTc                                                   >  2:10042465/1‑151 (MQ=255)
                                                                                                  tCCGCGACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCgg                                                 <  2:2893985/151‑1 (MQ=255)
                                                                                                      cgACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATg                                             <  2:10095874/151‑1 (MQ=255)
                                                                                                       gACCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATgg                                            >  2:6728023/1‑151 (MQ=255)
                                                                                                        cccTGTTCTGGGGACCGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATggg                                           <  2:7280167/150‑1 (MQ=255)
                                                                                                        aCCTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATggg                                           <  2:1167668/151‑1 (MQ=255)
                                                                                                          cTGTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTg                                         <  2:711407/151‑1 (MQ=255)
                                                                                                            gTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTGAc                                       >  1:4229603/1‑151 (MQ=255)
                                                                                                            gTTCTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTGAc                                       >  1:2651195/1‑151 (MQ=255)
                                                                                                               cTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTGACAtt                                    <  2:5187526/151‑1 (MQ=255)
                                                                                                               cTGGGGAACGCTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTGACAtt                                    >  1:9786903/1‑151 (MQ=255)
                                                                                                                        gcTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTGACATTCCCCTGGGc                           <  1:1512335/150‑1 (MQ=255)
                                                                                                                         cTGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTGACATTCCCCTGGGCt                          >  1:4305939/1‑151 (MQ=255)
                                                                                                                          tGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTGACATTCCCCTGGGCTg                         <  2:11082034/151‑1 (MQ=255)
                                                                                                                          tGCGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTGACATTCCCCTGGGCTg                         <  2:7686731/151‑1 (MQ=255)
                                                                                                                            cGTACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTGACATTCCCCTGGGCTGtt                       <  1:3133401/151‑1 (MQ=255)
                                                                                                                              tACGCCGCCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTGACATTCCCCTGGGCTGTTTc                     >  2:5384182/1‑151 (MQ=255)
                                                                                                                                cgccgcCGGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTGACATTCCCCTGGGCTGTTTCAt                   <  1:5624340/151‑1 (MQ=255)
                                                                                                                                  ccgccgGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTGACATTCCCCTGGGCTGTTTCATCt                 <  2:1485677/151‑1 (MQ=255)
                                                                                                                                    gccgGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTGACATTCCCCTGGGCTGTTTCATCTAc               >  1:2200292/1‑151 (MQ=255)
                                                                                                                                    gccgGAGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTGACATTCCCCTGGGCTGTTTCATCTAc               <  2:6428355/151‑1 (MQ=255)
                                                                                                                                         aGCAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTGACATTCCCCTGGGCTGTTTCATCTACGCCTg          <  1:1850190/151‑1 (MQ=255)
                                                                                                                                           cAACGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTGACATTCCCCTGGGCTGTTTCATCTACGCCTGGt        >  2:3824505/1‑151 (MQ=255)
                                                                                                                                            aaCGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTGACATTCCCCTGGGCTGTTTCATCTACGCCTGGtt       >  2:7932939/1‑151 (MQ=255)
                                                                                                                                            aaCGCGACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTGACATTCCCCTGGGCTGTTTCATCTACGCCTGGtt       <  1:1617931/151‑1 (MQ=255)
                                                                                                                                              cgcgACGAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTGACATTCCCCTGGGCTGTTTCATCTACGCCTGGTTCg     >  2:1095585/1‑151 (MQ=255)
                                                                                                                                                cgacgaGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCGGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTGCGGCTTGGGGGACCTTCCCCTGGGCTGTGTCATCTACGCCTGGTTCgag   >  2:2559633/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CCGCTATGCGCCGGGCGACCTCTATCCGCAGGACCCGGTGCGCCGCGCCGACGCCGACAAGTGGATGGACTGGACCACCTCGACTCTCGCCGGGCCGTTCCGCGACCTGTTCTGGGGAACCCTGCGTACGCCGCCGGAGCAACGCGACAAGGCCCTGATCGCAAAGGCGCTGCAGACCTGCGGCGAGTTGCTGCGGGTGCCGGACCAGACCCTGGCCGGCCAACCCTGGCTGTCCGGCGAACGCTTCGGCATGGGTGACATTCCCCTGGGCTGTTTCATCTACGCCTGGTTCGAGA  >  NC_008463/2407315‑2407610

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑