Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 791,862 3 bp→GCT coding (1842‑1844/5430 nt) pchF → pyochelin non‑ribosomal peptide synthetase PchF

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_008463791,8620CG99.8% 2098.8 / NA 541A614A (GCC→GCG) pchFpyochelin non‑ribosomal peptide synthetase PchF
Reads supporting (aligned to +/- strand):  ref base C (1/0);  major base G (325/214);  minor base T (0/1);  total (326/215)
Fisher's exact test for biased strand distribution p-value = 3.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*NC_008463791,8630TC99.8% 2103.7 / NA 541W615M (TGG→CTG) pchFpyochelin non‑ribosomal peptide synthetase PchF
Reads supporting (aligned to +/- strand):  ref base T (1/0);  major base C (324/215);  minor base A (1/0);  total (326/215)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*NC_008463791,8640GT99.6% 2250.6 / NA 540W615M (TGG→CTG) pchFpyochelin non‑ribosomal peptide synthetase PchF
Reads supporting (aligned to +/- strand):  ref base G (0/2);  major base T (324/212);  minor base A (1/1);  total (325/215)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 541 total aligned reads displayed.

TCGCGGTGATGCTGCCGAAAAGCGCCGCGCAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCCCCGAAGTGGTCGCCTGGGTCTGCCTGGAAAGCGATGTCCCGAACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGGACGACCTCGCCTACGTGATCTACACCTCCGG  >  NC_008463/791720‑792005
                                                                                                                                              |||                                                                                                                                             
tCGCGGTGATGCTGCCGAAAAGCGCCGCGCAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTCTGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTg                                                                                                                                         <  1:4110904/151‑1 (MQ=255)
  gcgGTGATGCTGCCGAAAAGCGCCGCGCAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGcc                                                                                                                                       >  1:3056608/1‑151 (MQ=255)
   cgGTGATGCTGCCGAAAAGCGCCGCGCAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCGATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGTTCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGcgg                                                                                                                                      >  2:9724528/1‑149 (MQ=255)
        aTGCTGCCGAAAAGCGCCGCGCAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGaaa                                                                                                                                 >  1:8012897/1‑151 (MQ=255)
        aTGCTGCCGAAAAGCGCCGCGCAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGaaa                                                                                                                                 >  1:5661899/1‑151 (MQ=255)
         tgctgcCGAAAAGCGCCGCGCAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAg                                                                                                                                >  1:2913482/1‑151 (MQ=255)
         tgctgcCGAAAAGCGCCGCGCAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAg                                                                                                                                >  1:3765973/1‑151 (MQ=255)
         tgctgcCGAAAAGCGCCGCGCAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAg                                                                                                                                >  1:8960993/1‑151 (MQ=255)
         agctgcCGAACAGCGCCGCGCACTTGGCCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTAAGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGCTCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAg                                                                                                                                <  2:11343875/150‑1 (MQ=255)
          gctgcCGAAAAGCGCCGCGCAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGc                                                                                                                               >  2:5255193/1‑151 (MQ=255)
             gcCGAAAAGCGCCGCGCAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGAt                                                                                                                            >  1:11110587/1‑151 (MQ=255)
             gcCGAAAAGCGCCGCGCAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGAt                                                                                                                            >  2:2125018/1‑151 (MQ=255)
               cGAAAAGCGCCGCGCAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGt                                                                                                                          <  1:2631640/151‑1 (MQ=255)
                  aaaGCGCCGCGCAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTccc                                                                                                                       >  2:9083045/1‑151 (MQ=255)
                  aaaGCGCCGCGCAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTccc                                                                                                                       >  2:5473347/1‑151 (MQ=255)
                   aaGCGCCGCGCAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCg                                                                                                                      >  1:10036668/1‑151 (MQ=255)
                    aGCGCCGCGCAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCAGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCgg                                                                                                                     >  1:6973856/1‑150 (MQ=255)
                     gcgcCGCGCAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCgga                                                                                                                    <  2:219694/151‑3 (MQ=255)
                      cgccgcGCAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGAc                                                                                                                   >  1:1949402/1‑151 (MQ=255)
                      cgccgcGCAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGAc                                                                                                                   >  1:10139575/1‑151 (MQ=255)
                       gccgcGCAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACg                                                                                                                  <  1:5370549/151‑1 (MQ=255)
                        ccgcGCAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGc                                                                                                                 >  1:4310001/1‑151 (MQ=255)
                         cgcgCAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGAGCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGCAAGCGATGTCCCGGACGcc                                                                                                                >  2:8302867/1‑151 (MQ=255)
                          gcgcAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGTCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCg                                                                                                               <  1:7869118/151‑1 (MQ=255)
                          gcgcAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCg                                                                                                               <  2:5663612/151‑1 (MQ=255)
                           cgcAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCgg                                                                                                              >  2:1438196/1‑151 (MQ=255)
                           cgcAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCgg                                                                                                              <  2:9286216/151‑1 (MQ=255)
                               aTTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTgc                                                                                                          >  2:1493557/1‑151 (MQ=255)
                                   gTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCt                                                                                                      <  2:3685936/151‑1 (MQ=255)
                                      gcgGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCg                                                                                                   >  1:3283088/1‑151 (MQ=255)
                                       cgGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAACGCGATGTCCCGGACGCCGGCTGCGCCTGCGt                                                                                                  >  1:1313843/1‑151 (MQ=255)
                                         gTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTgg                                                                                                >  1:9729698/1‑151 (MQ=255)
                                         gTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTgg                                                                                                <  1:5827492/151‑1 (MQ=255)
                                          tGATCGGCATCCTCCAGGCCGGCGCAGCCTCTGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGACAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGc                                                                                               >  2:2688785/1‑151 (MQ=255)
                                          tGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGc                                                                                               <  1:6123613/151‑1 (MQ=255)
                                          tGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGc                                                                                               >  1:75548/1‑151 (MQ=255)
                                          tGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGc                                                                                               >  1:2810225/1‑151 (MQ=255)
                                          tGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGc                                                                                               >  2:6283297/1‑151 (MQ=255)
                                           gATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGcc                                                                                              >  2:7905928/1‑151 (MQ=255)
                                            aTCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCa                                                                                             >  2:1949527/1‑151 (MQ=255)
                                               ggCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCg                                                                                          >  1:623674/1‑151 (MQ=255)
                                               ggCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCg                                                                                          >  1:10556477/1‑151 (MQ=255)
                                               ggCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCAGCg                                                                                          >  2:5432884/1‑151 (MQ=255)
                                                 cATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGAc                                                                                        <  2:8876969/151‑1 (MQ=255)
                                                  aTCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGAcc                                                                                       >  1:4681080/1‑151 (MQ=255)
                                                  aTCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGGGGTCGCGCTGGTCTGCCTGGAAAGCGCTGTCCCGGACGCCGGCTGCGCCTGCGTGGCCAGCGAcc                                                                                       >  2:10319690/1‑151 (MQ=255)
                                                   tcctccAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCg                                                                                      >  2:4382087/1‑151 (MQ=255)
                                                        cAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTgg                                                                                 <  1:6487295/151‑1 (MQ=255)
                                                        cAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCACGGAGGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTgg                                                                                 <  1:9788858/151‑1 (MQ=255)
                                                             cGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGACAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGgccgcc                                                                            >  2:6707253/1‑151 (MQ=255)
                                                             cGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGgccgcc                                                                            >  2:6097801/1‑151 (MQ=255)
                                                              ggCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGccgccg                                                                           >  2:1760186/1‑151 (MQ=255)
                                                               gcgcAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGa                                                                          >  2:7745270/1‑151 (MQ=255)
                                                                cgcAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGAc                                                                         >  1:1839014/1‑151 (MQ=255)
                                                                     ccTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGCAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAgcgc                                                                    >  1:8354800/1‑151 (MQ=255)
                                                                      cTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGcc                                                                   >  1:10252035/1‑151 (MQ=255)
                                                                       tATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCt                                                                  <  1:4429800/151‑1 (MQ=255)
                                                                       tATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCACGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCAGCGACCGGCTGGCCGCCGACAGCGCCt                                                                  <  2:10778820/151‑1 (MQ=255)
                                                                          gtgCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGgc                                                               >  1:6694533/1‑151 (MQ=255)
                                                                          gtgCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGgc                                                               >  1:9878170/1‑151 (MQ=255)
                                                                           tgCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGgcc                                                              <  2:3213481/151‑1 (MQ=255)
                                                                              cGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGgccgcc                                                           >  2:3399194/1‑151 (MQ=255)
                                                                               ggTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCa                                                          >  1:5052227/1‑151 (MQ=255)
                                                                                  ggACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCAccg                                                       >  1:6081533/1‑151 (MQ=255)
                                                                                    acctCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGACAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCAccgcc                                                     >  1:4924812/4‑151 (MQ=255)
                                                                                    aCATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCAccgcc                                                     >  1:1175136/1‑151 (MQ=255)
                                                                                     cATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCAccgccc                                                    <  2:2999964/151‑1 (MQ=255)
                                                                                     cATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCAccgccc                                                    >  2:6417288/1‑151 (MQ=255)
                                                                                      aTCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGACAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCAccgcccg                                                   >  2:5822968/1‑151 (MQ=255)
                                                                                      aTCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCAccgcccg                                                   >  1:9349228/1‑151 (MQ=255)
                                                                                       tCCGCCAGCCGCCGCTGCGGCGCCTGGCGATCCTCGCCTGCGCCGAAGGGGTCGCGCTGGTCGGCCTGGAAAGCGATGTCCGGGACGCCGGCTGCGCCTGCGTGGCCAGCGACGGGCTGGCCGCCGACTGCGCCTGGCCGCCAccgcccgc                                                  <  2:2442611/151‑1 (MQ=255)
                                                                                         cGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCgcgg                                                >  2:848246/1‑151 (MQ=255)
                                                                                          gCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCgcggc                                               <  2:4713014/151‑1 (MQ=255)
                                                                                          gCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCAGGTCTGCCTGGAAAGCGAGGGCCCGGACCCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCgcggc                                               <  1:7187785/151‑1 (MQ=255)
                                                                                           ccAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCgcggcg                                              >  2:7131654/1‑151 (MQ=255)
                                                                                             aGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGa                                            <  1:7819019/151‑1 (MQ=255)
                                                                                             aGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGa                                            <  2:4129104/151‑1 (MQ=255)
                                                                                             aGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGa                                            >  2:7620262/1‑151 (MQ=255)
                                                                                               ccgccgCTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAgg                                          <  2:3132952/151‑1 (MQ=255)
                                                                                                cgccgcTGCGGCGCCAGGCGATCCTCGCCAGCGCCGACGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGt                                         >  2:10184512/1‑151 (MQ=255)
                                                                                                cgccgcTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGt                                         >  2:6939157/1‑151 (MQ=255)
                                                                                                cgccgcTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGt                                         >  2:4538801/1‑151 (MQ=255)
                                                                                                cgccgcTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGt                                         >  2:1157802/1‑151 (MQ=255)
                                                                                                   cgcTGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTggc                                      <  1:3463147/151‑1 (MQ=255)
                                                                                                      tGCGGCGCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTggcggc                                   <  2:5002581/151‑1 (MQ=255)
                                                                                                         ggcgCCAGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGga                                <  2:10577137/151‑1 (MQ=255)
                                                                                                              cAGGCGATCCTCGCCAGCGCCGCAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGGACGAcc                           <  2:3881085/151‑1 (MQ=255)
                                                                                                               aGGCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGGACGACCt                          <  1:1390831/151‑1 (MQ=255)
                                                                                                                tgccaTCCTCGCCCGCGCCGATGTGGTCGCGCAGGTCTGCCTGGAAAGCGATCACCCAGACGCCGGCTGCGCCTGCGTGGCCATCGACAGGCTGGCAGCCCACAGCTACTGGCCGCCAACGCCCCCGCCGGAGGTCGCGGCGgacgacagc                         >  2:11156032/5‑148 (MQ=255)
                                                                                                                ggCGATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGGACGACCTc                         >  2:9888395/1‑151 (MQ=255)
                                                                                                                   gATCCTCGCCAGCGCTGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGGACGACCTCGcc                      >  1:1667949/1‑151 (MQ=255)
                                                                                                                   gATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGGACGACCTCGcc                      <  1:8726878/151‑1 (MQ=255)
                                                                                                                   gATCCTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGGACGACCTCGcc                      >  2:10500672/1‑151 (MQ=255)
                                                                                                                      ccTCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGGACGACCTCGCCGAc                   <  1:2207342/151‑1 (MQ=255)
                                                                                                                        tCGCCAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGGACGACCTCGCCTACGt                 <  1:10711785/151‑1 (MQ=255)
                                                                                                                            cAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGGACGACCTCGCCTACGTGATc             <  2:7360031/151‑1 (MQ=255)
                                                                                                                            cAGCGCCGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGGACGACCTCGCCTACGTGATc             >  2:4829127/1‑151 (MQ=255)
                                                                                                                               cgccGAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGGACGACCTCGCCTACGTGATCTac          >  1:9482064/3‑151 (MQ=255)
                                                                                                                                   gAAGTGGTCGCGCTGGTCTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGGACGACCTCGCCTACGTGATCTACACCt      >  2:8565860/1‑151 (MQ=255)
                                                                                                                                      gTGGTCGCGCTGGACTGCCTGGAAAGCGATGTCCCGGACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGGACGACCTCGCCTACGTGATCTACACCTCCg   <  1:10905309/151‑1 (MQ=255)
                                                                                                                                              |||                                                                                                                                             
TCGCGGTGATGCTGCCGAAAAGCGCCGCGCAATTGGTCGCGGTGATCGGCATCCTCCAGGCCGGCGCAGCCTATGTGCCGGTGGACATCCGCCAGCCGCCGCTGCGGCGCCAGGCGATCCTCGCCAGCCCCGAAGTGGTCGCCTGGGTCTGCCTGGAAAGCGATGTCCCGAACGCCGGCTGCGCCTGCGTGGCCATCGACCGGCTGGCCGCCGACAGCGCCTGGCCGCCACCGCCCGCGGCGGAGGTGGCGGCGGACGACCTCGCCTACGTGATCTACACCTCCGG  >  NC_008463/791720‑792005

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑